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1. 发明授权

US08846309B2 Methods for detecting Lp-PLA2 activity and inhibition of Lp-PLA2 activity 有权
标题翻译：检测Lp-PLA2活性和抑制Lp-PLA2活性的方法
公开(公告)号：US08846309B2
公开(公告)日：2014-09-30
申请号：US12817677
申请日：2010-06-17
申请人： Yaping Shou , Yin-Fai Siu , George T. Walker
发明人： Yaping Shou , Yin-Fai Siu , George T. Walker
IPC分类号： C12Q1/00 , C12Q1/61 , C12Q1/34 , C12Q1/44
CPC分类号： C12Q1/61 , C12Q1/34 , C12Q1/44 , C12Q2334/10 , G01N2333/918
摘要： This invention relates to methods for determining the activity of Lp-PLA2 in at least one sample from an animal. The invention also relates to methods for determining the inhibition of Lp-PLA2 activity in samples from animals that are administered an Lp-PLA2 inhibitor.
摘要翻译：本发明涉及用于测定来自动物的至少一种样品中Lp-PLA2的活性的方法。本发明还涉及用于测定来自施用Lp-PLA2抑制剂的动物的样品中Lp-PLA2活性的抑制的方法。

2. 发明申请

US20100256919A1 METHODS FOR DETECTING LP-PLA2 ACTIVITY AND INHIBITION OF LP-PLA2 ACTIVITY 有权
标题翻译：检测LP-PLA2活性和抑制LP-PLA2活性的方法
公开(公告)号：US20100256919A1
公开(公告)日：2010-10-07
申请号：US12817677
申请日：2010-06-17
申请人： Yaping Shou , Yin-Fai Siu , George T. Walker
发明人： Yaping Shou , Yin-Fai Siu , George T. Walker
IPC分类号： G01N33/53 , G06F19/00
CPC分类号： C12Q1/61 , C12Q1/34 , C12Q1/44 , C12Q2334/10 , G01N2333/918
摘要： This invention relates to methods for determining the activity of Lp-PLA2 in at least one sample from an animal. The invention also relates to methods for determining the inhibition of Lp-PLA2 activity in samples from animals that are administered an Lp-PLA2 inhibitor.
摘要翻译：本发明涉及用于测定来自动物的至少一种样品中Lp-PLA2的活性的方法。本发明还涉及用于测定来自施用Lp-PLA2抑制剂的动物的样品中Lp-PLA2活性的抑制的方法。

3. 发明授权

US5624825A Simultaneous amplification of multiple targets 失效
标题翻译：同时放大多个目标
公开(公告)号：US5624825A
公开(公告)日：1997-04-29
申请号：US451313
申请日：1995-05-26
申请人： George T. Walker , James G. Nadeau , Michael C. Little
发明人： George T. Walker , James G. Nadeau , Michael C. Little
IPC分类号： G01N33/566 , C07H21/04 , C12N15/09 , C12P19/34 , C12Q1/68 , G11B5/00 , G11B5/09 , G11B5/187 , G11B5/31 , G11B5/455 , G11B5/465 , G11B19/04
CPC分类号： C12Q1/6853
摘要： Methods for multiplex amplification of target nucleic acid sequences using a single pair of primers. Defined sequences are appended to the ends of multiple target sequences as part of the amplification reaction so that no steps in addition to amplification are required. The target sequences with the appended defined sequences need not be isolated prior to amplification. In one embodiment for coamplification of two target sequences, a sequence corresponding to a terminal segment of the first target sequence is appended to one end of the second target sequence and a sequence corresponding to a terminal segment of the second target sequence is appended to one end of the first target sequence. Amplification of the two targets then requires only a single pair of primers. Alternatively, a single defined sequence may be appended to the 5' and 3' ends of any number of selected targets. All such modified target sequences may then be amplified using a single pair of primers which hybridize to the defined end-sequences.
摘要翻译：使用单对引物多重扩增靶核酸序列的方法。定义的序列作为扩增反应的一部分附加到多个靶序列的末端，因此不需要扩增步骤。具有附加定义序列的靶序列不需要在扩增前分离。在两个目标序列的共放大的一个实施例中，对应于第一目标序列的末端片段的序列被附加到第二目标序列的一端，并且与第二目标序列的末端片段相对应的序列被附加到一端的第一个靶序列。两个靶标的扩增只需要一对引物即可。或者，单个定义的序列可以附加到任意数目的所选目标的5'和3'端。然后可以使用与定义的末端序列杂交的单对引物扩增所有这些修饰的靶序列。

4. 发明授权

US5270184A Nucleic acid target generation 失效
标题翻译：核酸靶产生
公开(公告)号：US5270184A
公开(公告)日：1993-12-14
申请号：US794399
申请日：1991-11-19
申请人： George T. Walker , Michael C. Little , James G. Nadeau
发明人： George T. Walker , Michael C. Little , James G. Nadeau
IPC分类号： C12N15/02 , C12N15/09 , C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6846 , C12Q1/6853
摘要： The invention is a method for generating nucleic acid sequences ends which comprises;(a) hybridizing a primer to a nucleic acid sequence,(b) hybridizing a primer to the nucleic acid sequence in (a) located 5' to the primer in (a), and(c) polymerizing both primers so that the primer in (a) is displaced from the nucleic acid sequence.The invention provides a method for generating target nucleic acid sequences for subsequent amplification. The method is applicable to both DNA and RNA.

5. 发明申请

US20150017671A1 METHODS FOR DETECTING LP-PLA2 ACTIVITY AND INHIBITION OF LP-PLA2 ACTIVITY 审中-公开
公开(公告)号：US20150017671A1
公开(公告)日：2015-01-15
申请号：US14498980
申请日：2014-09-26
申请人： Yaping SHOU , Yin-Fai SIU , George T. WALKER
发明人： Yaping SHOU , Yin-Fai SIU , George T. WALKER
IPC分类号： C12Q1/44
CPC分类号： G01N33/573 , C12Q1/44 , C12Q2334/10 , G01N33/581 , G01N2333/918 , G01N2500/02 , G01N2500/04 , G01N2500/20
摘要： This invention relates to methods for determining the activity of Lp-PLA2 in at least one sample from an animal. The invention also relates to methods for determining the inhibition of Lp-PLA2 activity in samples from animals that are administered an Lp-PLA2 inhibitor.

6. 发明授权

US08609357B2 Methods for detecting LP-PLA2 activity and inhibition of LP-PLA2 activity 有权
标题翻译：检测LP-PLA2活性和抑制LP-PLA2活性的方法
公开(公告)号：US08609357B2
公开(公告)日：2013-12-17
申请号：US13540571
申请日：2012-07-02
申请人： Yaping Shou , Yin-Fai Siu , George T. Walker
发明人： Yaping Shou , Yin-Fai Siu , George T. Walker
IPC分类号： C12Q1/44 , C12Q1/00
CPC分类号： C12Q1/61 , C12Q1/34 , C12Q1/44 , C12Q2334/10 , G01N2333/918
摘要： This invention relates to methods for determining the activity of Lp-PLA2 in at least one sample from an animal. The invention also relates to methods for determining the inhibition of Lp-PLA2 activity in samples from animals that are administered an Lp-PLA2 inhibitor.
摘要翻译：本发明涉及用于测定来自动物的至少一种样品中Lp-PLA2的活性的方法。本发明还涉及用于测定来自施用Lp-PLA2抑制剂的动物的样品中Lp-PLA2活性的抑制的方法。

7. 发明授权

US08368882B2 Systems and methods for detecting a signal and applying thermal energy to a signal transmission element 有权
标题翻译：用于检测信号并向信号传输元件施加热能的系统和方法
公开(公告)号：US08368882B2
公开(公告)日：2013-02-05
申请号：US12696406
申请日：2010-01-29
申请人： David Opalsky , George T. Walker , Norman C. Nelson , Richard S. Lee , Sara H. Fan
发明人： David Opalsky , George T. Walker , Norman C. Nelson , Richard S. Lee , Sara H. Fan
IPC分类号： G01N21/01
CPC分类号： G01N21/03 , B01L3/5027 , B01L3/505 , B01L7/52 , B01L9/527 , B01L2200/04 , B01L2200/146 , B01L2300/021 , B01L2300/044 , B01L2300/0654 , B01L2300/0672 , B01L2300/0816 , B01L2300/087 , B01L2300/1827 , B01L2400/043 , B01L2400/0478 , B01L2400/0481 , B01L2400/0487 , B01L2400/0605 , B01L2400/0666 , G01N21/0332 , G01N2021/0328 , G01N2021/0346 , G01N2035/00346
摘要： A signal detection system configured for detecting a signal emitted by the contents of a reaction receptacle is also configured to apply thermal energy to a portion of the reaction receptacle to affect a reaction occurring within the reaction receptacle. More particularly, a system for detecting electromagnetic radiation emitted by the contents of a reaction receptacle includes a transmission element configured for transmitting electromagnetic radiation from the contents of the receptacle, a thermal element associated with the transmission element and configured to apply thermal energy to at least a portion of the receptacle, and a detector configured to receive electromagnetic radiation from the transmission element and to generate a signal corresponding to a characteristic of the electromagnetic radiation received by the detector.
摘要翻译：被配置为检测由反应容器的内容物发射的信号的信号检测系统还被配置为将热能施加到反应容器的一部分以影响在反应容器内发生的反应。更具体地，用于检测由反应容器的内容物发射的电磁辐射的系统包括传输元件，其被配置用于从容器的内容物传输电磁辐射，与传输元件相关联的热元件，并被配置为将热能施加到至少所述插座的一部分以及被配置为从所述传输元件接收电磁辐射并且产生对应于由所述检测器接收的电磁辐射的特性的信号的检测器。

8. 发明授权

US5547861A Detection of nucleic acid amplification 失效
标题翻译：检测核酸扩增
公开(公告)号：US5547861A
公开(公告)日：1996-08-20
申请号：US229281
申请日：1994-04-18
申请人： James G. Nadeau , George T. Walker
发明人： James G. Nadeau , George T. Walker
IPC分类号： C12N15/09 , C12Q1/68 , C12R1/32 , C12P19/34 , C07H21/04 , C12Q1/70
CPC分类号： C12Q1/6844
摘要： Methods for detecting, immobilizing or localizing primer extension products of a Strand Displacement Amplification reaction which are coupled to, and an indication of, amplification of the target sequence. The primer extension products are secondary, target-specific DNA products generated concurrently with SDA of the target sequence and can therefore be used to detect and/or measure target sequence amplification in real-time. In general, the secondary amplification products are not amplifiable and remain inert in the SDA reaction after they are formed without interfering with amplification of the target sequence. The secondary amplification products may be designed or modified to contain special features to facilitate their detection, immobilization or localization.
摘要翻译：用于检测，固定或定位连接于链序列扩增反应的引物延伸产物的方法，以及扩增靶序列的指示。引物延伸产物是与靶序列的SDA同时产生的次要靶特异性DNA产物，因此可用于实时检测和/或测量靶序列扩增。通常，二次扩增产物在其形成后不能扩增并在SDA反应中保持惰性，而不干扰靶序列的扩增。二次扩增产物可以被设计或修改以包含特殊特征以促进其检测，固定或定位。

9. 发明授权

US5536649A Decontamination of nucleic acid amplification reactions using uracil-N-glycosylase (UDG) 失效
标题翻译：使用尿嘧啶-N-糖基化酶（UDG）进行核酸扩增反应的去污
公开(公告)号：US5536649A
公开(公告)日：1996-07-16
申请号：US283117
申请日：1994-07-29
申请人： Melinda S. Fraiser , George T. Walker , James L. Schram
发明人： Melinda S. Fraiser , George T. Walker , James L. Schram
IPC分类号： C12N15/09 , C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6844 , C12Q1/6848
摘要： Methods for inactivating contaminating amplicons in isothermal nucleic acid amplification reactions such as SDA. Uracil is incorporated into the amplicons produced by amplification in the place of thymine (T) using novel SDA reaction conditions. If these amplicons contaminate a subsequent amplification reaction, they may be inactivated as templates (i.e., rendered unamplifiable) by treatment with UDG. As isothermal amplification does not involve elevated temperatures, the UDG may be inactivated during the subsequent amplification of specific target sequences by inclusion of the UDG inhibitor protein Ugi. Incorporation of dU has unexpectedly been found to enhance the amplification power of SDA as compared to conventional SDA reactions. The methods may also be used to detect UDG activity in reagents or samples.
摘要翻译：在等温核酸扩增反应（如SDA）中灭活污染扩增子的方法。尿嘧啶通过使用新的SDA反应条件通过扩增代替胸腺嘧啶（T）而产生的扩增子。如果这些扩增子污染随后的扩增反应，则它们可以通过用UDG处理作为模板灭活（即，使其不能扩增）。由于等温扩增不包括升高的温度，所以UDG可以在随后通过包含UDG抑制剂蛋白Ugi扩增特异性靶序列期间失活。与传统的SDA反应相比，意外地发现dU的引入增强了SDA的放大功率。该方法也可用于检测试剂或样品中的UDG活性。

10. 发明授权

US5097925A Tree walker 失效
标题翻译：树步行者
公开(公告)号：US5097925A
公开(公告)日：1992-03-24
申请号：US537636
申请日：1990-06-14
申请人： George T. Walker, Jr.
发明人： George T. Walker, Jr.
IPC分类号： A01M31/02 , A47C9/10 , A63B27/00
CPC分类号： A63B27/00 , A01M31/02
摘要： A lightweight, portable, tree-climbing device to provide a stable, elevated, horizontal, platfrom utilizing two separate elements for a hunter or observer. The uppermost element accommodates the user in a sitting position while the lower element accommodates his feet. The user faces the tree during the ascending and descending processes but has the option of facing the tree or leaning his back against the tree during his hunting or observing activity. Each element has tubular supporting members, which, along the the ends of the supporting cables, have predrilled holes, thus allowing for the selective adjustment of each element during initial attachment. The device further provides for selective adjustment of the support cables as the user ascends and descends the tree. The device further allows the user to climb past limbs without first having to cut them from the tree.
摘要翻译：一种轻便，便携的攀登装置，可以为猎人或观察者提供一个稳定，高架，水平的平台。最上面的元件容纳使用者处于坐姿，而下部元件容纳他的脚。用户在上升和下降过程中面对树，但是可以在狩猎或观察活动期间面对树或将其靠在树上。每个元件具有管状支撑构件，其沿着支撑电缆的端部具有预先加工的孔，从而允许在初始附接期间对每个元件进行选择性调节。该装置还用于当用户上升和下降时选择性地调整支撑电缆。该装置还允许用户爬过四肢，而无需首先将它们从树上切下。

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