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1. 发明申请

US20130055472A1 Methods for tissue culture and transformation of sugarcane 审中-公开
标题翻译：甘蔗组织培养和转化方法
公开(公告)号：US20130055472A1
公开(公告)日：2013-02-28
申请号：US13599003
申请日：2012-08-30
申请人： Myeong-Je Cho , Theodore M. Klein , Zuo-Yu Zhao
发明人： Myeong-Je Cho , Theodore M. Klein , Zuo-Yu Zhao
IPC分类号： A01H1/06 , C12N5/04
CPC分类号： C12N15/8205 , A01H4/00
摘要： Compositions and methods for the efficient transformation and regeneration of monocot plants are provided. The methods of transformation involve infection with Agrobacterium. In this manner, any gene of interest can be introduced into the monocot plant with high transformation efficiency and in low copy number. Transformed and regenerated monocot cells, tissues, plants, and seed are also provided. The invention encompasses regenerating transformed plants, transgenic seeds produced therefrom, and transgenic plants and transgenic seeds from subsequent generations.
摘要翻译：提供单叶植物有效转化和再生的组合物和方法。转化方法涉及土壤杆菌感染。以这种方式，任何感兴趣的基因可以以高转化效率和低拷贝数引入单子叶植物。还提供了转化和再生的单子叶植物细胞，组织，植物和种子。本发明包括再生转化的植物，由其产生的转基因种子，以及来自后代的转基因植物和转基因种子。

2. 发明授权

US07071382B2 Inhibitors of apoptosis proteins in plants 失效
标题翻译：植物中凋亡蛋白的抑制剂
公开(公告)号：US07071382B2
公开(公告)日：2006-07-04
申请号：US10679998
申请日：2003-10-07
申请人： Rebecca E. Cahoon , Perry G. Caimi , Theodore M. Klein , Michele Morgante , Hajime Sakai , Jennie Bih-Jien Shen , Scott V. Tingey
发明人： Rebecca E. Cahoon , Perry G. Caimi , Theodore M. Klein , Michele Morgante , Hajime Sakai , Jennie Bih-Jien Shen , Scott V. Tingey
IPC分类号： A01H1/00 , C07H21/04 , C07K14/415 , C12N5/14 , C12N9/00
CPC分类号： C12N15/8263 , C07K14/415
摘要： This invention relates to an isolated nucleic acid fragment encoding an apoptosis inhibitory protein. The invention also relates to the construction of a chimeric gene encoding all or a portion of the apoptosis inhibitory protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the apoptosis inhibitory protein in a transformed host cell.
摘要翻译：本发明涉及编码凋亡抑制蛋白的分离的核酸片段。本发明还涉及在正义或反义方向上编码编码全部或部分凋亡抑制蛋白的嵌合基因的构建，其中嵌合基因的表达导致在转化的宿主细胞中产生改变的凋亡抑制蛋白的水平。

3. 发明申请

US20120255070A1 AGROBACTERIUM-MEDIATED TRANSFORMATION OF DICOT PLANTS 有权
标题翻译： DICOT植物的农杆菌介导转化
公开(公告)号：US20120255070A1
公开(公告)日：2012-10-04
申请号：US13438077
申请日：2012-04-03
申请人： Hyeon-Je Cho , Linda A. Castle , Theodore M. Klein , Tai Minh , York Moy
发明人： Hyeon-Je Cho , Linda A. Castle , Theodore M. Klein , Tai Minh , York Moy
IPC分类号： A01H1/00 , C12N15/82 , C12N5/04
CPC分类号： C12N15/8205 , C12N1/20
摘要： Compositions and methods are provided for the efficient transformation of a dicot plant. More particularly, compositions and methods of the present invention find use in agriculture for Agrobacterium-mediated transformation of a dicotyledonous plant. The compositions include cultivation media comprising high concentrations of sucrose and glucose. The cultivation media find use in methods directed to Agrobacterium-mediated transformation of a dicot plant with a gene of interest. In this manner, any gene of interest can be introduced into a dicot plant with high transformation efficiency and reduced tissue necrosis.
摘要翻译：提供组合物和方法用于双子叶植物的有效转化。更具体地，本发明的组合物和方法用于农业用于双子叶植物的农杆菌介导的转化。组合物包括含有高浓度蔗糖和葡萄糖的培养基。栽培培养基可用于涉及农杆菌介导的具有感兴趣基因的双子叶植物转化的方法。以这种方式，可以将任何感兴趣的基因导入具有高转化效率和减少的组织坏死的双子叶植物。

4. 发明申请

US20110162111A1 METHOD FOR TRANSFORMING SOMATIC EMBRYOS 审中-公开
标题翻译：用于改变人造胚胎的方法
公开(公告)号：US20110162111A1
公开(公告)日：2011-06-30
申请号：US12976542
申请日：2010-12-22
申请人： Theodore M. Klein
发明人： Theodore M. Klein
IPC分类号： C12N15/87 , C12N15/82 , A01H5/00
CPC分类号： C12N15/8202 , C12N15/8206
摘要： Methods of transforming a plant cell with a nucleic acid of interest and regenerating plants therefrom are disclosed. The methods are particularly useful for the transformation of dicot or monocot mature somatic embryos.
摘要翻译：公开了用目的核酸转化植物细胞并从其再生植物的方法。该方法对双子叶植物或单子叶植物成熟体细胞胚的转化特别有用。

5. 发明申请

US20100083408A1 Gene Expression Modulating Element 有权
公开(公告)号：US20100083408A1
公开(公告)日：2010-04-01
申请号：US12626962
申请日：2009-11-30
申请人： Hochul Yi , Theodore M. Klein , Joanne E. Barton
发明人： Hochul Yi , Theodore M. Klein , Joanne E. Barton
IPC分类号： A01H5/00 , C07H21/04 , C12N5/10 , C07H21/00
CPC分类号： C12N15/8216
摘要： The present invention provides a method of screening for the purpose of obtaining gene expression modulating elements and gene insulator elements. The invention includes a method of identifying gene expression modulating elements and gene insulator elements through use of the following steps: a) locating intergenic regions of a plant genome that are flanked by a gene on each side that have differing gene expressions b) taking that intergenic region or a portion of that intergenic region and adding it to a cassette comprising an isolated gene c) introducing the cassette into a plant cell d) analyzing expression of the isolated gene. The present invention also includes identified sequences that act as gene expression modulating elements.

6. 发明申请

US20090235391A1 Alteration of Oil Traits in Plants 有权
标题翻译：植物油性状改变
公开(公告)号：US20090235391A1
公开(公告)日：2009-09-17
申请号：US12429498
申请日：2009-04-24
申请人： Stephen M. Allen , William B. Allen , Rebecca E. Cahoon , Sabine Epelbaum , Omolayo O. Famodu , Leslie T. Harvell , Todd J. Jones , Anthony J. Kinney , Theodore M. Klein , Changjiang Li , Igor Cunha Oliveira , Hajime Sakai , Bo Shen , Mitchell C. Tarczynski
发明人： Stephen M. Allen , William B. Allen , Rebecca E. Cahoon , Sabine Epelbaum , Omolayo O. Famodu , Leslie T. Harvell , Todd J. Jones , Anthony J. Kinney , Theodore M. Klein , Changjiang Li , Igor Cunha Oliveira , Hajime Sakai , Bo Shen , Mitchell C. Tarczynski
IPC分类号： C12N15/82 , C12N15/29 , A01H5/00 , A01H5/10 , A01H1/04
CPC分类号： C12N15/8247 , C07K14/415
摘要： The preparation and use of nucleic acid fragments useful in altering the oil phenotype in plants are disclosed. Chimeric constructs incorporating such nucleic acid fragments and suitable regulatory sequences can be used to create transgenic plants having altered lipid profiles. Methods for altering the oil phenotype in plants using such nucleic acid fragments also are disclosed.
摘要翻译：公开了可用于改变植物油表型的核酸片段的制备和使用。掺入此类核酸片段和合适调节序列的嵌合构建体可用于产生具有改变的脂质谱的转基因植物。还公开了使用这种核酸片段改变植物油表型的方法。

7. 发明授权

US07220894B2 “Methods of using leci transcriptional activator nucleic acids” 有权
标题翻译： “使用leci转录激活剂核酸的方法”
公开(公告)号：US07220894B2
公开(公告)日：2007-05-22
申请号：US10744149
申请日：2003-12-22
申请人： Keith S. Lowe , William J. Gordon-Kamm , Theodore M. Klein , Sonriza Rasco-Gaunt , Xifan Sun , George J. Hoerster
发明人： Keith S. Lowe , William J. Gordon-Kamm , Theodore M. Klein , Sonriza Rasco-Gaunt , Xifan Sun , George J. Hoerster
IPC分类号： C12N15/09 , C12N15/82 , A01H5/00
CPC分类号： C07K14/415 , C12N15/8209 , C12N15/8261 , C12N15/8287 , Y02A40/146
摘要： The invention provides methods of transforming plants and plant cells including recalcitrant cells with isolated nucleic acids encoding LEC1 transcriptional activators to induce transformation or regeneration efficiency, apomixis or somatic embryogenesis.
摘要翻译：本发明提供了用分离的编码LEC1转录激活因子的核酸转化植物和植物细胞（包括顽固细胞）以诱导转化或再生效率，无融合生殖或体细胞胚发生的方法。

8. 发明授权

US07888560B1 Plant transcription factors 有权
标题翻译：植物转录因子
公开(公告)号：US07888560B1
公开(公告)日：2011-02-15
申请号：US11899370
申请日：2007-09-04
申请人： Rebecca E. Cahoon , Theodore M. Klein
发明人： Rebecca E. Cahoon , Theodore M. Klein
IPC分类号： A01H1/00 , C07H21/04 , C12N15/29 , C07K14/415
CPC分类号： C12N15/8247 , C07K14/415 , C12N15/8261 , Y02A40/146
摘要： This invention relates to an isolated nucleic acid fragment encoding a transcription factor. The invention also relates to the construction of a chimeric gene encoding all or a portion of the transcription factor, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of accumulated oil in a transformed host cell.
摘要翻译：本发明涉及编码转录因子的分离的核酸片段。本发明还涉及在正义或反义方向上编码全部或部分转录因子的嵌合基因的构建，其中嵌合基因的表达导致转化的宿主细胞中积累的油的水平的改变。

9. 发明授权

US07655786B2 Gene expression modulating element 失效
标题翻译：基因表达调控元件
公开(公告)号：US07655786B2
公开(公告)日：2010-02-02
申请号：US11685347
申请日：2007-03-13
申请人： Hochul Yi , Theodore M. Klein , Joanne E. Barton
发明人： Hochul Yi , Theodore M. Klein , Joanne E. Barton
IPC分类号： C07H21/04 , C12N15/00
CPC分类号： C12N15/8216
摘要： The present invention provides a method of screening for the purpose of obtaining gene expression modulating elements and gene insulator elements. The invention includes a method of identifying gene expression modulating elements and gene insulator elements through use of the following steps: a) locating intergenic regions of a plant genome that are flanked by a gene on each side that have differing gene expressions b) taking that intergenic region or a portion of that intergenic region and adding it to a cassette comprising an isolated gene c) introducing the cassette into a plant cell d) analyzing expression of the isolated gene. The present invention also includes identified sequences that act as gene expression modulating elements.
摘要翻译：本发明提供了筛选用于获得基因表达调节元件和基因绝缘子元件的方法。本发明包括通过以下步骤鉴定基因表达调节元件和基因绝缘子元件的方法：a）定位两侧具有不同基因表达的每侧基因的植物基因组的基因间区域b）取该基因区域或该基因间区域的一部分，并将其加入到包含分离的基因的盒中c）将盒导入植物细胞中d）分析分离的基因的表达。本发明还包括用作基因表达调节元件的鉴定序列。

10. 发明授权

US07157621B2 Alteration of oil traits in plants 有权
标题翻译：改变植物油性状
公开(公告)号：US07157621B2
公开(公告)日：2007-01-02
申请号：US10183687
申请日：2002-06-27
申请人： Stephen M. Allen , William B. Allen , Rebecca E. Cahoon , Sabine Epelbaum , Omolayo O. Famodu , Leslie T. Harvell , Todd J. Jones , Anthony J. Kinney , Theodore M. Klein , Changjiang Li , Igor Cunha Oliveira , Hajime Sakai , Bo Shen , Mitchell C. Tarczynski
发明人： Stephen M. Allen , William B. Allen , Rebecca E. Cahoon , Sabine Epelbaum , Omolayo O. Famodu , Leslie T. Harvell , Todd J. Jones , Anthony J. Kinney , Theodore M. Klein , Changjiang Li , Igor Cunha Oliveira , Hajime Sakai , Bo Shen , Mitchell C. Tarczynski
IPC分类号： A01H5/00 , C12N15/82
CPC分类号： C12N15/8247 , C07K14/415
摘要： The preparation and use of nucleic acid fragments useful in altering the oil phenotype in plants are disclosed. Chimeric construct incorporating such nucleic acid fragments and suitable regulatory sequences can be used to create transgenic plants having altered lipid profiles. Methods for altering the oil phenotype in plants using such nucleic acid fragments also are disclosed.
摘要翻译：公开了可用于改变植物油表型的核酸片段的制备和使用。掺入此类核酸片段和合适调节序列的嵌合构建体可用于产生具有改变的脂质分布的转基因植物。还公开了使用这种核酸片段改变植物油表型的方法。

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