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1. 发明授权

US06271444B1 Enhancer elements for increased translation in plant plastids 失效
标题翻译：用于增加植物质体翻译的增强因子
公开(公告)号：US06271444B1
公开(公告)日：2001-08-07
申请号：US09113690
申请日：1998-07-10
申请人： Kevin E. McBride , Jeffrey M. Staub
发明人： Kevin E. McBride , Jeffrey M. Staub
IPC分类号： C12N504
CPC分类号： C12N9/1092 , C07K14/61 , C07K14/8117 , C07K2319/00 , C12N15/8214 , C12N15/8257 , C12N15/8275
摘要： Provided are methods for increasing the production of protein in a plant cell by transforming plastids of plant cells with a construct comprising a promoter functional in a plant plastid, a ribosome binding site, DNA sequence of interest and a transcription termination region, and growing plant cells comprising the transformed plastids under conditions wherein the DNA encoding sequence is transcribed in the plastid. Also provided are methods for increasing protein production by fusing a coding sequence to a gene of interest to a secondary protein for cleavage or targetting of the protein of interest within the plastid, whereby high levels of expression of protein is achieved.
摘要翻译：提供了通过用植物质体，核糖体结合位点，感兴趣的DNA序列和转录终止区功能的启动子和转录终止区的构建体转化植物细胞的质体来增加植物细胞中蛋白质的产生的方法，以及生长的植物细胞在其中DNA编码序列在质体中转录的条件下包含转化的质体。还提供了通过将编码序列融合到目的基因的二级蛋白质用于在质体内切割或靶向目标蛋白质来增加蛋白质产生的方法，由此实现了高水平的蛋白质表达。

2. 发明授权

US06492578B1 Expression of herbicide tolerance genes in plant plastids 失效
标题翻译：除草剂耐受基因在植物质体中的表达
公开(公告)号：US06492578B1
公开(公告)日：2002-12-10
申请号：US09351123
申请日：1999-07-10
申请人： Jeffrey M. Staub , Peter Hajdukiewicz , Kevin E. McBride , Narender Nehra , David J. Schaaf , David M. Stalker , Guangning Ye
发明人： Jeffrey M. Staub , Peter Hajdukiewicz , Kevin E. McBride , Narender Nehra , David J. Schaaf , David M. Stalker , Guangning Ye
IPC分类号： C12N1582
CPC分类号： C12N9/1092 , C07K2319/00 , C12N9/14 , C12N9/88 , C12N15/8214 , C12N15/8274 , C12N15/8275 , C12N15/8278
摘要： Provided are constructs and methods for expressing herbicide tolerance genes in plastids of plant cells. Constructs include the components of a promoter functional in a plant plastid, a DNA sequence which is capable of conferring tolerance in a plant cell to at least one herbicide compound when said DNA sequence is transcribed in plastids of said plant cell and a transcription termination region. Herbicide tolerance is produced by transforming plastids with the constructs of the invention and growing plant cells comprising the transformed plastids under conditions wherein the DNA sequence is transcribed and plant plastids and cells containing the plastids are rendered tolerant to applications of at least one herbicide compound.
摘要翻译：提供了用于在植物细胞的质体中表达除草剂耐受基因的构建体和方法。构建体包括在植物质体中起作用的启动子的组分，当所述DNA序列在所述植物细胞的质体和转录终止区中转录时，能够赋予植物细胞对至少一种除草剂化合物的耐受性的DNA序列。通过用本发明的构建体转化质体和包含转化的质体的条件下生长的植物细胞产生除草剂耐受性，其中DNA序列被转录并且植物质体和包含质体的细胞对至少一种除草剂化合物的施用具有耐受性。

3. 发明授权

US06512162B2 Expression of eukaryotic peptides in plant plastids 失效
标题翻译：真核肽在植物质体中的表达
公开(公告)号：US06512162B2
公开(公告)日：2003-01-28
申请号：US09113244
申请日：1998-07-10
申请人： Kevin E. McBride , Narender Nehra , Douglas A. Russell , David M. Stalker , Jeffrey M. Staub
发明人： Kevin E. McBride , Narender Nehra , Douglas A. Russell , David M. Stalker , Jeffrey M. Staub
IPC分类号： C12N1582
CPC分类号： C12N15/8275 , C07K14/61 , C07K14/8117 , C07K2319/00 , C12N15/8214 , C12N15/8257
摘要： Constructs and methods are provided for expressing peptides derived from eukaryotic organisms in plant plastids. Constructs have a promoter functional in a plant plastid, a DNA sequence encoding a peptide derived from an eukaryotic organism and a transcription termination region. Other elements include a selectable marker for selection of plant cells comprising a plastid expressing the marker and DNA regions of homology to the genome of the plastid and optionally a ribosome binding site joined to the promoter. By methods using such constructs high levels of eukaryotic peptides, such as mammalian proteins, are produced in a plant cell by growing plant cells under conditions whereby the DNA encoding sequences re expressed to produce eukaryotic peptide in said plastid.
摘要翻译：提供构建体和方法用于表达在植物质体中衍生自真核生物的肽。构建体在植物质体中具有功能的启动子，编码来自真核生物的肽的DNA序列和转录终止区。其他元件包括用于选择植物细胞的选择性标记，其包括表达标记的质体和与质体的基因组同源的DNA区以及任选的与启动子连接的核糖体结合位点。通过使用这种构建的方法，通过在条件下生长植物细胞，在DNA质粒编码序列中再次表达以在所述质体中产生真核肽，从而在植物细胞中产生高水平的真核肽，例如哺乳动物蛋白质。

4. 发明授权

US07259293B2 Expression of eukaryotic peptides in plant plastids 有权
标题翻译：真核肽在植物质体中的表达
公开(公告)号：US07259293B2
公开(公告)日：2007-08-21
申请号：US10917774
申请日：2004-08-13
申请人： Jeffrey M. Staub
发明人： Jeffrey M. Staub
IPC分类号： C12N15/82 , C12N15/62 , C12N15/18 , C12N5/04 , C12N5/10 , A01H5/00 , A01H5/10
CPC分类号： C07K14/61 , C07K14/8117 , C07K2319/00 , C12N15/8214 , C12N15/8257 , C12N15/8275
摘要： Constructs and methods are provided for expressing peptides derived from eukaryotic organisms in plant plastids. Constructs have a promoter functional in a plant plastid, a DNA sequence encoding a peptide derived from an eukaryotic organism and a transcription termination region. Other elements include a selectable marker for selection of plant cells comprising a plastid expressing the marker and DNA regions of homology to the genome of the plastid and optionally a ribosome binding site joined to the promoter. By methods using such constructs high levels of eukaryotic peptides, such as mammalian proteins, are produced in a plant cell by growing plant cells under conditions whereby the DNA encoding sequences are expressed to produce eukaryotic peptide in said plastid.
摘要翻译：提供构建体和方法用于表达在植物质体中衍生自真核生物的肽。构建体在植物质体中具有功能的启动子，编码来自真核生物的肽的DNA序列和转录终止区。其他元件包括用于选择植物细胞的选择性标记，其包括表达标记的质体和与质体的基因组同源的DNA区以及任选的与启动子连接的核糖体结合位点。通过使用这种构建体的方法，通过在条件下培养植物细胞，在植物细胞中产生高水平的真核肽，例如哺乳动物蛋白质，由此DNA编码序列被表达以在所述质体中产生真核肽。

5. 发明授权

US06218145B1 Bacterial expression systems based on plastic or mitochondrial promoter combinations 失效
标题翻译：基于塑料或线粒体启动子组合的细菌表达系统
公开(公告)号：US06218145B1
公开(公告)日：2001-04-17
申请号：US09283419
申请日：1999-04-01
申请人： Gregg Bogosian , Julia P. O'Neil , Jeffrey M. Staub
发明人： Gregg Bogosian , Julia P. O'Neil , Jeffrey M. Staub
IPC分类号： C12P2102
CPC分类号： C12N9/1247 , C07K14/415 , C07K14/61 , C12N15/70
摘要： A composition for expression of a protein-encoding gene in a host cell is described, making use of an heterologous regulon. This composition provides a protein-encoding gene under control of a promoter heterologous to the host cell, and a gene for an RNA polymerase, preferably a single-subunit RNA polymerase that recognizes the promoter heterologous to the host cell. The gene for the RNA polymerase is under control of an inducible promoter recognized by the host cell. Also disclosed is a method for expressing the protein-encoding gene using the composition described.
摘要翻译：描述了在宿主细胞中表达蛋白质编码基因的组合物，利用异源调节子。该组合物提供在与宿主细胞异源的启动子控制下的蛋白质编码基因，以及RNA聚合酶的基因，优选识别与宿主细胞异源的启动子的单亚基RNA聚合酶。 RNA聚合酶的基因由宿主细胞识别的诱导型启动子控制。还公开了使用所述组合物表达蛋白质编码基因的方法。

6. 发明授权

US06849778B1 Methods and vectors for site-specific recombination in plant cell plastids 有权
公开(公告)号：US06849778B1
公开(公告)日：2005-02-01
申请号：US09688851
申请日：2000-10-16
申请人： Jeffrey M. Staub , Peter H. J. Hajdukiewicz , Larry Gilbertson
发明人： Jeffrey M. Staub , Peter H. J. Hajdukiewicz , Larry Gilbertson
IPC分类号： C12N15/82 , A01H1/00 , C07H21/04 , C12N15/74 , C12N15/87
CPC分类号： C12N15/8213 , C12N15/8214
摘要： Novel compositions and methods useful for genetic engineering of plant cells are provided. In particular, plastid constructs comprising recombining sites are provided. Such constructs find use in methods for site specific recombination in plant cell plastids. Plant cells and plants comprising the constructs described herein, as well as plant cells and plants produced by the methods of the present invention are of interest.

7. 发明授权

US09267144B2 Plastid transformation of maize 有权
标题翻译：玉米的质体转化
公开(公告)号：US09267144B2
公开(公告)日：2016-02-23
申请号：US12106073
申请日：2008-04-18
申请人： Vladimir Sidorov , Jeffrey M. Staub , Yuechun Wan , Guangning Ye
发明人： Vladimir Sidorov , Jeffrey M. Staub , Yuechun Wan , Guangning Ye
IPC分类号： C12N15/82
CPC分类号： C12N15/8214
摘要： A method is provided for transforming maize plants to express DNA sequences of interest from plant cell plastids. The method allows the transformation of maize plant tissue with heterologous DNA constructs by microprojectile bombardment of green light grown cultures and dark grown embryogenic cultures. The invention also provides for maize cells in which the plastids contain heterologous DNA constructs.
摘要翻译：提供了用于转化玉米植物以表达来自植物细胞质体的感兴趣的DNA序列的方法。该方法允许用异源DNA构建体通过微生物轰击绿光生长的培养物和黑暗生长的胚发生培养物转化玉米植物组织。本发明还提供其中质体含有异源DNA构建体的玉米细胞。

8. 发明授权

US08404927B2 Double-stranded RNA stabilized in planta 有权
标题翻译：双链RNA稳定在植物中
公开(公告)号：US08404927B2
公开(公告)日：2013-03-26
申请号：US11453155
申请日：2006-06-13
申请人： Edwards Allen , Larry A. Gilbertson , Elysia K. Krieger , James K. Roberts , Jeffrey M. Staub
发明人： Edwards Allen , Larry A. Gilbertson , Elysia K. Krieger , James K. Roberts , Jeffrey M. Staub
IPC分类号： A01H1/00 , C12N15/82 , C07H21/04
CPC分类号： C12N15/8216 , C12N15/8217 , C12N15/8218 , C12N15/8286 , Y02A40/162
摘要： This invention discloses recombinant DNA constructs that transcribe to RNA having improved resistance to a plant RNase III enzyme, and methods for their use in making transgenic plant cells, plants, seeds, and products derived therefrom. Also provided are compositions and methods for imparting to a plant resistance to a pest or pathogen of the plant.
摘要翻译：本发明公开了转录到对植物核糖核酸酶III酶具有改善的抗性的RNA的重组DNA构建体及其用于制备转基因植物细胞，植物，种子和由其衍生的产物的方法。还提供了赋予植物对植物的害虫或病原体的抗性的组合物和方法。

9. 发明申请

US20120023611A1 Isolated Novel Nucleic Acid and Protein Molecules from Corn and Methods of Using Thereof 审中-公开
公开(公告)号：US20120023611A1
公开(公告)日：2012-01-26
申请号：US13202619
申请日：2010-02-23
申请人： Yongwei Cao , Jeffrey M. Staub , Bo-Xing Qiu , James A. Morrell , Wei Wu
发明人： Yongwei Cao , Jeffrey M. Staub , Bo-Xing Qiu , James A. Morrell , Wei Wu
IPC分类号： A01H1/02 , C12N5/10 , C12N15/82 , C07H21/04 , C12N15/29 , A01H1/00 , A01H5/00 , A01H5/10 , C12N5/04 , C40B40/06
CPC分类号： C07K14/415 , C12N15/8247 , C12N15/8251 , C12N15/8261 , C12N15/8271 , C12N15/8273 , Y02A40/146
摘要： This disclosure provides purified nucleic acids and polypeptides and various uses of these sequences such as transcript profiling. Also provided are transgenic plants, seeds, and plant cells containing DNA for expression of the proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants, methods of making such plants and methods of making agricultural commodity including seeds and hybrid seeds from such plants.

10. 发明申请

US20080289063A1 Plastid Transformation of Maize 有权
标题翻译：玉米的质体转化
公开(公告)号：US20080289063A1
公开(公告)日：2008-11-20
申请号：US12106073
申请日：2008-04-18
申请人： Vladimir Sidorov , Jeffrey M. Staub , Yuechun Wan , Guangning Ye
发明人： Vladimir Sidorov , Jeffrey M. Staub , Yuechun Wan , Guangning Ye
IPC分类号： C12N15/82
CPC分类号： C12N15/8214
摘要： A method is provided for transforming maize plants to express DNA sequences of interest from plant cell plastids. The method allows the transformation of maize plant tissue with heterologous DNA constructs by microprojectile bombardment of green light grown cultures and dark grown embryogenic cultures. The invention also provides for maize cells in which the plastids contain heterologous DNA constructs.
摘要翻译：提供了用于转化玉米植物以表达来自植物细胞质体的感兴趣的DNA序列的方法。该方法允许用异源DNA构建体通过微生物轰击绿光生长的培养物和黑暗生长的胚发生培养物转化玉米植物组织。本发明还提供其中质体含有异源DNA构建体的玉米细胞。

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