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    • 8. 发明授权
    • Gene expression modulating element
    • 基因表达调控元件
    • US07655786B2
    • 2010-02-02
    • US11685347
    • 2007-03-13
    • Hochul YiTheodore M. KleinJoanne E. Barton
    • Hochul YiTheodore M. KleinJoanne E. Barton
    • C07H21/04C12N15/00
    • C12N15/8216
    • The present invention provides a method of screening for the purpose of obtaining gene expression modulating elements and gene insulator elements. The invention includes a method of identifying gene expression modulating elements and gene insulator elements through use of the following steps: a) locating intergenic regions of a plant genome that are flanked by a gene on each side that have differing gene expressions b) taking that intergenic region or a portion of that intergenic region and adding it to a cassette comprising an isolated gene c) introducing the cassette into a plant cell d) analyzing expression of the isolated gene. The present invention also includes identified sequences that act as gene expression modulating elements.
    • 本发明提供了筛选用于获得基因表达调节元件和基因绝缘子元件的方法。 本发明包括通过以下步骤鉴定基因表达调节元件和基因绝缘子元件的方法:a)定位两侧具有不同基因表达的每侧基因的植物基因组的基因间区域b)取该基因 区域或该基因间区域的一部分,并将其加入到包含分离的基因的盒中c)将盒导入植物细胞中d)分析分离的基因的表达。 本发明还包括用作基因表达调节元件的鉴定序列。