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1. 发明申请

US20130244270A1 MICROFLUIDIC DEVICE FOR CELL MOTILITY SCREENING AND CHEMOTAXIS TESTING 审中-公开
标题翻译：用于细胞动力学筛选和化学检测的微流体装置
公开(公告)号：US20130244270A1
公开(公告)日：2013-09-19
申请号：US13814426
申请日：2011-08-10
申请人： Lan Xie , Rui Ma , Chao Han , Wanli Xing , Jing Cheng
发明人： Lan Xie , Rui Ma , Chao Han , Wanli Xing , Jing Cheng
IPC分类号： C12Q1/02
CPC分类号： C12Q1/025 , B01L3/5027 , B01L2300/0816 , B01L2300/0864 , G01N33/5029
摘要： The present invention relates to a microfluidic device used for cell motility screening and chemotaxis testing which comprises microfluidic channels and chambers. Cells which can secret a chemoattractant or chemorepellent are selectively planted in the microfluidic device and a stable chemoattractant or chemorepellent gradient can be established in the channels.
摘要翻译：本发明涉及用于细胞运动筛选和趋化性测试的微流体装置，其包括微流体通道和腔室。可以将化学引诱物或化学引诱物分泌的细胞选择性地种植在微流体装置中，并且可以在通道中建立稳定的化学引诱物或化学增益梯度。

2. 发明授权

US09511366B2 Microfluidic device and its use for positioning of cells or organisms 有权
标题翻译：微流体装置及其用于定位细胞或生物体的用途
公开(公告)号：US09511366B2
公开(公告)日：2016-12-06
申请号：US13991848
申请日：2012-01-13
申请人： Rui Ma , Lan Xie , Tian Qiu , Lei Wang , Wanli Xing , Jing Cheng
发明人： Rui Ma , Lan Xie , Tian Qiu , Lei Wang , Wanli Xing , Jing Cheng
IPC分类号： B01L3/00 , C12M3/00 , C12M1/32 , C12M3/06
CPC分类号： B01L3/5027 , C12M21/06 , C12M23/12 , C12M23/16
摘要： A micro fluidic device comprises one microstructure layer (5) and one cover layer (1), wherein the cover layer (1) is connected to the microstructure layer (5). The microstructure layer (5) comprises one bottom layer and a plurality of microstructures on it to position samples. The cover layer (1) comprises one top layer, one positioning well (6) and at least one inlet pool (4). The positioning well (6) is right above the microstructures and connected with each other. The inlet pools (4) and the positioning well (6) are connected by microchannels (3) which are formed between the microstructure layer (5) and the cover layer (1). The micro fluidic device can be applied in vitro fertilization, in determining how glial cells affect neurons, in constructing neural network and in detecting cell growth conditions.
摘要翻译：微流体装置包括一个微结构层（5）和一个覆盖层（1），其中覆盖层（1）连接到微结构层（5）。微结构层（5）包括一个底层和多个微结构，用于定位样品。覆盖层（1）包括一个顶层，一个定位孔（6）和至少一个入口池（4）。定位井（6）位于微结构的正上方并相互连接。入口池（4）和定位井（6）通过形成在微结构层（5）和覆盖层（1）之间的微通道（3）连接。微流体装置可以应用于体外受精，确定神经胶质细胞如何影响神经元，构建神经网络和检测细胞生长条件。

3. 发明授权

US10274479B2 Method for sperm motility evaluation and screening and its microfluidic device 有权
公开(公告)号：US10274479B2
公开(公告)日：2019-04-30
申请号：US14117597
申请日：2012-06-01
申请人： Tian Qiu , Rui Ma , Chao Han , Lan Xie , Jing Cheng
发明人： Tian Qiu , Rui Ma , Chao Han , Lan Xie , Jing Cheng
IPC分类号： B01L3/00 , A61B17/43 , G01N33/50
摘要： The present invention provides a microfluidic device and its use for cell motility classification. The microfluidic device comprises a fluid inlet, a sample inlet and a channel connecting the fluid inlet and the sample inlet, wherein the channel comprises at least two sections of different sizes, and the channel allows fluid flow from the fluid inlet to the sample inlet.

4. 发明申请

US20140199720A1 METHOD FOR SPERM MOTILITY EVALUATION AND SCREENING AND ITS MICROFLUIDIC DEVICE 审中-公开
标题翻译：用于动力学评估和筛选的方法及其微流体装置
公开(公告)号：US20140199720A1
公开(公告)日：2014-07-17
申请号：US14117597
申请日：2012-06-01
申请人： Tian Qiu , Rui Ma , Chao Han , Lan Xie , Jing Cheng
发明人： Tian Qiu , Rui Ma , Chao Han , Lan Xie , Jing Cheng
IPC分类号： G01N33/50 , A61B17/43
CPC分类号： G01N33/5005 , A61B17/43 , B01L3/502746 , B01L3/502761 , B01L2200/0652 , B01L2300/0825
摘要： The present invention provides a microfluidic device and its use for cell motility classification. The microfluidic device comprises a fluid inlet, a sample inlet and a channel connecting the fluid inlet and the sample inlet, wherein the channel comprises at least two sections of different sizes, and the channel allows fluid flow from the fluid inlet to the sample inlet.
摘要翻译：本发明提供一种微流体装置及其在细胞运动性分类中的应用。微流体装置包括流体入口，样品入口和连接流体入口和样品入口的通道，其中通道包括至少两个不同尺寸的区段，并且通道允许流体从流体入口流到样品入口。

5. 发明授权

US08784633B2 Automatic positioning and sensing microelectrode arrays 有权
标题翻译：自动定位和感应微电极阵列
公开(公告)号：US08784633B2
公开(公告)日：2014-07-22
申请号：US12667507
申请日：2007-07-04
申请人： Guangxin Xiang , Liangbin Pan , Wanli Xing , Lihua Huang , Zhongyao Yu , Jing Zhu , Yuxiang Zhou , Jing Cheng
发明人： Guangxin Xiang , Liangbin Pan , Wanli Xing , Lihua Huang , Zhongyao Yu , Jing Zhu , Yuxiang Zhou , Jing Cheng
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： G01N33/4836 , B01J2219/00653 , B01J2219/00743 , B01L3/502707 , B01L2300/0645 , B01L2400/0421 , B01L2400/0424
摘要： A microelectrode sensing device includes a substrate and an array of microelectrode sensors. Each sensor includes a first conductive layer that at least partially conducts electricity. The first conductive layer is formed above the substrate and patterned to include a recording electrode that measures electrical activities of target cells. Each sensor also includes a second conductive layer that at least partially conducts electricity. The second conductive layer is elevated above the first layer and patterned to include multiple positioning electrodes arranged to define a sensing region above the recording electrode. The positioning electrodes are designed to generate an electric field pattern in the sensing region to move and confine the target cells to a sub-region of the sensing region that at least partially overlaps the recording electrode.
摘要翻译：微电极感测装置包括基板和微电极传感器阵列。每个传感器包括至少部分地导电的第一导电层。第一导电层形成在衬底上并被图案化以包括测量靶细胞的电活动的记录电极。每个传感器还包括至少部分地传导电的第二导电层。第二导电层在第一层之上被升高并且被图案化以包括布置成限定记录电极上方的感测区域的多个定位电极。定位电极被设计成在感测区域中产生电场图案以移动并将目标单元限制在至少部分地与记录电极重叠的感测区域的子区域上。

6. 发明授权

US07632674B2 Apparatus for stimulating an animal cell and recording its physiological signal and production and use methods thereof 有权
标题翻译：用于刺激动物细胞并记录其生理信号的装置及其生产和使用方法
公开(公告)号：US07632674B2
公开(公告)日：2009-12-15
申请号：US10499098
申请日：2002-11-29
申请人： Wanli Xing , Zhe Yu , Guangxin Xiang , Liangbin Pan , Jing Cheng
发明人： Wanli Xing , Zhe Yu , Guangxin Xiang , Liangbin Pan , Jing Cheng
IPC分类号： C12M3/00
CPC分类号： G01N33/5438 , C12M35/02 , G01N2333/46 , G01N2500/10
摘要： The present invention provides an apparatus for stimulating an animal cell and recording its physiological signal and methods of making and using thereof. The purpose of the present invention is to provide an apparatus for stimulating an animal cell and recording its physiological signal that is efficient, convenient, and accurate. The apparatus for stimulating an animal cell and recording its physiological signal of the present invention comprises a poor conductive substrate, wherein on at least one surface of the substrate is provided at least one unit conductive polymer layer and at least one good conductive microelectrode.
摘要翻译：本发明提供一种用于刺激动物细胞并记录其生理信号的装置及其制造和使用方法。本发明的目的是提供一种用于刺激动物细胞并记录其生理信号的装置，其有效，方便和准确。用于刺激动物细胞并记录其本发明的生理信号的装置包括差的导电基底，其中在基底的至少一个表面上提供至少一个单位导电聚合物层和至少一个良好的导电微电极。

7. 发明申请

US20090143245A1 MICROARRAYS FOR GENOTYPING AND METHODS OF USE 审中-公开
标题翻译：用于基因组的微阵列和使用方法
公开(公告)号：US20090143245A1
公开(公告)日：2009-06-04
申请号：US12093504
申请日：2006-02-20
申请人： Huafang Gao , Ze Li , Dong Wang , Yanhua Liu , Xiang Liu , Yangzhou Jiang , Chuanzan Zhao , Li Li , Gengxin Lan , Tao Guo , Bin Cai , Wanli Xing , Yuxiang Zhou , Jing Cheng
发明人： Huafang Gao , Ze Li , Dong Wang , Yanhua Liu , Xiang Liu , Yangzhou Jiang , Chuanzan Zhao , Li Li , Gengxin Lan , Tao Guo , Bin Cai , Wanli Xing , Yuxiang Zhou , Jing Cheng
IPC分类号： C40B30/10 , C40B40/08 , C40B50/06
CPC分类号： C12Q1/6881 , C12Q1/6837 , C12Q2600/156
摘要： The present invention provides a microarray for detecting a genotype at a polymorphic site in a plurality of nucleic acid samples, comprising a first set of nucleic acid fragments derived from the samples and a second set of nucleic acid fragments derived from a plurality of references immobilized thereon. The invention also provides a microarray comprising a set of nucleic acid fragments immobilized on the surface of the microarray, wherein the nucleic acid fragments are derived from the samples by amplifying a region in the sample containing the polymorphism through asymmetric PCR amplification. Methods of using and making the microarrays are also provided.
摘要翻译：本发明提供了用于检测多个核酸样品中多态性位点处的基因型的微阵列，其包含衍生自样品的第一组核酸片段和衍生自多个固定在其上的参考文献的第二组核酸片段。本发明还提供了一种微阵列，其包含固定在微阵列表面上的一组核酸片段，其中通过不对称PCR扩增扩增含有多态性的样品中的区域，来从样品衍生出核酸片段。还提供了使用和制造微阵列的方法。

8. 发明申请

US20080124251A1 Sample Preparation Device for Extracting Drug Residue 有权
标题翻译：用于提取药物残留物的样品制备装置
公开(公告)号：US20080124251A1
公开(公告)日：2008-05-29
申请号：US11661019
申请日：2004-11-22
申请人： Renyuan Chen , Guoqing Wang , Feijun Xian , Wanli Xing , Xianhua Wang , Jing Cheng
发明人： Renyuan Chen , Guoqing Wang , Feijun Xian , Wanli Xing , Xianhua Wang , Jing Cheng
IPC分类号： B01L9/06 , B01L11/00 , B01L7/02
CPC分类号： G01N1/40 , G01N2001/2866
摘要： The present invention provides a sample preparation device for extracting drug residue. It comprises a case, in which a water bath, a vortex mixer, and a homogenizer are integrated. The case also has on it an integrated control panel for controlling heating temperature of the water bath and homogenization time of the homogenizer.
摘要翻译：本发明提供了用于提取药物残留物的样品制备装置。它包括其中集成了水浴，涡流混合器和均化器的壳体。该情况还具有用于控制水浴的加热温度和均化器的均化时间的集成控制面板。

9. 发明申请

US20060124152A1 Method and apparatus for washing solid substrate with ultrasonic wave after hybridization reaction 审中-公开
标题翻译：杂交反应后用超声波洗涤固体底物的方法和装置
公开(公告)号：US20060124152A1
公开(公告)日：2006-06-15
申请号：US10524903
申请日：2002-12-10
申请人： Gang Li , Wanli Xing , Ruige Wu , Min Guo , Jing Cheng
发明人： Gang Li , Wanli Xing , Ruige Wu , Min Guo , Jing Cheng
IPC分类号： B08B3/10
CPC分类号： B08B3/12 , C12Q1/6813 , C12Q2523/301
摘要： A method and apparatus for washing solid substrate with ultrasonic wave after hybridization reaction are provided. The method includes (1) putting the solid substrate into a container having a washing solution, on which the hybridization reaction has been completed, (2) using an ultrasonic generator to produce a certain strength of ultrasonic wave and transmit it into the washing solution, (3) washing the substrate by means of the cavitation effect being produced in the washing solution by the ultrasonic wave. Since the cavitation bubbles in the washing solution can produce intensive efflux and local microslipstream against the solid surface, evidently reducing the liquid surface tension and friction and enhance the liquid flow, non-specific adsorbate and deposit bound weakly on the solid substrate can be washed down rapidly.
摘要翻译：提供了一种用于在杂交反应之后用超声波洗涤固体底物的方法和装置。该方法包括：（1）将固体基质置于具有杂交反应完成的洗涤液的容器中，（2）使用超声波发生器产生一定强度的超声波并将其输送到洗涤液中，（3）通过超声波在洗涤液中产生的空化作用洗涤基板。由于洗涤液中的气穴气泡能够对固体表面产生强烈的流出和局部微滑流，明显降低了液面张力和摩擦力，增强了液体流动，固体基质上弱结合的非特异性吸附物和沉积物可以被冲洗掉迅速

10. 发明授权

US09310375B2 Luminophore-labeled molecules coupled with particles for microarray-based assays 有权
标题翻译：发光标记分子与颗粒偶联用于基于微阵列的测定
公开(公告)号：US09310375B2
公开(公告)日：2016-04-12
申请号：US13877320
申请日：2010-10-27
申请人： Huafang Gao , Guangxin Xiang , Di Jiang , Wanli Xing , Jing Cheng
发明人： Huafang Gao , Guangxin Xiang , Di Jiang , Wanli Xing , Jing Cheng
IPC分类号： G01N33/58 , C12Q1/68 , C40B30/04 , G01N33/543
CPC分类号： G01N33/582 , C12Q1/6816 , C12Q1/6837 , C12Q1/6883 , C12Q2600/156 , C12Q2600/158 , C12Q2600/16 , C40B30/04 , G01N33/54333 , G01N33/54386 , C12Q2563/103 , C12Q2565/501
摘要： A microarray-based assay is provided, which is used for analyzing molecular interactions, including polynucleotides, polypeptides, antibodies, small molecule compounds, peptides and carbohydrates. Such method comprises labeling a target molecule with a luminophore, coupling the target molecule to a particle, and binding to a probe molecule on microarray. In particular, multiplexed genetic analysis of nucleic acid fragments can be implemented. Specific genes, single nucleotide polymorphisms or gene mutations, such as deletions, insertions, and indels, can be identified. This technology, with high sensitivity, enables the detection and interpretation of molecular interactions in an efficient way.
摘要翻译：提供基于微阵列的测定法，其用于分析分子相互作用，包括多核苷酸，多肽，抗体，小分子化合物，肽和碳水化合物。这种方法包括用发光体标记目标分子，将靶分子偶联到颗粒上，并在微阵列上结合探针分子。特别地，可以实现核酸片段的多重遗传分析。可以鉴定特异性基因，单核苷酸多态性或基因突变，例如缺失，插入和插入。该技术具有高灵敏度，能够以有效的方式检测和解释分子相互作用。

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