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    • 6. 发明授权
    • Endoglucanases
    • 内切葡聚糖酶
    • US06387690B1
    • 2002-05-14
    • US09229911
    • 1999-01-13
    • Martin SchüleinLene Nonboe AndersenSøren Flensted LassenMarkus Sakari KauppinenLene LangeRuby Ilum NielsenMichiko IharaShinobu Takagi
    • Martin SchüleinLene Nonboe AndersenSøren Flensted LassenMarkus Sakari KauppinenLene LangeRuby Ilum NielsenMichiko IharaShinobu Takagi
    • D06M1600
    • C12N9/2437A61K38/00C05F11/08C05F17/0036C07K2319/00C11D3/386C12P19/14C12Y302/01004D06M16/003D06P5/02D06P5/158D21C5/005D21H17/005D21H21/10Y02P20/145Y02W30/43
    • The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence consisting of 14 amino acid residues having the following sequence Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa Xaa Cys Xaa 1   2   3   4   5   6   7   8   9   10  11  12 Trp Xaa 13  14 and a second amino acid sequence consisting of 5 amino acid residues having the following sequence Trp Cys Cys Xaa Cys 1   2   3   4   5 wherein, in position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; in position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; in position 8 of the first sequence, the amino acid is Arg, Lys or His; in position 9, 10, 12 and 14, respectively, of the first sequence, and in position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue in position 12 is Ser, then the amino acid residue in position 14 is not Ser, and (ii) when the amino residue in position 12 is Gly, then the amino acid residue in position 14 is not Ala, performs very good in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.
    • 本发明涉及酶制剂,其基本上由具有纤维素分解活性的酶组成,并且包含由具有以下序列的14个氨基酸残基组成的第一氨基酸序列,以及由具有以下序列的5个氨基酸残基组成的第二个氨基酸序列,其中, 第一序列的第3位,氨基酸为Trp,Tyr或Phe; 在第一序列的位置4,氨基酸是Trp,Tyr或Phe; 在第一序列的位置8,氨基酸是Arg,Lys或His; 分别位于第一序列的第9,10,12和14位,在第二序列的位置4中,氨基酸是20个天然存在的氨基酸残基中的任一个,条件是在第一个氨基酸序列 ,(i)当位置12的氨基残基为Ser时,14位的氨基酸残基不为Ser,(ii)当位置12的氨基残基为Gly时,位置14的氨基酸残基为 Ala在工业应用中表现非常好,例如洗衣组合物,用于生物抛光新制造的纺织品,用于提供纤维素织物或服装的磨损外观以及纸浆处理。 此外,本发明涉及编码这些酶的DNA构建体,提供编码这种酶的基因的方法,生产酶的方法,含有这些酶的酶制剂,以及这些酶用于许多工业应用的用途。