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1. 发明授权

US09371556B2 Solutions, methods and kits for deactivating nucleic acids 有权
标题翻译：用于灭活核酸的解决方案，方法和试剂盒
公开(公告)号：US09371556B2
公开(公告)日：2016-06-21
申请号：US11073085
申请日：2005-03-04
申请人： Norman C. Nelson , Kenneth A. Browne , Lizhong Dai , James Russell , Mark E. Filipowsky , Margarita B. Kaminsky , Daniel L. Kacian
发明人： Norman C. Nelson , Kenneth A. Browne , Lizhong Dai , James Russell , Mark E. Filipowsky , Margarita B. Kaminsky , Daniel L. Kacian
IPC分类号： C12Q1/68 , A61L2/18 , C11D3/395
CPC分类号： C12Q1/68 , A61L2/186 , C11D3/3956 , C12Q1/6848
摘要： The present invention relates to reagents for use in deactivating nucleic acids and methods of making and using the same.
摘要翻译：本发明涉及用于失活核酸的试剂及其制备和使用方法。

2. 发明授权

US08765652B2 Method of making a formulation for deactivating nucleic acids 有权
标题翻译：制备抑制核酸的制剂的方法
公开(公告)号：US08765652B2
公开(公告)日：2014-07-01
申请号：US13531924
申请日：2012-06-25
申请人： Norman C. Nelson , Kenneth A. Browne , Lizhong Dai , James Russell , Mark E. Filipowsky , Margarita B. Kaminsky , Daniel L. Kacian
发明人： Norman C. Nelson , Kenneth A. Browne , Lizhong Dai , James Russell , Mark E. Filipowsky , Margarita B. Kaminsky , Daniel L. Kacian
IPC分类号： C11D3/20 , C11D3/39 , C09K3/00
CPC分类号： C12Q1/68 , A61L2/186 , C11D3/3956 , C12Q1/6848
摘要： The present invention relates to reagents for use in deactivating nucleic acids and methods of making and using the same.
摘要翻译：本发明涉及用于失活核酸的试剂及其制备和使用方法。

3. 发明授权

US08221705B2 Receptacles for storing substances in different physical states 有权
标题翻译：在不同物理状态下储存物质的容器
公开(公告)号：US08221705B2
公开(公告)日：2012-07-17
申请号：US12337108
申请日：2008-12-17
申请人： Scott S. Breidenthal , Daniel L. Kacian , Richard S. Lee , Norman C. Nelson , Matthew J. Scott , Jason A. Taylor
发明人： Scott S. Breidenthal , Daniel L. Kacian , Richard S. Lee , Norman C. Nelson , Matthew J. Scott , Jason A. Taylor
IPC分类号： G01N33/00
CPC分类号： B01F11/00 , B01F3/1271 , B01F11/0045 , B01F11/0071 , B01F13/0059 , B01F15/00214 , B01F2215/0037 , B01L3/502 , B01L3/502715 , B01L3/50273 , B01L3/502761 , B01L7/52 , B01L7/525 , B01L2200/0621 , B01L2200/0647 , B01L2200/0673 , B01L2200/0689 , B01L2200/10 , B01L2200/14 , B01L2200/16 , B01L2300/0816 , B01L2300/087 , B01L2300/0887 , B01L2300/123 , B01L2300/1805 , B01L2300/1822 , B01L2300/1883 , B01L2400/0457 , B01L2400/0478 , B01L2400/0481 , B01L2400/0655 , B01L2400/0677 , C12N15/1006 , C12Q1/6806 , C12Q1/6844 , G01N1/44 , G01N21/01 , G01N21/6428 , G01N21/645 , G01N35/0098 , G01N2021/6419 , G01N2021/6421 , G01N2021/6432 , G01N2021/6439 , G01N2021/6463 , G01N2035/00544 , G01N2201/0627 , Y10T137/0391 , Y10T436/143333 , Y10T436/25 , Y10T436/255
摘要： A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte present in a sample. The detector can be used to detect an optical signal emitted by the contents of the receptacle.
摘要翻译：具有多个互连室的容器，其布置成允许独立地或同时地执行多个工艺步骤或工艺。制造容器以将液体与干燥试剂分开并保持干燥试剂的稳定性。包括不混溶的液体，如油，以控制加工材料的加载，促进干燥试剂的混合和重构，限制蒸发，控制反应材料的加热，浓缩固体支持材料以防止流体连接堵塞，为流体转移，并防止工艺材料粘附到室表面。插座可以适用于具有处理器具的系统，该处理器具包括用于选择性地移动腔室和检测器之间的流体物质的致动器系统。致动器系统可以被布置成集中存在于样品中的分析物。检测器可用于检测由容器的内容物发射的光信号。

4. 发明申请

US20090139992A1 RECEPTACLES FOR STORING SUBSTANCES IN DIFFERENT PHYSICAL STATES 有权
标题翻译：用于在不同物理状态储存物质的容器
公开(公告)号：US20090139992A1
公开(公告)日：2009-06-04
申请号：US12337108
申请日：2008-12-17
申请人： Scott S. Breidenthal , Daniel L. Kacian , Richard S. Lee , Norman C. Nelson , Matthew J. Scott , Jason A. Taylor
发明人： Scott S. Breidenthal , Daniel L. Kacian , Richard S. Lee , Norman C. Nelson , Matthew J. Scott , Jason A. Taylor
IPC分类号： B65D25/04
CPC分类号： B01F11/00 , B01F3/1271 , B01F11/0045 , B01F11/0071 , B01F13/0059 , B01F15/00214 , B01F2215/0037 , B01L3/502 , B01L3/502715 , B01L3/50273 , B01L3/502761 , B01L7/52 , B01L7/525 , B01L2200/0621 , B01L2200/0647 , B01L2200/0673 , B01L2200/0689 , B01L2200/10 , B01L2200/14 , B01L2200/16 , B01L2300/0816 , B01L2300/087 , B01L2300/0887 , B01L2300/123 , B01L2300/1805 , B01L2300/1822 , B01L2300/1883 , B01L2400/0457 , B01L2400/0478 , B01L2400/0481 , B01L2400/0655 , B01L2400/0677 , C12N15/1006 , C12Q1/6806 , C12Q1/6844 , G01N1/44 , G01N21/01 , G01N21/6428 , G01N21/645 , G01N35/0098 , G01N2021/6419 , G01N2021/6421 , G01N2021/6432 , G01N2021/6439 , G01N2021/6463 , G01N2035/00544 , G01N2201/0627 , Y10T137/0391 , Y10T436/143333 , Y10T436/25 , Y10T436/255
摘要： A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte present in a sample. The detector can be used to detect an optical signal emitted by the contents of the receptacle.
摘要翻译：具有多个互连室的容器，其布置成允许独立地或同时地执行多个工艺步骤或工艺。制造容器以将液体与干燥试剂分开并保持干燥试剂的稳定性。包括不混溶的液体，如油，以控制加工材料的加载，促进干燥试剂的混合和重构，限制蒸发，控制反应材料的加热，浓缩固体支持材料以防止流体连接堵塞，为流体转移，并防止工艺材料粘附到室表面。插座可以适用于具有处理器具的系统，该处理器具包括用于选择性地移动腔室和检测器之间的流体物质的致动器系统。致动器系统可以被布置成集中存在于样品中的分析物。检测器可用于检测由容器的内容物发射的光信号。

5. 发明授权

US5824475A Oligonucleotide screening assay using DNA-altering agents and probes with protectable labels 失效
标题翻译：使用具有可保护标签的DNA改变剂和探针的寡核苷酸筛选测定
公开(公告)号：US5824475A
公开(公告)日：1998-10-20
申请号：US480884
申请日：1995-06-07
申请人： Norman C. Nelson , Jorge Velarde, Jr. , Daniel L. Kacian
发明人： Norman C. Nelson , Jorge Velarde, Jr. , Daniel L. Kacian
IPC分类号： C12N15/09 , C12Q1/68
CPC分类号： C12Q1/6816 , Y10T436/143333
摘要： The invention features a method for assaying the ability of an oligonucleotide to form a hybrid with a target nucleic acid sequence. The featured assay comprises the following steps: (a) contacting a test sample containing a target nucleic acid sequence with an oligonucleotide; (b) treating the test sample with a duplex-altering agent; (c) contacting the treated test sample with a labeled probe under stringent hybridization conditions, wherein the probe can hybridize to non-altered target nucleic acid sequence such that the probe label is protected from subsequent chemical inactivation but the probe cannot hybridize to altered target nucleic acid sequence in a manner which would protect the probe label from subsequent chemical inactivation; and (d) measuring the hybridization of the probe to non-altered target nucleic acid sequence by measuring the amount of protected label. Also described is an assay to measure the ability of a ribozyme to cleave a target nucleic acid sequence, and an assay to measure the ability of an agent to alter nucleic acid.
摘要翻译：本发明的特征在于测定寡核苷酸与靶核酸序列形成杂交体的能力的方法。特征测定包括以下步骤：（a）将含有靶核酸序列的测试样品与寡核苷酸接触; （b）用双相改变剂处理测试样品; （c）在严格杂交条件下使经处理的测试样品与标记的探针接触，其中所述探针可以与未改变的靶核酸序列杂交，使得探针标记物免受随后的化学失活的影响，但探针不能与改变的靶核酸杂交酸序列，以保护探针标签免受随后的化学灭活; 和（d）通过测量受保护标记的量来测量探针与未改变的靶核酸序列的杂交。还描述了测量核酶切割靶核酸序列的能力的测定法，以及测定试剂改变核酸能力的测定法。

6. 发明授权

US09169512B2 Methods and compositions for nucleic acid amplification 有权
标题翻译：用于核酸扩增的方法和组合物
公开(公告)号：US09169512B2
公开(公告)日：2015-10-27
申请号：US13380018
申请日：2010-07-01
申请人： Steven T. Brentano , Dmitry Lyakhov , James D. Carlson , Michael M. Becker , Norman C. Nelson , Lyle J. Arnold, Jr.
发明人： Steven T. Brentano , Dmitry Lyakhov , James D. Carlson , Michael M. Becker , Norman C. Nelson , Lyle J. Arnold, Jr.
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6865 , C12Q1/6844 , C12Q1/6848 , C12Q1/6853 , C12Q2549/119 , C12Q2537/143 , C12Q2531/143
摘要： Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.
摘要翻译：用于进行扩增反应的组合物，反应混合物和方法，包括多重扩增反应，其中所述方法包括使用包含连接的第一和第二扩增寡聚体成员的一种或多种扩增寡聚体复合物。一方面，将扩增寡聚物复合物与靶核酸杂交，然后捕获具有杂交扩增寡聚物复合物的靶核酸，并洗涤其它组分。靶核酸的靶序列被预扩增以产生第一扩增产物。第一扩增产物在一个或多个二级扩增反应中扩增以产生第二扩增产物。

7. 发明授权

US08642268B2 Methods and compositions for nucleic acid amplification 有权
标题翻译：用于核酸扩增的方法和组合物
公开(公告)号：US08642268B2
公开(公告)日：2014-02-04
申请号：US13460341
申请日：2012-04-30
申请人： Steven T. Brentano , Dmitry Lyakhov , James D. Carlson , Norman C. Nelson , Lyle J. Arnold, Jr. , Michael M. Becker
发明人： Steven T. Brentano , Dmitry Lyakhov , James D. Carlson , Norman C. Nelson , Lyle J. Arnold, Jr. , Michael M. Becker
IPC分类号： C12Q1/68 , C12P19/34 , C07H19/00
CPC分类号： C12Q1/6881 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q2525/155 , C12Q2525/186 , C12Q2525/197 , C12Q2531/143 , C12Q2565/543
摘要： Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.
摘要翻译：公开了用于体外核酸扩增的组合物，其包括目标特异性通用（TSU）启动子引物或启动子提供者寡核苷酸，其包括与扩增的靶序列特异性杂交的靶特异性（TS）序列和通用（U）序列，通过使用通用序列的引物引入扩增的序列。公开了体外核酸扩增方法，其中使用一种或多种TSU寡核苷酸将U序列连接到目标捕获步骤中的靶核酸，然后在基本上等温条件下进行的随后扩增步骤中使用U序列引物制备含有指示样品中靶核酸存在的U序列的扩增产物。

8. 发明授权

US08368882B2 Systems and methods for detecting a signal and applying thermal energy to a signal transmission element 有权
标题翻译：用于检测信号并向信号传输元件施加热能的系统和方法
公开(公告)号：US08368882B2
公开(公告)日：2013-02-05
申请号：US12696406
申请日：2010-01-29
申请人： David Opalsky , George T. Walker , Norman C. Nelson , Richard S. Lee , Sara H. Fan
发明人： David Opalsky , George T. Walker , Norman C. Nelson , Richard S. Lee , Sara H. Fan
IPC分类号： G01N21/01
CPC分类号： G01N21/03 , B01L3/5027 , B01L3/505 , B01L7/52 , B01L9/527 , B01L2200/04 , B01L2200/146 , B01L2300/021 , B01L2300/044 , B01L2300/0654 , B01L2300/0672 , B01L2300/0816 , B01L2300/087 , B01L2300/1827 , B01L2400/043 , B01L2400/0478 , B01L2400/0481 , B01L2400/0487 , B01L2400/0605 , B01L2400/0666 , G01N21/0332 , G01N2021/0328 , G01N2021/0346 , G01N2035/00346
摘要： A signal detection system configured for detecting a signal emitted by the contents of a reaction receptacle is also configured to apply thermal energy to a portion of the reaction receptacle to affect a reaction occurring within the reaction receptacle. More particularly, a system for detecting electromagnetic radiation emitted by the contents of a reaction receptacle includes a transmission element configured for transmitting electromagnetic radiation from the contents of the receptacle, a thermal element associated with the transmission element and configured to apply thermal energy to at least a portion of the receptacle, and a detector configured to receive electromagnetic radiation from the transmission element and to generate a signal corresponding to a characteristic of the electromagnetic radiation received by the detector.
摘要翻译：被配置为检测由反应容器的内容物发射的信号的信号检测系统还被配置为将热能施加到反应容器的一部分以影响在反应容器内发生的反应。更具体地，用于检测由反应容器的内容物发射的电磁辐射的系统包括传输元件，其被配置用于从容器的内容物传输电磁辐射，与传输元件相关联的热元件，并被配置为将热能施加到至少所述插座的一部分以及被配置为从所述传输元件接收电磁辐射并且产生对应于由所述检测器接收的电磁辐射的特性的信号的检测器。

9. 发明申请

US20090142771A1 Methods and Instruments for Processing a Sample in a Multi-Chambered Receptacle 有权
标题翻译：用于在多声道插座中处理样品的方法和仪器
公开(公告)号：US20090142771A1
公开(公告)日：2009-06-04
申请号：US12333334
申请日：2008-12-12
申请人： Scott S. Breidenthal , Sara H. Fan , Richard S. Lee , Norman C. Nelson , Matthew J. Scott , Jason A. Taylor
发明人： Scott S. Breidenthal , Sara H. Fan , Richard S. Lee , Norman C. Nelson , Matthew J. Scott , Jason A. Taylor
IPC分类号： C12Q1/68 , G01N33/00 , C12M1/00
CPC分类号： B01F11/00 , B01F3/1271 , B01F11/0045 , B01F11/0071 , B01F13/0059 , B01F15/00214 , B01F2215/0037 , B01L3/502 , B01L3/502715 , B01L3/50273 , B01L3/502761 , B01L7/52 , B01L7/525 , B01L2200/0621 , B01L2200/0647 , B01L2200/0673 , B01L2200/0689 , B01L2200/10 , B01L2200/14 , B01L2200/16 , B01L2300/0816 , B01L2300/087 , B01L2300/0887 , B01L2300/123 , B01L2300/1805 , B01L2300/1822 , B01L2300/1883 , B01L2400/0457 , B01L2400/0478 , B01L2400/0481 , B01L2400/0655 , B01L2400/0677 , C12N15/1006 , C12Q1/6806 , C12Q1/6844 , G01N1/44 , G01N21/01 , G01N21/6428 , G01N21/645 , G01N35/0098 , G01N2021/6419 , G01N2021/6421 , G01N2021/6432 , G01N2021/6439 , G01N2021/6463 , G01N2035/00544 , G01N2201/0627 , Y10T137/0391 , Y10T436/143333 , Y10T436/25 , Y10T436/255
摘要： A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte present in a sample. The detector can be used to detect an optical signal emitted by the contents of the receptacle.
摘要翻译：具有多个互连室的容器，其布置成允许独立地或同时地执行多个工艺步骤或工艺。制造容器以将液体与干燥试剂分开并保持干燥试剂的稳定性。包括不混溶的液体，如油，以控制加工材料的加载，促进干燥试剂的混合和重构，限制蒸发，控制反应材料的加热，浓缩固体支持材料以防止流体连接堵塞，为流体转移，并防止工艺材料粘附到室表面。插座可以适用于具有处理器具的系统，该处理器具包括用于选择性地移动腔室和检测器之间的流体物质的致动器系统。致动器系统可以被布置成集中存在于样品中的分析物。检测器可用于检测由容器的内容物发射的光信号。

10. 发明授权

US5639604A Homogeneous protection assay 失效
标题翻译：均质保护试验
公开(公告)号：US5639604A
公开(公告)日：1997-06-17
申请号：US161706
申请日：1993-12-03
申请人： Lyle J. Arnold, Jr. , Norman C. Nelson
发明人： Lyle J. Arnold, Jr. , Norman C. Nelson
IPC分类号： C12Q1/68 , G01N33/53 , G01N33/532 , G01N33/533 , G01N33/542 , G01N33/58 , C07H21/04 , C12Q1/00
CPC分类号： G01N33/58 , C12Q1/6816 , C12Q1/6823 , G01N33/5306 , G01N33/532 , G01N33/533 , G01N33/542
摘要： Improved homogenous diagnostic assay methods and labels for detecting an analyte in a medium when the analyte is a member of a specific binding pair. The methods and labels provide procedures for reducing background and increasing sensitivity. The binding partner of the analyte is labeled with a substance, the stability of which detectably changes whenever said analyte is bound as a member of the specific binding pair. In a closely related system, the analyte is labeled with a substance susceptible to differential degradation depending on whether or not the analyte is bound as a member of its specific binding pair. After incubation and selective degradation or chemical or biochemical alteration, the amount of analyte bound is detected by measuring either the stability change or the extent of degradation of the label. In a particular system, chemiluminescent acridinium ester labeled probes are used in a homogenous hybridization assay format for sensitively detecting the presence of complement any target polynucleotide sequences.
摘要翻译：当分析物是特异性结合对的成员时，改进的同源诊断测定方法和用于检测培养基中分析物的标记。方法和标签提供了降低背景和提高灵敏度的程序。分析物的结合配偶体用物质标记，当所述分析物作为特异性结合对的成员结合时，其稳定性可检测地改变。在紧密相关的系统中，根据分析物是否作为其特异性结合对的成员结合，分析物被易受差异降解敏感的物质标记。孵育和选择性降解或化学或生物化学改变后，通过测量标签的稳定性变化或降解程度来检测分析物的结合量。在特定系统中，化学发光的吖啶酯标记的探针以均质杂交测定形式使用，以敏感地检测补体存在任何靶多核苷酸序列。

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