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1. 发明申请

US20070172937A1 Recombinant cells that highly express chromosomally-integrated heterologous genes 审中-公开
标题翻译：高度表达染色体整合的异源基因的重组细胞
公开(公告)号：US20070172937A1
公开(公告)日：2007-07-26
申请号：US11725728
申请日：2007-03-20
申请人： Lonnie Ingram , Kazuyoshi Ohta , Brent Wood
发明人： Lonnie Ingram , Kazuyoshi Ohta , Brent Wood
IPC分类号： C12N1/21 , C12N15/74
CPC分类号： C12N15/67 , C12N9/1029 , C12N15/74 , C12Y203/01054
摘要： Recombinant host cells are obtained that comprise (A) a heterologous, polypeptide-encoding polynucleotide segment, stably integrated into a chromosome, which is under transcriptional control of an endogenous promoter and (B) a mutation that effects increased expression of the heterologous segment, resulting in enhanced production by the host cells of each polypeptide encoded by that segment, relative to production of each polypeptide by the host cells in the absence of the mutation. The increased expression thus achieved is retained in the absence of conditions that select for cells displaying such increased expression. When the integrated segment comprises, for example, ethanol-production genes from an efficient ethanol producer like Zymomonas mobilis, recombinant Escherichia coli and other enteric bacterial cells within the present invention are capable of converting a wide range of biomass-derived sugars efficiently to ethanol.
摘要翻译：获得重组宿主细胞，其包含（A）编码染色体的异源，编码多肽的多核苷酸片段，其在内源性启动子的转录控制下（B）产生异源片段表达增加的突变，导致在不存在突变的情况下，通过宿主细胞增强由该片段编码的每种多肽的产生，相对于宿主细胞每种多肽的产生。在没有选择用于显示这种增加的表达的细胞的条件的情况下，保留了如此实现的增加的表达。当整合片段包括例如来自有效乙醇生产者的乙醇生产基因如运动发酵单胞菌，本发明中的重组大肠杆菌和其它肠道细菌细胞能够将大范围的生物质衍生的糖有效转化为乙醇。

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