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1. 发明申请

US20080242554A1 NUCLEOTIDE PRIMER SET AND NUCLEOTIDE PROBE FOR DETECTING GENOTYPE OF METHYLENE TETRAHYDROFOLATE REDUCTASE (MTHFR) 失效
标题翻译：用于检测甲基四氢叶酸还原酶（MTHFR）基因组的核酸酶和核苷酸探针
公开(公告)号：US20080242554A1
公开(公告)日：2008-10-02
申请号：US12015645
申请日：2008-01-17
申请人： Naoko NAKAMURA , Keiko Ito , Masayoshi Takahashi , Koji Hashimoto , Nobuhiro Gemma
发明人： Naoko NAKAMURA , Keiko Ito , Masayoshi Takahashi , Koji Hashimoto , Nobuhiro Gemma
IPC分类号： C12Q1/68 , C40B30/04 , C07H21/00 , C40B40/06
CPC分类号： C12Q1/6827 , C12Q2537/137 , C12Q2525/301
摘要： There is provided is a nucleotide primer set for LAMP amplification used for detecting genotypes of single-nucleotide polymorphisms C677T and A1298C of an MTHFR gene. There is also provided a nucleotide probe for detecting an amplification product amplified by the primer set according to the present invention. There is also provided a method of detecting the genotypes of the single-nucleotide polymorphisms C677T and A1298C in the MTHFR gene, by using the primer set according to the present invention.
摘要翻译：提供了用于检测MTHFR基因的单核苷酸多态性C677T和A1298C的基因型的LAMP扩增的核苷酸引物组。还提供了用于检测根据本发明的引物组扩增的扩增产物的核苷酸探针。还提供了通过使用根据本发明的引物组来检测MTHFR基因中单核苷酸多态性C677T和A1298C的基因型的方法。

2. 发明申请

US20100248210A1 Nucleic acid primer set for detection of drug-resistant strain of hepatitis B virus, assay kit, and method of detecting drug-resistant strain of hepatitis B virus 审中-公开
标题翻译：用于检测乙型肝炎病毒耐药菌株的核酸引物组，测定试剂盒和检测乙型肝炎病毒耐药菌株的方法
公开(公告)号：US20100248210A1
公开(公告)日：2010-09-30
申请号：US12382733
申请日：2009-03-23
申请人： Masayoshi Takahashi , Michie Hashimoto , Keiko Ito , Keiko Kizu , Shunji Mishiro , Kazuaki Takahashi , Kazunori Miyazaki , Koji Hashimoto , Nobuhiro Gemma
发明人： Masayoshi Takahashi , Michie Hashimoto , Keiko Ito , Keiko Kizu , Shunji Mishiro , Kazuaki Takahashi , Kazunori Miyazaki , Koji Hashimoto , Nobuhiro Gemma
IPC分类号： C12Q1/70
CPC分类号： C12Q1/706
摘要： The invention provides a method of detecting a drug-resistant strain of hepatitis B virus, including amplifying a hepatitis B virus nucleic acid in a sample solution by LAMP with a primer set to yield an amplification product, and hybridizing the amplification product with a probe containing a polynucleotide derived from a drug-resistant strain and/or a probe containing a polynucleotide derived from a drug-nonresistant strain, to detect a drug-resistant strain of hepatitis B virus.
摘要翻译：本发明提供一种检测乙型肝炎病毒的耐药性菌株的方法，包括通过具有引物组的LAMP扩增样品溶液中的乙型肝炎病毒核酸以产生扩增产物，并将扩增产物与含有衍生自耐药菌株的多核苷酸和/或含有衍生自药物不耐药菌株的多核苷酸的探针，以检测乙型肝炎病毒的耐药菌株。

3. 发明申请

US20070037199A1 Individual discriminating method, as well as array, apparatus and system for individual discriminating test 审中-公开
标题翻译：个人鉴别方法，以及个人鉴别试验的阵列，仪器和系统
公开(公告)号：US20070037199A1
公开(公告)日：2007-02-15
申请号：US11514894
申请日：2006-09-05
申请人： Masayoshi Takahashi , Koji Hashimoto
发明人： Masayoshi Takahashi , Koji Hashimoto
IPC分类号： C12Q1/68 , G06F19/00 , C12M3/00
CPC分类号： C12Q1/6827 , C12Q2565/607 , C12Q2565/518 , C12Q2535/131
摘要： The invention provides a method of individual discriminating. The method includes selecting and using single nucleotide polymorphism satisfying any one of the conditions defined by this invention.
摘要翻译：本发明提供了个体鉴别的方法。该方法包括选择和使用满足本发明定义的任一条件的单核苷酸多态性。

4. 发明授权

US07728119B2 Nucleotide primer set and nucleotide probe for detecting genotype of methylene tetrahydrofolate reductase (MTHFR) 失效
标题翻译：用于检测亚甲基四氢叶酸还原酶（MTHFR）基因型的核苷酸引物组和核苷酸探针
公开(公告)号：US07728119B2
公开(公告)日：2010-06-01
申请号：US12015645
申请日：2008-01-17
申请人： Naoko Nakamura , Keiko Ito , Masayoshi Takahashi , Koji Hashimoto , Nobuhiro Gemma
发明人： Naoko Nakamura , Keiko Ito , Masayoshi Takahashi , Koji Hashimoto , Nobuhiro Gemma
IPC分类号： C07H21/02 , C12Q1/68
CPC分类号： C12Q1/6827 , C12Q2537/137 , C12Q2525/301
摘要： There is provided is a nucleotide primer set for LAMP amplification used for detecting genotypes of single-nucleotide polymorphisms C677T and A1298C of an MTHFR gene. There is also provided a nucleotide probe for detecting an amplification product amplified by the primer set according to the present invention. There is also provided a method of detecting the genotypes of the single-nucleotide polymorphisms C677T and A1298C in the MTHFR gene, by using the primer set according to the present invention.
摘要翻译：提供了用于检测MTHFR基因的单核苷酸多态性C677T和A1298C的基因型的LAMP扩增的核苷酸引物组。还提供了用于检测根据本发明的引物组扩增的扩增产物的核苷酸探针。还提供了通过使用根据本发明的引物组来检测MTHFR基因中单核苷酸多态性C677T和A1298C的基因型的方法。

5. 发明申请

US20090075275A1 NUCLEIC ACID PROBE-IMMOBILIZED SUBSTRATE AND METHOD OF DETECTING THE PRESENCE OF TARGET NUCLEIC ACID BY USING THE SAME 审中-公开
标题翻译：核酸探针固定基质及其检测目标核酸存在的方法
公开(公告)号：US20090075275A1
公开(公告)日：2009-03-19
申请号：US12142230
申请日：2008-06-19
申请人： Masayoshi Takahashi , Jun Okada , Koji Hashimoto
发明人： Masayoshi Takahashi , Jun Okada , Koji Hashimoto
IPC分类号： C12Q1/68 , G01N33/50
CPC分类号： C12Q1/6837 , Y10T436/143333 , C12Q2525/197 , C12Q2525/161 , C12Q2563/107 , C12Q2563/113
摘要： The invention provides a nucleic acid probe-immobilized substrate comprising a substrate and a nucleic acid probe containing a nucleotide sequence complementary to a target sequence and immobilized via a spacer onto the substrate, wherein upon hybridization, with the nucleic acid probe, of a target nucleic acid partially containing the target sequence, the spacer satisfies the relationship: X≧Y wherein X is the length of the spacer and Y is the length of the target nucleic acid ranging from the end of the hybridized site at the side of the substrate to the end of the target nucleic acid at the side of the substrate.
摘要翻译：本发明提供了一种固定有核酸探针的底物，其包含底物和含有与靶序列互补的核苷酸序列并通过间隔基固定在底物上的核酸探针，其中在与核酸探针杂交时，靶核酸部分含有目标序列的酸，间隔物满足以下关系：<？in-line-formula description =“In-line formula”end =“lead”？> X> = Y <？in-line-formula description =“In 线式“end =”tail“？>其中X是间隔物的长度，Y是靶核酸的范围从底物侧面的杂交位点到靶核酸末端的长度酸在基材的一侧。

6. 发明申请

US20070007148A1 Base sequence detecting electrode, base sequence detecting device and base sequence detecting method 有权
标题翻译：碱基序列检测电极，碱基序列检测装置和碱基序列检测方法
公开(公告)号：US20070007148A1
公开(公告)日：2007-01-11
申请号：US11452370
申请日：2006-06-14
申请人： Jun Okada , Koji Hashimoto , Masayoshi Takahashi
发明人： Jun Okada , Koji Hashimoto , Masayoshi Takahashi
IPC分类号： G01N27/26
CPC分类号： C12Q1/6825 , C12Q2563/173 , C12Q2549/125
摘要： A conductive detecting electrode (2), first blocking molecules (21) formed so as to cover a surface of the detecting electrode (2), the first blocking molecules decreasing adsorption of an intercalating agent to the surface of the detecting electrode (2), a target-complementary probe (23) immobilized to the detecting electrode (2) via a spacer member (22) comprising straight chain organic molecules, the target-complementary probe including a base sequence complementary to a target base sequence which is an object of detection, a conductive comparison electrode (3), and second blocking molecules (31) formed so as to cover a surface of the comparison electrode (3), the second blocking molecules decreasing adsorption of an intercalating agent to the surface of the comparison electrode (3), are provided.
摘要翻译：导电检测电极（2），形成为覆盖检测电极（2）的表面的第一阻挡分子（21），第一阻挡分子减少插入剂吸附到检测电极（2）的表面，通过包含直链有机分子的间隔构件（22）固定在检测电极（2）上的目标互补探针（23），靶互补探针包括与作为检测对象的目标碱基序列互补的碱基序列，导电比较电极（3）和形成为覆盖比较电极（3）的表面的第二阻挡分子（31），第二阻断分子减少插入剂吸附到比较电极（3）的表面）。

7. 发明授权

US07718048B2 Base sequence detecting electrode, base sequence detecting device and base sequence detecting method 有权
标题翻译：碱基序列检测电极，碱基序列检测装置和碱基序列检测方法
公开(公告)号：US07718048B2
公开(公告)日：2010-05-18
申请号：US11452370
申请日：2006-06-14
申请人： Jun Okada , Koji Hashimoto , Masayoshi Takahashi
发明人： Jun Okada , Koji Hashimoto , Masayoshi Takahashi
IPC分类号： G01N27/327
CPC分类号： C12Q1/6825 , C12Q2563/173 , C12Q2549/125
摘要： A conductive detecting electrode (2), first blocking molecules (21) formed so as to cover a surface of the detecting electrode (2), the first blocking molecules decreasing adsorption of an intercalating agent to the surface of the detecting electrode (2), a target-complementary probe (23) immobilized to the detecting electrode (2) via a spacer member (22) comprising straight chain organic molecules, the target-complementary probe including a base sequence complementary to a target base sequence which is an object of detection, a conductive comparison electrode (3), and second blocking molecules (31) formed so as to cover a surface of the comparison electrode (3), the second blocking molecules decreasing adsorption of an intercalating agent to the surface of the comparison electrode (3), are provided.
摘要翻译：导电检测电极（2），形成为覆盖检测电极（2）的表面的第一阻挡分子（21），第一阻挡分子减少插入剂吸附到检测电极（2）的表面，通过包含直链有机分子的间隔构件（22）固定在检测电极（2）上的目标互补探针（23），靶互补探针包括与作为检测对象的目标碱基序列互补的碱基序列，导电比较电极（3）和形成为覆盖比较电极（3）的表面的第二阻挡分子（31），第二阻断分子减少插入剂吸附到比较电极（3）的表面）。

8. 发明申请

US20090061433A1 NUCLEOTIDE PRIMER SET AND NUCLEOTIDE PROBE FOR DETECTING GENOTYPE OF SERUM AMYLOID A1(SAA1) 审中-公开
标题翻译：用于检测血清AMYLOID A1（SAA1）基因组的核酸酶基因组和核酸探针
公开(公告)号：US20090061433A1
公开(公告)日：2009-03-05
申请号：US12049475
申请日：2008-03-17
申请人： Naoko NAKAMURA , Keiko Ito , Masayoshi Takahashi , Koji Hashimoto , Nobuhiro Gemma
发明人： Naoko NAKAMURA , Keiko Ito , Masayoshi Takahashi , Koji Hashimoto , Nobuhiro Gemma
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6853 , C12Q2531/119 , C12Q2525/155
摘要： Provided is a LAMP-amplification nucleotide primer set for detection of the genotype of single nucleotide polymorphisms C-13T, C2995T and T3010C of the SAA1 gene. Also provided is a nucleotide probe for detection of the amplification product amplified with the primer set according to the present invention. Further provided is a method of detecting the genotype of the single nucleotide polymorphisms C-13T, C2995T and T3010C of the SAA1 gene by using the primer set according to the present invention.
摘要翻译：提供了用于检测SAA1基因的单核苷酸多态性C-13T，C2995T和T3010C的基因型的LAMP扩增核苷酸引物组。还提供了用于检测用根据本发明的引物组扩增的扩增产物的核苷酸探针。进一步提供了通过使用本发明的引物组检测SAA1基因的单核苷酸多态性C-13T，C2995T和T3010C的基因型的方法。

9. 发明授权

US07097751B2 Base sequence detecting electrode, base sequence detecting device and base sequence detecting method 有权
标题翻译：碱基序列检测电极，碱基序列检测装置和碱基序列检测方法
公开(公告)号：US07097751B2
公开(公告)日：2006-08-29
申请号：US10239175
申请日：2002-08-28
申请人： Jun Okada , Koji Hashimoto , Masayoshi Takahashi
发明人： Jun Okada , Koji Hashimoto , Masayoshi Takahashi
IPC分类号： G01N27/327 , C12Q1/68
CPC分类号： C12Q1/6825 , C12Q2563/173 , C12Q2549/125
摘要： A conductive detecting electrode (2), first blocking molecules (21) formed so as to cover a surface of the detecting electrode (2), the first blocking molecules decreasing adsorption of an intercalating agent to the surface of the detecting electrode (2), a target-complementary probe (23) immobilized to the detecting electrode (2) via a spacer member (22) comprising straight chain organic molecules, the target-complementary probe including a base sequence complementary to a target base sequence which is an object of detection, a conductive comparison electrode (3), and second blocking molecules (31) formed so as to cover a surface of the comparison electrode (3), the second blocking molecules decreasing adsorption of an intercalating agent to the surface of the comparison electrode (3), are provided.
摘要翻译：导电检测电极（2），形成为覆盖检测电极（2）的表面的第一阻挡分子（21），第一阻挡分子减少插入剂吸附到检测电极（2）的表面，通过包含直链有机分子的间隔构件（22）固定在检测电极（2）上的目标互补探针（23），靶互补探针包括与作为检测对象的目标碱基序列互补的碱基序列，导电比较电极（3）和形成为覆盖比较电极（3）的表面的第二阻挡分子（31），第二阻断分子减少插入剂吸附到比较电极（3）的表面）。

10. 发明申请

US20050164286A1 Nucleic acid concentration quantitative analysis chip, nucleic acid concentration quantitative analysis apparatus, and nucleic acid concentration quantitative analysis method 审中-公开
标题翻译：核酸浓度定量分析芯片，核酸浓度定量分析仪器和核酸浓度定量分析方法
公开(公告)号：US20050164286A1
公开(公告)日：2005-07-28
申请号：US11082877
申请日：2005-03-18
申请人： Shin-ichi O'uchi , Hideyuki Funaki , Sadato Hongo , Koji Hashimoto , Nobuhiro Gemma , Jun Okada , Masayoshi Takahashi
发明人： Shin-ichi O'uchi , Hideyuki Funaki , Sadato Hongo , Koji Hashimoto , Nobuhiro Gemma , Jun Okada , Masayoshi Takahashi
IPC分类号： G01N33/53 , B01L3/00 , C12M1/00 , C12M1/34 , C12N15/09 , C12Q1/68 , C40B40/06 , G01N27/327 , G01N27/416 , G01N27/49 , G01N33/566 , G01N37/00
CPC分类号： C12M1/34 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00653 , B01J2219/00657 , B01J2219/00659 , B01J2219/00677 , B01J2219/00691 , B01J2219/00722 , B01L3/502715 , B01L2200/143 , B01L2200/148 , B01L2300/024 , B01L2300/0636 , B01L2300/0645 , B01L2300/087 , B01L2300/0883 , B01L2400/0487 , C12Q1/68 , C12Q1/6837 , C40B40/06
摘要： The present invention includes a plurality of working electrodes on which the same type of nucleic acid probes each having a nucleic acid complementary to a target nucleic acid are immobilized and which have different sensor areas and a normalization circuit which normalizes detection signals obtained by the working electrodes with respect to the respective sensor areas.
摘要翻译：本发明包括多个工作电极，其上固定有与靶核酸互补的核酸的相同类型的核酸探针，并且具有不同的传感器区域，以及归一化电路，其对由工作电极获得的检测信号进行归一化相对于各个传感器区域。

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