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1. 发明授权

US09932598B2 Metabolic engineering of microbial organisms 有权
公开(公告)号：US09932598B2
公开(公告)日：2018-04-03
申请号：US13957340
申请日：2013-08-01
申请人： The Regents of the University of California
发明人： Bernhard O. Palsson , Adam M. Feist
IPC分类号： C12N15/70 , C12P7/56 , C12N9/02 , C12N9/10
CPC分类号： C12N15/70 , C12N9/0008 , C12N9/1029 , C12P7/56 , C12Y102/04001 , C12Y203/01054
摘要： Microbial strains with desirable carbohydrate productions characteristics and methods of making and using the same are provided herein.

2. 发明申请

US20120156735A1 RECOMBINANT HOST CELLS COMPRISING PHOSPHOKETOLASES 有权
标题翻译：包含磷酸氢钠的重组宿主细胞
公开(公告)号：US20120156735A1
公开(公告)日：2012-06-21
申请号：US13161168
申请日：2011-06-15
申请人： Michael Dauner , Lori Ann Maggio-Hall , Jean-Francois Tomb
发明人： Michael Dauner , Lori Ann Maggio-Hall , Jean-Francois Tomb
IPC分类号： C12P7/16 , C12N1/21 , C12P7/18 , C12N15/63 , C12P13/08 , C12P13/06 , C12P7/56 , C12P7/46 , C12N1/19 , C12P7/26
CPC分类号： C12P7/16 , C12N9/0004 , C12N9/0008 , C12N9/1029 , C12N9/88 , C12N15/52 , C12N15/74 , C12N15/81 , C12P7/18 , C12P7/26 , C12P7/46 , C12P7/56 , C12P13/06 , C12P13/08 , C12Y102/01003 , C12Y102/07001 , C12Y114/15003 , C12Y118/01001 , C12Y118/01002 , C12Y203/01008 , C12Y203/01054 , C12Y401/01001 , C12Y401/02009 , Y02E50/10 , Y02P20/52
摘要： The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.
摘要翻译：本发明涉及重组宿主细胞，包括：（i）编码将丙酮酸转化为乙醛，乙酰磷酸或乙酰辅酶A的多肽的内源基因中的至少一个缺失，突变和/或取代; 和（ii）编码具有磷酸酮醇酶活性的多肽的异源多核苷酸。本发明还涉及重组宿主细胞，其还包含（iii）编码具有磷酸转乙酰酶活性的多肽的异源多核苷酸。

3. 发明申请

US20090203095A1 NOVEL ENGINEERED MICROORGANISM PRODUCING HOMO-SUCCINIC ACID AND METHOD FOR PREPARING SUCCINIC ACID USING THE SAME 有权
标题翻译：生产同型酸的新型工程化微生物及其制备方法
公开(公告)号：US20090203095A1
公开(公告)日：2009-08-13
申请号：US12359322
申请日：2009-01-25
申请人： Sang Yup LEE , Sung Won LIM , Hyohak SONG
发明人： Sang Yup LEE , Sung Won LIM , Hyohak SONG
IPC分类号： C12P7/46 , C12N1/21 , C12N15/87
CPC分类号： C12P7/46 , C12N1/20 , C12N9/0006 , C12N9/1029 , C12N9/1217 , C12N15/902 , C12Y203/01054 , Y02P20/52
摘要： The present invention relates to a mutant microorganism, which is selected from the group consisting of genus Mannheimia, genus Actinobacillus and genus Anaerobiospirillum, producing homo-succinic acid and a method for producing homo-succinic acid using the same, and more particularly to a mutant microorganism producing succinic acid at a high concentration while producing little or no other organic acids in anaerobic conditions, which is obtained by disrupting a gene encoding lactate dehydrogenase (ldhA), a gene encoding phosphotransacetylase (pta), and a gene encoding acetate kinase (ackA), without disrupting a gene encoding pyruvate formate lyase (pfl), as well as a method for producing succinic acid using the same. The inventive mutant microorganism has the property of having a high growth rate and succinic acid productivity while producing little or no organic acids, as compared to the prior strains producing succinic acid. Thus, the inventive mutant microorganism is useful to produce succinic acid for industrial use.
摘要翻译：本发明涉及一种突变体微生物，其选自人嗜血杆菌属（Mannheimia），放线杆菌属（Actinobacillus）和放线虫属（Anaerobiospirillum）属，产生同型琥珀酸，以及使用其制备均琥珀酸的方法，更具体地说，涉及突变体通过破坏编码乳酸脱氢酶（ldhA）的基因，编码磷酸转乙酰酶（pta）的基因和编码乙酸激酶（ackA）的基因而获得的在厌氧条件下产生很少或不存在其它有机酸的微生物，产生高浓度的琥珀酸），而不破坏编码丙酮酸甲酸裂合酶（pfl）的基因，以及使用其生产琥珀酸的方法。与生产琥珀酸的现有菌株相比，本发明的突变微生物具有生长速度快和琥珀酸生产率少的特性，同时产生很少或不含有机酸。因此，本发明的突变微生物可用于生产用于工业用途的琥珀酸。

4. 发明申请

US20070172937A1 Recombinant cells that highly express chromosomally-integrated heterologous genes 审中-公开
标题翻译：高度表达染色体整合的异源基因的重组细胞
公开(公告)号：US20070172937A1
公开(公告)日：2007-07-26
申请号：US11725728
申请日：2007-03-20
申请人： Lonnie Ingram , Kazuyoshi Ohta , Brent Wood
发明人： Lonnie Ingram , Kazuyoshi Ohta , Brent Wood
IPC分类号： C12N1/21 , C12N15/74
CPC分类号： C12N15/67 , C12N9/1029 , C12N15/74 , C12Y203/01054
摘要： Recombinant host cells are obtained that comprise (A) a heterologous, polypeptide-encoding polynucleotide segment, stably integrated into a chromosome, which is under transcriptional control of an endogenous promoter and (B) a mutation that effects increased expression of the heterologous segment, resulting in enhanced production by the host cells of each polypeptide encoded by that segment, relative to production of each polypeptide by the host cells in the absence of the mutation. The increased expression thus achieved is retained in the absence of conditions that select for cells displaying such increased expression. When the integrated segment comprises, for example, ethanol-production genes from an efficient ethanol producer like Zymomonas mobilis, recombinant Escherichia coli and other enteric bacterial cells within the present invention are capable of converting a wide range of biomass-derived sugars efficiently to ethanol.
摘要翻译：获得重组宿主细胞，其包含（A）编码染色体的异源，编码多肽的多核苷酸片段，其在内源性启动子的转录控制下（B）产生异源片段表达增加的突变，导致在不存在突变的情况下，通过宿主细胞增强由该片段编码的每种多肽的产生，相对于宿主细胞每种多肽的产生。在没有选择用于显示这种增加的表达的细胞的条件的情况下，保留了如此实现的增加的表达。当整合片段包括例如来自有效乙醇生产者的乙醇生产基因如运动发酵单胞菌，本发明中的重组大肠杆菌和其它肠道细菌细胞能够将大范围的生物质衍生的糖有效转化为乙醇。

5. 发明授权

US07192772B1 Recombinant cells that highly express chromosomally-integrated heterologous gene 失效
标题翻译：高度表达染色体整合的异源基因的重组细胞
公开(公告)号：US07192772B1
公开(公告)日：2007-03-20
申请号：US09620060
申请日：2000-07-21
申请人： Lonnie O. Ingram , Kazuyoshi Ohta , Brent E. Wood
发明人： Lonnie O. Ingram , Kazuyoshi Ohta , Brent E. Wood
IPC分类号： C12N15/00 , C12N1/20 , C07H21/04
CPC分类号： C12N15/67 , C12N9/1029 , C12N15/74 , C12Y203/01054
摘要： Recombinant host cells are obtained that comprise (A) a heterologous, polypeptide-encoding polynucleotide segment, stably integrated into a chromosome, which is under transcriptional control of an endogenous promoter and (B) a mutation that effects increased expression of the heterologous segment, resulting in enhanced production by the host cells of each polypeptide encoded by that segment, relative to production of each polypeptide by the host cells in the absence of the mutation. The increased expression thus achieved is retained in the absence of conditions that select for cells displaying such increased expression. When the integrated segment comprises, for example, ethanol-production genes from an efficient ethanol producer like Zymomonas mobilis, recombinant Escherichia coli and other enteric bacterial cells within the present invention are capable of converting a wide range of biomass-derived sugars efficiently to ethanol.
摘要翻译：获得重组宿主细胞，其包含（A）编码染色体的异源，编码多肽的多核苷酸片段，其在内源性启动子的转录控制下（B）产生异源片段表达增加的突变，导致在不存在突变的情况下，通过宿主细胞增强由该片段编码的每种多肽的产生，相对于宿主细胞每种多肽的产生。在没有选择用于显示这种增加的表达的细胞的条件的情况下，保留了如此实现的增加的表达。当整合片段包括例如来自有效乙醇生产者的乙醇生产基因如运动发酵单胞菌，本发明中的重组大肠杆菌和其它肠道细菌细胞能够将大范围的生物质衍生的糖有效转化为乙醇。

6. 发明申请

US20170088863A1 Recombinant Host Cells Comprising Phosphoketalase 审中-公开
公开(公告)号：US20170088863A1
公开(公告)日：2017-03-30
申请号：US15375858
申请日：2016-12-12
申请人： Butamax Advanced Biofuels LLC
发明人： Michael Dauner , Lori Ann Maggio-Hall , Jean-Francois Tomb
IPC分类号： C12P7/16 , C12N9/02 , C12N9/10 , C12N9/88
CPC分类号： C12P7/16 , C12N9/0004 , C12N9/0008 , C12N9/1029 , C12N9/88 , C12N15/52 , C12N15/74 , C12N15/81 , C12P7/18 , C12P7/26 , C12P7/46 , C12P7/56 , C12P13/06 , C12P13/08 , C12Y102/01003 , C12Y102/07001 , C12Y114/15003 , C12Y118/01001 , C12Y118/01002 , C12Y203/01008 , C12Y203/01054 , C12Y401/01001 , C12Y401/02009 , Y02E50/10 , Y02P20/52
摘要： The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.

7. 发明申请

US20150275242A1 MICROORGANISMS AND METHODS FOR PRODUCTION OF SPECIFIC LENGTH FATTY ALCOHOLS AND RELATED COMPOUNDS 审中-公开
标题翻译：用于生产特定长度脂肪醇及相关化合物的微生物和方法
公开(公告)号：US20150275242A1
公开(公告)日：2015-10-01
申请号：US14435723
申请日：2013-10-14
申请人： GENOMATICA, INC.
发明人： Robin E. Osterhout , Anthony P. Burgard
IPC分类号： C12P7/64 , C12N9/04 , C12N9/88 , C07C31/20 , C12N9/00 , C12N9/12 , C12N9/16 , C12N9/10 , C12N9/02
CPC分类号： C12P7/64 , C07C31/207 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/1025 , C12N9/1029 , C12N9/1217 , C12N9/1294 , C12N9/13 , C12N9/16 , C12N9/88 , C12N9/93 , C12N15/52 , C12P7/04 , C12P7/24 , C12P7/6409 , C12Y101/01035 , C12Y101/01037 , C12Y101/05004 , C12Y101/05006 , C12Y101/99033 , C12Y102/01 , C12Y102/01002 , C12Y102/01003 , C12Y102/0101 , C12Y102/01015 , C12Y102/01018 , C12Y102/01027 , C12Y102/01042 , C12Y102/0105 , C12Y102/01076 , C12Y102/05001 , C12Y103/01044 , C12Y103/99012 , C12Y203/01008 , C12Y203/01016 , C12Y203/01054 , C12Y203/01174 , C12Y203/03008 , C12Y207/02001 , C12Y207/02007 , C12Y207/09002 , C12Y208/03003 , C12Y301/03001 , C12Y301/03002 , C12Y301/0302 , C12Y301/0306 , C12Y401/01 , C12Y401/01001 , C12Y401/01003 , C12Y401/01007 , C12Y401/01009 , C12Y401/01031 , C12Y401/01032 , C12Y401/01049 , C12Y401/01074 , C12Y401/01082 , C12Y401/02005 , C12Y401/03006 , C12Y401/03034 , C12Y402/01017 , C12Y602/01001 , C12Y602/01013 , C12Y602/01018 , C12Y604/01001
摘要： The invention provides non-naturally occurring microbial organisms containing a fatty alcohol, fatty aldehyde or fatty acid pathway, wherein the microbial organisms selectively produce a fatty alcohol, fatty aldehyde or fatty acid of a specified length. Also provided are non-naturally occurring microbial organisms having a fatty alcohol, fatty aldehyde or fatty acid pathway, wherein the microbial organisms further include an acetyl-CoA pathway. In some aspects, the microbial organisms of the invention have select gene disruptions or enzyme attenuations that increase production of fatty alcohols, fatty aldehydes or fatty acids. The invention additionally provides methods of using the above microbial organisms to produce a fatty alcohol, a fatty aldehyde or a fatty acid.
摘要翻译：本发明提供了包含脂肪醇，脂肪醛或脂肪酸途径的非天然存在的微生物，其中微生物有选择地产生具有特定长度的脂肪醇，脂肪醛或脂肪酸。还提供了具有脂肪醇，脂肪醛或脂肪酸途径的非天然存在的微生物，其中所述微生物进一步包括乙酰辅酶A途径。在一些方面，本发明的微生物有选择性的基因破坏或酶减弱，其增加脂肪醇，脂肪醛或脂肪酸的产生。本发明另外提供使用上述微生物生产脂肪醇，脂肪醛或脂肪酸的方法。

8. 发明授权

US08883466B2 Bacterial cells exhibiting formate dehydrogenase activity for the manufacture of succinic acid 有权
标题翻译：表现出甲酸脱氢酶活性的细菌细胞用于制备琥珀酸
公开(公告)号：US08883466B2
公开(公告)日：2014-11-11
申请号：US12643058
申请日：2009-12-21
申请人： Edzard Scholten , Stefan Haefner , Hartwig Schröder
发明人： Edzard Scholten , Stefan Haefner , Hartwig Schröder
IPC分类号： C12P7/46 , C12N1/20 , C12P21/06 , C12P19/34 , C07H21/04 , C07K1/00 , C12N9/02 , C12N9/04 , C12N9/10
CPC分类号： C12N9/0006 , C12N9/0008 , C12N9/1029 , C12P7/46 , C12Y101/02003 , C12Y102/01002 , C12Y203/01054
摘要： The present invention relates to a bacterial cell of the genus Pasteurella comprising a heterologous polypeptide having formate dehydrogenase activity. The present invention also relates to a method of manufacturing succinic acid and the use of the bacterial cell for the manufacture of succinic acid.
摘要翻译：本发明涉及巴斯德氏菌属的细菌细胞，其包含具有甲酸脱氢酶活性的异源多肽。本发明还涉及制备琥珀酸的方法以及细菌细胞在制备琥珀酸中的用途。

9. 发明授权

US08673598B2 Microbial succinic acid producers and purification of succinic acid 有权
标题翻译：微生物琥珀酸生产商和琥珀酸的纯化
公开(公告)号：US08673598B2
公开(公告)日：2014-03-18
申请号：US13201547
申请日：2010-02-12
申请人： Hartwig Schroder , Stefan Haefner , Gregory Von Abendroth , Rajan Hollmann , Aline Raddatz , Hansgeorg Ernst , Hans Gurski
发明人： Hartwig Schroder , Stefan Haefner , Gregory Von Abendroth , Rajan Hollmann , Aline Raddatz , Hansgeorg Ernst , Hans Gurski
IPC分类号： C12N1/32 , C12P7/46 , C12P1/04 , C12P17/10 , C12P7/40
CPC分类号： C12P7/46 , C12N1/32 , C12N9/1029 , C12R1/01 , C12Y203/01054
摘要： The present invention relates to bacterial strains, capable of utilizing glycerol as a carbon source for the fermentative production of succinic acid, wherein said strains are genetically modified so that they comprise a deregulation of their endogenous pyruvate-formate-lyase enzyme activity, as well as to methods of producing organic acids, in particular succinic acid, by making use of such microorganism. The present invention also relates to the downstream processing of the produced organic acids by cation exchange chromatography.
摘要翻译：本发明涉及能够利用甘油作为发酵生产琥珀酸的碳源的细菌菌株，其中所述菌株被遗传修饰，使得它们包含其内源性丙酮酸 - 甲酸裂解酶活性的失调，以及涉及通过利用这种微生物生产有机酸，特别是琥珀酸的方法。本发明还涉及通过阳离子交换层析对所生产的有机酸的下游加工。

10. 发明申请

US20110300589A1 Novel Microbial Succinic Acid Producers and Purification of Succinic Acid 有权
标题翻译：新型微生物琥珀酸生产商和琥珀酸的纯化
公开(公告)号：US20110300589A1
公开(公告)日：2011-12-08
申请号：US13201547
申请日：2010-02-12
申请人： Hartwig Schroder , Stefan Haefner , Gregory Von Abendroth , Rajan Hollmann , Aline Raddatz , Hansgeorg Ernst , Hans Gurski
发明人： Hartwig Schroder , Stefan Haefner , Gregory Von Abendroth , Rajan Hollmann , Aline Raddatz , Hansgeorg Ernst , Hans Gurski
IPC分类号： C12P7/46 , C12P1/04 , C12P17/04 , C12P17/10 , C12N1/20 , C12P7/40
CPC分类号： C12P7/46 , C12N1/32 , C12N9/1029 , C12R1/01 , C12Y203/01054
摘要： The present invention relates to bacterial strains, capable of utilizing glycerol as a carbon source for the fermentative production of succinic acid, wherein said strains are genetically modified so that they comprise a deregulation of their endogenous pyruvate-formate-lyase enzyme activity, as well as to methods of producing organic acids, in particular succinic acid, by making use of such microorganism. The present invention also relates to the downstream processing of the produced organic acids by cation exchange chromatography.
摘要翻译：本发明涉及能够利用甘油作为发酵生产琥珀酸的碳源的细菌菌株，其中所述菌株被遗传修饰，使得它们包含其内源性丙酮酸 - 甲酸裂解酶活性的失调，以及涉及通过利用这种微生物生产有机酸，特别是琥珀酸的方法。本发明还涉及通过阳离子交换层析对所生产的有机酸的下游加工。

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