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1. 发明申请

US20080026373A1 Assays Based On Light Emission From Analyte Complexes Within A Cassette 审中-公开
标题翻译：基于盒内分析物配合物的光发射测定
公开(公告)号：US20080026373A1
公开(公告)日：2008-01-31
申请号：US11460238
申请日：2006-07-26
申请人： Natalia A. Rodionova
发明人： Natalia A. Rodionova
IPC分类号： C12Q1/68 , C12M3/00
CPC分类号： G01N33/54366 , B01L3/5027 , B01L3/502715 , B01L3/502723 , B01L3/502738 , B01L2200/0678 , B01L2200/10 , B01L2300/0636 , B01L2300/0816 , B01L2400/0611 , B01L2400/0688 , G01N33/54373
摘要： Assays based on probes attached to surfaces enclosed within cassettes and cassettes and reading stations for the assays. After liquids flow over the probe or probe array to form an array of photo-emissive analyte complexes, and prior to reading, (a) liquid is removed by flow, and (b) a drying gas stream, is forced into the cassette and over the complexes for a drying interval to remove liquid residue. Heating assists the drying. Light from the analyte complexes is then read through a window of the cassette. The interval of drying may be of the order of about one minute. During a preceding wash phase, gas flow bursts through the gas inlet channel purge liquid contaminant. The probes e.g. may be oligonucleotides, peptides, polypeptides, proteins, antibodies, or small molecules (steroids, expression regulators, e.g. siRNA, or other ligands). A cassette has a passage leading from a bubble removal system to the probe-bearing surface and the desiccating gas stream is introduced to that passage. For wide arrays the common passage connects through a widening transition to a wide reaction chamber. A reader station includes an air pump and liquid pumping devices such as linear actuators to deflect liquid-pumping diaphragms of the cassette.
摘要翻译：基于附着在盒和盒中的表面的探针和用于测定的读取位点的测定。在液体流过探针或探针阵列以形成光发射分析物复合物的阵列之后，并且在读取之前，（a）通过流动除去液体，并且（b）干燥气流被迫进入盒中并过度该复合物用于干燥间隔以除去液体残留物。加热帮助干燥。然后从分析物复合物的光通过盒的窗口读取。干燥间隔可以是约1分钟。在前一洗涤阶段期间，气流通过气体入口通道吹扫液体污染物而爆裂。探针例如。可以是寡核苷酸，肽，多肽，蛋白质，抗体或小分子（类固醇，表达调节剂，例如siRNA或其他配体）。盒具有从气泡去除系统引导到探针承载表面的通道，并且干燥气流被引入该通道。对于宽阵列，公共通道通过扩大过渡连接到宽反应室。读取站包括空气泵和液体泵送装置，例如线性致动器以偏转盒的液体泵送膜片。

2. 发明申请

US20110207621A1 Assays Based on Liquid Flow over Arrays 审中-公开
标题翻译：基于液体流过阵列的测定
公开(公告)号：US20110207621A1
公开(公告)日：2011-08-25
申请号：US12918757
申请日：2009-02-20
申请人： Jean I. Montagu , Herman Deweerd , Natalia A. Rodionova , Nathan Tyburczy
发明人： Jean I. Montagu , Herman Deweerd , Natalia A. Rodionova , Nathan Tyburczy
IPC分类号： C40B30/04 , C40B60/12 , C40B30/00 , C40B50/00 , F04B43/00
CPC分类号： B01L3/502784 , B01L3/5023 , B01L3/502723 , B01L2200/0684 , B01L2200/10 , B01L2200/16 , B01L2300/0672 , B01L2300/0816 , B01L2300/0867 , B01L2400/0481 , B01L2400/0487 , B01L2400/0683
摘要： Flow-through assay reaction chamber (6) of cassette has back and forth liquid mixing in narrow gap (G) over array of capture agent (S), with net flow advance to waste confinement (19), produced by reversible pumps (3 or 12), operable with rolling diaphragm action with at least limited elastic recovery that advance sample or buffer liquids through conditioning paths (4A, 8, 8′, 9, 14, 15, 15′) before reaching the reaction chamber (6). A single pump produces accurate flow control, liquid conditioning, e.g., liquefying dry reagent from internal surfaces of flow-dividing material (14a, 15A, 15A′, e.g. open cell foam or frit), heating (4A), and air bubble removal (8, 8′, 9), as well as replenishment of reagent while accomplishing mixing within the flow-through reaction chamber (6). Lower viscosity buffer liquid is arranged to propel higher viscosity reagent, the flow-dividing storage material preserving reagent concentration. A blister pack (11) acts as a reversible pump (12) in producing accurate forward and backward flows with the net flow advance. Cascaded bubble traps (8, 9) on the cassette render the system tolerant of minor pumping error during cassette priming.
摘要翻译：盒的流通测定反应室（6）在捕获剂（S）的阵列上具有窄间隙（G）中的液体混合，净流量进入废水约束（19），由可逆泵（3或 12），其具有至少有限的弹性恢复的滚动隔膜作用，可在到达反应室（6）之前通过调节路径（4A，8,8'，9,14,15,15'）推进样品或缓冲液体。单个泵产生精确的流量控制，液体调节，例如从分流材料（14a，15A，15A'，例如开孔泡沫或玻璃料）的内表面液化干试剂，加热（4A）和气泡去除 8，8'，9），以及在流通反应室（6）内完成混合的同时补充试剂。较低粘度的缓冲液体配置为推进较高粘度的试剂，分流储存材料保留试剂浓度。泡罩包装（11）用作可逆泵（12），用于净流量提前产生精确的向前和向后的流动。卡带上的级联气泡捕集器（8,9）使得系统在盒式启动期间容忍较小的泵送误差。

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