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1. 发明申请

US20070172937A1 Recombinant cells that highly express chromosomally-integrated heterologous genes 审中-公开
标题翻译：高度表达染色体整合的异源基因的重组细胞
公开(公告)号：US20070172937A1
公开(公告)日：2007-07-26
申请号：US11725728
申请日：2007-03-20
申请人： Lonnie Ingram , Kazuyoshi Ohta , Brent Wood
发明人： Lonnie Ingram , Kazuyoshi Ohta , Brent Wood
IPC分类号： C12N1/21 , C12N15/74
CPC分类号： C12N15/67 , C12N9/1029 , C12N15/74 , C12Y203/01054
摘要： Recombinant host cells are obtained that comprise (A) a heterologous, polypeptide-encoding polynucleotide segment, stably integrated into a chromosome, which is under transcriptional control of an endogenous promoter and (B) a mutation that effects increased expression of the heterologous segment, resulting in enhanced production by the host cells of each polypeptide encoded by that segment, relative to production of each polypeptide by the host cells in the absence of the mutation. The increased expression thus achieved is retained in the absence of conditions that select for cells displaying such increased expression. When the integrated segment comprises, for example, ethanol-production genes from an efficient ethanol producer like Zymomonas mobilis, recombinant Escherichia coli and other enteric bacterial cells within the present invention are capable of converting a wide range of biomass-derived sugars efficiently to ethanol.
摘要翻译：获得重组宿主细胞，其包含（A）编码染色体的异源，编码多肽的多核苷酸片段，其在内源性启动子的转录控制下（B）产生异源片段表达增加的突变，导致在不存在突变的情况下，通过宿主细胞增强由该片段编码的每种多肽的产生，相对于宿主细胞每种多肽的产生。在没有选择用于显示这种增加的表达的细胞的条件的情况下，保留了如此实现的增加的表达。当整合片段包括例如来自有效乙醇生产者的乙醇生产基因如运动发酵单胞菌，本发明中的重组大肠杆菌和其它肠道细菌细胞能够将大范围的生物质衍生的糖有效转化为乙醇。

2. 发明授权

US08700349B2 Method for measuring scattering absorber and device for measuring scattering absorber 有权
标题翻译：测量散射吸收体的方法和测量散射吸收体的装置
公开(公告)号：US08700349B2
公开(公告)日：2014-04-15
申请号：US13130117
申请日：2009-09-01
申请人： Kenji Yoshimoto , Kazuyoshi Ohta , Daisuke Yamashita , Hiroaki Suzuki
发明人： Kenji Yoshimoto , Kazuyoshi Ohta , Daisuke Yamashita , Hiroaki Suzuki
IPC分类号： G01R13/02 , G06F19/00 , A61B5/00 , G01N21/47
CPC分类号： A61B5/0091 , A61B5/0059 , A61B5/4312 , G01N21/4795
摘要： In a method for measuring a scattering medium, pulse light with a predetermined wavelength is made incident on a scattering medium which is a measurement object and a scattering medium for reference, the pulse light transmitted inside the scattering media is detected to acquire a light detection signal, the measurement waveform is acquired on the basis of the detected light detection signal, a parameter of a function showing the theoretical waveform of the measurement object is specified in such a manner that the result of convolution operation on the theoretical waveform of the measurement object and the measurement waveform of the reference is made equal to the result of convolution operation on the theoretical waveform of the reference and the measurement waveform of the measurement object, and calculation is made for the internal information of the scattering medium on the basis of the theoretical waveform shown by the function.
摘要翻译：在用于测量散射介质的方法中，将具有预定波长的脉冲光入射到作为测量对象的散射介质和用于参考的散射介质，检测在散射介质内透射的脉冲光以获取光检测信号基于检测到的光检测信号获取测量波形，表示测量对象的理论波形的功能的参数被指定为对测量对象的理论波形进行卷积运算的结果，以及使基准的测量波形等于基准的理论波形和测量对象的测量波形的卷积运算结果，并根据理论波形计算散射介质的内部信息由功能显示。

3. 发明授权

US6107093A Recombinant cells that highly express chromosomally-integrated heterologous genes 有权
标题翻译：高度表达染色体整合的异源基因的重组细胞
公开(公告)号：US6107093A
公开(公告)日：2000-08-22
申请号：US134403
申请日：1998-08-14
申请人： Lonnie O. Ingram , Kazuyoshi Ohta , Brent E. Wood
发明人： Lonnie O. Ingram , Kazuyoshi Ohta , Brent E. Wood
IPC分类号： C12N1/21 , C12N9/04 , C12N9/88 , C12N15/01 , C12N15/31 , C12N15/52 , C12N15/70 , C12N15/74 , C12N15/90 , C12P7/06 , C12P7/10 , G06T1/00 , H04N1/00
CPC分类号： C12N9/0006 , C12N15/01 , C12N15/52 , C12N15/70 , C12N15/74 , C12N15/902 , C12N9/88 , C12P7/065 , C12P7/10 , G06T1/00 , H04N1/00 , Y02E50/16 , Y02E50/17
摘要： Recombinant host cells are obtained that comprise (A) a heterologous, polypeptide-encoding polynucleotide segment, stably integrated into a chromosome, which is under transcriptional control of an endogenous promoter and (B) a mutation that effects increased expression of the heterologous segment, resulting in enhanced production by the host cells of each polypeptide encoded by that segment, relative to production of each polypeptide by the host cells in the absence of the mutation. The increased expression thus achieved is retained in the absence of conditions that select for cells displaying such increased expression. When the integrated segment comprises, for example, ethanol-production genes from an efficient ethanol producer like Zymomonas mobilis, recombinant Escherichia coli and other enteric bacterial cells within the present invention are capable of converting a wide range of biomass-derived sugars efficiently to ethanol.
摘要翻译：获得重组宿主细胞，其包含（A）编码染色体的异源，编码多肽的多核苷酸片段，其在内源性启动子的转录控制下（B）产生异源片段表达增加的突变，导致在不存在突变的情况下，通过宿主细胞增强由该片段编码的每种多肽的产生，相对于宿主细胞每种多肽的产生。在没有选择用于显示这种增加的表达的细胞的条件的情况下，保留了如此实现的增加的表达。当整合片段包括例如来自有效乙醇生产者的乙醇生产基因如运动发酵单胞菌，本发明中的重组大肠杆菌和其它肠道细菌细胞能够将大范围的生物质衍生的糖有效转化为乙醇。

4. 发明授权

US5919647A Methods and apparatuses for examining pathogen resistance of plant, for evaluating ability to impart pathogen resistance to plant, and for evaluating agricultural chemical 失效
标题翻译：用于检测植物病原体抗性的方法和装置，用于评估赋予植物病原体抗性的能力和用于评估农业化学品的能力
公开(公告)号：US5919647A
公开(公告)日：1999-07-06
申请号：US924119
申请日：1997-09-05
申请人： Mitsuo Hiramatsu , Kazuyoshi Ohta , Takahiro Makino , Kimihiko Kato , Sakio Suzuki, deceased
发明人： Mitsuo Hiramatsu , Kazuyoshi Ohta , Takahiro Makino , Kimihiko Kato , Sakio Suzuki, deceased
IPC分类号： G01N21/64 , C12Q1/02 , C12M1/34
CPC分类号： G01N21/64
摘要： An apparatus including: a sprayer for inoculating a microbe on a first division divided from at least one sample of a plant to be examined; sample leaving device for leaving a second division divided from the sample not inoculated with the microbe and the first division inoculated with the microbe, for a predetermined period of time under a predetermined condition; first and second photodetector for respectively measuring the quantities of luminescence emitted from the first and second divisions which have been left standing by the sample leaving device; and a computer for comparing the quantities of the luminescence measured by the first and second photodetector, thereby to examine the resistance or susceptibility of the plant to the microbe.
摘要翻译：一种装置，包括：用于将微生物从至少一个待检查植物样品划分的第一分区上接种的喷雾器; 样品离开装置，用于离开未接种微生物的样品和用微生物接种的第一分离物除以预定时间段预定条件下的第二分隔; 第一和第二光电检测器，用于分别测量由样品离开装置留置的第一和第二分区发射的发光量; 以及用于比较由第一和第二光电检测器测量的发光量的计算机，从而检查植物对微生物的抗性或易感性。

5. 发明授权

US06774341B2 Method of severing an optical fiber using a laser beam 失效
标题翻译：使用激光束切断光纤的方法
公开(公告)号：US06774341B2
公开(公告)日：2004-08-10
申请号：US10630859
申请日：2003-07-31
申请人： Kazuyoshi Ohta
发明人： Kazuyoshi Ohta
IPC分类号： B23K2638
CPC分类号： B23K26/38 , G02B6/25
摘要： A method of severing an optical fiber using a laser beam, in which a laser beam emitted from a laser beam source is applied through a square light transmitting section and a lens to an optical fiber, to form a square light spot on the irradiated portion, for melting, evaporating and severing the optical fiber at said irradiated portion, comprising the step of fixing the portion to be severed at, of the optical fiber, in a cylindrical capillary formed to be flat on the side to be irradiated with the laser beam.
摘要翻译：使用激光束切断光纤的方法，其中从激光束源发射的激光束通过方形透光部分和透镜施加到光纤，以在照射部分上形成方形光点，用于在所述照射部分处熔化，蒸发和切断光纤，包括将要在光纤上切断的部分固定在待激光束被照射的一侧上形成为平坦的圆柱形毛细管的步骤。

6. 发明授权

US6075610A Method and apparatus for measuring internal property distribution 失效
标题翻译：测量内部财产分配的方法和装置
公开(公告)号：US6075610A
公开(公告)日：2000-06-13
申请号：US853879
申请日：1997-05-09
申请人： Yukio Ueda , Yutaka Tsuchiya , Kazuyoshi Ohta
发明人： Yukio Ueda , Yutaka Tsuchiya , Kazuyoshi Ohta
IPC分类号： G01B11/00 , A61B10/00 , G01J1/00 , G01N21/17 , G01N21/49
CPC分类号： G01N21/49
摘要： A method for measuring an internal property distribution which includes making measurement light incident from a plurality of light incidence positions on a surface of a measured object, detecting the measurement light that has passed through the object, successively or simultaneously, at at least one light detection position out of a plurality of light detection positions on the surface of the object. A plurality of measurement values obtained by a plurality of combinations of the light incidence position and said light detection position are extracted. A mean value of the measured values are calculated to obtain a reference value and a change amount of a predetermined internal property in each of the plurality of regions of the object are calculated using the plurality of measured values obtained. The reference value thereby establishing an internal property change amount distribution in the object.
摘要翻译：一种内部性质分布的测定方法，其特征在于，包括：从测量对象的表面上的多个入射位置入射的测量光，连续或同时地以至少一个光检测方式检测已经通过所述物体的测量光位于物体表面上的多个光检测位置之外。提取通过光入射位置和所述光检测位置的多个组合获得的多个测量值。计算测量值的平均值以获得参考值，并且使用获得的多个测量值来计算物体的多个区域中的每一个中的预定内部特性的变化量。参考值由此建立对象内部属性变化量分布。

7. 发明申请

US20110224931A1 METHOD FOR MEASURING SCATTERING ABSORBER AND DEVICE FOR MEASURING SCATTERING ABSORBER 有权
标题翻译：用于测量散射吸收器的方法和用于测量散射吸收器的装置
公开(公告)号：US20110224931A1
公开(公告)日：2011-09-15
申请号：US13130117
申请日：2009-09-01
申请人： Kenji Yoshimoto , Kazuyoshi Ohta , Daisuke Yamashita , Hiroaki Suzuki
发明人： Kenji Yoshimoto , Kazuyoshi Ohta , Daisuke Yamashita , Hiroaki Suzuki
IPC分类号： G06F19/00
CPC分类号： A61B5/0091 , A61B5/0059 , A61B5/4312 , G01N21/4795
摘要： Acquired is the internal information of a scattering medium more accurately and easily, with the influence of an instrumental function taken into account, even where noise is contained in a measurement waveform.In a method for measuring a scattering medium, pulse light with a predetermined wavelength is made incident on a scattering medium which is a measurement object and a scattering medium for reference (S01b, S02b), the pulse light transmitted inside each of the scattering media is detected to acquire a light detection signal (S01c, S02c), the measurement waveform is acquired on the basis of the detected light detection signal (S01d, S02d), a parameter of a function showing the theoretical waveform of the measurement object is specified in such a manner that the result of convolution operation on the theoretical waveform of the measurement object and the measurement waveform of the reference is made equal to the result of convolution operation on the theoretical waveform of the reference and the measurement waveform of the measurement object (S03), and calculation is made for the internal information of the scattering medium on the basis of the theoretical waveform shown by the function (S04).
摘要翻译：即使在测量波形中包含噪声的情况下，也可以考虑到仪器功能的影响，更准确，更容易地获取散射介质的内部信息。在用于测量散射介质的方法中，将具有预定波长的脉冲光入射到作为测量对象的散射介质和用于参考的散射介质（S01b，S02b），在每个散射介质内传输的脉冲光为检测以获取光检测信号（S01c，S02c）时，基于检测到的光检测信号（S01d，S02d）获取测量波形，表示测量对象的理论波形的函数的参数被指定使对测量对象的理论波形的卷积运算结果和基准的测量波形的结果等于对基准的理论波形和测量对象的测量波形的卷积运算的结果（S03），并根据功能所示的理论波形对散射介质的内部信息进行计算（ S04）。

8. 发明授权

US07192772B1 Recombinant cells that highly express chromosomally-integrated heterologous gene 失效
标题翻译：高度表达染色体整合的异源基因的重组细胞
公开(公告)号：US07192772B1
公开(公告)日：2007-03-20
申请号：US09620060
申请日：2000-07-21
申请人： Lonnie O. Ingram , Kazuyoshi Ohta , Brent E. Wood
发明人： Lonnie O. Ingram , Kazuyoshi Ohta , Brent E. Wood
IPC分类号： C12N15/00 , C12N1/20 , C07H21/04
CPC分类号： C12N15/67 , C12N9/1029 , C12N15/74 , C12Y203/01054
摘要： Recombinant host cells are obtained that comprise (A) a heterologous, polypeptide-encoding polynucleotide segment, stably integrated into a chromosome, which is under transcriptional control of an endogenous promoter and (B) a mutation that effects increased expression of the heterologous segment, resulting in enhanced production by the host cells of each polypeptide encoded by that segment, relative to production of each polypeptide by the host cells in the absence of the mutation. The increased expression thus achieved is retained in the absence of conditions that select for cells displaying such increased expression. When the integrated segment comprises, for example, ethanol-production genes from an efficient ethanol producer like Zymomonas mobilis, recombinant Escherichia coli and other enteric bacterial cells within the present invention are capable of converting a wide range of biomass-derived sugars efficiently to ethanol.
摘要翻译：获得重组宿主细胞，其包含（A）编码染色体的异源，编码多肽的多核苷酸片段，其在内源性启动子的转录控制下（B）产生异源片段表达增加的突变，导致在不存在突变的情况下，通过宿主细胞增强由该片段编码的每种多肽的产生，相对于宿主细胞每种多肽的产生。在没有选择用于显示这种增加的表达的细胞的条件的情况下，保留了如此实现的增加的表达。当整合片段包括例如来自有效乙醇生产者的乙醇生产基因如运动发酵单胞菌，本发明中的重组大肠杆菌和其它肠道细菌细胞能够将大范围的生物质衍生的糖有效转化为乙醇。

9. 发明授权

US5821093A Recombinant cells that highly express chromosomally-integrated heterologous genes 失效
公开(公告)号：US5821093A
公开(公告)日：1998-10-13
申请号：US363868
申请日：1994-12-27
申请人： Lonnie O. Ingram , Kazuyoshi Ohta , Brent E. Wood
发明人： Lonnie O. Ingram , Kazuyoshi Ohta , Brent E. Wood
IPC分类号： C12N1/21 , C12N9/04 , C12N9/88 , C12N15/01 , C12N15/31 , C12N15/52 , C12N15/70 , C12N15/74 , C12N15/90 , C12P7/06 , C12P7/10 , G06T1/00 , H04N1/00 , C07H21/04 , C12N15/63
CPC分类号： C12N9/0006 , C12N15/01 , C12N15/52 , C12N15/70 , C12N15/74 , C12N15/902 , C12N9/88 , C12P7/065 , C12P7/10 , G06T1/00 , H04N1/00 , Y02E50/16 , Y02E50/17
摘要： Recombinant host cells are obtained that comprise (A) a heterologous, polypeptide-encoding polynucleotide segment, stably integrated into a chromosome, which is under transcriptional control of an endogenous promoter and (B) a mutation that effects increased expression of the heterologous segment, resulting in enhanced production by the host cells of each polypeptide encoded by that segment, relative to production of each polypeptide by the host cells in the absence of the mutation. The increased expression thus achieved is retained in the absence of conditions that select for cells displaying such increased expression. When the integrated segment comprises, for example, ethanol-production genes from an efficient ethanol producer like Zymomonas mobilis, recombinant Escherichia coli and other enteric bacterial cells within the present invention are capable of converting a wide range of biomass-derived sugars efficiently to ethanol.

10. 发明授权

US5640247A Method for measuring internal information in a scattering medium and apparatus for the same 失效
标题翻译：用于测量散射介质中的内部信息的方法及其装置
公开(公告)号：US5640247A
公开(公告)日：1997-06-17
申请号：US353202
申请日：1994-12-01
申请人： Yutaka Tsuchiya , Kazuyoshi Ohta , Tsuneyuki Urakami
发明人： Yutaka Tsuchiya , Kazuyoshi Ohta , Tsuneyuki Urakami
IPC分类号： G01N33/72 , A61B5/145 , A61B5/1455 , G01J1/00 , G01N21/17 , G01N21/49 , G01N21/47 , A61B5/00 , A61B6/00 , G01N21/00
CPC分类号： G01N21/49
摘要： Light having a predetermined wavelength and various incident angle components to be incident on a surface of a scattering medium to generate an equivalent point light source or a group of equivalent point light sources near or on the surface of the scattering medium, and light diffused during propagation in the scattering medium is detected outside, and considering isotropic light from the equivalent light sources is immediately diffused, the optically detected signal is processed to detect a predetermined parameter which is primary information and this predetermined parameter is processed to measure internal information which is secondary information in the scattering medium with high accuracy.
摘要翻译：具有预定波长的光和入射到散射介质的表面上的各种入射角分量的光，以在散射介质的表面附近或表面上产生等效点光源或一组等效点光源，以及在传播期间漫射的光在散射介质外部检测到，考虑到来自等效光源的各向同性光立即扩散，对光检测信号进行处理，以检测作为主要信息的预定参数，并且处理该预定参数以测量作为次要信息的内部信息在散射介质中具有高精度。

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