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1. 发明授权

US07452666B2 Synthesis of DNA 失效
标题翻译： DNA的合成
公开(公告)号：US07452666B2
公开(公告)日：2008-11-18
申请号：US10394911
申请日：2003-03-21
申请人： Raymond P. Mariella, Jr.
发明人： Raymond P. Mariella, Jr.
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12N15/1031 , C07H21/00 , C12N15/1027 , C12Q1/686 , C12Q2533/107 , C12Q2521/101
摘要： A method of synthesizing a desired double-stranded DNA of a predetermined length and of a predetermined sequence. Preselected sequence segments that will complete the desired double-stranded DNA are determined. Preselected segment sequences of DNA that will be used to complete the desired double-stranded DNA are provided. The preselected segment sequences of DNA are assembled to produce the desired double-stranded DNA.
摘要翻译：合成预定长度和预定序列的所需双链DNA的方法。确定将完成所需双链DNA的预选序列片段。提供将用于完成所需双链DNA的预选择的DNA序列。组装DNA的预选区段序列以产生所需的双链DNA。

2. 发明授权

US5589136A Silicon-based sleeve devices for chemical reactions 失效
标题翻译：用于化学反应的硅基套管装置
公开(公告)号：US5589136A
公开(公告)日：1996-12-31
申请号：US492678
申请日：1995-06-20
申请人： M. Allen Northrup , Raymond P. Mariella, Jr. , Anthony V. Carrano , Joseph W. Balch
发明人： M. Allen Northrup , Raymond P. Mariella, Jr. , Anthony V. Carrano , Joseph W. Balch
IPC分类号： C12N15/09 , B01J19/00 , B01L7/00 , B81B1/00 , B81C3/00 , C12M1/00 , C12Q1/68 , G01N21/03 , G01N21/05 , G01N21/69 , G01N21/76 , B01L3/00
CPC分类号： G01N21/69 , B01J19/0046 , B01J19/0093 , B01L7/52 , G01N21/0332 , G01N21/76 , B01J2219/00828 , B01J2219/00853 , B01J2219/00873 , B01J2219/00889 , B01J2219/00891 , B01J2219/00912 , B01J2219/00936 , B01J2219/00945 , B01J2219/00952 , B81C3/00 , G01N2021/0346 , G01N21/05
摘要： A silicon-based sleeve type chemical reaction chamber that combines heaters, such as doped polysilicon for heating, and bulk silicon for convection cooling. The reaction chamber combines a critical ratio of silicon and silicon nitride to the volume of material to be heated (e.g., a liquid) in order to provide uniform heating, yet low power requirements. The reaction chamber will also allow the introduction of a secondary tube (e.g., plastic) into the reaction sleeve that contains the reaction mixture thereby alleviating any potential materials incompatibility issues. The reaction chamber may be utilized in any chemical reaction system for synthesis or processing of organic, inorganic, or biochemical reactions, such as the polymerase chain reaction (PCR) and/or other DNA reactions, such as the ligase chain reaction, which are examples of a synthetic, thermal-cycling-based reaction. The reaction chamber may also be used in synthesis instruments, particularly those for DNA amplification and synthesis.
摘要翻译：一种硅基套管型化学反应室，其结合加热器，例如用于加热的掺杂多晶硅和用于对流冷却的体硅。反应室将硅和氮化硅的临界比与待加热材料（例如液体）的体积相结合，以便提供均匀的加热，但低功率要求。反应室还将允许将二次管（例如塑料）引入到包含反应混合物的反应套筒中，从而减轻任何潜在的材料不相容性问题。反应室可用于任何用于合成或加工有机，无机或生物化学反应的化学反应体系，例如聚合酶链式反应（PCR）和/或其它DNA反应，例如连接酶链式反应，其为实施例的合成，热循环反应。反应室也可用于合成仪器，特别是用于DNA扩增和合成的仪器。

3. 发明授权

US09052255B2 System for autonomous monitoring of bioagents 有权
标题翻译：生物制剂自主监测系统
公开(公告)号：US09052255B2
公开(公告)日：2015-06-09
申请号：US12786248
申请日：2010-05-24
申请人： Richard G. Langlois , Fred P. Milanovich , Billy W. Colston, Jr. , Steve B. Brown , Don A. Masquelier , Raymond P. Mariella, Jr. , Kodomudi Venkateswaran
发明人： Richard G. Langlois , Fred P. Milanovich , Billy W. Colston, Jr. , Steve B. Brown , Don A. Masquelier , Raymond P. Mariella, Jr. , Kodomudi Venkateswaran
IPC分类号： G01N33/543 , G01N30/96 , G01N33/00 , G01N1/22 , G01N35/08 , G01N15/14
CPC分类号： G01N1/2202 , G01N1/2211 , G01N15/1459 , G01N35/08 , G01N2001/2217 , G06F19/00 , Y02A90/26
摘要： An autonomous monitoring system for monitoring for bioagents. A collector gathers the air, water, soil, or substance being monitored. A sample preparation means for preparing a sample is operatively connected to the collector. A detector for detecting the bioagents in the sample is operatively connected to the sample preparation means. One embodiment of the present invention includes confirmation means for confirming the bioagents in the sample.
摘要翻译：一个用于监测生物标签的自主监测系统。收集者收集被监测的空气，水，土壤或物质。用于制备样品的样品制备装置可操作地连接到收集器。用于检测样品中的生物试剂的检测器可操作地连接到样品制备装置。本发明的一个实施方案包括用于确认样品中的生物试剂的确认装置。

4. 发明授权

US08727129B2 Microfluidic ultrasonic particle separators with engineered node locations and geometries 有权
标题翻译：具有工程节点位置和几何形状的微流体超声波颗粒分离器
公开(公告)号：US08727129B2
公开(公告)日：2014-05-20
申请号：US13571640
申请日：2012-08-10
申请人： Klint A. Rose , Karl A. Fisher , Douglas A. Wajda , Raymond P. Mariella, Jr. , Christopher Bailey , Dietrich Dehlinger , Maxim Shusteff , Byoungsok Jung , Kevin D. Ness
发明人： Klint A. Rose , Karl A. Fisher , Douglas A. Wajda , Raymond P. Mariella, Jr. , Christopher Bailey , Dietrich Dehlinger , Maxim Shusteff , Byoungsok Jung , Kevin D. Ness
IPC分类号： B03B5/00
CPC分类号： B01L3/50273 , B01D21/28 , B01D21/283 , B01D2221/10 , B01L3/502761 , B01L2200/0631 , B01L2200/0636 , B01L2200/0652 , B01L2300/0816 , B01L2300/0864 , B01L2400/0439 , B01L2400/0487 , G01N1/4077 , G01N33/48728 , G01N33/491 , G01N2001/4094
摘要： An ultrasonic microfluidic system includes a separation channel for conveying a sample fluid containing small particles and large particles, flowing substantially parallel, adjacent to a recovery fluid, with which it is in contact. An acoustic transducer produces an ultrasound standing wave, that generates a pressure field having at least one node of minimum pressure amplitude. An acoustic extension structure is located proximate to said separation channel for positioning said acoustic node off center in said acoustic area and concentrating the large particles in said recovery fluid stream.
摘要翻译：超声波微流体系统包括用于输送包含小颗粒和大颗粒的样品流体的分离通道，所述样品流体基本上平行于与其接触的回收流体相邻流动。声换能器产生超声驻波，其产生具有至少一个最小压力振幅节点的压力场。声学延伸结构位于所述分离通道附近，用于将所述声音节点偏离所述声学区域的中心，并将大颗粒集中在所述回收流体流中。

5. 发明授权

US06761811B2 Multi-stage separations based on dielectrophoresis 失效
标题翻译：基于介电电泳的多级分离
公开(公告)号：US06761811B2
公开(公告)日：2004-07-13
申请号：US09819108
申请日：2001-03-27
申请人： Raymond P. Mariella, Jr.
发明人： Raymond P. Mariella, Jr.
IPC分类号： G01N27447
CPC分类号： B03C5/026
摘要： A system utilizing multi-stage traps based on dielectrophoresis. Traps with electrodes arranged transverse to the flow and traps with electrodes arranged parallel to the flow with combinations of direct current and alternating voltage are used to trap, concentrate, separate, and/or purify target particles.
摘要翻译：基于介电电泳的多级阱的系统。使用具有横向于流动的电极的陷阱和具有与直流电流和交流电压的组合平行布置的电极的陷阱，以捕获，浓缩，分离和/或净化靶颗粒。

6. 发明授权

US08524064B2 Three dimensional microelectrode system for dielectrophoresis 有权
标题翻译：用于介电电泳的三维微电极系统
公开(公告)号：US08524064B2
公开(公告)日：2013-09-03
申请号：US12843269
申请日：2010-07-26
申请人： Dietrich A. Dehlinger , Klint A. Rose , Maxim Shusteff , Christopher G. Bailey , Raymond P. Mariella, Jr.
发明人： Dietrich A. Dehlinger , Klint A. Rose , Maxim Shusteff , Christopher G. Bailey , Raymond P. Mariella, Jr.
IPC分类号： G01N27/26 , G01N27/453 , B03C5/02 , B01D57/02
CPC分类号： B03C5/005 , B01L2400/0424 , B03C5/026 , G01N27/447
摘要： A dielectrophoresis apparatus for separating particles from a sample, including an apparatus body; a dielectrophoresis channel in the apparatus body, the dielectrophoresis channel having a central axis, a bottom, a top, a first side, and a second side; a first mesa projecting into the dielectrophoresis channel from the bottom and extending from the first side across the dielectrophoresis channel to the second side, the first mesa extending at an angle to the central axis of the dielectrophoresis channel; a first electrode extending along the first mesa; a second mesa projecting into the dielectrophoresis channel from the bottom and extending from the first side across the dielectrophoresis channel to the second side, the second mesa extending at an angle to the central axis of the dielectrophoresis channel; a space between at least one of the first electrode and the second side or the second electrode and the second side; and a gap between the first electrode and the second electrode.
摘要翻译：一种用于从样品中分离颗粒的介电电泳装置，包括装置体; 所述设备主体中的介电电泳通道，所述介电电泳通道具有中心轴线，底部，顶部，第一侧面和第二侧面; 第一台面从底部突出到介电电泳通道中并且从第一侧穿过介电电泳通道延伸到第二侧，第一台面以与介电电泳通道的中心轴线成一定角度延伸; 沿第一台面延伸的第一电极; 第二台面从底部突出到介电电泳通道中，并从第一侧穿过介电电泳通道延伸到第二侧，第二台面以与介电电泳通道的中心轴成一定角度延伸; 第一电极和第二电极和第二电极和第二侧中的至少一个之间的空间; 以及第一电极和第二电极之间的间隙。

7. 发明授权

US08470537B2 Sequential addition of short DNA oligos in DNA-polymerase-based synthesis reactions 有权
标题翻译：在基于DNA聚合酶的合成反应中连续添加短DNA寡核苷酸
公开(公告)号：US08470537B2
公开(公告)日：2013-06-25
申请号：US12959789
申请日：2010-12-03
申请人： Shea N. Gardner , Raymond P. Mariella, Jr. , Allen T. Christian , Jennifer A. Young , David S. Clague
发明人： Shea N. Gardner , Raymond P. Mariella, Jr. , Allen T. Christian , Jennifer A. Young , David S. Clague
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6844 , C07H21/00 , C12N15/10 , C12N15/1027 , C12N15/1031 , C12Q1/6813 , C12Q1/686 , C12Q2521/101 , C12Q2533/107 , C12Q2525/143
摘要： A method of preselecting a multiplicity of DNA sequence segments that will comprise the DNA molecule of user-defined sequence, separating the DNA sequence segments temporally, and combining the multiplicity of DNA sequence segments with at least one polymerase enzyme wherein the multiplicity of DNA sequence segments join to produce the DNA molecule of user-defined sequence. Sequence segments may be of length n, where n is an odd integer. In one embodiment the length of desired hybridizing overlap is specified by the user and the sequences and the protocol for combining them are guided by computational (bioinformatics) predictions. In one embodiment sequence segments are combined from multiple reading frames to span the same region of a sequence, so that multiple desired hybridizations may occur with different overlap lengths.
摘要翻译：预选选择将包含用户定义序列的DNA分子的多个DNA序列片段的方法，将DNA序列片段在时间上分离，并将多个DNA序列片段与至少一种聚合酶结合，其中多个DNA序列片段加入生产用户定义序列的DNA分子。序列段可以是长度为n，其中n是奇整数。在一个实施例中，期望的杂交重叠的长度由用户指定，并且用于组合它们的序列和协议由计算（生物信息学）预测指导。在一个实施例中，序列片段从多个读取框架组合以跨越序列的相同区域，使得可以以不同的重叠长度发生多个期望的杂交。

8. 发明授权

US6154276A Waveguide detection of right-angle-scattered light in flow cytometry 失效
标题翻译：流式细胞术中直角散射光的波导检测
公开(公告)号：US6154276A
公开(公告)日：2000-11-28
申请号：US27764
申请日：1998-02-23
申请人： Raymond P. Mariella, Jr.
发明人： Raymond P. Mariella, Jr.
IPC分类号： G01N15/14 , G01N21/00
CPC分类号： G01N15/1436
摘要： A transparent flow cell is used as an index-guided optical waveguide. A detector for the flow cell but not the liquid stream detects the Right-Angle-Scattered (RAS) Light exiting from one end of the flow cell. The detector(s) could view the trapped RAS light from the flow cell either directly or through intermediate optical light guides. If the light exits one end of the flow cell, then the other end of the flow cell can be given a high-reflectivity coating to approximately double the amount of light collected. This system is more robust in its alignment than the traditional flow cytometry systems which use imaging optics, such as microscope objectives.
摘要翻译：使用透明流通池作为折射引导光波导。用于流动池而不是液体流的检测器检测从流动池的一端排出的直角散射（RAS）光。检测器可以直接或通过中间光学光导来观察来自流动池的被捕获的RAS光。如果光离开流动池的一端，则流动池的另一端可以被给予高反射率涂层，使其收集的光量大约增加一倍。该系统在比对比使用成像光学器件的传统流式细胞仪系统如显微镜目标更加坚固。

9. 发明授权

US5404026A Infrared-sensitive photocathode 失效
标题翻译：红外敏感光电阴极
公开(公告)号：US5404026A
公开(公告)日：1995-04-04
申请号：US004766
申请日：1993-01-14
申请人： Raymond P. Mariella, Jr. , Gregory A. Cooper
发明人： Raymond P. Mariella, Jr. , Gregory A. Cooper
IPC分类号： H01J1/34 , H01L29/161 , H01L29/205
CPC分类号： H01J1/34 , H01J2201/3423
摘要： A single-crystal, multi-layer device incorporating an IR absorbing layer that is compositionally different from the Ga.sub.x Al.sub.1-x Sb layer which acts as the electron emitter. Many different IR absorbing layers can be envisioned for use in this embodiment, limited only by the ability to grow quality material on a chosen substrate. A non-exclusive list of possible IR absorbing layers would include GaSb, InAs and InAs/Ga.sub.w In.sub.y Al.sub.1-y-w Sb superlattices. The absorption of the IR photon excites an electron into the conduction band of the IR absorber. An externally applied electric field then transports electrons from the conduction band of the absorber into the conduction band of the Ga.sub.x Al.sub.1-x Sb, from which they are ejected into vacuum. Because the band alignments of Ga.sub.x Al.sub.1-x Sb can be made the same as that of GaAs, emitting efficiencies comparable to GaAs photocathodes are obtainable. The present invention provides a photocathode that is responsive to wavelengths within the range of 0.9 .mu.m to at least 10 .mu.m.
摘要翻译：结合了IR吸收层的单晶多层器件，该吸收层在组成上不同于充当电子发射体的GaxAl1-xSb层。可以设想许多不同的IR吸收层用于该实施方案中，这仅限于在所选择的基底上生长优质材料的能力。可能的IR吸收层的非排他性列表将包括GaSb，InAs和InAs / GawInyAl1-y-wSb超晶格。 IR光子的吸收激发电子进入IR吸收体的导带。外部施加的电场然后将电子从吸收器的导带传输到GaxAl1-xSb的导带，从它们被喷射到真空中。因为GaxAl1-xSb的带对准可以与GaAs相同，所以可获得与GaAs光电阴极相当的发射效率。本发明提供一种响应波长在0.9μm至至少10μm之间的光电阴极。

10. 发明授权

US09480935B2 Systems and methods for separating particles and/or substances from a sample fluid 有权
标题翻译：用于从样品流体中分离颗粒和/或物质的系统和方法
公开(公告)号：US09480935B2
公开(公告)日：2016-11-01
申请号：US12024762
申请日：2008-02-01
申请人： Raymond P. Mariella, Jr. , George M. Dougherty , John M. Dzenitis , Robin R. Miles , David S. Clague
发明人： Raymond P. Mariella, Jr. , George M. Dougherty , John M. Dzenitis , Robin R. Miles , David S. Clague
IPC分类号： B01D17/06 , G01N15/02 , G01N1/40 , G01N15/14
CPC分类号： B01D17/06 , G01N1/4077 , G01N2001/4072 , G01N2015/0288
摘要： Systems and methods for separating particles and/or toxins from a sample fluid. A method according to one embodiment comprises simultaneously passing a sample fluid and a buffer fluid through a chamber such that a fluidic interface is formed between the sample fluid and the buffer fluid as the fluids pass through the chamber, the sample fluid having particles of interest therein; applying a force to the fluids for urging the particles of interest to pass through the interface into the buffer fluid; and substantially separating the buffer fluid from the sample fluid.
摘要翻译：用于从样品流体中分离颗粒和/或毒素的系统和方法。根据一个实施方案的方法包括同时使样品流体和缓冲流体通过室，使得当流体通过室时在样品流体和缓冲流体之间形成流体界面，样品流体具有目标颗粒 ; 向流体施加力以促使感兴趣的颗粒通过界面进入缓冲流体; 并且从样品流体基本上分离缓冲流体。

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