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1. 发明申请

US20100142850A1 System, method, and product for scanning of biological materials 有权
标题翻译：用于生物材料扫描的系统，方法和产品
公开(公告)号：US20100142850A1
公开(公告)日：2010-06-10
申请号：US12704350
申请日：2010-02-11
申请人： Nathan K. Weiner , Patrick J. Odoy , Eric Schultz , Mark Jones , James Overbeck , Herman Deweerd , David A. Stura , Albert Bukys , Tim Woolaver , Thomas P. Regan , David Bradbury , Eric Earl McKenzie , Roger DiPaolo , Christopher Miles , Joel Katz , Ksenia Oleink-Ovod
发明人： Nathan K. Weiner , Patrick J. Odoy , Eric Schultz , Mark Jones , James Overbeck , Herman Deweerd , David A. Stura , Albert Bukys , Tim Woolaver , Thomas P. Regan , David Bradbury , Eric Earl McKenzie , Roger DiPaolo , Christopher Miles , Joel Katz , Ksenia Oleink-Ovod
IPC分类号： G06K9/40
CPC分类号： G01N21/6428 , G01N21/253 , G01N21/6452 , G01N2021/6471
摘要： An embodiment of a scanning system is described including optical elements that direct an excitation beam at a probe array, detectors that receive reflected intensity data responsive to the excitation beam, where the reflected intensity data is responsive to a focusing distance between an optical element and the probe array, a transport frame that adjusts the focusing distance in a direction with respect to the probe array, an auto-focuser that determines a best plane of focus based upon characteristics of the reflected intensity data of at least two focusing distances where the detectors further receive pixel intensity values based upon detected emissions from a plurality of probe features disposed on the probe array at the best plane of focus, and an image generator that associates each of the pixel intensity values with at least one image pixel position of a probe array based upon one or more position correction values.
摘要翻译：描述了扫描系统的实施例，其包括将激发光束引导到探针阵列的光学元件，响应于激发光束接收反射光强数据的检测器，其中反射光强度数据响应于光学元件和探针阵列，调整相对于探针阵列的方向上的聚焦距离的传输框架，基于至少两个聚焦距离的反射强度数据的特性确定最佳聚焦距离的自动对焦器，其中检测器进一步基于在最佳焦点平面上设置在探针阵列上的多个探针特征的检测到的发射来接收像素强度值，以及图像生成器，其将每个像素强度值与探针阵列的至少一个图像像素位置相关联一个或多个位置校正值。

2. 发明授权

US07983467B2 System, method, and product for scanning of biological materials 有权
公开(公告)号：US07983467B2
公开(公告)日：2011-07-19
申请号：US12704350
申请日：2010-02-11
申请人： Nathan K. Weiner , Patrick J. Odoy , Eric Schultz , Mark Jones , James Overbeck , Herman Deweerd , David A. Stura , Albert Bukys , Tim Woolaver , Thomas P. Regan , David Bradbury , Eric Earl McKenzie , Roger DiPaolo , Christopher Miles , Joel M. Katz , Ksenia Oleink-Ovod
发明人： Nathan K. Weiner , Patrick J. Odoy , Eric Schultz , Mark Jones , James Overbeck , Herman Deweerd , David A. Stura , Albert Bukys , Tim Woolaver , Thomas P. Regan , David Bradbury , Eric Earl McKenzie , Roger DiPaolo , Christopher Miles , Joel M. Katz , Ksenia Oleink-Ovod
IPC分类号： G06K9/00
CPC分类号： G01N21/6428 , G01N21/253 , G01N21/6452 , G01N2021/6471
摘要： An embodiment of a scanning system is described including optical elements that direct an excitation beam at a probe array, detectors that receive reflected intensity data responsive to the excitation beam, where the reflected intensity data is responsive to a focusing distance between an optical element and the probe array, a transport frame that adjusts the focusing distance in a direction with respect to the probe array, an auto-focuser that determines a best plane of focus based upon characteristics of the reflected intensity data of at least two focusing distances where the detectors further receive pixel intensity values based upon detected emissions from a plurality of probe features disposed on the probe array at the best plane of focus, and an image generator that associates each of the pixel intensity values with at least one image pixel position of a probe array based upon one or more position correction values.

3. 发明授权

US07871812B2 System, method, and product for scanning of biological materials 有权
标题翻译：用于生物材料扫描的系统，方法和产品
公开(公告)号：US07871812B2
公开(公告)日：2011-01-18
申请号：US10975788
申请日：2004-10-27
申请人： Nathan K. Weiner , Patrick J. Odoy , Eric Schultz , Mark Jones , James Overbeck , Herman Deweerd , David A. Stura , Albert Bukys , Tim Woolaver , Thomas P. Regan , David Bradbury , Eric Earl McKenzie , Roger DiPaolo , Christopher Miles , Joel M. Katz , Ksenia Oleink-Ovod
发明人： Nathan K. Weiner , Patrick J. Odoy , Eric Schultz , Mark Jones , James Overbeck , Herman Deweerd , David A. Stura , Albert Bukys , Tim Woolaver , Thomas P. Regan , David Bradbury , Eric Earl McKenzie , Roger DiPaolo , Christopher Miles , Joel M. Katz , Ksenia Oleink-Ovod
IPC分类号： C12M1/34 , C12M3/00
CPC分类号： G01N21/6428 , G01N21/253 , G01N21/6452 , G01N2021/6471
摘要： An embodiment of a scanning system is described including optical elements that direct an excitation beam at a probe array, detectors that receive reflected intensity data responsive to the excitation beam, where the reflected intensity data is responsive to a focusing distance between an optical element and the probe array, a transport frame that adjusts the focusing distance in a direction with respect to the probe array, an auto-focuser that determines a best plane of focus based upon characteristics of the reflected intensity data of at least two focusing distances where the detectors further receive pixel intensity values based upon detected emissions from a plurality of probe features disposed on the probe array at the best plane of focus, and an image generator that associates each of the pixel intensity values with at least one image pixel position of a probe array based upon one or more position correction values.
摘要翻译：描述了扫描系统的实施例，其包括将激发光束引导到探针阵列的光学元件，响应于激发光束接收反射光强数据的检测器，其中反射光强度数据响应于光学元件和探针阵列，调整相对于探针阵列的方向上的聚焦距离的传输框架，基于至少两个聚焦距离的反射强度数据的特性确定最佳聚焦距离的自动对焦器，其中检测器进一步基于在最佳焦点平面上设置在探针阵列上的多个探针特征的检测到的发射来接收像素强度值，以及图像生成器，其将每个像素强度值与探针阵列的至少一个图像像素位置相关联一个或多个位置校正值。

4. 发明授权

US07689022B2 System, method, and product for scanning of biological materials 有权
公开(公告)号：US07689022B2
公开(公告)日：2010-03-30
申请号：US10389194
申请日：2003-03-14
申请人： Nathan K. Weiner , Patrick J. Odoy , Erik Schultz , Mark Jones , James Overbeck , Herman Deweerd , David A. Stura , Albert Bukys , Tim Woolaver , Thomas P. Regan , David Bradbury , Eric Earl McKenzie , Roger DiPaolo , Christopher Miles , Joel Katz , Ksenia Oleink-Ovod
发明人： Nathan K. Weiner , Patrick J. Odoy , Erik Schultz , Mark Jones , James Overbeck , Herman Deweerd , David A. Stura , Albert Bukys , Tim Woolaver , Thomas P. Regan , David Bradbury , Eric Earl McKenzie , Roger DiPaolo , Christopher Miles , Joel Katz , Ksenia Oleink-Ovod
IPC分类号： G06K9/00
CPC分类号： G01N21/6428 , G01N21/253 , G01N21/6452 , G01N2021/6471
摘要： An embodiment of a scanning system is described including optical elements that direct an excitation beam at a probe array, detectors that receive reflected intensity data responsive to the excitation beam, where the reflected intensity data is responsive to a focusing distance between an optical element and the probe array, a transport frame that adjusts the focusing distance in a direction with respect to the probe array, an auto-focuser that determines a best plane of focus based upon characteristics of the reflected intensity data of at least two focusing distances where the detectors further receive pixel intensity values based upon detected emissions from a plurality of probe features disposed on the probe array at the best plane of focus, and an image generator that associates each of the pixel intensity values with at least one image pixel position of a probe array based upon one or more position correction values.

5. 发明申请

US20120235016A1 System, Method, and Product for Scanning of Biological Materials 有权
标题翻译：生物材料扫描的系统，方法和产品
公开(公告)号：US20120235016A1
公开(公告)日：2012-09-20
申请号：US13480888
申请日：2012-05-25
申请人： Nathan K. Weiner , Patrick J. Odoy , Eric Schultz , Mark Jones , James Overbeck , Herman Deweerd , David A. Stura , Albert Bukys , Tim Woolaver , Thomas P. Regan , David Bradbury , Eric Earl McKenzie , Roger DiPaolo , Christopher Miles , Joel Katz , Ksenia Oleinik-Ovod
发明人： Nathan K. Weiner , Patrick J. Odoy , Eric Schultz , Mark Jones , James Overbeck , Herman Deweerd , David A. Stura , Albert Bukys , Tim Woolaver , Thomas P. Regan , David Bradbury , Eric Earl McKenzie , Roger DiPaolo , Christopher Miles , Joel Katz , Ksenia Oleinik-Ovod
IPC分类号： H01L27/146
CPC分类号： G01N21/6428 , G01N21/253 , G01N21/6452 , G01N2021/6471
摘要： An embodiment of a scanning system is described including optical elements that direct an excitation beam at a probe array, detectors that receive reflected intensity data responsive to the excitation beam, where the reflected intensity data is responsive to a focusing distance between an optical element and the probe array, a transport frame that adjusts the focusing distance in a direction with respect to the probe array, an auto-focuser that determines a best plane of focus based upon characteristics of the reflected intensity data of at least two focusing distances where the detectors further receive pixel intensity values based upon detected emissions from a plurality of probe features disposed on the probe array at the best plane of focus, and an image generator that associates each of the pixel intensity values with at least one image pixel position of a probe array based upon one or more position correction values.
摘要翻译：描述了扫描系统的实施例，其包括将激发光束引导到探针阵列的光学元件，响应于激发光束接收反射光强数据的检测器，其中反射光强度数据响应于光学元件和探针阵列，调整相对于探针阵列的方向上的聚焦距离的传输框架，基于至少两个聚焦距离的反射强度数据的特性确定最佳聚焦距离的自动对焦器，其中检测器进一步基于在最佳焦点平面上设置在探针阵列上的多个探针特征的检测到的发射来接收像素强度值，以及图像生成器，其将每个像素强度值与探针阵列的至少一个图像像素位置相关联一个或多个位置校正值。

6. 发明申请

US20050057676A1 System, method, and product for scanning of biological materials 有权
标题翻译：用于生物材料扫描的系统，方法和产品
公开(公告)号：US20050057676A1
公开(公告)日：2005-03-17
申请号：US10975788
申请日：2004-10-27
申请人： Nathan Weiner , Patrick Odoy , Eric Schultz , Mark Jones , James Overbeck , Herman Deweerd , David Stura , Albert Bukys , Tim Woolaver , Thomas Regan , David Bradbury , Eric McKenzie , Roger DiPaolo , Christopher Miles , Joel Katz , Ksenia Oleink-Ovod
发明人： Nathan Weiner , Patrick Odoy , Eric Schultz , Mark Jones , James Overbeck , Herman Deweerd , David Stura , Albert Bukys , Tim Woolaver , Thomas Regan , David Bradbury , Eric McKenzie , Roger DiPaolo , Christopher Miles , Joel Katz , Ksenia Oleink-Ovod
IPC分类号： G01N21/64 , G01N21/25 , G01N33/483 , G01N33/53 , G01N37/00 , G01J3/30
CPC分类号： G01N21/6428 , G01N21/253 , G01N21/6452 , G01N2021/6471
摘要： An embodiment of a scanning system is described including optical elements that direct an excitation beam at a probe array, detectors that receive reflected intensity data responsive to the excitation beam, where the reflected intensity data is responsive to a focusing distance between an optical element and the probe array, a transport frame that adjusts the focusing distance in a direction with respect to the probe array, an auto-focuser that determines a best plane of focus based upon characteristics of the reflected intensity data of at least two focusing distances where the detectors further receive pixel intensity values based upon detected emissions from a plurality of probe features disposed on the probe array at the best plane of focus, and an image generator that associates each of the pixel intensity values with at least one image pixel position of a probe array based upon one or more position correction values.
摘要翻译：描述了扫描系统的实施例，其包括将激发光束引导到探针阵列的光学元件，响应于激发光束接收反射光强数据的检测器，其中反射光强度数据响应于光学元件和探针阵列，调整相对于探针阵列的方向上的聚焦距离的传输框架，基于至少两个聚焦距离的反射强度数据的特性确定最佳聚焦距离的自动对焦器，其中检测器进一步基于在最佳焦点平面上设置在探针阵列上的多个探针特征的检测到的发射来接收像素强度值，以及图像生成器，其将每个像素强度值与探针阵列的至少一个图像像素位置相关联一个或多个位置校正值。

7. 发明申请

US20110319279A1 Assays Based on Liquid Flow Over Arrays 有权
标题翻译：基于液体流过阵列的测定
公开(公告)号：US20110319279A1
公开(公告)日：2011-12-29
申请号：US12876883
申请日：2010-09-07
申请人： Jean I. Montagu , Herman Deweerd , Roger Dowd , Natalia Rodionova , Peter Maimonis , Nathan Tyburczy
发明人： Jean I. Montagu , Herman Deweerd , Roger Dowd , Natalia Rodionova , Peter Maimonis , Nathan Tyburczy
IPC分类号： C40B30/04 , C40B40/10
CPC分类号： B01L3/502746 , B01L3/502715 , B01L3/502723 , B01L3/50273 , B01L3/502738 , B01L7/00 , B01L9/527 , B01L2200/027 , B01L2200/0684 , B01L2200/16 , B01L2300/0636 , B01L2300/0654 , B01L2300/0672 , B01L2300/0816 , B01L2300/0867 , B01L2300/0877 , B01L2300/1827 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655 , B01L2400/0683 , B01L2400/0688 , Y10T436/117497
摘要： Cassette (50) performs assays, e.g. multiplexed protein biomarker assays. Wide, bubble-free, slow flows are produced from liquids stored on cassette (50), flowing over wide array (20) of ligand receptors on a capture surface. Flows of Reynolds Number less than about 1, preferably 1×10−1 to 5×10−3, are heated in region (34) preceding and including bubble removal system (128). Analyte is introduced through compressed septum (32). External actuations of displacement pumps (30, 37) and valves (137 A, B, and C) produce flows in response to flow-front optical sensors (150, 152). Elastic sheet provides pump and valve diaphragms and resilient expansion of mixing volume (131). Break-away cover portions are pistons. Heating is by conduction through cassette from external contact heater. Planar cassette body, when tilted from horizontal, enables upward flow from pumped storage (134, 135) to reaction (133) to waste (139), with buoyancy bubble removal before reaction. Reading of fluorescence is by external reader, employing calibration, control and reference features on capture surface. Extensive set of calibration features of differing intensities enables self-calibration.
摘要翻译：盒（50）进行测定。多重蛋白生物标志物测定。从储存在盒（50）上的液体，在捕获表面上的宽阵列（20）的配体受体上流动，产生宽而无气泡的慢流。小于约1，优选1×10-1至5×10-3的雷诺数在包括气泡去除系统（128）之前的区域（34）中被加热。通过压缩隔膜（32）引入分析物。位移泵（30,37）和阀（137A，B和C）的外部致动响应于前后光学传感器（150,152）产生流动。弹性片提供泵和阀膜片和弹性膨胀的混合量（131）。分离盖部分是活塞。加热是通过外部接触加热器的磁带传导。当从水平方向倾斜时，平面盒体能够在反应之前从泵送储存（134,135）到反应（133）到废物（139）的向上流动。通过外部读取器读取荧光，在捕获表面采用校准，控制和参考特征。广泛的不同强度的校准特征可以进行自校准。

8. 发明申请

US20110207621A1 Assays Based on Liquid Flow over Arrays 审中-公开
标题翻译：基于液体流过阵列的测定
公开(公告)号：US20110207621A1
公开(公告)日：2011-08-25
申请号：US12918757
申请日：2009-02-20
申请人： Jean I. Montagu , Herman Deweerd , Natalia A. Rodionova , Nathan Tyburczy
发明人： Jean I. Montagu , Herman Deweerd , Natalia A. Rodionova , Nathan Tyburczy
IPC分类号： C40B30/04 , C40B60/12 , C40B30/00 , C40B50/00 , F04B43/00
CPC分类号： B01L3/502784 , B01L3/5023 , B01L3/502723 , B01L2200/0684 , B01L2200/10 , B01L2200/16 , B01L2300/0672 , B01L2300/0816 , B01L2300/0867 , B01L2400/0481 , B01L2400/0487 , B01L2400/0683
摘要： Flow-through assay reaction chamber (6) of cassette has back and forth liquid mixing in narrow gap (G) over array of capture agent (S), with net flow advance to waste confinement (19), produced by reversible pumps (3 or 12), operable with rolling diaphragm action with at least limited elastic recovery that advance sample or buffer liquids through conditioning paths (4A, 8, 8′, 9, 14, 15, 15′) before reaching the reaction chamber (6). A single pump produces accurate flow control, liquid conditioning, e.g., liquefying dry reagent from internal surfaces of flow-dividing material (14a, 15A, 15A′, e.g. open cell foam or frit), heating (4A), and air bubble removal (8, 8′, 9), as well as replenishment of reagent while accomplishing mixing within the flow-through reaction chamber (6). Lower viscosity buffer liquid is arranged to propel higher viscosity reagent, the flow-dividing storage material preserving reagent concentration. A blister pack (11) acts as a reversible pump (12) in producing accurate forward and backward flows with the net flow advance. Cascaded bubble traps (8, 9) on the cassette render the system tolerant of minor pumping error during cassette priming.
摘要翻译：盒的流通测定反应室（6）在捕获剂（S）的阵列上具有窄间隙（G）中的液体混合，净流量进入废水约束（19），由可逆泵（3或 12），其具有至少有限的弹性恢复的滚动隔膜作用，可在到达反应室（6）之前通过调节路径（4A，8,8'，9,14,15,15'）推进样品或缓冲液体。单个泵产生精确的流量控制，液体调节，例如从分流材料（14a，15A，15A'，例如开孔泡沫或玻璃料）的内表面液化干试剂，加热（4A）和气泡去除 8，8'，9），以及在流通反应室（6）内完成混合的同时补充试剂。较低粘度的缓冲液体配置为推进较高粘度的试剂，分流储存材料保留试剂浓度。泡罩包装（11）用作可逆泵（12），用于净流量提前产生精确的向前和向后的流动。卡带上的级联气泡捕集器（8,9）使得系统在盒式启动期间容忍较小的泵送误差。

9. 发明申请

US20060275852A1 Assays based on liquid flow over arrays 审中-公开
标题翻译：基于液体流动的分析
公开(公告)号：US20060275852A1
公开(公告)日：2006-12-07
申请号：US11262115
申请日：2005-10-27
申请人： Jean Montagu , Herman Deweerd , Roger Dowd , Natalia Rodionova , Peter Maimonis , Nathan Tyburczy
发明人： Jean Montagu , Herman Deweerd , Roger Dowd , Natalia Rodionova , Peter Maimonis , Nathan Tyburczy
IPC分类号： G01N33/53 , C12M1/34
CPC分类号： B01L3/502746 , B01L3/502715 , B01L3/502723 , B01L3/50273 , B01L3/502738 , B01L7/00 , B01L9/527 , B01L2200/027 , B01L2200/0684 , B01L2200/16 , B01L2300/0636 , B01L2300/0654 , B01L2300/0672 , B01L2300/0816 , B01L2300/0867 , B01L2300/0877 , B01L2300/1827 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655 , B01L2400/0683 , B01L2400/0688 , G01N33/54366 , Y10T436/117497
摘要： Cassette (50) performs assays, e.g. multiplexed protein biomarker assays. Wide, bubble-free, slow flows are produced from liquids stored on cassette (50), flowing over wide array (20) of ligand receptors on a capture surface. Flows of Reynolds Number less than about 1, preferably 1×10−1 to 5×10−3, are heated in region (34) preceding and including bubble removal system (128). Analyte is introduced through compressed septum (32). External actuations of displacement pumps (30, 37) and valves (137 A, B, and C) produce flows in response to flow-front optical sensors (150, 152). Elastic sheet provides pump and valve diaphragms and resilient expansion of mixing volume (131). Break-away cover portions are pistons. Heating is by conduction through cassette from external contact heater. Planar cassette body, when tilted from horizontal, enables upward flow from pumped storage (134, 135) to reaction (133) to waste (139), with buoyancy bubble removal before reaction. Reading of fluorescence is by external reader, employing calibration, control and reference features on capture surface. Extensive set of calibration features of differing intensities enables self-calibration.
摘要翻译：盒（50）进行测定。多重蛋白生物标志物测定。从储存在盒（50）上的液体，在捕获表面上的宽阵列（20）的配体受体上流动，产生宽而无气泡的慢流。小于约1，优选1×10 -1至5×10 -3以下的雷诺数流在气泡去除系统（128）之前和之后的区域（34）中被加热。通过压缩隔膜（32）引入分析物。位移泵（30,37）和阀（137A，B和C）的外部致动响应于前后光学传感器（150,152）产生流动。弹性片提供泵和阀膜片和弹性膨胀的混合量（131）。分离盖部分是活塞。加热是通过外部接触加热器的磁带传导。当从水平方向倾斜时，平面盒体能够在反应之前从泵送储存（134,135）到反应（133）到废物（139）的向上流动。通过外部读取器读取荧光，在捕获表面采用校准，控制和参考特征。广泛的不同强度的校准特征可实现自校准。

10. 发明授权

US08986983B2 Assays based on liquid flow over arrays 有权
标题翻译：基于液体流动的分析
公开(公告)号：US08986983B2
公开(公告)日：2015-03-24
申请号：US12876883
申请日：2010-09-07
申请人： Jean I. Montagu , Herman Deweerd , Roger Dowd , Natalia Rodionova , Peter Maimonis , Nathan Tyburczy
发明人： Jean I. Montagu , Herman Deweerd , Roger Dowd , Natalia Rodionova , Peter Maimonis , Nathan Tyburczy
IPC分类号： C12M1/34 , B01L3/00 , B01L7/00 , B01L9/00
CPC分类号： B01L3/502746 , B01L3/502715 , B01L3/502723 , B01L3/50273 , B01L3/502738 , B01L7/00 , B01L9/527 , B01L2200/027 , B01L2200/0684 , B01L2200/16 , B01L2300/0636 , B01L2300/0654 , B01L2300/0672 , B01L2300/0816 , B01L2300/0867 , B01L2300/0877 , B01L2300/1827 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655 , B01L2400/0683 , B01L2400/0688 , Y10T436/117497
摘要： Cassette (50) performs assays, e.g. multiplexed protein biomarker assays. Wide, bubble-free, slow flows are produced from liquids stored on cassette (50), flowing over wide array (20) of ligand receptors on a capture surface. Flows of Reynolds Number less than about 1, preferably 1×10−1 to 5×10−3, are heated in region (34) preceding and including bubble removal system (128). Analyte is introduced through compressed septum (32). External actuations of displacement pumps (30, 37) and valves (137 A, B, and C) produce flows in response to flow-front optical sensors (150, 152). Elastic sheet provides pump and valve diaphragms and resilient expansion of mixing volume (131). Break-away cover portions are pistons. Heating is by conduction through cassette from external contact heater. Planar cassette body, when tilted from horizontal, enables upward flow from pumped storage (134, 135) to reaction (133) to waste (139), with buoyancy bubble removal before reaction. Reading of fluorescence is by external reader, employing calibration, control and reference features on capture surface. Extensive set of calibration features of differing intensities enables self-calibration.
摘要翻译：盒（50）进行测定。多重蛋白生物标志物测定。从储存在盒（50）上的液体，在捕获表面上的宽阵列（20）的配体受体上流动，产生宽而无气泡的慢流。小于约1，优选1×10-1至5×10-3的雷诺数在包括气泡去除系统（128）之前的区域（34）中被加热。通过压缩隔膜（32）引入分析物。位移泵（30,37）和阀（137A，B和C）的外部致动响应于前后光学传感器（150,152）产生流动。弹性片提供泵和阀膜片和弹性膨胀的混合量（131）。分离盖部分是活塞。加热是通过外部接触加热器的磁带传导。当从水平方向倾斜时，平面盒体能够在反应之前从泵送储存（134,135）到反应（133）到废物（139）的向上流动。通过外部读取器读取荧光，在捕获表面采用校准，控制和参考特征。广泛的不同强度的校准特征可实现自校准。

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