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1. 发明授权

US07981677B2 Identifying a high oil phenotype in maize by detecting a marker locus genetically linked with a QTL6 region 有权
标题翻译：通过检测与QTL6区域遗传连锁的标记位点，识别玉米高油表型
公开(公告)号：US07981677B2
公开(公告)日：2011-07-19
申请号：US11680906
申请日：2007-03-01
申请人： William B. Allen , Bo Shen , Mitchell C. Tarczynski , Mark E. Williams , Peizhong Zheng , Gan-Yuan Zhong
发明人： William B. Allen , Bo Shen , Mitchell C. Tarczynski , Mark E. Williams , Peizhong Zheng , Gan-Yuan Zhong
IPC分类号： C12Q1/68 , C07H21/04 , G01N33/92
CPC分类号： A01H1/04 , C12N9/1029 , C12N15/8247 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/172 , Y10T436/104165
摘要： Compositions related to the quantitative trait locus 6 (QTL6) in maize and methods for their use are provided. The compositions are novel molecular marker loci that are genetically linked with QTL6 and which are associated with increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio of a plant or plant part thereof. These novel markers are characterized by the presence of at least one polymorphism relative to the corresponding marker locus from the QTL6 region of non-high-oil, non-high-oleic acid maize plants. In some embodiments, the novel marker loci comprise coding sequence for a maize DGAT1-2 polypeptide or biologically active variant thereof. The marker loci of the invention, and suitable fragments thereof, are useful in methods of the invention for manipulating oil and/or oleic acid content and/or oleic acid/lineolic acid ratio of a plant or plant part thereof, for marker-assisted selection of a plant, for example, a maize plant, or plant part thereof, having an increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio, and for marker-assisted breeding of the high oil and/or high oleic acid trait.
摘要翻译：提供了与玉米数量性状基因座6（QTL6）相关的成分及其使用方法。所述组合物是与QTL6遗传连锁的新型分子标记基因座，其与植物或植物部分的油含量增加和/或油酸含量增加和/或油酸/亚油酸含量增加相关。这些新型标记物的特征在于相对于非高油非高油酸玉米植物的QTL6区域的相应标记基因座存在至少一个多态性。在一些实施方案中，新的标记基因座包含玉米DGAT1-2多肽或其生物活性变体的编码序列。本发明的标记基因座及其合适的片段可用于本发明的操作用于标记辅助选择的植物或植物部分的油和/或油酸含量和/或油酸/线性酸比例的方法中具有增加的油含量和/或油酸含量增加和/或油酸/亚油酸比例增加的植物，例如玉米植物或其植物部分，以及高油的标记辅助育种和/或高油酸性状。

2. 发明授权

US07176009B2 Sucrose phosphate synthase 有权
标题翻译：蔗糖磷酸合成酶
公开(公告)号：US07176009B2
公开(公告)日：2007-02-13
申请号：US10641991
申请日：2003-08-14
申请人： Stephen M. Allen , Perry G. Caimi , Emil M. Orozco, Jr. , Mitchell C. Tarczynski
发明人： Stephen M. Allen , Perry G. Caimi , Emil M. Orozco, Jr. , Mitchell C. Tarczynski
IPC分类号： C12N9/10 , C12N1/20 , C12N15/00 , C12P21/04 , C12Q1/68 , C12Q1/48 , C07H21/04
CPC分类号： C12N9/1066 , C12N15/8245 , C12Y204/01014
摘要： This invention relates to an isolated nucleic acid fragment encoding a sucrose phosphate synthase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the sucrose phosphate synthase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the sucrose phosphate synthase in a transformed host cell.
摘要翻译：本发明涉及编码蔗糖磷酸合成酶的分离的核酸片段。本发明还涉及在正义或反义方向上构建编码全部或部分蔗糖磷酸合酶的嵌合基因，其中嵌合基因的表达导致在转化的宿主细胞中产生蔗糖磷酸合酶的改变水平。

3. 发明申请

US20110010795A9 AP2 DOMAIN TRANSCRIPTION FACTOR ODP2 (OVULE DEVELOPMENT PROTEIN 2) AND METHODS OF USE 有权
标题翻译： AP2域转录因子ODP2（OVULE DEVELOPMENT蛋白2）及其使用方法
公开(公告)号：US20110010795A9
公开(公告)日：2011-01-13
申请号：US12503482
申请日：2009-07-15
申请人： William J. Gordon-Kamm , Timothy G. Helentjaris , Keith S. Lowe , Bo Shen , Mitchell C. Tarczynski , Peizhong Zheng
发明人： William J. Gordon-Kamm , Timothy G. Helentjaris , Keith S. Lowe , Bo Shen , Mitchell C. Tarczynski , Peizhong Zheng
IPC分类号： C12N15/82 , C12N15/11 , C12N15/00 , A01H5/00 , C12N5/04
CPC分类号： C12N15/8243 , A01H1/08 , C07K14/415 , C12N15/8201 , C12N15/8205 , C12N15/8209 , C12N15/8223 , C12N15/8231 , C12N15/8233 , C12N15/8245 , C12N15/8247 , C12N15/8261 , C12N15/8287 , Y02A40/146
摘要： Methods and compositions for modulating plant development are provided. Nucleotide sequences and amino acid sequences encoding Ovule Development Protein 2 (ODP2) proteins are provided. The sequences can be used in a variety of methods including modulating development, developmental pathways, altering oil content in a plant, increasing transformation efficiencies, modulating stress tolerance, and modulating the regenerative capacity of a plant. Transformed plants, plant cells, tissues, and seed are also provided.
摘要翻译：提供了调节植物发育的方法和组合物。提供编码Ovule Development Protein 2（ODP2）蛋白的核苷酸序列和氨基酸序列。这些序列可以用于各种方法，包括调节发育，发育途径，改变植物中的油含量，提高转化效率，调节胁迫耐受性和调节植物的再生能力。还提供了转化的植物，植物细胞，组织和种子。

4. 发明授权

US07812216B2 Compositions related to the quantitative trait locus 6 (QTL6) in maize and methods of use 有权
标题翻译：与玉米的数量性状基因座6（QTL6）和使用方法有关的成分
公开(公告)号：US07812216B2
公开(公告)日：2010-10-12
申请号：US11680922
申请日：2007-03-01
申请人： William B. Allen , Bo Shen , Mitchell C. Tarczynski , Mark E. Williams , Peizhong Zheng , Gan-Yuan Zhong
发明人： William B. Allen , Bo Shen , Mitchell C. Tarczynski , Mark E. Williams , Peizhong Zheng , Gan-Yuan Zhong
IPC分类号： A01H1/00 , A01H5/00 , A01H5/10 , C12N15/82
CPC分类号： C12N15/8247 , C12N9/1029 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/172
摘要： Compositions related to the quantitative trait locus 6 (QTL6) in maize and methods for their use are provided. The compositions are novel molecular marker loci that are genetically linked with QTL6 and which are associated with increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio of a plant or plant part thereof. These novel markers are characterized by the presence of at least one polymorphism relative to the corresponding marker locus from the QTL6 region of non-high-oil, non-high-oleic acid maize plants. In some embodiments, the novel marker loci comprise coding sequence for a maize DGAT1-2 polypeptide or biologically active variant thereof. The marker loci of the invention, and suitable fragments thereof, are useful in methods of the invention for manipulating oil and/or oleic acid content and/or oleic acid/lineolic acid ratio of a plant or plant part thereof, for marker-assisted selection of a plant, for example, a maize plant, or plant part thereof, having an increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio, and for marker-assisted breeding of the high oil and/or high oleic acid trait.
摘要翻译：提供了与玉米数量性状基因座6（QTL6）相关的成分及其使用方法。所述组合物是与QTL6遗传连锁的新型分子标记基因座，其与植物或植物部分的油含量增加和/或油酸含量增加和/或油酸/亚油酸含量增加相关。这些新型标记物的特征在于相对于非高油非高油酸玉米植物的QTL6区域的相应标记基因座存在至少一个多态性。在一些实施方案中，新的标记基因座包含玉米DGAT1-2多肽或其生物活性变体的编码序列。本发明的标记基因座及其合适的片段可用于本发明的操作用于标记辅助选择的植物或植物部分的油和/或油酸含量和/或油酸/线性酸比例的方法中具有增加的油含量和/或增加的油酸含量和/或油酸/亚油酸比例增加的植物，例如玉米植物或其植物部分，以及用于高油的标记辅助育种和/或高油酸性状。

5. 发明申请

US20090235391A1 Alteration of Oil Traits in Plants 有权
标题翻译：植物油性状改变
公开(公告)号：US20090235391A1
公开(公告)日：2009-09-17
申请号：US12429498
申请日：2009-04-24
申请人： Stephen M. Allen , William B. Allen , Rebecca E. Cahoon , Sabine Epelbaum , Omolayo O. Famodu , Leslie T. Harvell , Todd J. Jones , Anthony J. Kinney , Theodore M. Klein , Changjiang Li , Igor Cunha Oliveira , Hajime Sakai , Bo Shen , Mitchell C. Tarczynski
发明人： Stephen M. Allen , William B. Allen , Rebecca E. Cahoon , Sabine Epelbaum , Omolayo O. Famodu , Leslie T. Harvell , Todd J. Jones , Anthony J. Kinney , Theodore M. Klein , Changjiang Li , Igor Cunha Oliveira , Hajime Sakai , Bo Shen , Mitchell C. Tarczynski
IPC分类号： C12N15/82 , C12N15/29 , A01H5/00 , A01H5/10 , A01H1/04
CPC分类号： C12N15/8247 , C07K14/415
摘要： The preparation and use of nucleic acid fragments useful in altering the oil phenotype in plants are disclosed. Chimeric constructs incorporating such nucleic acid fragments and suitable regulatory sequences can be used to create transgenic plants having altered lipid profiles. Methods for altering the oil phenotype in plants using such nucleic acid fragments also are disclosed.
摘要翻译：公开了可用于改变植物油表型的核酸片段的制备和使用。掺入此类核酸片段和合适调节序列的嵌合构建体可用于产生具有改变的脂质谱的转基因植物。还公开了使用这种核酸片段改变植物油表型的方法。

6. 发明授权

US07579529B2 AP2 domain transcription factor ODP2 (ovule development protein 2) and methods of use 有权
标题翻译： AP2结构域转录因子ODP2（胚珠发育蛋白2）及其使用方法
公开(公告)号：US07579529B2
公开(公告)日：2009-08-25
申请号：US11045802
申请日：2005-01-28
申请人： William J. Gordon-Kamm , Timothy G. Helentjaris , Keith S. Lowe , Bo Shen , Mitchell C. Tarczynski , Peizhong Zheng
发明人： William J. Gordon-Kamm , Timothy G. Helentjaris , Keith S. Lowe , Bo Shen , Mitchell C. Tarczynski , Peizhong Zheng
IPC分类号： C12N15/29 , C12N15/82 , A01H5/00
CPC分类号： C12N15/8243 , A01H1/08 , C07K14/415 , C12N15/8201 , C12N15/8205 , C12N15/8209 , C12N15/8223 , C12N15/8231 , C12N15/8233 , C12N15/8245 , C12N15/8247 , C12N15/8261 , C12N15/8287 , Y02A40/146
摘要： Methods and compositions for modulating plant development are provided. Nucleotide sequences and amino acid sequences encoding Ovule Development Protein 2 (ODP2) proteins are provided. The sequences can be used in a variety of methods including modulating development, developmental pathways, altering oil content in a plant, increasing transformation efficiencies, modulating stress tolerance, and modulating the regenerative capacity of a plant. Transformed plants, plant cells, tissues, and seed are also provided.
摘要翻译：提供了调节植物发育的方法和组合物。提供编码Ovule Development Protein 2（ODP2）蛋白的核苷酸序列和氨基酸序列。这些序列可以用于各种方法，包括调节发育，发育途径，改变植物中的油含量，提高转化效率，调节胁迫耐受性和调节植物的再生能力。还提供了转化的植物，植物细胞，组织和种子。

7. 发明授权

US07560623B2 Enzymatic methods for modulating the levels of organic sulfur compounds in plants 有权
标题翻译：用于调节植物中有机硫化合物水平的酶法
公开(公告)号：US07560623B2
公开(公告)日：2009-07-14
申请号：US11365135
申请日：2006-03-01
申请人： Mitchell C. Tarczynski , Changjiang Li , Bo Shen
发明人： Mitchell C. Tarczynski , Changjiang Li , Bo Shen
IPC分类号： C12N15/82 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8243 , C12N15/8242
摘要： Methods for modulating levels of at least one organic sulfur compound in plants are provided. Also provided are plants and seeds produced by the methods. The methods comprise stably transforming a plant with a DNA construct encoding a cystathionine gamma synthase enzyme, a serine acetyl transferase enzyme and/or other sulfur assimilating enzymes capable of altering the level of at least one organic sulfur compound.
摘要翻译：提供了调节植物中至少一种有机硫化合物水平的方法。还提供了通过这些方法生产的植物和种子。所述方法包括用编码胱硫醚γ-合成酶的DNA构建体，丝氨酸乙酰转移酶和/或能够改变至少一种有机硫化合物的水平的其它硫同化酶稳定转化植物。

8. 发明授权

US07038109B1 Enzymatic methods for modulating the levels of organic sulfur compounds in plants 有权
公开(公告)号：US07038109B1
公开(公告)日：2006-05-02
申请号：US10108739
申请日：2002-03-29
申请人： Mitchell C. Tarczynski , Changjiang Li , Bo Shen
发明人： Mitchell C. Tarczynski , Changjiang Li , Bo Shen
IPC分类号： C12N15/82 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8243 , C12N15/8242
摘要： Methods for modulating levels of at least one organic sulfur compound in plants are provided. Also provided are plants and seeds produced by the methods. The methods comprise stably transforming a plant with a DNA construct encoding a cystathionine gamma synthase enzyme, a serine acetyl transferase enzyme and/or other sulfur assimilating enzymes capable of altering the level of at least one organic sulfur compound.

9. 发明授权

US6080913A Binary methods of increasing accumulation of essential amino acids in seeds 失效
标题翻译：增加种子中必需氨基酸积累的二元方法
公开(公告)号：US6080913A
公开(公告)日：2000-06-27
申请号：US719500
申请日：1996-09-25
申请人： Mitchell C. Tarczynski , Paul E. Staswick
发明人： Mitchell C. Tarczynski , Paul E. Staswick
IPC分类号： C07K14/415 , C12N15/82 , A01H5/00 , A01H5/10 , C12N5/04 , C12N15/29
CPC分类号： C07K14/415 , C12N15/8251 , C12N15/8253 , C12N15/8254
摘要： The present invention provides methods for genetically modifying plants to increase the levels of essential amino acids in seed. The present methods involve a combination of:a) providing an increased reservoir or source of a target amino acid population in vegetative tissue; withb) a complementary protein sink.
摘要翻译：本发明提供用于遗传修饰植物以增加种子中必需氨基酸水平的方法。本方法涉及以下组合：a）在营养组织中提供增加的储存物或来源的目标氨基酸群; 与b）互补的蛋白质水槽。

10. 发明授权

US08785726B2 Alteration of oil traits in plants 有权
标题翻译：改变植物油性状
公开(公告)号：US08785726B2
公开(公告)日：2014-07-22
申请号：US12429498
申请日：2009-04-24
申请人： William B Allen , Karlene H Butler , Rebecca E Cahoon , Sabine U Epelbaum , Changjiang Li , Igor Cunha De Oliveira , Hajime Sakai , Bo Shen , Mitchell C Tarczynski
发明人： William B Allen , Karlene H Butler , Rebecca E Cahoon , Sabine U Epelbaum , Changjiang Li , Igor Cunha De Oliveira , Hajime Sakai , Bo Shen , Mitchell C Tarczynski
IPC分类号： C12N15/82 , A01H5/00 , C07H21/04
CPC分类号： C12N15/8247 , C07K14/415
摘要： The preparation and use of nucleic acid fragments useful in altering the oil phenotype in plants are disclosed. Chimeric constructs incorporating such nucleic acid fragments and suitable regulatory sequences can be used to create transgenic plants having altered lipid profiles. Methods for altering the oil phenotype in plants using such nucleic acid fragments also are disclosed.
摘要翻译：公开了可用于改变植物油表型的核酸片段的制备和使用。掺入此类核酸片段和合适调节序列的嵌合构建体可用于产生具有改变的脂质谱的转基因植物。还公开了使用这种核酸片段改变植物油表型的方法。

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