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1. 发明授权

US08652817B2 Recombinant host cells and media for ethanol production 失效
标题翻译：用于乙醇生产的重组宿主细胞和培养基
公开(公告)号：US08652817B2
公开(公告)日：2014-02-18
申请号：US11922631
申请日：2006-06-30
申请人： Brent E. Wood , Lonnie O. Ingram , Lorraine P. Yomano , Sean W. York
发明人： Brent E. Wood , Lonnie O. Ingram , Lorraine P. Yomano , Sean W. York
IPC分类号： C12P7/06
CPC分类号： C12N1/22 , C12P7/065 , Y02E50/17
摘要： Disclosed are recombinant host cells suitable for degrading an oligosaccharide that have been optimized for growth and production of high yields of ethanol, and methods of making and using these cells. The invention further provides minimal media comprising urea-like compounds for economical production of ethanol by recombinant microorganisms. Recombinant host cells in accordance with the invention are modified by gene mutation to eliminate genes responsible for the production of unwanted products other than ethanol, thereby increasing the yield of ethanol produced from the oligosaccharides, relative to unmutated parent strains. The new and improved strains of recombinant bacteria are capable of superior ethanol productivity and yield when grown under conditions suitable for fermentation in minimal growth media containing inexpensive reagents. Systems optimized for ethanol production combine a selected optimized minimal medium with a recombinant host cell optimized for use in the selected medium. Preferred systems are suitable for efficient ethanol production by simultaneous saccharification and fermentation (SSF) using lignocellulose as an oligosaccharide source. The invention also provides novel isolated polynucleotide sequences, polypeptide sequences, vectors and antibodies.
摘要翻译：公开了适用于降解生物和生产高产率乙醇的低聚糖的重组宿主细胞，以及制备和使用这些细胞的方法。本发明还提供了包含尿素样化合物的基本培养基，用于通过重组微生物经济地生产乙醇。根据本发明的重组宿主细胞通过基因突变修饰以消除负责生产除乙醇以外的不需要的产物的基因，从而相对于未突变的亲本菌株增加了由寡糖产生的乙醇的产率。当在含有廉价试剂的最小生长培养基中适于发酵的条件下生长时，新的和改良的重组细菌菌株能够获得优异的乙醇生产力和产量。优化用于乙醇生产的系统将选定的优化的基本培养基与优化用于所选培养基的重组宿主细胞结合。优选的系统适用于通过使用木素纤维素作为寡糖来源的同时糖化和发酵（SSF）来有效地生产乙醇。本发明还提供了新的分离的多核苷酸序列，多肽序列，载体和抗体。

2. 发明授权

US08465953B2 Ethanol production in non-recombinant hosts 有权
标题翻译：非重组宿主中的乙醇生产
公开(公告)号：US08465953B2
公开(公告)日：2013-06-18
申请号：US12298216
申请日：2007-04-26
申请人： Youngnyun Kim , Keelnatham Shanmugam , Lonnie O. Ingram
发明人： Youngnyun Kim , Keelnatham Shanmugam , Lonnie O. Ingram
IPC分类号： C12P7/06 , C12N9/00 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12N15/01 , C12N9/0051 , C12P7/06 , Y02E50/17
摘要： Non-recombinant bacteria that produce ethanol as the primary fermentation product, associated nucleic acids and polypeptides, methods for producing ethanol using the bacteria, and kits are disclosed.
摘要翻译：公开了产生乙醇作为主要发酵产物，相关核酸和多肽的非重组细菌，使用细菌生产乙醇的方法和试剂盒。

3. 发明申请

US20130098356A1 METHODS OF USING CELLULASE FOR REDUCING THE VISCOSITY OF FEEDSTOCK 有权
标题翻译：使用纤维素酶降低饲料粘度的方法
公开(公告)号：US20130098356A1
公开(公告)日：2013-04-25
申请号：US13583132
申请日：2011-03-10
申请人： Lonnie O. Ingram , Claudia C. Geddes , James J. Peterson , Michael T. Mullinnix , Keelnatham Shanmugam
发明人： Lonnie O. Ingram , Claudia C. Geddes , James J. Peterson , Michael T. Mullinnix , Keelnatham Shanmugam
IPC分类号： C12P19/14
CPC分类号： C12P19/14 , C12P19/02 , C12P2203/00
摘要： The invention provides methods for treatment of feedstock to reduce the relative viscosity and promote release of fermentable sugars.
摘要翻译：本发明提供了用于处理原料以降低相对粘度并促进可发酵糖释放的方法。

4. 发明申请

US20120108855A1 INCREASED EXPRESSION OF TRANSHYDROGENASE GENES AND THEIR USE IN ETHANOL PRODUCTION 审中-公开
标题翻译：转基因基因的增加表达及其在乙醇生产中的应用
公开(公告)号：US20120108855A1
公开(公告)日：2012-05-03
申请号：US13320633
申请日：2010-03-15
申请人： Lonnie O. Ingram , Elliot N. Miller , Laura R. Jarboe
发明人： Lonnie O. Ingram , Elliot N. Miller , Laura R. Jarboe
IPC分类号： C07C31/08 , C12P7/06 , C12N1/21
CPC分类号： C12N1/22 , C12P7/10 , Y02E50/16 , Y02E50/17
摘要： The invention provides isolated or recombinant ethanologenic bacteria that have increased expression of transhydrogenase genes and methods of preparation. The invention also provides methods of producing ethanol using the bacterium and corresponding kits.
摘要翻译：本发明提供了具有增加的转氢酶基因表达和制备方法的分离或重组的产乙醇细菌。本发明还提供使用细菌和相应试剂盒生产乙醇的方法。

5. 发明授权

US08900835B2 Engineering of thermotolerant Bacillus coagulans for production of D(−)-lactic acid 有权
标题翻译：用于生产D（ - ） - 乳酸的耐热凝固芽孢杆菌的工程
公开(公告)号：US08900835B2
公开(公告)日：2014-12-02
申请号：US13301836
申请日：2011-11-22
申请人： Qingzhao Wang , Keelnatham T. Shanmugam , Lonnie O. Ingram
发明人： Qingzhao Wang , Keelnatham T. Shanmugam , Lonnie O. Ingram
IPC分类号： C12P7/56 , C12N1/20 , C12N9/04 , C12N9/10
CPC分类号： C12P7/56 , C12N9/0006 , C12N9/1022
摘要： Genetically modified microorganisms having the ability to produce D(−)-lactic acid at temperatures between 30° C. and 55° C. are provided. In various embodiments, the microorganisms may have the chromosomal lactate dehydrogenase (ldh) gene and/or the chromosomal acetolactate synthase (alsS) gene inactivated. Exemplary microorganisms for use in the disclosed methods are Bacillus spp., such as Bacillus coagulans.
摘要翻译：提供了在30℃和55℃之间的温度下生产D（ - ） - 乳酸的能力的转基因微生物。在各种实施方案中，微生物可以具有染色体乳酸脱氢酶（ldh）基因和/或染色体乙酰乳酸合酶（alsS）基因失活。用于所公开方法的示例性微生物是芽孢杆菌属如凝结芽孢杆菌。

6. 发明申请

US20100196978A1 Recombinant Host Cells and Media for Ethanol Production 失效
标题翻译：重组宿主细胞和乙醇生产的培养基
公开(公告)号：US20100196978A1
公开(公告)日：2010-08-05
申请号：US11922631
申请日：2006-06-30
申请人： Brent E. Wood , Lonnie O. Ingram , Lorraine P. Yomano , Sean W. York
发明人： Brent E. Wood , Lonnie O. Ingram , Lorraine P. Yomano , Sean W. York
IPC分类号： C12P7/06 , C12N5/10 , C12N5/07 , C12N1/21 , C12N1/15 , C12N1/19 , C12N15/09 , C12N5/00 , C07H21/04 , C07K14/26
CPC分类号： C12N1/22 , C12P7/065 , Y02E50/17
摘要： Disclosed are recombinant host cells suitable for degrading an oligosaccharide that have been optimized for growth and production of high yields of ethanol, and methods of making and using these cells. The invention further provides minimal media comprising urea-like compounds for economical production of ethanol by recombinant microorganisms. Recombinant host cells in accordance with the invention are modified by gene mutation to eliminate genes responsible for the production of unwanted products other than ethanol, thereby increasing the yield of ethanol produced from the oligosaccharides, relative to unmutated parent strains. The new and improved strains of recombinant bacteria are capable of superior ethanol productivity and yield when grown under conditions suitable for fermentation in minimal growth media containing inexpensive reagents. Systems optimized for ethanol production combine a selected optimized minimal medium with a recombinant host cell optimized for use in the selected medium. Preferred systems are suitable for efficient ethanol production by simultaneous saccharification and fermentation (SSF) using lignocellulose as an oligosaccharide source. The invention also provides novel isolated polynucleotide sequences, polypeptide sequences, vectors and antibodies.
摘要翻译：公开了适用于降解生物和生产高产率乙醇的低聚糖的重组宿主细胞，以及制备和使用这些细胞的方法。本发明还提供了包含尿素样化合物的基本培养基，用于通过重组微生物经济地生产乙醇。根据本发明的重组宿主细胞通过基因突变修饰以消除负责生产除乙醇以外的不需要的产物的基因，从而相对于未突变的亲本菌株增加了由寡糖产生的乙醇的产率。当在含有廉价试剂的最小生长培养基中适于发酵的条件下生长时，新的和改良的重组细菌菌株能够获得优异的乙醇生产力和产量。优化用于乙醇生产的系统将选定的优化的基本培养基与优化用于所选培养基的重组宿主细胞结合。优选的系统适用于通过使用木素纤维素作为寡糖来源的同时糖化和发酵（SSF）来有效地生产乙醇。本发明还提供了新的分离的多核苷酸序列，多肽序列，载体和抗体。

7. 发明申请

US20090286293A1 ETHANOL PRODUCTION IN NON-RECOMBINANT HOSTS 有权
标题翻译：非重组生物中的乙醇生产
公开(公告)号：US20090286293A1
公开(公告)日：2009-11-19
申请号：US12298216
申请日：2007-04-26
申请人： Youngnyun Kim , Keelnatham Shanmugam , Lonnie O. Ingram
发明人： Youngnyun Kim , Keelnatham Shanmugam , Lonnie O. Ingram
IPC分类号： C12P7/06 , C12N1/20 , C07H21/00 , C07K14/00 , C07K7/08
CPC分类号： C12N15/01 , C12N9/0051 , C12P7/06 , Y02E50/17
摘要： Non-recombinant bacteria that produce ethanol as the primary fermentation product, associated nucleic acids and polypeptides, methods for producing ethanol using the bacteria, and kits are disclosed.
摘要翻译：公开了产生乙醇作为主要发酵产物，相关核酸和多肽的非重组细菌，使用细菌生产乙醇的方法和试剂盒。

8. 发明授权

US5424202A Ethanol production by recombinant hosts 失效
标题翻译：重组宿主生产乙醇
公开(公告)号：US5424202A
公开(公告)日：1995-06-13
申请号：US846344
申请日：1992-03-06
申请人： Lonnie O. Ingram , David S. Beall , Gerhard F. H. Burchhardt , Walter V. Guimaraes , Kazuyoshi Ohta , Brent E. Wood , Keelnatham T. Shanmugam
发明人： Lonnie O. Ingram , David S. Beall , Gerhard F. H. Burchhardt , Walter V. Guimaraes , Kazuyoshi Ohta , Brent E. Wood , Keelnatham T. Shanmugam
IPC分类号： C12M1/00 , C12M1/02 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/04 , C12N9/24 , C12N9/88 , C12N15/09 , C12N15/52 , C12P7/06 , C12R1/18 , G06T1/00 , C12N15/63 , C12P7/12
CPC分类号： C12N9/0006 , C12N15/52 , C12N9/248 , C12N9/88 , C12P7/065 , C12Y101/01001 , G06T1/00 , E05Y2900/132 , Y02E50/17 , Y10S435/847 , Y10S435/854
摘要： Novel plasmids comprising genes which code for the alcohol dehydrogenase and pyruvate decarboxylase are described. Also described are recombinant hosts which have been transformed with genes coding for alcohol dehydrogenase and pyruvate. By virtue of their transformation with these genes, the recombinant hosts are capable of producing significant amounts of ethanol as a fermentation product. Also disclosed are methods for increasing the growth of recombinant hosts and methods for reducing the accumulation of undesirable metabolic products in the growth medium of these hosts. Also disclosed are recombinant host capable of producing significant amounts of ethanol as a fermentation product of oligosaccharides and plasmids comprising genes encoding polysaccharases, in addition to the genes described above which code for the alcohol dehydrogenase and pyruvate decarboxylase. Further, methods are described for producing ethanol from oligomeric feedstock using the recombinant hosts described above. Also provided is a method for enhancing the production of functional proteins in a recombinant host comprising overexpressing an adhB gene in the host. Further provided are process designs for fermenting oligosaccharide-containing biomass to ethanol.
摘要翻译：描述了包含编码醇脱氢酶和丙酮酸脱羧酶的基因的新型质粒。还描述了已经用编码醇脱氢酶和丙酮酸的基因转化的重组宿主。由于它们与这些基因的转化，重组宿主能够产生显着量的乙醇作为发酵产物。还公开了增加重组宿主生长的方法和减少这些宿主生长培养基中不需要的代谢产物积累的方法。还公开了能够产生显着量的乙醇作为寡糖的发酵产物的重组宿主和包含编码多糖酶的基因的质粒，除了上述描述了醇脱氢酶和丙酮酸脱羧酶的基因之外。此外，描述了使用上述重组宿主从低聚原料生产乙醇的方法。还提供了用于增强重组宿主中功能蛋白质生产的方法，其包括在宿主中过表达adhB基因。进一步提供了将含有寡糖的生物质发酵成乙醇的方法设计。

9. 发明授权

US5162516A Cloning and sequencing of the alcohol dehydrogenase II gene from Zymomonas mobilis 失效
标题翻译：来自运动发酵单胞菌的醇脱氢酶II基因的克隆和测序
公开(公告)号：US5162516A
公开(公告)日：1992-11-10
申请号：US200110
申请日：1988-05-31
申请人： Lonnie O. Ingram , Tyrrell Conway
发明人： Lonnie O. Ingram , Tyrrell Conway
IPC分类号： C12N9/04 , C12N9/88 , C12N15/12 , C12N15/70
CPC分类号： C12N9/0006 , C12N15/70 , C12N9/88
摘要： The alcohol dehydrogenase II gene from Zymomonas mobilis has been cloned and sequenced. This gene can be expressed at high levels in other organisms to produce acetaldehyde or to convert acetaldehyde to ethanol.
摘要翻译：来自运动发酵单胞菌的醇脱氢酶II基因已被克隆并测序。该基因可以在其他生物体中以高水平表达以产生乙醛或将乙醛转化为乙醇。

10. 发明授权

US08716002B2 Re-engineering bacteria for ethanol production 失效
标题翻译：重新设计乙醇生产细菌
公开(公告)号：US08716002B2
公开(公告)日：2014-05-06
申请号：US12375484
申请日：2007-08-08
申请人： Lorraine P. Yomano , Sean W. York , Shengde Zhou , Keelnatham Shanmugam , Lonnie O. Ingram
发明人： Lorraine P. Yomano , Sean W. York , Shengde Zhou , Keelnatham Shanmugam , Lonnie O. Ingram
IPC分类号： C12N1/21 , C12N15/70
CPC分类号： C12P7/065 , Y02E50/17
摘要： The invention provides recombinant bacteria, which comprise a full complement of heterologous ethanol production genes. Expression of the full complement of heterologous ethanol production genes causes the recombinant bacteria to produce ethanol as the primary fermentation product when grown in mineral salts medium, without the addition of complex nutrients. Methods for producing the recombinant bacteria and methods for producing ethanol using the recombinant bacteria are also disclosed.
摘要翻译：本发明提供重组细菌，其包含全部异源乙醇生产基因。异源乙醇生产基因的完整补体的表达导致重组细菌在无矿物质培养基中生长时产生乙醇作为主要的发酵产物，而不添加复杂的营养物质。还公开了使用重组细菌生产重组细菌的方法和生产乙醇的方法。

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