专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US09481890B2 Modification of plant development and morphology 有权
标题翻译：植物发育和形态的修改
公开(公告)号：US09481890B2
公开(公告)日：2016-11-01
申请号：US14041679
申请日：2013-09-30
申请人： British American Tobacco (Investments) Limited
发明人： Christopher John Robert Thomas , Martin Richard Ward
IPC分类号： C12N15/87 , C12N15/82
CPC分类号： C12N15/8261 , C12N15/8226 , C12N15/8229 , C12N15/8237 , C12N15/8263 , Y02A40/146
摘要： A method of modifying morphology in a plant comprising introducing into a plant at least one chimaeric gene comprising a promoter sequence operably associated with a nucleic acid sequence, the promoter sequence being operable to direct expression in specific cells of the plant and the nucleic acid sequence encoding at least one gene product capable of altering the metabolism of or causing death of the specific cells and/or nearby cells. In particular, the promoter sequence is operable to direct expression in lateral bud or lateral shoot and the nucleic acid encoding at least one gene product capable of disrupting the metabolism of or causing the death of the lateral bud or lateral shoot or nearby cells. Preferably the promoter sequence comprises the sequence shown as SEQ ID No. 1 or SEQ ID No. 7 or SEQ ID No. 4, or a part thereof capable of regulating expression of a gene, or a sequence having at least 60%, preferably at least 75%, homology to SEQ ID No. 1 or SEQ ID No. 7 and being capable of regulating expression of a gene.
摘要翻译：一种在植物中修饰形态的方法，包括向植物中引入至少一个嵌合基因，其包含可操作地与核酸序列相关的启动子序列，所述启动子序列可操作以指导在植物的特定细胞中的表达和编码的核酸序列至少一种能够改变特定细胞和/或附近细胞的代谢或引起死亡的基因产物。特别地，启动子序列可操作以指导侧芽或侧枝中的表达，以及编码能够破坏侧芽或侧芽或附近细胞的代谢或引起侧芽或侧枝或附近细胞死亡的至少一种基因产物的核酸。优选地，启动子序列包含SEQ ID No.1或SEQ ID No.7或SEQ ID No.4所示的序列，或其能调节基因表达的部分或具有至少60％的序列，优选在至少75％与SEQ ID No.1或SEQ ID No.7具有同源性，并且能够调节基因的表达。

2. 发明授权

US09382548B2 Nanocarrier based plant transfection and transduction 有权
标题翻译：基于纳米载体的植物转染和转导
公开(公告)号：US09382548B2
公开(公告)日：2016-07-05
申请号：US14135776
申请日：2013-12-20
申请人： AGRICULTURE AND AGRI-FOOD CANADA
发明人： Francois Eudes , Archana Chugh
IPC分类号： C12N15/82 , C12N15/87
CPC分类号： C12N15/8202 , C07K2319/10 , C12N15/8206 , C12N15/8218 , C12N15/8263 , C12N15/87
摘要： The present invention provides a novel method for the transduction and/or transfection of plant cells. Cell-penetrating peptides (CPPs) have been successfully employed as nanocarriers to deliver proteins and oligonucleotides to single plant cell microspores as well as multi-cellular zygotic embryos. The efficiency of CPP internalization and further delivery of a macromolecular cargo comprising a protein and/or an oligonucleotide can be enhanced by permeabilization of the zygotic embryos.
摘要翻译：本发明提供了用于转导和/或转染植物细胞的新方法。细胞穿透肽（CPP）已经成功地用作纳米载体，以将蛋白质和寡核苷酸递送到单个植物细胞小孢子以及多细胞合子胚胎。包含蛋白质和/或寡核苷酸的大分子货物的CPP内化和进一步递送的效率可以通过合子胚的透化来增强。

3. 发明授权

US09297019B2 Chimeric promoters capable of mediating gene expression in plants upon pathogen infection and uses thereof 有权
公开(公告)号：US09297019B2
公开(公告)日：2016-03-29
申请号：US13930345
申请日：2013-06-28
申请人： MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V.
发明人： Christoph Kirsch , Elke Logemann , Klaus Hahlbrock , Paul Rushton , Imre Somssich
IPC分类号： C12N15/82 , C12N15/10 , C12N15/63 , C07K14/415 , C07K16/16 , C12Q1/02
CPC分类号： C12N15/8216 , C07K14/415 , C07K16/16 , C12N15/8239 , C12N15/8263 , C12N15/8279 , C12N15/8283 , C12Q1/025
摘要： Described are synthetic promoters capable of mediating gene expression in plants upon pathogen infection. Furthermore, recombinant genes and vectors comprising said chimeric promoters as well as host cells transformed with such chimeric promoters, recombinant genes, or vectors are provided. Additionally, diagnostic compositions and kits comprising such chimeric promoters, recombinant genes, vectors or cells are described. Provided are further methods for the identification of compounds being capable of activating or inhibiting genes that are specifically expressed in plants upon pathogen infection employing the above described means. Furthermore, transgenic plant cells, plant tissue, and plants containing the above-described chimeric promoters, recombinant genes, and vectors as well as the use of the aforementioned chimeric promoters, recombinant genes, vectors and/or compounds identified by the method of the invention in plant cell and tissue culture, plant breeding, and/or agriculture are described.

4. 发明申请

US20150159170A1 NOVEL PLANT-DERIVED CIS-REGULATORY ELEMENTS FOR THE DEVELOPMENT OF PATHOGEN-RESPONSIVE CHIMERIC PROMOTORS 审中-公开
标题翻译：新型植物衍生的独联体监管元素，用于发展病原性重要的发起者
公开(公告)号：US20150159170A1
公开(公告)日：2015-06-11
申请号：US14380545
申请日：2013-02-26
申请人： KWS SAAT AG
发明人： Dietmar Stahl , Stephanie Meinecke
IPC分类号： C12N15/82
CPC分类号： C12N15/8279 , C12N15/8239 , C12N15/8263 , C12N15/8282
摘要： The present invention provides pathogen-resistant transgenic plants which exhibit a resistance protein-mediated pathogen defence reaction in a cell of the plant under stringent control owing to a pathogen infection. In this case, as inducer of the pathogen defence, parts of avirulence proteins are used, the stable integration of which is possible by means of usual transformation methods. In addition, the invention relates to a composition of nucleic acids which, after integration into the genome of a plant, mediates the pathogen resistance therein, to a method for producing a pathogen-resistant plant, and to plants for producing a pathogen-resistant plant.
摘要翻译：本发明提供了病原体抗性的转基因植物，其由于病原体感染在严格控制下在植物细胞中表现出抗性蛋白介导的病原体防御反应。在这种情况下，作为病原体防御的诱导剂，使用部分无毒素蛋白质，其通过常规转化方法可以稳定地整合其中。此外，本发明涉及核酸组合物，其在整合到植物的基因组中后，介导其中的病原体抗性，用于产生抗病原体植物的方法，以及用于产生病原体抗性植物的植物。

5. 发明申请

US20150152430A1 COMPOSITIONS AND METHODS FOR THE EXPRESSION OF A SEQUENCE IN A REPRODUCTIVE TISSUE OF A PLANT 审中-公开
标题翻译：在植物生殖组织中表达序列的组合物和方法
公开(公告)号：US20150152430A1
公开(公告)日：2015-06-04
申请号：US14612420
申请日：2015-02-03
申请人： Pioneer Hi-Bred International, Inc.
发明人： Marc C. Albertsen , Mark Alan Chamberlin , Tim Wayne Fox , Shai Joshua Lawit , Brian Roy Loveland
IPC分类号： C12N15/82 , C07K14/415
CPC分类号： C12N15/8233 , C07K14/415 , C12N15/8234 , C12N15/8263 , C12N15/829
摘要： Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include promoter sequences with direct expression in an egg cell or embryonic cell-preferred manner. Such compositions find use in, for example, a method for expressing a heterologous nucleotide sequence in a plant; detection of specific cell types in the ovule and targeted ablation of specific cell types.
摘要翻译：提供了用于调节植物中异源核苷酸序列表达的组合物和方法。组合物包括在卵细胞中直接表达的启动子序列或胚胎细胞优选的方式。这样的组合物可用于例如在植物中表达异源核苷酸序列的方法; 检测胚珠中的特定细胞类型和特定细胞类型的靶向消融。

6. 发明授权

US08829170B2 Construct capable of release in closed circular form from a larger nucleotide sequence permitting site specific expression and/or developmentally regulated expression of selected genetic sequences 有权
标题翻译：能够以允许位点特异性表达和/或发育调节所选遗传序列表达的较大核苷酸序列以闭环形式释放的构建体
公开(公告)号：US08829170B2
公开(公告)日：2014-09-09
申请号：US12948005
申请日：2010-11-17
申请人： James Langham Dale , Benjamin Dugdale , Greg John Hafner , Scott Richard Hermann , Douglas Kenneth Becker , Robert Maxwell Harding , Srimek Chowpongpang
发明人： James Langham Dale , Benjamin Dugdale , Greg John Hafner , Scott Richard Hermann , Douglas Kenneth Becker , Robert Maxwell Harding , Srimek Chowpongpang
IPC分类号： C07H21/04
CPC分类号： C12N15/8282 , C12N15/79 , C12N15/8203 , C12N15/8216 , C12N15/8257 , C12N15/8263 , C12N15/8283
摘要： The present invention relates generally to constructs and in particular genetic constructs comprising polynucleotide sequences capable of release in covalently closed, circular form from a larger nucleotide sequence such as, but not limited to, a genome of a eukaryotic cell. Preferably, once released, a polynucleotide sequence is reconstituted in a form which permits expression of the polynucleotide sequence. In one embodiment, the reconstituted polynucleotide sequence comprises a coding sequence with all or part of an extraneous nucleotide such as, but not limited to, an intronic sequence or other splice signal inserted therein. Expression and in particular transcription of the coding sequence involves splicing out the extraneous sequence. The release and circularization is generally in response to a stimulus such as a protein-mediated stimulus. More particularly, the protein is a viral or prokaryotic or eukaryotic derived protein or developmentally and/or tissue specific regulated protein.
摘要翻译：本发明一般涉及构建体，特别是包含能够以较大核苷酸序列（例如但不限于真核细胞的基因组）以共价闭合的环形形式释放的多核苷酸序列的遗传构建体。优选地，一旦释放，以允许多核苷酸序列表达的形式重构多核苷酸序列。在一个实施方案中，重构的多核苷酸序列包含具有全部或部分外来核苷酸的编码序列，例如但不限于插入其中的内含子序列或其他剪接信号。表达，特别是编码序列的转录涉及剪接外来序列。释放和环化通常响应于诸如蛋白质介导的刺激的刺激。更具体地，蛋白质是病毒或原核或真核衍生的蛋白质或发育和/或组织特异性调节蛋白。

7. 发明授权

US08685736B2 Poly ADP-ribose polymerase sequences and their uses 有权
标题翻译：聚ADP-核糖聚合酶序列及其用途
公开(公告)号：US08685736B2
公开(公告)日：2014-04-01
申请号：US13244200
申请日：2011-09-23
申请人： Elena Babiychuk , Kushnir Sergei , Marc De Block
发明人： Elena Babiychuk , Kushnir Sergei , Marc De Block
IPC分类号： C12N15/82 , C12P19/34 , C12Q1/68
CPC分类号： C12N15/8289 , C12N9/1077 , C12N15/8263 , Y10T436/143333
摘要： The invention provides for the use of isolated polynucleotides encoding maize poly (ADP-ribose) polymerase (PARP) proteins to identify endogenous PARP encoding genes or cDNAs from a plant and to produce plant cells and plants with high vigor.
摘要翻译：本发明提供了使用编码玉米聚（ADP-核糖）聚合酶（PARP）蛋白的分离的多核苷酸来鉴定来自植物的内源性PARP编码基因或cDNA并产生具有高活力的植物细胞和植物。

8. 发明授权

US08580943B2 Chimeric promoters capable of mediating gene expression in plants upon pathogen infection and uses thereof 有权
标题翻译：能够在病原体感染中介导植物基因表达的嵌合启动子及其用途
公开(公告)号：US08580943B2
公开(公告)日：2013-11-12
申请号：US13225509
申请日：2011-09-05
申请人： Christoph Kirsch , Elke Logemann , Klaus Hahlbrock , Paul Rushton , Imre Somssich
发明人： Christoph Kirsch , Elke Logemann , Klaus Hahlbrock , Paul Rushton , Imre Somssich
IPC分类号： C12N15/82 , C12N15/10 , C12N15/63
CPC分类号： C12N15/8216 , C07K14/415 , C07K16/16 , C12N15/8239 , C12N15/8263 , C12N15/8279 , C12N15/8283 , C12Q1/025
摘要： Described are synthetic promoters capable of mediating gene expression in plants upon pathogen infection. Furthermore, recombinant genes and vectors comprising said chimeric promoters as well as host cells transformed with such chimeric promoters, recombinant genes, or vectors are provided. Additionally, diagnostic compositions and kits comprising such chimeric promoters, recombinant genes, vectors or cells are described. Provided are further methods for the identification of compounds being capable of activating or inhibiting genes that are specifically expressed in plants upon pathogen infection employing the above described means. Furthermore, transgenic plant cells, plant tissue, and plants containing the above-described chimeric promoters, recombinant genes, and vectors as well as the use of the aforementioned chimeric promoters, recombinant genes, vectors and/or compounds identified by the method of the invention in plant cell and tissue culture, plant breeding, and/or agriculture are described.
摘要翻译：描述了能够在病原体感染后介导植物中的基因表达的合成启动子。此外，提供了包含所述嵌合启动子的重组基因和载体以及用这种嵌合启动子，重组基因或载体转化的宿主细胞。另外，描述了包含这种嵌合启动子，重组基因，载体或细胞的诊断组合物和试剂盒。提供了用于鉴定能够激活或抑制在使用上述方法的病原体感染后在植物中特异性表达的基因的化合物的其它方法。此外，转基因植物细胞，植物组织和含有上述嵌合启动子，重组基因和载体的植物以及通过本发明的方法鉴定的上述嵌合启动子，重组基因，载体和/或化合物的用途描述了植物细胞和组织培养，植物育种和/或农业。

9. 发明申请

US20130180006A1 Pollen Preferred Promoters and Methods of Use 有权
标题翻译：花粉优选的启动子和使用方法
公开(公告)号：US20130180006A1
公开(公告)日：2013-07-11
申请号：US13445288
申请日：2012-04-12
申请人： Andrew Mark Cigan , Shai J. Lawit
发明人： Andrew Mark Cigan , Shai J. Lawit
IPC分类号： C12N15/82 , A01H5/10 , C12N5/10
CPC分类号： C12N15/8231 , C12N9/1077 , C12N9/22 , C12N15/8261 , C12N15/8263 , C12Y204/0203 , C12Y301/27
摘要： Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include nucleotide sequences encompasses a strong pollen preferred promoter which drives strong, specific expression of gene products in pollen. Also provided is a method for expressing a heterologous nucleotide sequence in a plant using a promoter sequence disclosed herein.
摘要翻译：提供了用于调节植物中异源核苷酸序列表达的组合物和方法。组合物包括核苷酸序列包括强花粉优选启动子，其驱动花粉中基因产物的强烈，特异性表达。还提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。

10. 发明申请

US20120311736A1 SELECTIVE ABLATION OF DIPLOID EMBRYOS 审中-公开
标题翻译：胆固醇的选择性消除
公开(公告)号：US20120311736A1
公开(公告)日：2012-12-06
申请号：US13586893
申请日：2012-08-16
申请人： Mark E. Williams , William J. Gordon-Kamm
发明人： Mark E. Williams , William J. Gordon-Kamm
IPC分类号： A01H1/04 , A01H1/02 , A01H5/00
CPC分类号： C12N15/8263 , A01H1/08 , C12N15/8217 , C12N15/8287
摘要： Methods of selecting haploid embryos are disclosed. Methods of producing haploid embryos and non-viable diploid embryos on a plant are provided. Methods for selecting haploid embryos produced from haploid inducer maize lines are provided. Methods for producing improved maize haploid inducer lines are disclosed. Maize haploid inducer lines comprising transgenes causing ablated or abnormal diploid embryos are disclosed.
摘要翻译：公开了选择单倍体胚胎的方法。提供了在植物上产生单倍体胚胎和不可行的二倍体胚胎的方法。提供了用于选择由单倍体诱导物玉米品系生产的单倍体胚胎的方法。公开了生产改良的玉米单倍体诱导物系的方法。公开了包含导致烧蚀或异常二倍体胚的转基因的玉米单倍体诱导物系。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式