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1. 发明授权

US06764851B2 Materials and methods for the alteration of enzyme and acetyl CoA levels in plants 失效
标题翻译：用于改变植物中酶和乙酰CoA水平的材料和方法
公开(公告)号：US06764851B2
公开(公告)日：2004-07-20
申请号：US09344882
申请日：1999-06-25
申请人： Basil J. Nikolau , Eve S. Wurtele , David J. Oliver , Robert Behal , Patrick S. Schnable , Jinshan Ke , Jerry L. Johnson , Carolyn C. Allred , Beth Fatland , Isabelle Lutziger , Tsui-Jung Wen
发明人： Basil J. Nikolau , Eve S. Wurtele , David J. Oliver , Robert Behal , Patrick S. Schnable , Jinshan Ke , Jerry L. Johnson , Carolyn C. Allred , Beth Fatland , Isabelle Lutziger , Tsui-Jung Wen
IPC分类号： C12N1574
CPC分类号： C12N15/1137 , C12N9/0008 , C12N9/0051 , C12N9/1029 , C12N9/16 , C12N9/88 , C12N9/93 , C12N15/8243
摘要： The present invention provides nucleic acid and amino acid sequences of acetyl CoA synthetase (ACS), plastidic pyruvate dehydrogenase (pPDH), ATP citrate lyase (ACL), Arabidopsis pyruvate decarboxylase (PDC), and Arabidopsis aldehyde dehydrogenase (ALDH), specifically ALDH-2 and ALDH-4. The present invention also provides a recombinant vector comprising a nucleic acid sequence encoding one of the aforementioned enzymes, an antisense sequence thereto or a ribozyme therefor, a cell transformed with such a vector, antibodies to the enzymes, a plant cell, a plant tissue, a plant organ or a plant in which the level of an enzyme has been altered, and a method of producing such a plant cell, plant tissue, plant organ or plant. Desirably, alteration of the level of enzyme results in an alteration of the level of acetyl CoA in the plant cell, plant tissue, plant organ or plant. In addition, the present invention provides a recombinant vector comprising an antisense sequence of a nucleic acid sequence encoding pyruvate decarboxylase (PDC), the E1&agr;subunit of pPDH, the E1&bgr;subunit of pPDH, the E2 subunit of pPDH, mitochondrial pyurvate dehydrogenase (mtPDH) or aldehyde dehydrogenase (ALDH) or a ribozyme that can cleave an RNA molecule encoding PDC, E1&agr;pPDH, E1&bgr;pPDH, E2 pPDH, mtPDH or ALDH.
摘要翻译：本发明提供乙酰辅酶A合成酶（ACS），质体丙酮酸脱氢酶（pPDH），ATP柠檬酸裂解酶（ACL），拟南芥丙酮酸脱羧酶（PDC）和拟南芥醛脱氢酶（ALDH），特别是ALDH- 2和ALDH-4。本发明还提供了一种重组载体，其包含编码上述酶之一的核酸序列，其上的反义序列或其核酶，用该载体转化的细胞，对酶的抗体，植物细胞，植物组织，其中酶的水平已被改变的植物器官或植物，以及生产这种植物细胞，植物组织，植物器官或植物的方法。理想地，酶水平的改变导致植物细胞，植物组织，植物器官或植物中乙酰CoA水平的改变。此外，本发明提供了包含编码丙酮酸脱羧酶（PDC）的核酸序列，pPDH的E1a亚单位，pPDH的E1betubunit，pPDH的E2亚基，线粒体高磷酸脱氢酶（mtPDH）或醛的反义序列的重组载体脱氢酶（ALDH）或可以切割编码PDC，E1alphapPDH，E1betapPDH，E2pPDH，mtPDH或ALDH的RNA分子的核酶。

2. 发明授权

US07524678B2 Materials and methods for the alteration of enzyme and acetyl CoA levels in plants 失效
标题翻译：用于改变植物中酶和乙酰CoA水平的材料和方法
公开(公告)号：US07524678B2
公开(公告)日：2009-04-28
申请号：US11167856
申请日：2005-06-27
申请人： Basil J. Nikolau , Eve S. Wurtele , David J. Oliver , Patrick S. Schnable , Tsui-Jung Wen
发明人： Basil J. Nikolau , Eve S. Wurtele , David J. Oliver , Patrick S. Schnable , Tsui-Jung Wen
IPC分类号： C12N5/10
CPC分类号： C12N15/1137 , C12N9/0008 , C12N9/0051 , C12N9/1029 , C12N9/16 , C12N9/88 , C12N9/93 , C12N15/8243
摘要： The present invention provides nucleic acid and amino acid sequences of acetyl CoA synthetase (ACS), plastidic pyruvate dehydrogenase (pPDH), ATP citrate lyase (ACL), Arabidopsis pyruvate decarboxylase (PDC), and Arabidopsis aldehyde dehydrogenase (ALDH), specifically ALDH-2 and ALDH-4. The present invention also provides a recombinant vector comprising a nucleic acid sequence encoding one of the aforementioned enzymes, an antisense sequence thereto or a ribozyme therefor, a cell transformed with such a vector, antibodies to the enzymes, a plant cell, a plant tissue, a plant organ or a plant in which the level of an enzyme has been altered, and a method of producing such a plant cell, plant tissue, plant organ or plant. Desirably, alteration of the level of enzyme results in an alteration of the level of acetyl CoA in the plant cell, plant tissue, plant organ or plant. In addition, the present invention provides a recombinant vector comprising an antisense sequence of a nucleic acid sequence encoding pyruvate decarboxylase (PDC), the E1α subunit of pPDH, the E1β subunit of pPDH, the E2 subunit of pPDH, mitochondrial pyruvate dehydrogenase (mtPDH) or aldehyde dehydrogenase (ALDH) or a ribozyme that can cleave an RNA molecule encoding PDC, E1α pPDH, E1β pPDH, E2 pPDH, mtPDH or ALDH.
摘要翻译：本发明提供乙酰辅酶A合成酶（ACS），质体丙酮酸脱氢酶（pPDH），ATP柠檬酸裂合酶（ACL），拟南芥丙酮酸脱羧酶（PDC）和拟南芥醛脱氢酶（ALDH），特别是ALDH- 2和ALDH-4。本发明还提供了一种重组载体，其包含编码上述酶之一的核酸序列，其上的反义序列或其核酶，用该载体转化的细胞，对酶的抗体，植物细胞，植物组织，其中酶的水平已被改变的植物器官或植物，以及生产这种植物细胞，植物组织，植物器官或植物的方法。理想地，酶水平的改变导致植物细胞，植物组织，植物器官或植物中乙酰CoA水平的改变。此外，本发明提供了包含编码丙酮酸脱羧酶（PDC），pPDH的E1alpha亚基，pPDH的E1beta亚基，pPDH的E2亚基，线粒体丙酮酸脱氢酶（mtPDH）的核酸序列的反义序列的重组载体，或醛脱氢酶（ALDH）或可以切割编码PDC，E1alpha pPDH，E1beta pPDH，E2pPDH，mtPDH或ALDH的RNA分子的核酶。

3. 发明授权

US06942994B2 Materials and methods for the alteration of enzyme and acetyl CoA levels in plants 失效
标题翻译：用于改变植物中酶和乙酰CoA水平的材料和方法
公开(公告)号：US06942994B2
公开(公告)日：2005-09-13
申请号：US10293865
申请日：2002-11-13
申请人： Basil J. Nikolau , Eve S. Wurtele , David J. Oliver , Robert Behal , Patrick S. Schnable , Jinshan Ke , Jerry L. Johnson , Carolyn C. Allred , Beth Fatland , Isabelle Lutziger , Tsui-Jung Wen
发明人： Basil J. Nikolau , Eve S. Wurtele , David J. Oliver , Robert Behal , Patrick S. Schnable , Jinshan Ke , Jerry L. Johnson , Carolyn C. Allred , Beth Fatland , Isabelle Lutziger , Tsui-Jung Wen
IPC分类号： A01H1/00 , C12N5/04 , C12N9/00 , C12N9/02 , C12N9/10 , C12N9/16 , C12N9/88 , C12N15/113 , C12N15/82 , C12N15/09 , A01H5/00 , C12N5/10
CPC分类号： C12N15/1137 , C12N9/0008 , C12N9/0051 , C12N9/1029 , C12N9/16 , C12N9/88 , C12N9/93 , C12N15/8243
摘要： The present invention provides nucleic acid and amino acid sequences of acetyl CoA synthetase (ACS), plastidic pyruvate dehydrogenase (pPDH), ATP citrate lyase (ACL), Arabidopsis pyruvate decarboxylase (PDC), and Arabidopsis aldehyde dehydrogenase (ALDH), specifically ALDH-2 and ALDH-4. The present invention also provides a recombinant vector comprising a nucleic acid sequence encoding one of the aforementioned enzymes, an antisense sequence thereto or a ribozyme therefor, a cell transformed with such a vector, antibodies to the enzymes, a plant cell, a plant tissue, a plant organ or a plant in which the level of an enzyme has been altered, and a method of producing such a plant cell, plant tissue, plant organ or plant. Desirably, alteration of the level of enzyme results in an alteration of the level of acetyl CoA in the plant cell, plant tissue, plant organ or plant. In addition, the present invention provides a recombinant vector comprising an antisense sequence of a nucleic acid sequence encoding pyruvate decarboxylase (PDC), the E1α subunit of pPDH, the E1β subunit of pPDH, the E2 subunit of pPDH, mitochondrial pyruvate dehydrogenase (mtPDH) or aldehyde dehydrogenase (ALDH) or a ribozyme that can cleave an RNA molecule encoding PDC, E1α pPDH, E1β pPDH, E2 pPDH, mtPDH or ALDH.
摘要翻译：本发明提供乙酰辅酶A合成酶（ACS），质体丙酮酸脱氢酶（pPDH），ATP柠檬酸裂解酶（ACL），拟南芥丙酮酸脱羧酶（PDC）和拟南芥醛脱氢酶（ALDH），特别是ALDH- 2和ALDH-4。本发明还提供了一种重组载体，其包含编码上述酶之一的核酸序列，其上的反义序列或其核酶，用该载体转化的细胞，对酶的抗体，植物细胞，植物组织，其中酶的水平已被改变的植物器官或植物，以及生产这种植物细胞，植物组织，植物器官或植物的方法。理想地，酶水平的改变导致植物细胞，植物组织，植物器官或植物中乙酰CoA水平的改变。此外，本发明提供了包含编码丙酮酸脱羧酶（PDC），pPDH的E1alpha亚基，pPDH的E1beta亚基，pPDH的E2亚基，线粒体丙酮酸脱氢酶（mtPDH）的核酸序列的反义序列的重组载体，或醛脱氢酶（ALDH）或可切割编码PDC，E1alpha pPDH，E1beta pPDH，E2pPDH，mtPDH或ALDH的RNA分子的核酶。

4. 发明授权

US5633363A Root preferential promoter 失效
标题翻译：根优先启动子
公开(公告)号：US5633363A
公开(公告)日：1997-05-27
申请号：US253785
申请日：1994-06-03
申请人： James T. Colbert , Bruce M. Held , Eve S. Wurtele , Paul S. Dietrich
发明人： James T. Colbert , Bruce M. Held , Eve S. Wurtele , Paul S. Dietrich
IPC分类号： C07K14/415 , C12N15/82 , C12N15/11 , A01H5/00 , C12N15/29
CPC分类号： C12N15/8227 , C07K14/415
摘要： The present invention is directed to promoter fragments isolated from maize and particularly to a 4.7 kbp upstream promoter region designated ZRP2 of a ZRP2 genomic DNA clone, specific fragments thereof designated ZRP2(2.0) and ZRF2(1.0) and functional equivalents thereto. The promoter has particular utility in driving root preferential expression of heterologous genes that impart increased agronomic, horticultural and/or pesticidal characteristics to a given plant. This invention is also directed to DNA molecules including said promoters and transformed plant tissue including a DNA molecule comprising a promoter of the invention operably linked to a heterologous gene or genes and the seeds thereof.
摘要翻译：本发明涉及从玉米分离的启动子片段，特别涉及ZRP2基因组DNA克隆的ZRP2的4.7kbp上游启动子区域，其特定片段称为ZRP2（2.0）和ZRF2（1.0）及其功能等同物。启动子在驱动给定植物赋予增加的农艺，园艺和/或杀虫特性的异源基因的根优先表达方面具有特别的用途。本发明还涉及包括所述启动子和转化植物组织的DNA分子，其包括可操作地连接到异源基因或其基因的本发明启动子的DNA分子及其种子。

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