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1. 发明申请

US20160202259A1 APTAMERS SCREENING METHOD BASED ON GRAPHENE WITHOUT TARGET IMMOBILIZATION AND THE APTAMERS OBTAINED FROM THE METHOD 有权
公开(公告)号：US20160202259A1
公开(公告)日：2016-07-14
申请号：US15066514
申请日：2016-03-10
申请人： Korea University Research and Business Foundation
发明人： Man Bock Gu , Jee Woong Park , Tatavarty Rameshwar
IPC分类号： G01N33/569 , C12N15/115 , C12N7/00
CPC分类号： G01N33/57488 , B01D15/3823 , C12N7/00 , C12N9/1048 , C12N15/1048 , C12N15/115 , C12N2310/16 , C12N2310/351 , C12N2320/13 , C12N2330/30 , C12N2770/24351 , C12Q1/6811 , C12Y204/02012 , G01N33/5308 , G01N33/56983 , G01N33/57407 , G01N33/6893 , G01N2333/183 , G01N2333/91142 , C12Q2525/205 , C12Q2541/101
摘要： Provided is aptamers screening method based on graphene without target immobilization and the aptamers obtained from the method, and more particularly, a new GO-SELEX method without target immobilization in which a single-stranded nucleic acid pool may react with a non-bound target material or a counter-target material, after which a single-stranded nucleic acid which has not been bound to the target or counter-target may be separated by using the graphene. Also, the specific aptamer obtained through the above-described method may be used for diagnosing target related diseases.

2. 发明授权

US09322024B2 Aptamers screening method based on graphene without target immobilization and the aptamers obtained from the method 有权
标题翻译：基于没有靶固定的石墨烯的适体筛选方法和从该方法获得的适体
公开(公告)号：US09322024B2
公开(公告)日：2016-04-26
申请号：US14342241
申请日：2012-08-30
申请人： Man Bock Gu , Jee Woong Park , Tatavarty Rameshwar
发明人： Man Bock Gu , Jee Woong Park , Tatavarty Rameshwar
IPC分类号： C07H21/04 , C12N15/115 , C12N15/10 , C12Q1/68 , G01N33/569 , G01N33/574 , G01N33/68
CPC分类号： G01N33/57488 , B01D15/3823 , C12N7/00 , C12N9/1048 , C12N15/1048 , C12N15/115 , C12N2310/16 , C12N2310/351 , C12N2320/13 , C12N2330/30 , C12N2770/24351 , C12Q1/6811 , C12Y204/02012 , G01N33/5308 , G01N33/56983 , G01N33/57407 , G01N33/6893 , G01N2333/183 , G01N2333/91142 , C12Q2525/205 , C12Q2541/101
摘要： Provided is aptamers screening method based on graphene without target immobilization and the aptamers obtained from the method, and more particularly, a new GO-SELEX method without target immobilization in which a single-stranded nucleic acid pool may react with a non-bound target material or a counter-target material, after which a single-stranded nucleic acid which has not been bound to the target or counter-target may be separated by using the graphene. Also, the specific aptamer obtained through the above-described method may be used for diagnosing target related diseases.
摘要翻译：提供了基于没有靶固定的石墨烯的适体筛选方法和从该方法获得的适体，更具体地说，没有目标固定的新的GO-SELEX方法，其中单链核酸库可以与未结合的靶材料反应或反靶物质，之后可以通过使用石墨烯分离未结合靶或对靶的单链核酸。此外，通过上述方法获得的特异性适体可用于诊断目标相关疾病。

3. 发明授权

US08409795B2 Selex and photoSELEX 有权
标题翻译： Selex和photoSELEX
公开(公告)号：US08409795B2
公开(公告)日：2013-04-02
申请号：US12175388
申请日：2008-07-17
申请人： Daniel J. Schneider , Sheri K. Wilcox , Dominic Zichi , Dan Nieuwlandt , Jeff Carter , Larry Gold
发明人： Daniel J. Schneider , Sheri K. Wilcox , Dominic Zichi , Dan Nieuwlandt , Jeff Carter , Larry Gold
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6811 , C12N15/1048 , C12N15/111 , C12N15/115 , C12N2310/16 , C12N2320/13 , C12Q2541/101 , C12Q2525/205 , C12Q2525/117
摘要： The present disclosure describes improved SELEX methods for generating nucleic acid ligands that are capable of binding to target molecules and improved photoSELEX methods for generating photoreactive nucleic acid ligands that are capable of both binding and covalently crosslinking to target molecules. The disclosure further describes nucleic acid libraries having expanded physical and chemical properties and their use in SELEX and photoSELEX; methods for increasing the crosslinking efficiencies of photoaptamers; methods for producing photoaptamers having selective modifications that enhance functionality and minimize non-specific photoreactions; and methods for generating truncated nucleic acid ligands from nucleic acid ligands of longer length. The disclosure further describes aptamers and photoaptamers obtained by using any of the foregoing.
摘要翻译：本公开描述了用于产生能够结合靶分子的核酸配体的改进的SELEX方法和用于产生能够与靶分子结合和共价交联的光反应性核酸配体的改进的photoSELEX方法。本公开进一步描述了具有扩展的物理和化学性质及其在SELEX和photoSELEX中的用途的核酸文库; 提高光催化剂交联效率的方法; 制备具有增强功能并使非特异性光反应最小化的选择性修饰的光致抗体的方法; 以及用于从较长长度的核酸配体产生截短的核酸配体的方法。本公开进一步描述了通过使用上述任何一种获得的适体和光致抗体。

4. 发明授权

US08367329B2 Method of detecting interaction between nucleic acid and protein, and apparatus for the same 有权
标题翻译：检测核酸与蛋白质相互作用的方法及其设备
公开(公告)号：US08367329B2
公开(公告)日：2013-02-05
申请号：US12308370
申请日：2007-06-14
申请人： Kazunori Ikebukuro , Ryo Katayama , Eiji Takahashi , Hiroyuki Takamatsu
发明人： Kazunori Ikebukuro , Ryo Katayama , Eiji Takahashi , Hiroyuki Takamatsu
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6804 , G01N21/171 , C12Q2537/101 , C12Q2541/101
摘要： The invention is to easily detect an interaction between nucleic acid and protein with high sensitivity without the need of sample labeling with a fluorescent molecule or sample anchorage onto a metal thin-film. As means for it, the presence or absence of occurrence of the interaction between nucleic acid and protein in a sample (S) is detected in an optical manner. Specifically, the sample (S) is irradiated with excitation rays (Le) and with measuring rays (L2) for measuring a photothermal effect produced in the sample (S) through the irradiation with the excitation rays (Le). A measurement signal for the photothermal effect in the sample (S) by the excitation rays (Le) is produced on the basis of any phase change of the measuring rays (L2). A temporal variation in the measurement signal is used for making a judgment on the presence or absence of any occurrence of interaction between nucleic acid and protein.
摘要翻译：本发明可以容易地以高灵敏度检测核酸和蛋白质之间的相互作用，而不需要用荧光分子或样品锚定物将样品标记在金属薄膜上。作为其手段，以光学方式检测样品（S）中核酸和蛋白质之间的相互作用的存在或不存在。具体地，用激发光线（Le）和用于通过激发光线（Le）的照射测量样品（S）中产生的光热效应的测量光线（L2）来照射样品（S）。基于测量光线（L2）的任何相变，产生通过激发光线（Le）在样品（S）中的光热效应的测量信号。使用测量信号的时间变化来判断核酸和蛋白质之间是否存在任何相互作用的发生。

5. 发明申请

US20120225494A1 DNA LIGANDS FOR AFLATOXIN AND ZEARALENONE 审中-公开
标题翻译：用于AFLATOXIN和ZEARALENONE的DNA配体
公开(公告)号：US20120225494A1
公开(公告)日：2012-09-06
申请号：US13391426
申请日：2010-08-20
申请人： Linda Chryseis Le , Jorge Andres Cruz-Aguado , Gregory Allen Penner
发明人： Linda Chryseis Le , Jorge Andres Cruz-Aguado , Gregory Allen Penner
IPC分类号： G01N33/566 , C07H21/04 , G01N30/02 , G01N21/64 , G01N27/62
CPC分类号： G01N33/56961 , C12N15/1048 , C12N15/115 , C12N2310/16 , C12Q1/6811 , C12Q2563/107 , C12Q2541/101 , C12Q2525/205
摘要： The present invention relates to DNA ligands capable of binding to aflatoxin and zearalenone. The invention relates also to methods for determining the presence and concentration of aflatoxin and zearalenone in samples such as agricultural and food products, and to methods for removing or reducing the level of aflatoxin and zearalenone in samples such as agricultural and food products. The invention further relates to methods for identifying DNA ligands capable of binding to aflatoxin and zearalenone. The invention further relates to new DNA sequences.
摘要翻译：本发明涉及能够结合黄曲霉毒素和玉米赤霉烯酮的DNA配体。本发明还涉及确定农业和食品等样品中黄曲霉毒素和玉米赤霉烯酮的存在和浓度的方法，以及用于去除或降低样品（例如农业和食品）中的黄曲霉毒素和玉米赤霉烯酮水平的方法。本发明还涉及鉴定能够结合黄曲霉毒素和玉米赤霉烯酮的DNA配体的方法。本发明还涉及新的DNA序列。

6. 发明授权

US08071737B2 Nucleic acid ligand complexes 有权
标题翻译：核酸配体配合物
公开(公告)号：US08071737B2
公开(公告)日：2011-12-06
申请号：US12624314
申请日：2009-11-23
申请人： Larry Gold , Paul Schmidt , Nebojsa Janjic
发明人： Larry Gold , Paul Schmidt , Nebojsa Janjic
IPC分类号： C07H21/02 , A61B5/055
CPC分类号： A61K9/1271 , A61K9/1272 , C07H19/06 , C07H19/10 , C07H21/00 , C07K14/001 , C12N9/1276 , C12N15/1048 , C12N15/115 , C12N2310/13 , C12N2310/322 , C12N2310/53 , C12Q1/37 , C12Q1/6811 , C40B40/00 , F02B2075/027 , G01N33/531 , G01N33/532 , G01N33/535 , G01N33/56988 , G01N33/68 , G01N33/76 , G01N2333/163 , G01N2333/575 , G01N2333/62 , G01N2333/8125 , G01N2333/96433 , G01N2333/96455 , G01N2333/966 , G01N2333/9726 , G01N2333/974 , G01N2333/976 , C12Q2541/101 , C12Q2563/161
摘要： This invention discloses a method for preparing a therapeutic or diagnostic complex comprised of a nucleic acid ligand and a lipophilic compound or non-immunogenic, high molecular weight compound by identifying a nucleic acid ligand by SELEX methodology and associating the nucleic acid ligand with a lipophilic compound or a non-immunogenic, high molecular weight compound. The invention further discloses complexes comprising one or more nucleic acid ligands in association with a lipophilic compound or non-immunogenic, high molecular weight compound.
摘要翻译：本发明公开了一种由核酸配体和亲脂性化合物或非免疫原性高分子量化合物组成的治疗或诊断复合物的方法，其通过使用SELEX方法鉴定核酸配体并将核酸配体与亲脂性化合物或非免疫原性的高分子量化合物。本发明进一步公开了包含与亲脂性化合物或非免疫原性高分子量化合物结合的一种或多种核酸配体的复合物。

7. 发明申请

US20110059555A1 BRANCHED AND MULTI-CHAIN NUCLEIC ACID SWITCHES FOR SENSING AND SCREENING 有权
标题翻译：用于感测和筛选的分支和多链式核酸开关
公开(公告)号：US20110059555A1
公开(公告)日：2011-03-10
申请号：US12901762
申请日：2010-10-11
申请人： Philip N. Borer , Bruce S. Hudson
发明人： Philip N. Borer , Bruce S. Hudson
IPC分类号： G01N33/53 , C07H21/04 , C07H21/02
CPC分类号： C12N15/115 , C12N2310/16 , C12N2310/3517 , C12Q1/6818 , C12Q1/70 , C12Q1/701 , Y02A50/52 , Y02A50/58 , C12Q2541/101 , C12Q2525/313 , C12Q2525/205 , C12Q2525/301 , C12Q2565/301
摘要： Embodiments of the invention relate to a branched or multichain nucleic acid switch adapted to switch from a first conformation to a second conformation upon ligand binding. The switch includes a probe strand, P, which includes the ligand binding domain; a switching framework which includes a cover strand (C), and a tether that holds P and C together and a signaling apparatus. Some embodiments include a toggle strand (T) where now the tether holds P, C, T, and the signaling apparatus together. As the switch changes between the first and second conformations; the signaling apparatus reports the state of the switch. The signaling entity is typically a lumiphore and a quencher located along the switching framework. Nucleic acid switches have applications in real time assays for diverse agents including infectious agents, environmental toxins, and terrorist agents, as well as screening methods for such agents. Further applications are found for nanoelectronics, nanofabrication and nanomachines.
摘要翻译：本发明的实施方案涉及一种支链或多链核酸开关，其适于在配体结合时从第一构象切换到第二构象。该开关包括探针链，其包括配体结合结构域; 包括盖链（C）和将P和C保持在一起的系链和信号装置的切换框架。一些实施例包括切换链（T），其中系绳将P，C，T和信令装置保持在一起。当开关在第一和第二构象之间变化时; 信令装置报告交换机的状态。信令实体通常是沿着交换框架定位的集线器和猝灭器。核酸开关具有用于多种试剂（包括感染因子，环境毒素和恐怖分子）的实时分析的应用，以及这些试剂的筛选方法。发现纳米电子学，纳米制造和纳米机械的其他应用。

8. 发明申请

US20100304991A1 METHOD OF SELECTING APTAMERS 有权
标题翻译：选择APTAMERS的方法
公开(公告)号：US20100304991A1
公开(公告)日：2010-12-02
申请号：US12739087
申请日：2008-10-22
申请人： Clive Gavin Brown
发明人： Clive Gavin Brown
IPC分类号： C40B30/04
CPC分类号： C12N15/1048 , C12Q1/6811 , C12Q2541/101
摘要： The present invention is a method for the identification of one or more aptamers to at least one target molecule, the method comprising: selecting candidate aptamer sequences that bind to a target molecule, assigning to the bound sequences a measure (fitness function) of each sequence's aptameric potential, allowing evolution of some or all of the sequences to create a new mixture of candidate sequences, and repeating the method with the newly created candidate aptamer pool until the aggregate aptameric potential of the candidate pool reaches a plateau, wherein sequences present in the final pool are optimal aptamers to the target molecule.
摘要翻译：本发明是用于鉴定至少一种靶分子的一种或多种适体的方法，所述方法包括：选择与靶分子结合的候选适体序列，向所述结合序列分配每个序列的测量（适应度函数）允许进行一些或所有序列以产生候选序列的新混合物，并且用新产生的候选适体池重复该方法，直到候选池的总体适体潜力达到平台，其中存在于最终池是靶分子的最佳适体。

9. 发明申请

US20100167280A1 OLIGONUCLEOTIDES FOR DISCRIMINATING RELATED NUCLEIC ACID SEQUENCES 审中-公开
标题翻译：用于鉴别相关核酸序列的寡核苷酸
公开(公告)号：US20100167280A1
公开(公告)日：2010-07-01
申请号：US12377044
申请日：2007-08-10
申请人： Ivan Brukner , Damian Labuda , Maja Krajinovic
发明人： Ivan Brukner , Damian Labuda , Maja Krajinovic
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6811 , C12N2710/20011 , C12Q1/708 , C12Q2541/101 , C12Q2537/143 , C12Q2525/179
摘要： An in vitro selection method is described which identifies oligonucleotide probes that discriminate amongst closely related nucleic acid sequences and which involves iterative hybridizations, including subtractive hybridization. Using the method, oligonucleotides are identified which can discriminate among human papilloma virus (HPV) subtypes. Corresponding methods and kits for the detection of nucleic acids are described, which methods and kits may be used in analytical, diagnostic, research and related applications.
摘要翻译：描述了鉴定区分密切相关的核酸序列并涉及迭代杂交的寡核苷酸探针的体外选择方法，包括消减杂交。使用该方法鉴定出能区分人乳头状瘤病毒（HPV）亚型的寡核苷酸。描述了用于检测核酸的相应方法和试剂盒，哪些方法和试剂盒可用于分析，诊断，研究和相关应用。

10. 发明申请

US20090130672A1 METHODS FOR IDENTIFYING NOVEL PESTICIDAL GENE HOMOLOGUES 审中-公开
公开(公告)号：US20090130672A1
公开(公告)日：2009-05-21
申请号：US11937865
申请日：2007-11-09
申请人： Andre R. Abad , Ronald D. Flannagan , Bin Hu , Cao Guo Yu
发明人： Andre R. Abad , Ronald D. Flannagan , Bin Hu , Cao Guo Yu
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6809 , C12Q1/689 , C12Q2541/101
摘要： Methods and compositions for identifying novel pesticidal gene homologues are provided. Specifically, the methods of the invention comprise systematically designing oligonucleotide primers that are specific for a pesticidal gene of interest and performing successive rounds of PCR amplification of nucleic acid material from a microorganism, particularly a Bacillus thuringiensis strain, to identify novel homologues of known pesticidal genes. Oligonucleotide primers that can be used to practice the present methods are further disclosed.

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