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    • 8. 发明授权
    • Primary cultured adipocytes for gene therapy
    • 原代培养的脂肪细胞进行基因治疗
    • US08071085B2
    • 2011-12-06
    • US12175186
    • 2008-07-17
    • Masashi ItoYasushi Saito
    • Masashi ItoYasushi Saito
    • A61K48/00C12P21/00C12P21/02
    • C12N5/0653A61K35/12C12N2510/00C12N2510/02
    • The present invention relates to primary cultured adipocytes for gene therapy, where the adipocytes stably maintain a foreign gene encoding a protein that is secreted outside of cells. This invention provides cells suitable for gene therapy, which can replace bone marrow cells and liver cells used for conventional ex vivo gene therapy. The present invention established methods for transferring foreign genes into primary cultured adipocytes, which are suitable for ex vivo gene therapy; can be easily collected and implanted; and can be removed after implantation. Specifically, the present invention established these methods that use retroviral vectors. The present invention also established primary cultured adipocytes for gene therapy, where the adipocytes stably maintain a foreign gene encoding a protein that is secreted outside of cells.
    • 本发明涉及用于基因治疗的原代培养的脂肪细胞,其中脂肪细胞稳定地维持编码在细胞外分泌的蛋白质的外源基因。 本发明提供适用于基因治疗的细胞,其可代替用于常规体外基因治疗的骨髓细胞和肝细胞。 本发明建立了将外源基因转移到适合离体基因治疗的原代培养脂肪细胞中的方法; 可以轻松收集和植入; 并且可以在植入后去除。 具体地,本发明建立了使用逆转录病毒载体的这些方法。 本发明还建立了用于基因治疗的原代培养的脂肪细胞,其中脂肪细胞稳定地保持编码在细胞外分泌的蛋白质的外源基因。