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1. 发明授权

US06709863B2 Nucleic acid molecules encoding multiple start codons and histidine tags 失效
标题翻译：编码多个起始密码子和组氨酸标签的核酸分子
公开(公告)号：US06709863B2
公开(公告)日：2004-03-23
申请号：US09897776
申请日：2001-06-29
申请人： Patrick S. Schnable , Feng Liu , Yan Fu
发明人： Patrick S. Schnable , Feng Liu , Yan Fu
IPC分类号： C12N510
CPC分类号： C12N15/1086 , C07K2319/21 , C12N15/1065 , C12N15/62
摘要： Compositions and methods for identifying nucleotide fragments that contain an open reading frame are provided. Compositions comprise a nucleotide sequence that encodes, in each of the three possible reading frames, an ATG start codon and a histidine tag, and vectors comprising such a nucleotide sequence. The vectors may be provided with cloning sites for insertion of nucleotide sequences of interest 5′ or 3′ to the 3-frame His-tag DNA sequence. Vectors also are provided with cloning sites for inserting nucleotide sequences of interest 3′ of the ATG start codon and 5′ of the 3-frame His-tag DNA sequence.
摘要翻译：提供了用于鉴定包含开放阅读框架的核苷酸片段的组合物和方法。组合物包含在三个可能的阅读框中的每一个中编码ATG起始密码子和组氨酸标签的核苷酸序列，以及包含这种核苷酸序列的载体。可以向载体提供用于将感兴趣的5'或3'的核苷酸序列插入3框架His标签DNA序列的克隆位点。载体还提供有用于插入ATG起始密码子的3'和3框His标签DNA序列的5'的感兴趣的核苷酸序列的克隆位点。

2. 发明授权

US06428961B1 Nucleic acid molecules encoding histidine tags in three reading frames 失效
标题翻译：在三个阅读框中编码组氨酸标签的核酸分子
公开(公告)号：US06428961B1
公开(公告)日：2002-08-06
申请号：US09732990
申请日：2000-12-08
申请人： Patrick S. Schnable , Feng Liu , Yan Fu
发明人： Patrick S. Schnable , Feng Liu , Yan Fu
IPC分类号： C12N514
CPC分类号： C12N15/1065 , C07K2319/21 , C12N15/62
摘要： Compositions and methods for identifying nucleotide fragments that contain an open reading frame are provided. Compositions comprise a nucleotide sequence that encodes histidine tags in each of the three possible reading frames and vectors comprising such a nucleotide sequence. The vectors may be provided with cloning sites for insertion of nucleotide sequences 5′ or 3′ to the 3-frame His-tag DNA sequence.
摘要翻译：提供了用于鉴定包含开放阅读框架的核苷酸片段的组合物和方法。组合物包含在三个可能的阅读框中的每一个中编码组氨酸标签的核苷酸序列和包含这种核苷酸序列的载体。可以向载体提供用于将核苷酸序列5'或3'插入3框架His标签DNA序列的克隆位点。

3. 发明授权

US07056672B2 Method of identifying an open reading frame using a nucleic acid molecule encoding multiple start codons and histidine tags 失效
标题翻译：使用编码多个起始密码子和组氨酸标签的核酸分子鉴定开放阅读框的方法
公开(公告)号：US07056672B2
公开(公告)日：2006-06-06
申请号：US10766102
申请日：2004-01-27
申请人： Patrick S. Schnable , Feng Liu , Yan Fu
发明人： Patrick S. Schnable , Feng Liu , Yan Fu
IPC分类号： C12Q1/68 , C12P21/00 , C12P21/02
CPC分类号： C12N15/1086 , C07K2319/21 , C12N15/1065 , C12N15/62
摘要： Compositions and methods for identifying nucleotide fragments that contain an open reading frame are provided. Compositions comprise a nucleotide sequence that encodes, in each of the three possible reading frames, an ATG start codon and a histidine tag, and vectors comprising such a nucleotide sequence. The vectors may be provided with cloning sites for insertion of nucleotide sequences of interest 5′ or 3′ to the 3-frame His-tag DNA sequence. Vectors also are provided with cloning sites for inserting nucleotide sequences of interest 3′ of the ATG start codon and 5′ of the 3-frame His-tag DNA sequence.
摘要翻译：提供了用于鉴定包含开放阅读框架的核苷酸片段的组合物和方法。组合物包含在三个可能的阅读框中的每一个中编码ATG起始密码子和组氨酸标签的核苷酸序列，以及包含这种核苷酸序列的载体。可以向载体提供用于将感兴趣的5'或3'的核苷酸序列插入3框架His标签DNA序列的克隆位点。载体还提供有用于插入ATG起始密码子的3'和3框His标签DNA序列的5'的感兴趣的核苷酸序列的克隆位点。

4. 发明授权

US6060644A Isolation and use of cuticular lipid genes 失效
标题翻译：分离和使用角质脂质基因
公开(公告)号：US6060644A
公开(公告)日：2000-05-09
申请号：US581148
申请日：1995-12-29
申请人： Patrick S. Schnable , Donald S. Robertson , Joel D. Hansen , Basil J. Nikolau , Xiaojie Xu , Yiji Xia
发明人： Patrick S. Schnable , Donald S. Robertson , Joel D. Hansen , Basil J. Nikolau , Xiaojie Xu , Yiji Xia
IPC分类号： C07K14/415 , C12N9/00 , C12N9/10 , C12N15/82 , A01H5/00 , C12N5/04
CPC分类号： C07K14/415 , C12N15/8247 , C12N15/8279 , C12N9/00 , C12N9/1029
摘要： The present invention relates to cuticular lipid genes, their gene products, and methods of use thereof, for the generation of new plant varieties having novel environmental, disease and pest resistance. In particular, certain isolated or enriched nucleic acids specifying cuticular lipid genes, or portions thereof, are disclosed.
摘要翻译：本发明涉及角质层脂质基因，其基因产物及其使用方法，用于产生具有新型环境，疾病和害虫抗性的新植物品种。特别地，公开了指定角质层脂质基因的某些分离或富集的核酸或其部分。

5. 发明授权

US5981833A Nuclear restorer genes for hybrid seed production 失效
标题翻译：用于杂交种子生产的核恢复基因
公开(公告)号：US5981833A
公开(公告)日：1999-11-09
申请号：US794494
申请日：1997-02-04
申请人： Roger P. Wise , Patrick S. Schnable
发明人： Roger P. Wise , Patrick S. Schnable
IPC分类号： C07K14/415 , C12N15/82 , A01H5/00 , A01H1/00 , C12N15/00
CPC分类号： C07K14/415 , C12N15/8289
摘要： The present invention relates to the field of producing hybrid seeds, and specifically relates to the application of certain cloned nuclear fertility restorer genes, which restore male fertility to cytoplasmic male-sterile plants. In particular, the present invention relates to an enriched or substantially isolated nucleic acid sequence comprising a nucleotide sequence that hybridizes at least under moderately stringent conditions to SEQ ID NOS: 1, 3 or 4 and restores male fertility to a cytoplasmic male-sterile plant; a vector comprising such a sequence; an organism, virus, seed, plant cell, and plant comprising such a vector; methods for the production of hybrid seed; a method of producing a variant of a cytoplasmic male-sterile plant, and a method of suppressing cytoplasmic male sterility, all of which make use of the aforementioned nucleic acid sequences.
摘要翻译：本发明涉及杂交种子的生产领域，具体涉及某些克隆的核生育恢复基因的应用，其将雄性育性恢复至细胞质雄性不育植物。特别地，本发明涉及富含或基本上分离的核酸序列，其包含至少在中度严格条件下与SEQ ID NO：1,3或4杂交的核苷酸序列，并将雄性生育力恢复到细胞质雄性不育植物; 包含这样的序列的载体; 生物体，病毒，种子，植物细胞和包含这种载体的植物; 杂交种子的生产方法; 产生细胞质雄性不育植物的变体的方法和抑制细胞质雄性不育的方法，所有这些都利用上述核酸序列。

6. 发明授权

US5684242A Nuclear restorer genes for hybrid seed production 失效
标题翻译：用于杂交种子生产的核恢复基因
公开(公告)号：US5684242A
公开(公告)日：1997-11-04
申请号：US346611
申请日：1994-11-29
申请人： Patrick S. Schnable , Roger P. Wise
发明人： Patrick S. Schnable , Roger P. Wise
IPC分类号： C07K14/415 , C12N15/82 , C12N15/00 , A01H1/06 , A01H11/00
CPC分类号： C07K14/415 , C12N15/8289
摘要： The present invention relates to the field of producing hybrid seeds, and specifically relates to the application of certain cloned nuclear restorer genes that are active in reversing cytoplasmic male sterile systems. In particular, the present invention relates to: certain polynucleotide sequences that hybridize to nuclear restorer genes of plants, vectors, organisms, seeds, plant cells, and plants that comprise such polynucleotide sequences; a novel method for the production of hybrid seed that uses the aforementioned polynucleotide sequence; and a second novel method for the production of variant cytoplasmic male sterile factors.
摘要翻译：本发明涉及杂交种子的生产领域，特别涉及在逆转细胞质雄性不育系统中有活性的某些克隆的核恢复基因的应用。特别地，本发明涉及：与包含这种多核苷酸序列的植物，载体，生物体，种子，植物细胞和植物的核恢复基因杂交的某些多核苷酸序列; 用于生产使用上述多核苷酸序列的杂种种子的新方法; 以及用于产生变体细胞质雄性不育因子的第二种新方法。

7. 发明授权

US08779233B1 QTL regulating ear productivity traits in maize 有权
标题翻译： QTL调控玉米的生产力特征
公开(公告)号：US08779233B1
公开(公告)日：2014-07-15
申请号：US13180986
申请日：2011-07-12
申请人： Patrick S. Schnable , Jinliang Yang , Ruth A. Swanson-Wagner , Dan Nettleton
发明人： Patrick S. Schnable , Jinliang Yang , Ruth A. Swanson-Wagner , Dan Nettleton
IPC分类号： A01H1/04
CPC分类号： A01H1/04 , A01H5/10
摘要： The present invention relates to a method for determining an ear productivity trait in maize, which involves analyzing genomic DNA from a maize plant, germplasm, pollen, or seed for the presence of a molecular marker linked to a QTL associated with an ear productivity trait in maize. Also disclosed are methods of selecting a maize plant with a desired ear productivity trait; reliably and predictably introgressing an improved ear productivity trait into a maize line; and producing a maize line having a desired ear productivity trait. A kit for selecting a maize plant by marker assisted selection of a QTL associated with a desired ear productivity trait; an isolated nucleic acid comprising a QTL associated with an ear productivity trait in maize; and a transgenic plant comprising a recombinant nucleic acid genetically linked to a locus in maize, are also disclosed.
摘要翻译：本发明涉及一种用于确定玉米的穗生产力性状的方法，其涉及分析来自玉米植物，种质，花粉或种子的基因组DNA，以存在与耳朵生产力性状相关的QTL连锁的分子标记玉米。还公开了选择具有期望的耳朵生产力性状的玉米植物的方法; 可靠和可预测地将改良的耳朵生产力特征引入玉米系; 并生产具有所需耳朵生产力性状的玉米品系。用于通过标记辅助选择与期望的耳朵生产力性状相关联的QTL来选择玉米植物的试剂盒; 分离的核酸，其包含与玉米的耳朵生产性状相关的QTL; 还公开了包含与玉米基因座遗传连锁的重组核酸的转基因植物。

8. 发明授权

US07524678B2 Materials and methods for the alteration of enzyme and acetyl CoA levels in plants 失效
标题翻译：用于改变植物中酶和乙酰CoA水平的材料和方法
公开(公告)号：US07524678B2
公开(公告)日：2009-04-28
申请号：US11167856
申请日：2005-06-27
申请人： Basil J. Nikolau , Eve S. Wurtele , David J. Oliver , Patrick S. Schnable , Tsui-Jung Wen
发明人： Basil J. Nikolau , Eve S. Wurtele , David J. Oliver , Patrick S. Schnable , Tsui-Jung Wen
IPC分类号： C12N5/10
CPC分类号： C12N15/1137 , C12N9/0008 , C12N9/0051 , C12N9/1029 , C12N9/16 , C12N9/88 , C12N9/93 , C12N15/8243
摘要： The present invention provides nucleic acid and amino acid sequences of acetyl CoA synthetase (ACS), plastidic pyruvate dehydrogenase (pPDH), ATP citrate lyase (ACL), Arabidopsis pyruvate decarboxylase (PDC), and Arabidopsis aldehyde dehydrogenase (ALDH), specifically ALDH-2 and ALDH-4. The present invention also provides a recombinant vector comprising a nucleic acid sequence encoding one of the aforementioned enzymes, an antisense sequence thereto or a ribozyme therefor, a cell transformed with such a vector, antibodies to the enzymes, a plant cell, a plant tissue, a plant organ or a plant in which the level of an enzyme has been altered, and a method of producing such a plant cell, plant tissue, plant organ or plant. Desirably, alteration of the level of enzyme results in an alteration of the level of acetyl CoA in the plant cell, plant tissue, plant organ or plant. In addition, the present invention provides a recombinant vector comprising an antisense sequence of a nucleic acid sequence encoding pyruvate decarboxylase (PDC), the E1α subunit of pPDH, the E1β subunit of pPDH, the E2 subunit of pPDH, mitochondrial pyruvate dehydrogenase (mtPDH) or aldehyde dehydrogenase (ALDH) or a ribozyme that can cleave an RNA molecule encoding PDC, E1α pPDH, E1β pPDH, E2 pPDH, mtPDH or ALDH.
摘要翻译：本发明提供乙酰辅酶A合成酶（ACS），质体丙酮酸脱氢酶（pPDH），ATP柠檬酸裂合酶（ACL），拟南芥丙酮酸脱羧酶（PDC）和拟南芥醛脱氢酶（ALDH），特别是ALDH- 2和ALDH-4。本发明还提供了一种重组载体，其包含编码上述酶之一的核酸序列，其上的反义序列或其核酶，用该载体转化的细胞，对酶的抗体，植物细胞，植物组织，其中酶的水平已被改变的植物器官或植物，以及生产这种植物细胞，植物组织，植物器官或植物的方法。理想地，酶水平的改变导致植物细胞，植物组织，植物器官或植物中乙酰CoA水平的改变。此外，本发明提供了包含编码丙酮酸脱羧酶（PDC），pPDH的E1alpha亚基，pPDH的E1beta亚基，pPDH的E2亚基，线粒体丙酮酸脱氢酶（mtPDH）的核酸序列的反义序列的重组载体，或醛脱氢酶（ALDH）或可以切割编码PDC，E1alpha pPDH，E1beta pPDH，E2pPDH，mtPDH或ALDH的RNA分子的核酶。

9. 发明授权

US06942994B2 Materials and methods for the alteration of enzyme and acetyl CoA levels in plants 失效
标题翻译：用于改变植物中酶和乙酰CoA水平的材料和方法
公开(公告)号：US06942994B2
公开(公告)日：2005-09-13
申请号：US10293865
申请日：2002-11-13
申请人： Basil J. Nikolau , Eve S. Wurtele , David J. Oliver , Robert Behal , Patrick S. Schnable , Jinshan Ke , Jerry L. Johnson , Carolyn C. Allred , Beth Fatland , Isabelle Lutziger , Tsui-Jung Wen
发明人： Basil J. Nikolau , Eve S. Wurtele , David J. Oliver , Robert Behal , Patrick S. Schnable , Jinshan Ke , Jerry L. Johnson , Carolyn C. Allred , Beth Fatland , Isabelle Lutziger , Tsui-Jung Wen
IPC分类号： A01H1/00 , C12N5/04 , C12N9/00 , C12N9/02 , C12N9/10 , C12N9/16 , C12N9/88 , C12N15/113 , C12N15/82 , C12N15/09 , A01H5/00 , C12N5/10
CPC分类号： C12N15/1137 , C12N9/0008 , C12N9/0051 , C12N9/1029 , C12N9/16 , C12N9/88 , C12N9/93 , C12N15/8243
摘要： The present invention provides nucleic acid and amino acid sequences of acetyl CoA synthetase (ACS), plastidic pyruvate dehydrogenase (pPDH), ATP citrate lyase (ACL), Arabidopsis pyruvate decarboxylase (PDC), and Arabidopsis aldehyde dehydrogenase (ALDH), specifically ALDH-2 and ALDH-4. The present invention also provides a recombinant vector comprising a nucleic acid sequence encoding one of the aforementioned enzymes, an antisense sequence thereto or a ribozyme therefor, a cell transformed with such a vector, antibodies to the enzymes, a plant cell, a plant tissue, a plant organ or a plant in which the level of an enzyme has been altered, and a method of producing such a plant cell, plant tissue, plant organ or plant. Desirably, alteration of the level of enzyme results in an alteration of the level of acetyl CoA in the plant cell, plant tissue, plant organ or plant. In addition, the present invention provides a recombinant vector comprising an antisense sequence of a nucleic acid sequence encoding pyruvate decarboxylase (PDC), the E1α subunit of pPDH, the E1β subunit of pPDH, the E2 subunit of pPDH, mitochondrial pyruvate dehydrogenase (mtPDH) or aldehyde dehydrogenase (ALDH) or a ribozyme that can cleave an RNA molecule encoding PDC, E1α pPDH, E1β pPDH, E2 pPDH, mtPDH or ALDH.
摘要翻译：本发明提供乙酰辅酶A合成酶（ACS），质体丙酮酸脱氢酶（pPDH），ATP柠檬酸裂解酶（ACL），拟南芥丙酮酸脱羧酶（PDC）和拟南芥醛脱氢酶（ALDH），特别是ALDH- 2和ALDH-4。本发明还提供了一种重组载体，其包含编码上述酶之一的核酸序列，其上的反义序列或其核酶，用该载体转化的细胞，对酶的抗体，植物细胞，植物组织，其中酶的水平已被改变的植物器官或植物，以及生产这种植物细胞，植物组织，植物器官或植物的方法。理想地，酶水平的改变导致植物细胞，植物组织，植物器官或植物中乙酰CoA水平的改变。此外，本发明提供了包含编码丙酮酸脱羧酶（PDC），pPDH的E1alpha亚基，pPDH的E1beta亚基，pPDH的E2亚基，线粒体丙酮酸脱氢酶（mtPDH）的核酸序列的反义序列的重组载体，或醛脱氢酶（ALDH）或可切割编码PDC，E1alpha pPDH，E1beta pPDH，E2pPDH，mtPDH或ALDH的RNA分子的核酶。

10. 发明申请

US20130184165A1 GENOTYPING BY NEXT-GENERATION SEQUENCING 审中-公开
公开(公告)号：US20130184165A1
公开(公告)日：2013-07-18
申请号：US13739874
申请日：2013-01-11
申请人： Patrick S. Schnable , Sanzhen Liu , Wei Wu
发明人： Patrick S. Schnable , Sanzhen Liu , Wei Wu
IPC分类号： C12N15/10 , C12Q1/68
CPC分类号： C12Q1/6874 , C12N15/1065 , C12N15/1093 , C12Q1/6806 , C12Q1/683 , C12Q1/6869 , C12Q2521/301 , C12Q2525/155 , C12Q2525/191 , C12Q2535/122 , C12Q2537/159 , C12Q2565/514
摘要： Provided herein is technology relating to genotyping and particularly, but not exclusively, to methods for genotyping one or more organisms by genome sequencing.

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