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1. 发明授权

US08969087B2 Method of pre-treatment and staining of a biological sample and device for support of a biological sample and methods of using such device 有权
标题翻译：用于支持生物样品的生物样品和装置的预处理和染色方法以及使用这种装置的方法
公开(公告)号：US08969087B2
公开(公告)日：2015-03-03
申请号：US13605228
申请日：2012-09-06
申请人： Loren L. Bland , Marc E. Key , Lars Winther , David A. Stanforth , Martin N. Lindberg
发明人： Loren L. Bland , Marc E. Key , Lars Winther , David A. Stanforth , Martin N. Lindberg
IPC分类号： G01N33/00 , G01N1/30 , B01L3/00 , G01N1/31 , G01N35/00
CPC分类号： G01N1/30 , B01L3/545 , B01L2300/023 , B01L2300/024 , B01L2300/0822 , G01N1/312 , G01N35/00732 , G01N2035/00782 , Y10T436/255
摘要： There is disclosed a method of pre-treatment and staining, according to a protocol, of a biological sample disposed upon the surface of a carrier, the method comprising the step of recording at least one parameter relating to at least one protocol step in a non-volatile memory located either upon or within the carrier or a device incorporating the carrier. Also disclosed is a device comprising: a non-volatile memory; a surface of the device adapted to carry a biological sample; and communications means electrically coupled to the memory for enabling data transmission to or from an external apparatus. Also disclosed is a method of controlling processing of a biological sample disposed upon a carrier, comprising: providing, upon or within the carrier or an apparatus holding the carrier, a non-volatile memory having information relating to sample processing priority or protocol; reading the information; and scheduling the processing based upon the information.
摘要翻译：公开了根据方案对设置在载体表面上的生物样品进行预处理和染色的方法，所述方法包括以下步骤：将与至少一个方案步骤相关的至少一个参数记录在非 - 位于载体上或载体内的易失性存储器或包含载体的装置。还公开了一种装置，包括：非易失性存储器; 该装置的表面适于携带生物样品; 以及电连接到存储器的通信装置，用于使能到外部设备的数据传输。还公开了一种控制设置在载体上的生物样品的处理的方法，包括：在载体或保持载体的装置的上方或内部提供具有与样品处理优先级或方案有关的信息的非易失性存储器; 阅读信息; 并基于该信息调度处理。

2. 发明授权

US08283176B2 Method of pre-treatment and staining of a biological sample and device for support of a biological sample and methods of using such device 有权
标题翻译：用于支持生物样品的生物样品和装置的预处理和染色方法以及使用这种装置的方法
公开(公告)号：US08283176B2
公开(公告)日：2012-10-09
申请号：US11168987
申请日：2005-06-28
申请人： Loren L. Bland , Marc E. Key , Lars Winther , David A. Stanforth , Martin N. Lindberg
发明人： Loren L. Bland , Marc E. Key , Lars Winther , David A. Stanforth , Martin N. Lindberg
IPC分类号： G01N33/00
CPC分类号： G01N1/30 , B01L3/545 , B01L2300/023 , B01L2300/024 , B01L2300/0822 , G01N1/312 , G01N35/00732 , G01N2035/00782 , Y10T436/255
摘要： There is disclosed a method of pre-treatment and staining, according to a protocol, of a biological sample disposed upon the surface of a carrier, the method comprising the step of recording at least one parameter relating to at least one protocol step in a non-volatile memory located either upon or within the carrier or a device incorporating the carrier. Also disclosed is a device comprising: a non-volatile memory; a surface of the device adapted to carry a biological sample; and communications means electrically coupled to the memory for enabling data transmission to or from an external apparatus. Also disclosed is a method of controlling processing of a biological sample disposed upon a carrier, comprising: providing, upon or within the carrier or an apparatus holding the carrier, a non-volatile memory having information relating to sample processing priority or protocol; reading the information; and scheduling the processing based upon the information.
摘要翻译：公开了根据方案对设置在载体表面上的生物样品进行预处理和染色的方法，所述方法包括以下步骤：将与至少一个方案步骤相关的至少一个参数记录在非 - 位于载体上或载体内的易失性存储器或包含载体的装置。还公开了一种装置，包括：非易失性存储器; 所述装置的表面适于携带生物样品; 以及电连接到存储器的通信装置，用于使能到外部设备的数据传输。还公开了一种控制设置在载体上的生物样品的处理的方法，包括：在载体或保持载体的装置的上方或内部提供具有与样品处理优先级或方案有关的信息的非易失性存储器; 阅读信息; 并基于该信息调度处理。

3. 发明授权

US07875245B2 Method and apparatus for automated pre-treatment and processing of biological samples 有权
标题翻译：用于生物样品自动预处理和处理的方法和装置
公开(公告)号：US07875245B2
公开(公告)日：2011-01-25
申请号：US11156760
申请日：2005-06-20
申请人： John A. Favuzzi , Kristopher S. Buchanan , Michael T. Barber , Loren L. Bland , Dawn M. Rohrbacker , Robert M. Clark , Gordon A. Feingold , Benno M. Guggenheimer , Marc E. Key , Merritt M. Martin, Jr. , Robert L. Lathrop , Jeffery L. Rau , Jonathan R. Sweda , Douglas F. Sweet , John E. Van Bosch , Lars Winther , Rosanne Welcher
发明人： John A. Favuzzi , Kristopher S. Buchanan , Michael T. Barber , Loren L. Bland , Dawn M. Rohrbacker , Robert M. Clark , Gordon A. Feingold , Benno M. Guggenheimer , Marc E. Key , Merritt M. Martin, Jr. , Robert L. Lathrop , Jeffery L. Rau , Jonathan R. Sweda , Douglas F. Sweet , John E. Van Bosch , Lars Winther , Rosanne Welcher
IPC分类号： B01L3/02
CPC分类号： G01N35/0092 , B01L3/508 , B01L7/00 , B01L9/06 , B01L2200/025 , B01L2200/16 , B01L2300/021 , B01L2300/0822 , B01L2300/1822 , G01N1/312 , G01N35/00029 , G01N35/00871 , G01N35/0095 , G01N35/0099 , G01N35/1002 , G01N35/1004 , G01N35/1011 , G01N2035/00356 , G01N2035/00366 , G01N2035/00445 , G01N2035/00455 , G01N2035/00534 , G01N2035/00633 , G01N2035/00673 , G01N2035/00752 , G01N2035/00861 , G01N2035/00881 , G01N2035/102 , G01N2035/1058 , Y02A90/26 , Y10T436/11 , Y10T436/112499 , Y10T436/12 , Y10T436/2575
摘要： The present invention concerns a method and apparatus for automatic processing a biological sample on a carrier, perhaps robotically, by applying predetermined amounts of reagents in a predetermined sequence according to a processing protocol, the processing including pre-treatment steps, under the control of an adaptive processing control system using a sample process parameter input that may be independent and an independent process parameter memory that does not interrupt process operation when being used, such that samples may be added or removed without interrupting the processing of other samples. Also included is an image capture function for sample and reagent identification and process monitoring, as well as temperature regulation and environmental control functions.
摘要翻译：本发明涉及一种用于通过根据处理方案以预定顺序施加预定量的试剂来自动地处理载体上的生物样品的方法和装置，所述方法和装置可以机器人地处理，所述处理包括预处理步骤，在自适应处理控制系统使用可以是独立的样本过程参数输入和在使用时不中断处理操作的独立过程参数存储器，使得可以添加或移除样本而不中断其他样本的处理。还包括用于样品和试剂识别和过程监测以及温度调节和环境控制功能的图像捕获功能。

4. 发明授权

US07867443B2 Method and apparatus for automated pre-treatment and processing of biological samples 有权
标题翻译：用于生物样品自动预处理和处理的方法和装置
公开(公告)号：US07867443B2
公开(公告)日：2011-01-11
申请号：US11176757
申请日：2005-07-07
申请人： Marc E. Key , Scott A. Devore , Loren L. Bland , Uffe Lovborg
发明人： Marc E. Key , Scott A. Devore , Loren L. Bland , Uffe Lovborg
IPC分类号： G01N21/00
CPC分类号： G01N33/5306 , G01N1/30 , G01N33/54393 , Y10T436/11 , Y10T436/111666 , Y10T436/2575
摘要： The present invention concerns a method and apparatus for automatic staining or other processing of a biological sample on a slide, perhaps robotically, by applying predetermined amounts of reagents in a predetermined sequence according to a processing protocol, the processing including pre-treatment steps, under the control of an adaptive processing control system. Further provided is a method of antigen retrieval, the method comprising contacting, within a heated processing tank of an automated staining apparatus, at least one biological sample with at least one composition for antigen retrieval comprising at least one chelating agent, at least one detergent and at least 50% by volume of at least one liquid that facilitates antigen retrieval.
摘要翻译：本发明涉及一种用于通过根据处理方案以预定顺序施加预定量的试剂，可能机器人地在载玻片上自动染色或其它处理生物样品的方法和装置，该处理包括预处理步骤自适应处理控制系统的控制。进一步提供的是一种抗原回收方法，该方法包括在加热处理罐内自动染色装置的至少一个生物样品与至少一种用于抗原回收的组合物接触，所述组合物用于抗原回收，包括至少一种螯合剂，至少一种洗涤剂和至少50体积％的至少一种促进抗原回收的液体。

5. 发明授权

US09182324B2 Systems and methods for the automated pre-treatment and processing of biological samples 有权
标题翻译：用于生物样品自动预处理和处理的系统和方法
公开(公告)号：US09182324B2
公开(公告)日：2015-11-10
申请号：US13345605
申请日：2012-01-06
申请人： Gordon Alan Feingold , James B. Gilmartin , Mark Richard Holbrook , John A. Favuzzi , Marc E. Key
发明人： Gordon Alan Feingold , James B. Gilmartin , Mark Richard Holbrook , John A. Favuzzi , Marc E. Key
IPC分类号： G01N21/00 , G01N31/00 , G01N33/00 , G01N1/31 , G01N35/00 , G06F19/00
CPC分类号： G01N1/312 , G01N35/0092 , G01N35/0099 , G06F19/00 , G16H40/63 , Y10T436/11 , Y10T436/110833 , Y10T436/111666 , Y10T436/112499 , Y10T436/114998 , Y10T436/25 , Y10T436/2575
摘要： Systems and methods in accordance with embodiments of the present invention allow for the automatic control and scheduling of a staining apparatus for biological samples on slides present within the apparatus. In some embodiments, the actions of a robot coupled to the staining apparatus, which performs some of the staining tasks on the individual slides in accordance with their respective protocols, may be prioritized and scheduled. In some embodiments, the scheduling may result in increasing or maximizing the throughput of slides. In some embodiments, robot scheduling ensures that the individual slides are processed substantially within the tolerances specified by their respective protocols. In some embodiments, the robot scheduler may respond to spontaneous user actions and adaptively schedule or re-schedule robot actions.
摘要翻译：根据本发明的实施例的系统和方法允许对存在于装置内的载玻片上的生物样品的染色装置进行自动控制和调度。在一些实施例中，可以对与染色设备耦合的机器人的动作进行优先级排列，所述机器人根据各自的协议在各个幻灯片上执行一些染色任务。在一些实施例中，调度可导致增加或最大化幻灯片的吞吐量。在一些实施例中，机器人调度确保各个滑块基本上在其各自协议规定的公差内被处理。在一些实施例中，机器人调度器可以响应自发的用户动作并且自适应地调度或重新调度机器人动作。

6. 发明授权

US07584019B2 Systems and methods for the automated pre-treatment and processing of biological samples 有权
标题翻译：用于生物样品自动预处理和处理的系统和方法
公开(公告)号：US07584019B2
公开(公告)日：2009-09-01
申请号：US11229098
申请日：2005-09-16
申请人： Gordon Alan Feingold , James B. Gilmartin , Mark Richard Holbrook , John A. Favuzzi , Marc E. Key
发明人： Gordon Alan Feingold , James B. Gilmartin , Mark Richard Holbrook , John A. Favuzzi , Marc E. Key
IPC分类号： G06F19/00
CPC分类号： G01N1/312 , G01N35/0092 , G01N35/0099 , G06F19/00 , G16H40/63 , Y10T436/11 , Y10T436/110833 , Y10T436/111666 , Y10T436/112499 , Y10T436/114998 , Y10T436/25 , Y10T436/2575
摘要： Systems and methods allowing for the automatic control and scheduling of a staining apparatus for biological samples on slides present within the apparatus. In some embodiments, the actions of a robot coupled to the staining apparatus, which performs some of the staining tasks on the individual slides in accordance with their respective protocols, may be prioritized and scheduled. In some embodiments, the scheduling may result in increasing or maximizing the throughput of slides. In some embodiments, robot scheduling ensures that the individual slides are processed substantially within the tolerances specified by their respective protocols. In some embodiments, the robot scheduler may respond to spontaneous user actions and adaptively schedule or re-schedule robot actions.
摘要翻译：允许在装置内存在的载玻片上的生物样品的染色装置的自动控制和调度的系统和方法。在一些实施例中，可以对与染色设备耦合的机器人的动作进行优先级排列，所述机器人根据各自的协议在各个幻灯片上执行一些染色任务。在一些实施例中，调度可导致增加或最大化幻灯片的吞吐量。在一些实施例中，机器人调度确保各个滑块基本上在其各自协议规定的公差内被处理。在一些实施例中，机器人调度器可以响应自发的用户动作并且自适应地调度或重新调度机器人动作。

7. 发明授权

US07603201B2 Systems and methods for the automated pre-treatment and processing of biological samples 有权
标题翻译：用于生物样品自动预处理和处理的系统和方法
公开(公告)号：US07603201B2
公开(公告)日：2009-10-13
申请号：US11338524
申请日：2006-01-23
申请人： Gordon Alan Feingold , James B. Gilmartin , Mark Richard Holbrook , John A. Favuzzi , Marc E. Key
发明人： Gordon Alan Feingold , James B. Gilmartin , Mark Richard Holbrook , John A. Favuzzi , Marc E. Key
IPC分类号： G06F19/00
CPC分类号： G01N1/312 , G01N35/0092 , G01N35/0099 , G06F19/00 , G16H40/63 , Y10T436/11 , Y10T436/110833 , Y10T436/111666 , Y10T436/112499 , Y10T436/114998 , Y10T436/25 , Y10T436/2575
摘要： Systems and methods allowing for the automatic control and scheduling of a staining apparatus for biological samples on slides present within the apparatus. In some embodiments, the actions of a robot coupled to the staining apparatus, which performs some of the staining tasks on the individual slides in accordance with their respective protocols, may be prioritized and scheduled. In some embodiments, the scheduling may result in increasing or maximizing the throughput of slides. In some embodiments, robot scheduling ensures that the individual slides are processed substantially within the tolerances specified by their respective protocols. In some embodiments, the robot scheduler may respond to spontaneous user actions and adaptively schedule or re-schedule robot actions.
摘要翻译：公开了允许在装置内存在的载玻片上的生物样品的染色装置的自动控制和调度的系统和方法。在一些实施例中，可以对与染色设备耦合的机器人的动作进行优先级排列，所述机器人根据各自的协议在各个幻灯片上执行一些染色任务。在一些实施例中，调度可导致增加或最大化幻灯片的吞吐量。在一些实施例中，机器人调度确保各个滑块基本上在其各自协议规定的公差内被处理。在一些实施例中，机器人调度器可以响应自发的用户动作并且自适应地调度或重新调度机器人动作。

8. 发明授权

US5244787A Antigen retrieval in formalin-fixed tissues using microwave energy 失效
标题翻译：使用微波能量在福尔马林固定组织中的抗原检索
公开(公告)号：US5244787A
公开(公告)日：1993-09-14
申请号：US649036
申请日：1991-01-31
申请人： Marc E. Key , Shan-Rong Shi , Krishan L. Kalra
发明人： Marc E. Key , Shan-Rong Shi , Krishan L. Kalra
IPC分类号： G01N1/30 , G01N1/44 , G01N33/58
CPC分类号： G01N1/44 , G01N33/581 , G01N1/30 , Y10S435/96
摘要： A method of immunologically staining a formalin-fixed tissue preparation, which comprises (a) subjecting a formalin-fixed tissue preparation to microwave energy while the tissue preparation is submersed in water for a time sufficient to increase immunostaining efficiency; (b) removing the tissue preparation from the water and cooling; and (c) contacting the tissue preparation with an immunological staining reagent.
摘要翻译：一种对福尔马林固定的组织制剂进行免疫染色的方法，其包括（a）将福尔马林固定的组织制剂在微波能量下进行，同时将组织制剂浸入水中足以提高免疫染色效率的时间; （b）从水中去除组织制剂并冷却; 和（c）使组织制剂与免疫染色试剂接触。

9. 发明授权

US4975377A Cell growth chambers and method of use thereof 失效
标题翻译：细胞生长室及其使用方法
公开(公告)号：US4975377A
公开(公告)日：1990-12-04
申请号：US50510
申请日：1987-05-14
申请人： Marc E. Key
发明人： Marc E. Key
IPC分类号： C12M1/20 , C12M3/00
CPC分类号： C12M23/20 , C12M23/10 , C12M25/14
摘要： Growth chambers for anchorage-independent cell growth therein are formed of a gel matrix having a surface disallowing anchorage-dependent cell growth over the full interior thereof. In a preferred form the chambers have a generally cylidnrical wall and an integral convex bottom wall forming an annular volume at the foot of the cylindrical wall which is substantially lower than the central portion to concentrate such anchorage-indpendent cells. The gel matrix is sufficiently permeable to permit passage of cell-growth nutrients and waste product solutes through said wall when the chambers are filled below the open end and submerged in a growth medium. Preferably the gel matrix is formed of 1% to 5% cross-linked polyacrylamide and from 99% to 95% water.In a preferred method of using the growth chambers, undifferentiated tumor cells and normal cells, including fibroblasts, are cultured together. Anchorage-independent tumor cells proliferate while anchorage-dependent cells are unable to grow without attachment. The method is useful for evaluating in vitro therapeutic agents to control tumor growth, normal cell growth or microspheres and generation of immunoglobulins from lymphocyte cells.
摘要翻译：用于与其无关的细胞生长的生长室由具有在其整个内部不允许锚定依赖性细胞生长的表面的凝胶基质形成。在优选形式中，室具有大致圆形的壁和整体的凸底壁，其在圆柱形壁的底部形成基本上低于中心部分的环形体积，以集中这种固定型静电池。凝胶基质足够透气，允许细胞生长营养物质和废产物溶质通过所述壁，当室被填充在开口端下方并浸没在生长培养基中时。优选地，凝胶基质由1％至5％的交联聚丙烯酰胺和99％至95％的水形成。在使用生长室的优选方法中，将未分化的肿瘤细胞和包括成纤维细胞的正常细胞一起培养。不依赖锚定依赖的细胞不能生长而不依赖于锚定的肿瘤细胞增殖。该方法可用于评估体外治疗剂以控制肿瘤生长，正常细胞生长或微球体以及从淋巴细胞细胞产生免疫球蛋白。

10. 发明申请

US20100330592A1 METHOD FOR DETECTING TRUNCATED MOLECULES 审中-公开
标题翻译：检测截短分子的方法
公开(公告)号：US20100330592A1
公开(公告)日：2010-12-30
申请号：US12865087
申请日：2009-01-29
申请人： Marc E. Key
发明人： Marc E. Key
IPC分类号： G01N33/53
CPC分类号： G01N33/6878 , G01N33/74 , G01N2333/485 , G01N2333/71
摘要： Exemplary disclosed embodiments may comprise, for example, providing a sample potentially comprising a native molecule and/or a truncated molecule. The native molecule comprises at least first and second regions recognized by first and second specific binding moieties, and the truncated molecule includes only one of the first and second regions. A composition comprising first and second specific binding moieties is applied to the sample in a manner effective to form first and second specific binding pairs with the first and second regions. For example, if the molecule is a protein, such as HER2, the protein may have a first epitope and a second epitope. Once a specific binding pair is formed, the pair must be visualized. Certain disclosed embodiments comprise a direct detection method whereby primary antibodies are coupled to signal generating moieties. Alternatively, signal amplification techniques can be used to visualize a specific binding pair.
摘要翻译：示例性的公开实施例可以包括例如提供潜在地包含天然分子和/或截短分子的样品。天然分子包含由第一和第二特异性结合部分识别的至少第一和第二区域，并且截短分子仅包括第一和第二区域中的一个。将包含第一和第二特异性结合部分的组合物以有效形成与第一和第二区域的第一和第二特异性结合对的方式施用于样品。例如，如果分子是诸如HER2的蛋白质，则蛋白质可以具有第一表位和第二表位。一旦形成特定的结合对，该对必须可视化。某些公开的实施方案包括直接检测方法，其中一抗被偶联到信号产生部分。或者，可以使用信号放大技术来可视化特异性结合对。

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