专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20040106145A1 Method and apparatus for the automated generation of nucleic acid ligands 有权
标题翻译：用于自动生成核酸配体的方法和装置
公开(公告)号：US20040106145A1
公开(公告)日：2004-06-03
申请号：US10717105
申请日：2003-11-18
申请人： SOMALOGIC, INC.
发明人： Larry Gold , Dominic A. Zichi , Robert D. Jenison , Daniel J. Schneider
IPC分类号： C12Q001/68 , G06F019/00 , G01N033/48 , G01N033/50
CPC分类号： C12N15/1048
摘要： The present invention includes a method and device for performing automated SELEX. The steps of the SELEX process are performed at one or more work stations on a work surface by a robotic manipulator controlled by a computer. The invention also includes methods and reagents to obviate the need for size-fractionation of amplified candidate nucleic acids before beginning the next round of the SELEX process.
摘要翻译：本发明包括用于执行自动化SELEX的方法和装置。 SELEX过程的步骤由工作表面上的一个或多个工作站由计算机控制的机器人操纵器执行。本发明还包括在开始下一轮SELEX过程之前消除对扩增候选核酸的大小分级的需要的方法和试剂。

2. 发明申请

US20030162216A1 Nucleic acid ligand diagnostic biochip 有权
标题翻译：核酸配体诊断生物芯片
公开(公告)号：US20030162216A1
公开(公告)日：2003-08-28
申请号：US10375487
申请日：2003-02-27
申请人： SomaLogic, Inc.
发明人： Larry Gold , Dominic A. Zichi
IPC分类号： C12Q001/68 , G01N033/53 , G01N033/542 , C12M001/34
CPC分类号： C12Q1/6839 , C07B2200/11 , C12Q1/6834 , C12Q1/6837 , C40B40/00 , Y10S977/924 , C12Q2565/514 , C12Q2565/101 , C12Q2541/101 , C12Q2525/205
摘要： A nucleic acid ligand nullbiochipnull is disclosed, consisting of a solid support to which one or more specific nucleic acid ligands is attached in a spatially defined manner. Each nucleic acid ligand binds specifically and avidly to a particular target molecule contained within a test mixture, such as a bodily fluid. The target molecules include, but are not limited to, proteins (cellular, viral, bacterial, etc.) hormones, sugars, metabolic byproducts, cofactor, and intermediates, drugs, and toxins. Contacting the test mixture with the biochip leads to the binding of a target molecule to its cognate nucleic acid ligand. The biochip may then be contacted with a reagent(s) that reacts covalently with proteins and not with nucleic acids. Each protein target in the test mixture may then detected by detecting the presence of the reagent at the appropriate address on the biochip.
摘要翻译：公开了一种核酸配体“生物芯片”，其由以空间限定的方式连接一个或多个特定核酸配体的固体支持物组成。每个核酸配体特异性地并且强烈地结合到测试混合物（例如体液）中所含的特定靶分子。靶分子包括但不限于蛋白质（细胞，病毒，细菌等）激素，糖，代谢副产物，辅因子和中间体，药物和毒素。将测试混合物与生物芯片接触导致靶分子与其同源核酸配体的结合。然后生物芯片可以与与蛋白质共价反应的试剂与不与核酸反应的试剂接触。然后可以通过检测生物芯片上适当地址处的试剂的存在来检测测试混合物中的每个蛋白质靶标。

3. 发明申请

US20040132067A1 Systematic evolution of ligands by exponential enrichment: photoselection of nucleic acid ligands and solution selex 审中-公开
标题翻译：配体通过指数富集的系统演化：核酸配体的照片选择和溶液溶解
公开(公告)号：US20040132067A1
公开(公告)日：2004-07-08
申请号：US10681822
申请日：2003-10-08
申请人： SOMALOGIC, INC.
发明人： Larry Gold , Michael Willis , Tad Koch , Steven Ringquist , Kirk Jensen , Brent Atkinson
IPC分类号： C12Q001/68 , C07H021/04 , C12P019/34
CPC分类号： G01N33/76 , B82Y5/00 , C07H19/06 , C07H19/10 , C07H21/00 , C07K14/001 , C12N9/1276 , C12N15/1048 , C12N2310/13 , C12N2310/322 , C12N2310/53 , C12Q1/37 , C12Q1/6804 , C12Q1/6811 , C12Q1/70 , C12Q1/703 , C40B40/00 , F02B2075/027 , G01N33/531 , G01N33/532 , G01N33/535 , G01N33/56988 , G01N33/68 , G01N2333/163 , G01N2333/8125 , G01N2333/96433 , G01N2333/966 , G01N2333/974 , C12Q2525/101
摘要： A method for identifying nucleic acid ligands to target molecules using the SELEX procedure wherein the candidate nucleic acids contain photoreactive groups and nucleic acid ligands identified thereby are claimed. The complexes of increased affinity nucleic acids and target molecules formed in the procedure are crosslinked by irradiation to facilitate separation from unbound nucleic acids. In other methods partitioning of high and low affinity nucleic acids is facilitated by primer extension steps as shown in the figure in which chain termination nucleotides, digestion resistant nucleotides or nucleotides that allow retention of the cDNA product on an affinity matrix are differentially incorporated into the cDNA products of either the high or low affinity nucleic acids and the cDNA products are treated accordingly to amplification, enzymatic or chemical digestion or by contact with an affinity matrix.
摘要翻译：使用SELEX方法鉴定靶分子的核酸配体的方法，其中候选核酸含有由其鉴定的光反应性基团和核酸配体。本方法中形成的增加的亲和核酸和靶分子的复合物通过照射进行交联，以促进与未结合核酸的分离。在其他方法中，通过如图所示的引物延伸步骤促进了高亲和性核酸的分配，其中使得能够将亲和基质上的cDNA产物保留的链终止核苷酸，消化抗性核苷酸或核苷酸差异并入cDNA 相应地对高亲和性核酸和cDNA产物的产物进行扩增，酶促或化学消化或通过与亲和基质接触。

4. 发明申请

US20030087301A1 Conditional-SELEX 有权
标题翻译：条件SELEX
公开(公告)号：US20030087301A1
公开(公告)日：2003-05-08
申请号：US10331083
申请日：2002-12-27
申请人： SomaLogic, Inc.
发明人： Jonathan Drew Smith , Larry Gold
IPC分类号： C12Q001/68 , C07H021/04 , C12P019/34
CPC分类号： C12N15/1048
摘要： The invention provides method for producing nucleic acid ligands that generate a signal, or cause a decrease in the level of a signal, in the presence of a target molecule or an environmental stimulus. The methods of the instant invention are collectively termed Conditional SELEX. The nucleic acid ligands of the instant invention are useful in any application where it is desirable to measure the concentration of a target molecule or detect and quantitate an environmental stimulus.
摘要翻译：本发明提供了在目标分子或环境刺激的存在下产生产生信号或引起信号水平降低的核酸配体的方法。本发明的方法被统称为条件SELEX。本发明的核酸配体可用于需要测量靶分子的浓度或检测和定量环境刺激的任何应用中。

5. 发明申请

US20020106652A1 Systematic evolution of ligands by exponential enrichment: photoselection of nucleic acid ligands and solution selex 失效
标题翻译：配体通过指数富集的系统演化：核酸配体的照片选择和溶液溶解
公开(公告)号：US20020106652A1
公开(公告)日：2002-08-08
申请号：US09882246
申请日：2001-06-14
申请人： SomaLogic, Inc.
发明人： Larry Gold , Michael Willis , Tad Koch , Steven Ringquist , Kirk Jensen , Brent Atkinson
IPC分类号： C12Q001/68 , C07H021/04 , C12P019/34 , C08K003/00
CPC分类号： G01N33/76 , A61K47/547 , A61K47/549 , B82Y5/00 , C07H19/06 , C07H19/10 , C07H21/00 , C07K14/001 , C12N9/1276 , C12N15/1048 , C12N15/115 , C12N2310/13 , C12N2310/322 , C12N2310/53 , C12Q1/37 , C12Q1/6811 , C12Q1/703 , C40B40/00 , F02B2075/027 , G01N33/531 , G01N33/532 , G01N33/535 , G01N33/56988 , G01N33/68 , G01N2333/163 , G01N2333/575 , G01N2333/62 , G01N2333/8125 , G01N2333/96433 , G01N2333/96455 , G01N2333/966 , G01N2333/9726 , G01N2333/974 , G01N2333/976 , C12Q2541/101 , C12Q2523/313
摘要： A method for identifying nucleic acid ligands to target molecules using the SELEX procedure. Nucleic acid candidate sequences contain photoreactive groups. After exposure of the nucleic acid sequences to the target molecule, nucleic acid-target molecule complexes are formed between nucleic acids having increased affinity to the target molecule and the target molecule. The complexes are irradiated such that photocrosslinks form between the photoreactive groups of the bound nucleic acids and the target molecule. The photocrosslinked complexes are separated from unbound nucleic acids, and the nucleic acids amplified to yield a ligand-enriched mixture of nucleic acids. Described herein are methods for improved partitioning between high and low affinity nucleic acid ligands identified through the SELEX method, termed solution SELEX. The solution SELEX method achieves partitioning between high and low affinity nucleic acid-target complexes through a number of methods, including (1) primer extension inhibition which results in differentiable cDNA products. Primer extension inhibition is achieved with the use of nucleic acid polymerases, including DNA or RNA polymerases, reverse transcriptase, and Qnull-replicase; (2) exonuclease hydrolysis inhibition which results in only the highest affinity ligands amplifying during PCR. This is achieved with the use of any 3nullnull5null double-stranded exonuclease; (3) linear to circle formation to generate molecules amplifiable during PCR; or (4) PCR amplification of single-stranded nucleic acids. A central theme of the method of the present invention is that the nucleic acid candidate mixture is screened in solution and results in preferential amplification of the highest affinity RNA ligand or catalytic RNA.
摘要翻译：使用SELEX方法鉴定靶向分子的核酸配体的方法。核酸候选序列含有光反应性基团。在将核酸序列暴露于靶分子后，在靶分子和靶分子之间具有增加的亲和力的核酸之间形成核酸 - 靶分子复合物。照射复合物，使得结合的核酸的光反应性基团与目标分子之间形成光交联。将光交联的复合物与未结合的核酸分离，并扩增核酸以产生富配体的核酸混合物。本文描述了通过SELEX方法鉴定的称为溶液SELEX的高亲和力核酸配体和低亲和力核酸配体之间的分配改进方法。解决方案SELEX方法通过多种方法实现高亲和力核酸 - 靶向复合物之间的分配，包括（1）导致差异化cDNA产物的引物延伸抑制。引物延伸抑制通过使用核酸聚合酶，包括DNA或RNA聚合酶，逆转录酶和Qbeta复制酶来实现; （2）外切核酸酶水解抑制，其导致PCR期间最高亲和力配体扩增。这是通过使用任何3' - > 5'双链外切核酸酶来实现的; （3）线性循环形成以产生PCR期间可扩增的分子; 或（4）单链核酸的PCR扩增。本发明方法的中心主题是将核酸候选混合物在溶液中筛选并导致最高亲和力RNA配体或催化RNA的优先扩增。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式