专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20190241921A1 METHODS FOR IN VITRO JOINING AND COMBINATORIAL ASSEMBLY OF NUCLEIC ACID MOLECULES 审中-公开
公开(公告)号：US20190241921A1
公开(公告)日：2019-08-08
申请号：US16388722
申请日：2019-04-18
申请人： Synthetic Genomics, Inc.
发明人： Daniel G. Gibson , Hamilton O. Smith , Clyde A. Hutchison , Lei Young , J. Craig Venter
IPC分类号： C12P19/34 , C12N15/66 , C12N15/10 , C12N15/64
CPC分类号： C12P19/34 , C12N15/10 , C12N15/1027 , C12N15/64 , C12N15/66
摘要： The present invention relates to methods of joining two or more double-stranded (ds) or single-stranded (ss) DNA molecules of interest in vitro, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule of each pair share a region of sequence identity. The method allows the joining of a large number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes. It can be used, e.g., to join synthetically produced sub-fragments of a gene or genome of interest. Kits for performing the method are also disclosed. The methods of joining DNA molecules may be used to generate combinatorial libraries useful to generate, for example, optimal protein expression through codon optimization, gene optimization, and pathway optimization.

2. 发明申请

US20180353621A1 GENETIC CONSTRUCT 审中-公开
公开(公告)号：US20180353621A1
公开(公告)日：2018-12-13
申请号：US15762089
申请日：2016-10-25
申请人： Quethera Limited
发明人： Peter WIDDOWSON , Keith MARTIN
IPC分类号： A61K48/00 , C07K14/48 , C12N15/85 , C12N15/10 , C12N15/62 , A61K38/18
CPC分类号： A61K48/0066 , A61K38/00 , A61K38/185 , A61K48/0058 , C07K14/48 , C12N15/1027 , C12N15/62 , C12N15/85 , C12N15/86 , C12N15/8645 , C12N2830/008 , C12N2840/203
摘要： The invention provides genetic constructs and recombinant vectors comprising such constructs. The constructs and vectors can be used in gene therapy methods for treating a range of disorders, including glaucoma and deafness, or for promoting nerve regeneration and/or survival.

3. 发明申请

US20180273936A1 VARIANT LIBRARIES OF THE IMMUNOLOGICAL SYNAPSE AND SYNTHESIS THEREOF 审中-公开
公开(公告)号：US20180273936A1
公开(公告)日：2018-09-27
申请号：US15921537
申请日：2018-03-14
申请人： TWIST BIOSCIENCE CORPORATION
发明人： Anthony COX , Siyuan CHEN
IPC分类号： C12N15/10 , C12Q1/6806
CPC分类号： C12N15/1093 , C12N15/1027 , C12Q1/6806
摘要： Disclosed herein are methods for the generation of highly accurate nucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The nucleic acid sequence may encode for all or part of a reference domain of a CAR. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a non-saturating library of variants. The variant nucleic acid libraries described herein may be designed for further processing by transcription or translation. The variant nucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of a disease, such as cancer.

4. 发明申请

US20180002707A1 METHODS AND MATERIALS FOR ASSEMBLING NUCLEIC ACID CONSTRUCTS 审中-公开
公开(公告)号：US20180002707A1
公开(公告)日：2018-01-04
申请号：US15544752
申请日：2016-01-20
申请人： MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCH
发明人： Stephen C. Ekker , Karl J. Clark , Jarryd M. Campbell , Chun Hang Alvin Ma
IPC分类号： C12N15/66 , C12N9/22
CPC分类号： C12N15/66 , C07K2319/00 , C07K2319/80 , C12N9/22 , C12N15/102 , C12N15/1027 , C12N15/1031 , C12N15/64
摘要： This document provides methods and materials for assembling nucleic acid constructs (e.g., TALENs). For example, methods for assembling TALEs that are rapid, flexible for use in many cloning scaffolds (such as common nuclease and nickase backbones), and achievable with standard molecular biology laboratory tools, thereby making TALEs a more accessible genome system, are provided.

5. 发明授权

US09562250B2 Methods for nucleic acid manipulation 有权
标题翻译：核酸操作方法
公开(公告)号：US09562250B2
公开(公告)日：2017-02-07
申请号：US14313193
申请日：2014-06-24
申请人： THE PENN STATE RESEARCH FOUNDATION
发明人： Stephen J Benkovic , Frank Salinas
IPC分类号： C12Q1/68 , C12P19/34 , C12N15/10 , C07H21/02
CPC分类号： C12P19/34 , C12N15/1027 , C12Q1/6806 , C12Q1/6844 , C12Q1/686 , C12Q2521/507 , C12Q2521/513 , C12Q2527/125
摘要： A method for replicating and amplifying a target nucleic acid sequence is described. A method of the invention involves the formation of a recombination intermediate without the prior denaturing of a nucleic acid duplex through the use of a recombination factor. The recombination intermediate is treated with a high fidelity polymerase to permit the replication and amplification of the target nucleic acid sequence. In preferred embodiments, the polymerase comprises a polymerase holoenzyme. In further preferred embodiments, the recombination factor is bacteriophage T4 UvsX protein or homologs from other species, and the polymerase holoenzyme comprises a polymerase enzyme, a clamp protein and a clamp loader protein, derived from viral, bacteriophage, prokaryotic, archaebacterial, or eukaryotic systems.
摘要翻译：描述了用于复制和扩增靶核酸序列的方法。本发明的方法涉及通过使用重组因子而不具有核酸双链体的先前变性而形成重组中间体。用高保真聚合酶处理重组中间体以允许靶核酸序列的复制和扩增。在优选的实施方案中，聚合酶包含聚合酶全酶。在进一步优选的实施方案中，重组因子是噬菌体T4 UvsX蛋白或来自其他物种的同源物，聚合酶全酶包含衍生自病毒，噬菌体，原核，古细菌或真核系统的聚合酶，钳夹蛋白和夹紧装载蛋白。

6. 发明申请

US20160237475A1 Methods For Nucleic Acid Manipulation 有权
标题翻译：核酸操作方法
公开(公告)号：US20160237475A1
公开(公告)日：2016-08-18
申请号：US15070529
申请日：2016-03-15
申请人： THE PENN STATE RESEARCH FOUNDATION
发明人： Stephen J BENKOVIC , Frank Salinas
IPC分类号： C12Q1/68
CPC分类号： C12P19/34 , C12N15/1027 , C12Q1/6806 , C12Q1/6844 , C12Q1/686 , C12Q2521/507 , C12Q2521/513 , C12Q2527/125
摘要： A method for replicating and amplifying a target nucleic acid sequence is described. A method of the invention involves the formation of a recombination intermediate without the prior denaturing of a nucleic acid duplex through the use of a recombination factor. The recombination intermediate is treated with a high fidelity polymerase to permit the replication and amplification of the target nucleic acid sequence. In preferred embodiments, the polymerase comprises a polymerase holoenzyme. In further preferred embodiments, the recombination factor is bacteriophage T4 UvsX protein or homologs from other species, and the polymerase holoenzyme comprises a polymerase enzyme, a clamp protein and a clamp loader protein, derived from viral, bacteriophage, prokaryotic, archaebacterial, or eukaryotic systems.
摘要翻译：描述了用于复制和扩增靶核酸序列的方法。本发明的方法涉及通过使用重组因子而不具有核酸双链体的先前变性而形成重组中间体。用高保真聚合酶处理重组中间体以允许靶核酸序列的复制和扩增。在优选的实施方案中，聚合酶包含聚合酶全酶。在进一步优选的实施方案中，重组因子是噬菌体T4 UvsX蛋白或来自其他物种的同源物，聚合酶全酶包含衍生自病毒，噬菌体，原核，古细菌或真核系统的聚合酶，钳夹蛋白和夹紧装载蛋白。

7. 发明授权

US09340826B2 Method of preparing nucleic acid molecules 有权
标题翻译：制备核酸分子的方法
公开(公告)号：US09340826B2
公开(公告)日：2016-05-17
申请号：US14235799
申请日：2012-08-01
申请人： Duhee Bang , Hwangbeom Kim , Hyojun Han
发明人： Duhee Bang , Hwangbeom Kim , Hyojun Han
IPC分类号： C12Q1/68 , C12N15/10
CPC分类号： C12N15/1093 , C12N15/1027 , C12N15/1031 , C12N15/1065 , C12Q1/6806
摘要： Provided is a method of preparing nucleic acid molecules comprising: (a) a step of providing nucleic acid fragments constituting at least a portion of the complete sequence of a target nucleic acid; (b) tagging the nucleic acid fragments with barcode sequences; (c) identifying the sequence of the nucleic acid fragments tagged by the barcode sequences; and (d) recovering desired nucleic acid fragments among the sequence-identified nucleic acid fragments using the barcode sequences.
摘要翻译：提供了制备核酸分子的方法，包括：（a）提供构成靶核酸的完整序列的至少一部分的核酸片段的步骤; （b）用条形码序列标记核酸片段; （c）鉴定由条形码序列标记的核酸片段的序列; 和（d）使用条形码序列在序列鉴定的核酸片段中回收所需的核酸片段。

8. 发明授权

US09322058B2 Methods for nucleic acid manipulation 有权
公开(公告)号：US09322058B2
公开(公告)日：2016-04-26
申请号：US14268444
申请日：2014-05-02
申请人： THE PENN STATE RESEARCH FOUNDATION
发明人： Stephen J Benkovic , Frank Salinas
IPC分类号： C12Q1/68 , C12N15/10 , C12P19/34
CPC分类号： C12P19/34 , C12N15/1027 , C12Q1/6806 , C12Q1/6844 , C12Q1/686 , C12Q2521/507 , C12Q2521/513 , C12Q2527/125
摘要： A method for replicating and amplifying a target nucleic acid sequence is described. A method of the invention involves the formation of a recombination intermediate without the prior denaturing of a nucleic acid duplex through the use of a recombination factor. The recombination intermediate is treated with a high fidelity polymerase to permit the replication and amplification of the target nucleic acid sequence. In preferred embodiments, the polymerase comprises a polymerase holoenzyme. In further preferred embodiments, the recombination factor is bacteriophage T4 UvsX protein or homologs from other species, and the polymerase holoenzyme comprises a polymerase enzyme, a clamp protein and a clamp loader protein, derived from viral, bacteriophage, prokaryotic, archaebacterial, or eukaryotic systems.

9. 发明申请

US20150329853A1 COMPOSITIONS AND METHODS FOR CREATING ALTERED AND IMPROVED CELLS AND ORGANISMS 审中-公开
标题翻译：用于创建改良和改进的细胞和有机体的组合物和方法
公开(公告)号：US20150329853A1
公开(公告)日：2015-11-19
申请号：US14653840
申请日：2013-12-19
申请人： Helge ZIELER
发明人： Helge ZIELER
IPC分类号： C12N15/10
CPC分类号： C12N15/1082 , C07H21/02 , C12N15/102 , C12N15/1027 , C12N15/70 , C12N15/81
摘要： The present invention provides compositions comprising randomized in-frame fusion polynucleotides and methods for introducing them into a host organism to identify desirable phenotypic changes that disrupt or alter existing genetic or biochemical mechanisms or pathways, thus creating novel characteristics of the transformed organism. Methods for using the compositions for increasing diversity within populations of organisms are also presented.
摘要翻译：本发明提供了包含随机化的帧内融合多核苷酸的组合物和将其引入宿主生物体以鉴定破坏或改变现有遗传或生物化学机制或途径的所需表型变化的方法，从而产生转化生物的新特征。还提出了使用组合物增加生物群体内多样性的方法。

10. 发明申请

US20150203838A1 Methods for Generating Pools of Variants of a DNA Template 审中-公开
标题翻译：生成DNA模板变体池的方法
公开(公告)号：US20150203838A1
公开(公告)日：2015-07-23
申请号：US14420881
申请日：2013-08-09
申请人： The Broad Institute, Inc.
发明人： Tarjei Mikkelsen , Alexandre Melnikov
IPC分类号： C12N15/10
CPC分类号： C12N15/1027 , C12N15/1031 , C12N15/1058 , C12N15/1079
摘要： The invention provides methods for generating pools of variants of DNA templates, and methods of using pools of variants to identify sequences involved in conferring sensitivity or resistance to environmental factors.
摘要翻译：本发明提供了用于产生DNA模板变体池的方法，以及使用变体池来识别赋予对环境因素的敏感性或抗性的序列的方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式