会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 3. 发明授权
    • Materials and methods for the alteration of enzyme and acetyl CoA levels in plants
    • 用于改变植物中酶和乙酰CoA水平的材料和方法
    • US07524678B2
    • 2009-04-28
    • US11167856
    • 2005-06-27
    • Basil J. NikolauEve S. WurteleDavid J. OliverPatrick S. SchnableTsui-Jung Wen
    • Basil J. NikolauEve S. WurteleDavid J. OliverPatrick S. SchnableTsui-Jung Wen
    • C12N5/10
    • C12N15/1137C12N9/0008C12N9/0051C12N9/1029C12N9/16C12N9/88C12N9/93C12N15/8243
    • The present invention provides nucleic acid and amino acid sequences of acetyl CoA synthetase (ACS), plastidic pyruvate dehydrogenase (pPDH), ATP citrate lyase (ACL), Arabidopsis pyruvate decarboxylase (PDC), and Arabidopsis aldehyde dehydrogenase (ALDH), specifically ALDH-2 and ALDH-4. The present invention also provides a recombinant vector comprising a nucleic acid sequence encoding one of the aforementioned enzymes, an antisense sequence thereto or a ribozyme therefor, a cell transformed with such a vector, antibodies to the enzymes, a plant cell, a plant tissue, a plant organ or a plant in which the level of an enzyme has been altered, and a method of producing such a plant cell, plant tissue, plant organ or plant. Desirably, alteration of the level of enzyme results in an alteration of the level of acetyl CoA in the plant cell, plant tissue, plant organ or plant. In addition, the present invention provides a recombinant vector comprising an antisense sequence of a nucleic acid sequence encoding pyruvate decarboxylase (PDC), the E1α subunit of pPDH, the E1β subunit of pPDH, the E2 subunit of pPDH, mitochondrial pyruvate dehydrogenase (mtPDH) or aldehyde dehydrogenase (ALDH) or a ribozyme that can cleave an RNA molecule encoding PDC, E1α pPDH, E1β pPDH, E2 pPDH, mtPDH or ALDH.
    • 本发明提供乙酰辅酶A合成酶(ACS),质体丙酮酸脱氢酶(pPDH),ATP柠檬酸裂合酶(ACL),拟南芥丙酮酸脱羧酶(PDC)和拟南芥醛脱氢酶(ALDH),特别是ALDH- 2和ALDH-4。 本发明还提供了一种重组载体,其包含编码上述酶之一的核酸序列,其上的反义序列或其核酶,用该载体转化的细胞,对酶的抗体,植物细胞,植物组织, 其中酶的水平已被改变的植物器官或植物,以及生产这种植物细胞,植物组织,植物器官或植物的方法。 理想地,酶水平的改变导致植物细胞,植物组织,植物器官或植物中乙酰CoA水平的改变。 此外,本发明提供了包含编码丙酮酸脱羧酶(PDC),pPDH的E1alpha亚基,pPDH的E1beta亚基,pPDH的E2亚基,线粒体丙酮酸脱氢酶(mtPDH)的核酸序列的反义序列的重组载体, 或醛脱氢酶(ALDH)或可以切割编码PDC,E1alpha pPDH,E1beta pPDH,E2pPDH,mtPDH或ALDH的RNA分子的核酶。