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1. 发明授权

US06764851B2 Materials and methods for the alteration of enzyme and acetyl CoA levels in plants 失效
标题翻译：用于改变植物中酶和乙酰CoA水平的材料和方法
公开(公告)号：US06764851B2
公开(公告)日：2004-07-20
申请号：US09344882
申请日：1999-06-25
申请人： Basil J. Nikolau , Eve S. Wurtele , David J. Oliver , Robert Behal , Patrick S. Schnable , Jinshan Ke , Jerry L. Johnson , Carolyn C. Allred , Beth Fatland , Isabelle Lutziger , Tsui-Jung Wen
发明人： Basil J. Nikolau , Eve S. Wurtele , David J. Oliver , Robert Behal , Patrick S. Schnable , Jinshan Ke , Jerry L. Johnson , Carolyn C. Allred , Beth Fatland , Isabelle Lutziger , Tsui-Jung Wen
IPC分类号： C12N1574
CPC分类号： C12N15/1137 , C12N9/0008 , C12N9/0051 , C12N9/1029 , C12N9/16 , C12N9/88 , C12N9/93 , C12N15/8243
摘要： The present invention provides nucleic acid and amino acid sequences of acetyl CoA synthetase (ACS), plastidic pyruvate dehydrogenase (pPDH), ATP citrate lyase (ACL), Arabidopsis pyruvate decarboxylase (PDC), and Arabidopsis aldehyde dehydrogenase (ALDH), specifically ALDH-2 and ALDH-4. The present invention also provides a recombinant vector comprising a nucleic acid sequence encoding one of the aforementioned enzymes, an antisense sequence thereto or a ribozyme therefor, a cell transformed with such a vector, antibodies to the enzymes, a plant cell, a plant tissue, a plant organ or a plant in which the level of an enzyme has been altered, and a method of producing such a plant cell, plant tissue, plant organ or plant. Desirably, alteration of the level of enzyme results in an alteration of the level of acetyl CoA in the plant cell, plant tissue, plant organ or plant. In addition, the present invention provides a recombinant vector comprising an antisense sequence of a nucleic acid sequence encoding pyruvate decarboxylase (PDC), the E1&agr;subunit of pPDH, the E1&bgr;subunit of pPDH, the E2 subunit of pPDH, mitochondrial pyurvate dehydrogenase (mtPDH) or aldehyde dehydrogenase (ALDH) or a ribozyme that can cleave an RNA molecule encoding PDC, E1&agr;pPDH, E1&bgr;pPDH, E2 pPDH, mtPDH or ALDH.
摘要翻译：本发明提供乙酰辅酶A合成酶（ACS），质体丙酮酸脱氢酶（pPDH），ATP柠檬酸裂解酶（ACL），拟南芥丙酮酸脱羧酶（PDC）和拟南芥醛脱氢酶（ALDH），特别是ALDH- 2和ALDH-4。本发明还提供了一种重组载体，其包含编码上述酶之一的核酸序列，其上的反义序列或其核酶，用该载体转化的细胞，对酶的抗体，植物细胞，植物组织，其中酶的水平已被改变的植物器官或植物，以及生产这种植物细胞，植物组织，植物器官或植物的方法。理想地，酶水平的改变导致植物细胞，植物组织，植物器官或植物中乙酰CoA水平的改变。此外，本发明提供了包含编码丙酮酸脱羧酶（PDC）的核酸序列，pPDH的E1a亚单位，pPDH的E1betubunit，pPDH的E2亚基，线粒体高磷酸脱氢酶（mtPDH）或醛的反义序列的重组载体脱氢酶（ALDH）或可以切割编码PDC，E1alphapPDH，E1betapPDH，E2pPDH，mtPDH或ALDH的RNA分子的核酶。

2. 发明授权

US07524678B2 Materials and methods for the alteration of enzyme and acetyl CoA levels in plants 失效
标题翻译：用于改变植物中酶和乙酰CoA水平的材料和方法
公开(公告)号：US07524678B2
公开(公告)日：2009-04-28
申请号：US11167856
申请日：2005-06-27
申请人： Basil J. Nikolau , Eve S. Wurtele , David J. Oliver , Patrick S. Schnable , Tsui-Jung Wen
发明人： Basil J. Nikolau , Eve S. Wurtele , David J. Oliver , Patrick S. Schnable , Tsui-Jung Wen
IPC分类号： C12N5/10
CPC分类号： C12N15/1137 , C12N9/0008 , C12N9/0051 , C12N9/1029 , C12N9/16 , C12N9/88 , C12N9/93 , C12N15/8243
摘要： The present invention provides nucleic acid and amino acid sequences of acetyl CoA synthetase (ACS), plastidic pyruvate dehydrogenase (pPDH), ATP citrate lyase (ACL), Arabidopsis pyruvate decarboxylase (PDC), and Arabidopsis aldehyde dehydrogenase (ALDH), specifically ALDH-2 and ALDH-4. The present invention also provides a recombinant vector comprising a nucleic acid sequence encoding one of the aforementioned enzymes, an antisense sequence thereto or a ribozyme therefor, a cell transformed with such a vector, antibodies to the enzymes, a plant cell, a plant tissue, a plant organ or a plant in which the level of an enzyme has been altered, and a method of producing such a plant cell, plant tissue, plant organ or plant. Desirably, alteration of the level of enzyme results in an alteration of the level of acetyl CoA in the plant cell, plant tissue, plant organ or plant. In addition, the present invention provides a recombinant vector comprising an antisense sequence of a nucleic acid sequence encoding pyruvate decarboxylase (PDC), the E1α subunit of pPDH, the E1β subunit of pPDH, the E2 subunit of pPDH, mitochondrial pyruvate dehydrogenase (mtPDH) or aldehyde dehydrogenase (ALDH) or a ribozyme that can cleave an RNA molecule encoding PDC, E1α pPDH, E1β pPDH, E2 pPDH, mtPDH or ALDH.
摘要翻译：本发明提供乙酰辅酶A合成酶（ACS），质体丙酮酸脱氢酶（pPDH），ATP柠檬酸裂合酶（ACL），拟南芥丙酮酸脱羧酶（PDC）和拟南芥醛脱氢酶（ALDH），特别是ALDH- 2和ALDH-4。本发明还提供了一种重组载体，其包含编码上述酶之一的核酸序列，其上的反义序列或其核酶，用该载体转化的细胞，对酶的抗体，植物细胞，植物组织，其中酶的水平已被改变的植物器官或植物，以及生产这种植物细胞，植物组织，植物器官或植物的方法。理想地，酶水平的改变导致植物细胞，植物组织，植物器官或植物中乙酰CoA水平的改变。此外，本发明提供了包含编码丙酮酸脱羧酶（PDC），pPDH的E1alpha亚基，pPDH的E1beta亚基，pPDH的E2亚基，线粒体丙酮酸脱氢酶（mtPDH）的核酸序列的反义序列的重组载体，或醛脱氢酶（ALDH）或可以切割编码PDC，E1alpha pPDH，E1beta pPDH，E2pPDH，mtPDH或ALDH的RNA分子的核酶。

3. 发明授权

US06942994B2 Materials and methods for the alteration of enzyme and acetyl CoA levels in plants 失效
标题翻译：用于改变植物中酶和乙酰CoA水平的材料和方法
公开(公告)号：US06942994B2
公开(公告)日：2005-09-13
申请号：US10293865
申请日：2002-11-13
申请人： Basil J. Nikolau , Eve S. Wurtele , David J. Oliver , Robert Behal , Patrick S. Schnable , Jinshan Ke , Jerry L. Johnson , Carolyn C. Allred , Beth Fatland , Isabelle Lutziger , Tsui-Jung Wen
发明人： Basil J. Nikolau , Eve S. Wurtele , David J. Oliver , Robert Behal , Patrick S. Schnable , Jinshan Ke , Jerry L. Johnson , Carolyn C. Allred , Beth Fatland , Isabelle Lutziger , Tsui-Jung Wen
IPC分类号： A01H1/00 , C12N5/04 , C12N9/00 , C12N9/02 , C12N9/10 , C12N9/16 , C12N9/88 , C12N15/113 , C12N15/82 , C12N15/09 , A01H5/00 , C12N5/10
CPC分类号： C12N15/1137 , C12N9/0008 , C12N9/0051 , C12N9/1029 , C12N9/16 , C12N9/88 , C12N9/93 , C12N15/8243
摘要： The present invention provides nucleic acid and amino acid sequences of acetyl CoA synthetase (ACS), plastidic pyruvate dehydrogenase (pPDH), ATP citrate lyase (ACL), Arabidopsis pyruvate decarboxylase (PDC), and Arabidopsis aldehyde dehydrogenase (ALDH), specifically ALDH-2 and ALDH-4. The present invention also provides a recombinant vector comprising a nucleic acid sequence encoding one of the aforementioned enzymes, an antisense sequence thereto or a ribozyme therefor, a cell transformed with such a vector, antibodies to the enzymes, a plant cell, a plant tissue, a plant organ or a plant in which the level of an enzyme has been altered, and a method of producing such a plant cell, plant tissue, plant organ or plant. Desirably, alteration of the level of enzyme results in an alteration of the level of acetyl CoA in the plant cell, plant tissue, plant organ or plant. In addition, the present invention provides a recombinant vector comprising an antisense sequence of a nucleic acid sequence encoding pyruvate decarboxylase (PDC), the E1α subunit of pPDH, the E1β subunit of pPDH, the E2 subunit of pPDH, mitochondrial pyruvate dehydrogenase (mtPDH) or aldehyde dehydrogenase (ALDH) or a ribozyme that can cleave an RNA molecule encoding PDC, E1α pPDH, E1β pPDH, E2 pPDH, mtPDH or ALDH.
摘要翻译：本发明提供乙酰辅酶A合成酶（ACS），质体丙酮酸脱氢酶（pPDH），ATP柠檬酸裂解酶（ACL），拟南芥丙酮酸脱羧酶（PDC）和拟南芥醛脱氢酶（ALDH），特别是ALDH- 2和ALDH-4。本发明还提供了一种重组载体，其包含编码上述酶之一的核酸序列，其上的反义序列或其核酶，用该载体转化的细胞，对酶的抗体，植物细胞，植物组织，其中酶的水平已被改变的植物器官或植物，以及生产这种植物细胞，植物组织，植物器官或植物的方法。理想地，酶水平的改变导致植物细胞，植物组织，植物器官或植物中乙酰CoA水平的改变。此外，本发明提供了包含编码丙酮酸脱羧酶（PDC），pPDH的E1alpha亚基，pPDH的E1beta亚基，pPDH的E2亚基，线粒体丙酮酸脱氢酶（mtPDH）的核酸序列的反义序列的重组载体，或醛脱氢酶（ALDH）或可切割编码PDC，E1alpha pPDH，E1beta pPDH，E2pPDH，mtPDH或ALDH的RNA分子的核酶。

4. 发明授权

US5282338A Sealing structure 失效
标题翻译：密封结构
公开(公告)号：US5282338A
公开(公告)日：1994-02-01
申请号：US40572
申请日：1993-03-31
申请人： David J. Oliver
发明人： David J. Oliver
IPC分类号： B60J10/00 , B60J10/08 , B64C1/14 , E06B7/16
CPC分类号： B64C1/14 , B60J10/24 , B60J10/30 , B60J10/80
摘要： A seal (5) suitable for sealing a door (3) to an aircraft fuselage (1) and which also acts as a rain channel when the door (3) is open. In one embodiment the seal (5) is of unitary construction and made from high tear strength silicone rubber. It comprises a flange region (7) for attachment to the fuselage (1) and a hollow compressible bulb (8) which connects the flange (7) with a pair of sealing lips (9,10). The invention has the advantage of providing an effective seal for doors having uneven inner surfaces.
摘要翻译：一种适于将门（3）密封到飞行器机身（1）的密封件（5），并且当门（3）打开时也用作雨水通道。在一个实施例中，密封件（5）具有整体结构并由高撕裂强度的硅橡胶制成。它包括用于连接到机身（1）的凸缘区域（7）和将凸缘（7）与一对密封唇缘（9,10）连接的中空可压缩泡罩（8）。本发明的优点是为具有不均匀内表面的门提供有效的密封。

5. 发明授权

US5092194A Deployment arrangement 失效
标题翻译：部署安排
公开(公告)号：US5092194A
公开(公告)日：1992-03-03
申请号：US403581
申请日：1989-09-06
申请人： David J. Oliver , William Moore , Colin Payne , Don Ware
发明人： David J. Oliver , William Moore , Colin Payne , Don Ware
IPC分类号： B64D39/04
CPC分类号： B64D39/04 , Y10T74/20402 , Y10T74/20426
摘要： A deployment arangement comprises an inner tube 10 pivoted to ground at `P` and carrying an outer tube 12 for telescoping movement. The inner end of the outer tube 12 carries a collar assembly 14 which is pivotally connected to a drive or radius arm 18 which is pivoted to ground at `Q`. The radius arm 18 carries a drive plate 50 which defines a cam track in which a cam follower runs. The mechanism is driven by a ram 20. On actuation of the ram 20, the consequent rotation of a drive plate relative to the collar assembly 14 initially executes a programmed rotation of the outer tube 12 about its longitudinal axis and then locks the outer tube against rotation. Thereafter, the outer tube 12 extends telescopically relative to the inner tube and the inner tube pivots about `P`.

6. 发明授权

US5567082A Fastening arrangements 失效
标题翻译：紧固安排
公开(公告)号：US5567082A
公开(公告)日：1996-10-22
申请号：US339154
申请日：1994-11-10
申请人： Craig E. Ball , David J. Oliver
发明人： Craig E. Ball , David J. Oliver
IPC分类号： F16B5/02 , F16B37/04 , F16B21/00
CPC分类号： F16B5/025 , F16B37/04 , Y10T403/142 , Y10T403/16 , Y10T403/1666
摘要： A fastening arrangement for connecting two items comprises a stud 16 secured to one item 14 and a captive fastener 18 located in a fastener body 20 secured to the other item. The captive fastener 18 is made up of a nut 32, a washer 34 and an outer clip 36 for securing the nut to the clip. The nut, washer and clip 36 may all be passed though the access opening 30 in the fastener body when separate, but when assembled, they resist withdrawal.
摘要翻译：用于连接两个物品的紧固装置包括固定到一个物品14的钉16和位于固定到另一个物品上的紧固件主体20中的固定紧固件18。固定紧固件18由螺母32，垫圈34和外夹36构成，用于将螺母固定到夹子上。螺母，垫圈和夹子36可以全部通过分离的紧固件主体中的进入开口30，但是当组装时，它们抵抗抽出。

7. 发明授权

US5304021A Screw threaded locking bolt assembly 失效
标题翻译：螺纹锁紧螺栓总成
公开(公告)号：US5304021A
公开(公告)日：1994-04-19
申请号：US956858
申请日：1992-10-02
申请人： David J. Oliver , David P. Halliday
发明人： David J. Oliver , David P. Halliday
IPC分类号： F16B39/02 , F16B39/10 , F16B39/24
CPC分类号： F16B39/02
摘要： Locking bolt assembly comprises a bush (9) fixed in use with respect to a structure to which a bolt of the assembly is to be engaged and a socket (1) carried on the bush (9) and provided with locking means (6) for preventing rotation of the socket (1) on the bush (9) but permitting limited longitudinal movement thereon. The bolt is threaded and has a head (3) dimensioned for rotational locking engagement within socket (1). A coil spring (7) urges the head (3) and socket (1) into rotational locking engagement. In use the socket (1) may be moved longitudinally with respect to said bush (9) against the action of the spring (7) from a position in which the head (3) and the socket (1) are radically locked, to a position in which the head (3) is freed for rotation and engagement with a co-operating nut fixed to the structure.
摘要翻译：锁定螺栓组件包括相对于组件的螺栓将被接合的结构固定在使用中的衬套（9）和承载在衬套（9）上的插座（1）并且设置有锁定装置（6），用于防止插座（1）旋转到衬套（9）上，但允许其上的纵向移动受限。螺栓是螺纹的并且具有用于在插座（1）内旋转锁定接合的头部（3）。螺旋弹簧（7）促使头部（3）和插座（1）旋转锁定接合。在使用中，插座（1）可以克服弹簧（7）从头部（3）和插座（1）被自由锁定的位置相对于所述衬套（9）纵向移动到头部（3）被释放以与固定到结构的辅助螺母旋转和接合的位置。

8. 外观设计

USD327038S Remotely-controlled security vehicle 失效
公开(公告)号：USD327038S
公开(公告)日：1992-06-16
申请号：US514519
申请日：1990-04-25
申请人： David J. Oliver
设计人： David J. Oliver

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