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1. 发明公开

US20230313212A1 PLASTID TRANSFORMATION BY COMPLEMENTATION OF NUCLEAR MUTATIONS 审中-公开
公开(公告)号：US20230313212A1
公开(公告)日：2023-10-05
申请号：US18180488
申请日：2023-03-08
申请人： PLASTOMICS INC.
发明人： Jeffrey Staub
IPC分类号： C12N15/82
CPC分类号： C12N15/8214 , C12N15/8269
摘要： The disclosure provides a method of expressing an agronomically beneficial trait in a plant plastid comprising expressing an exogenous nucleic acid in the plant to produce non-photosynthetic mutant plants, and using callus grown from the mutant plants as recipients for introduction of a construct having a functional copy of the mutated gene and a gene conferring an agronomically beneficial trait. Embodiments provide for mutations in chloroplast-encoded genes, as well as mutations in nuclear-encoded genes targeted to the chloroplast that are required for photosynthesis. The disclosure also provides plants and plant parts produced from such methods, as well as kits for performing the methods as described.

2. 发明申请

US20190194265A1 COMPOSITIONS AND METHODS FOR SUPPRESSION OF INHIBITOR FORMATION AGAINST COAGULATION FACTORS IN HEMOPHILIA PATIENTS 审中-公开
公开(公告)号：US20190194265A1
公开(公告)日：2019-06-27
申请号：US16299928
申请日：2019-03-12
申请人： THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA , UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.
发明人： Roland W. Herzog , Henry Daniell
IPC分类号： C07K14/28 , A61K38/47 , C12N15/82 , C07K14/755 , A61K38/45 , A61K38/37 , A61K38/36
CPC分类号： C07K14/28 , A61K36/00 , A61K38/36 , A61K38/37 , A61K38/45 , A61K38/47 , C07K14/755 , C07K2319/00 , C12N15/8214 , C12N15/8257 , C12Y204/00 , C12Y302/0102 , C12Y302/01022 , Y02A50/471
摘要： Protein replacement therapy for patients with hemophilia or other inherited protein deficiencies is often complicated by pathogenic antibody responses, including antibodies that neutralize the therapeutic protein or that predispose to potentially life-threatening anaphylactic reactions by formation of IgE. Using murine and canine hemophilia as a model, we have developed a prophylactic protocol against such responses that is non-invasive and does not include immune suppression or genetic manipulation of the patient's cells. Oral delivery of a coagulation factor expressed in chloroplasts, bioencapsulated in plant cells, effectively blocked formation of inhibitory antibodies in protein replacement therapy. Inhibitor titers were mostly undetectable and up to 100-fold lower in treated subjects when compared to controls. Moreover, this treatment eliminated fatal anaphylactic reactions that occurred after four to six exposures to intravenous coagulation factor protein. Finally, the method can effectively be used to reverse or reduce undesirable pre-existing inhibitor titers.

3. 发明授权

US09724400B2 Administration of plant expressed oral tolerance agents 有权
公开(公告)号：US09724400B2
公开(公告)日：2017-08-08
申请号：US13508754
申请日：2010-11-09
申请人： Henry Daniell , Roland Herzog
发明人： Henry Daniell , Roland Herzog
IPC分类号： A61K38/36 , C12N9/64 , C12N15/82 , C12N15/15 , C07K14/745 , A61K39/00 , A61K38/48
CPC分类号： A61K39/0008 , A61K38/4846 , C07K2319/55 , C12N9/644 , C12N15/8214 , C12N15/8257 , C12Y304/21022
摘要： Protein replacement therapy for patients with hemophilia or other inherited protein deficiencies is often complicated by pathogenic antibody responses, including antibodies that neutralize the therapeutic protein or that predispose to potentially life-threatening anaphylactic reactions by formation of IgE. Using murine hemophilia B as a model, we have developed a prophylactic protocol against such responses that is non-invasive and does not include immune suppression or genetic manipulation of the patient's cells. Oral delivery of coagulation factor IX (F. IX) expressed in chloroplasts, bioencapsulated in plant cells, effectively blocked formation of inhibitory antibodies in protein replacement therapy. Inhibitor titers were mostly undetectable and up to 100-fold lower in treated mice when compared to controls. Moreover, this treatment eliminated fatal anaphylactic reactions that occurred after 4 to 6 exposures to intravenous F. IX protein. While only 20-25% of control animals survived after 6-8 F. IX doses, 90-95% of tolerized mice survived 12 injections without signs of allergy or anaphylaxis. This high-responder strain of hemophilia B mice represents the first hemophilic animal model to study anaphylactic reactions. The plant material was effective over a range of oral antigen doses (equivalent to 5-80 μg recombinant F.IX/kg), and controlled inhibitor formation and anaphylaxis long-term, up to 7 months. Oral antigen administration caused a deviant immune response that suppressed formation of IgE and inhibitory antibodies. This cost-effective and efficient approach to oral delivery of protein antigens to the gut should be applicable to several genetic diseases that are prone to pathogenic antibody responses during treatment.

4. 发明授权

US09657302B2 Expression of human interferon in transgenic chloroplasts 有权
公开(公告)号：US09657302B2
公开(公告)日：2017-05-23
申请号：US14537661
申请日：2014-11-10
申请人： The Trustees of the University of Pennsylvania
发明人： Henry Daniell
IPC分类号： C12N15/82 , C07K14/56
CPC分类号： C12N15/8214 , C07K14/56 , C12N15/8257
摘要： A plastid transformation vector for a stably transforming a plastid genome is provided. The vector includes, as operably-linked components, a first flanking sequence, a DNA sequence coding for a therapeutic human IFN, which is capable of expression in the plastid and a second flanking sequence. The invention also provides isolated and purified IFN, wherein the IFN is configured in a monomeric or multimeric form and is a structural equivalent to orally administered human IFN. Also provided are methods for variable-expressing biopharmaceutical proteins in plants suitable for mammal consumption. The method includes integrating a plastid transformation vector into a plastid genome of a plant cell; growing the plant cell to express a biopharmaceutical protein, such as therapeutic human interferon IFN. Also disclosed are plants transformed with the aforementioned vectors, and the progeny thereof. Also, disclosed is the IFN, which is IFNα2b.

5. 发明授权

US09506075B2 Bioluminescent plants comprising bacterial LUX operon and methods of making same 有权
标题翻译：包含细菌LUX操纵子的生物发光植物及其制备方法
公开(公告)号：US09506075B2
公开(公告)日：2016-11-29
申请号：US12670340
申请日：2008-07-31
申请人： Alexander Krichevsky
发明人： Alexander Krichevsky
IPC分类号： C12N15/82
CPC分类号： C12N15/8214 , C12N15/8212 , C12N15/8213 , C12N15/8242 , C12N15/825
摘要： In one aspect, the invention relates to a transgenic bioluminescent plant including an expressible heterologous nucleotide sequence comprising a bacterial LUX operon, which includes LUX A, LUX B, LUX C, LUX D, LUX E, and LUX G genes, wherein the heterologous nucleotide sequence is expressed to render the plant bioluminescent.
摘要翻译：一方面，本发明涉及一种转基因生物发光植物，其包含包含LUX A，LUX B，LUX C，LUX D，LUX E和LUX G基因的细菌LUX操纵子的可表达异源核苷酸序列，其中所述异源核苷酸表达序列以使植物生物发光。

6. 发明授权

US09493781B2 Synthetic brassica-derived chloroplast transit peptides 有权
标题翻译：合成芸苔属叶绿体转运肽
公开(公告)号：US09493781B2
公开(公告)日：2016-11-15
申请号：US13757613
申请日：2013-02-01
申请人： DOW AGROSCIENCES LLC
发明人： Justin M Lira , Robert Cicchillo , Carla N Yerkes , Andrew E Robinson
IPC分类号： C12N15/82 , C07K14/415 , C12N9/96 , C12N9/10
CPC分类号： C12N9/1092 , A01N57/20 , C07K14/415 , C07K2319/08 , C12N9/0069 , C12N9/0071 , C12N9/1085 , C12N9/96 , C12N15/62 , C12N15/8205 , C12N15/8209 , C12N15/8214 , C12N15/8221 , C12N15/8241 , C12N15/8245 , C12N15/8247 , C12N15/8274 , C12N15/8275 , C12N15/8281 , C12N15/8283 , C12N15/8286 , C12Y113/00 , C12Y114/11 , C12Y205/01019 , Y02A40/162
摘要： This disclosure concerns compositions and methods for targeting peptides, polypeptides, and proteins to plastids of plastid-containing cells. In some embodiments, the disclosure concerns chloroplast transit peptides that may direct a polypeptide to a plastid, and nucleic acid molecules encoding the same. In some embodiments, the disclosure concerns methods for producing a transgenic plant material (e.g., a transgenic plant) comprising a chloroplast transit peptide, as well as plant materials produced by such methods, and plant commodity products produced therefrom.
摘要翻译：本公开涉及将肽，多肽和蛋白质靶向含质体细胞的质体的组合物和方法。在一些实施方案中，本公开涉及可将多肽引导至质体的叶绿体转运肽以及编码其的核酸分子。在一些实施方案中，本公开涉及用于生产包含叶绿体转运肽的转基因植物材料（例如，转基因植物）的方法以及由这些方法生产的植物材料以及由其生产的植物商品。

7. 发明授权

US09447425B2 Transcription factor sequences for conferring advantageous properties to plants 有权
标题翻译：用于赋予植物有利性质的转录因子序列
公开(公告)号：US09447425B2
公开(公告)日：2016-09-20
申请号：US12573311
申请日：2009-10-05
申请人： Jacqueline E. Heard , Jose Luis Riechmann , Oliver Ratcliffe , Omaira Pineda , T. Lynne Reuber , James Zhang , Gregory Nadzan
发明人： Jacqueline E. Heard , Jose Luis Riechmann , Oliver Ratcliffe , Omaira Pineda , T. Lynne Reuber , James Zhang , Gregory Nadzan
IPC分类号： C12N15/82 , C07K14/415
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/8214 , C12N15/8241 , C12N15/8255 , C12N15/8267 , C12N15/827 , C12N15/8271 , C12N15/8273 , Y02A40/146
摘要： The invention relates to plant homeobox (HB) protein family polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties compared to a reference or control plant, including increased yield.
摘要翻译：本发明涉及植物同源异型盒（HB）蛋白质家族多肽，编码它们的多核苷酸，来自各种植物物种的同系物，以及使用多核苷酸和多肽产生与参考或对照植物相比具有优势性质的转基因植物的方法，包括增加产量。

8. 发明申请

US20160264983A1 METHODS FOR PLASTID TRANSFORMATION 审中-公开
标题翻译： PLASTID转换方法
公开(公告)号：US20160264983A1
公开(公告)日：2016-09-15
申请号：US15015968
申请日：2016-02-04
申请人： MONSANTO TECHNOLOGY LLC
发明人： BRIAN J. MARTINELL , Anna Mary O'Keefe , David Alan Somers , Edward James Williams , Xudong Ye
IPC分类号： C12N15/82
CPC分类号： C12N15/8214 , C12N15/8207 , C12N15/8213
摘要： Methods and compositions for plastid transformation and regeneration or development of transplastomic plants are provided. Embryo explants may be excised from seeds, and their meristematic tissue may be transformed directly without initiation of any callus phase before and/or after transformation. The present methods may be performed with fewer culturing steps relative to conventional methods, thereby enabling more rapid and efficient production of targeted transplastomic events in plants.
摘要翻译：提供了质体转化和转基因植物再生或发育的方法和组合物。胚外植体可以从种子中切除，并且它们的分生组织可以在转化前后和/或之后直接转化而不引发任何愈伤组织相。本方法可以相对于常规方法进行较少的培养步骤，从而使得能够更快速和有效地生产植物中靶向的转基因组学事件。

9. 发明授权

US09441238B2 Compositions and methods for modulating the sensitivity of cells to AHAS inhibitors 有权
标题翻译：用于调节细胞对AHAS抑制剂的敏感性的组合物和方法
公开(公告)号：US09441238B2
公开(公告)日：2016-09-13
申请号：US13871737
申请日：2013-04-26
申请人： SYNTHETIC GENOMICS, INC.
发明人： Michele M. Champagne , Jun Urano
IPC分类号： C12N15/82 , C12N9/10 , C12N15/79
CPC分类号： C12N15/8274 , C12N9/1022 , C12N15/79 , C12N15/8214 , C12N15/8278 , C12Y202/01006
摘要： Methods and materials useful for modulating the sensitivity of cells to an inhibitor of acetohydroxyacid synthase (AHAS) are disclosed. For example, nucleic acid molecules encoding AHAS large subunits are disclosed as well as methods for using such nucleic acid molecules to transform microbial cells and plant cells, and to confer modulated sensitivity to AHAS-inhibiting compounds onto such cells. Further provided are materials and methods useful for modulating growth, development, activity, and characteristics of host cells and organisms.
摘要翻译：公开了用于调节细胞对乙酰羟酸合成酶（AHAS）抑制剂的敏感性的方法和材料。例如，公开了编码AHAS大亚基的核酸分子以及使用这种核酸分子转化微生物细胞和植物细胞的方法，并赋予对这种细胞的AHAS抑制化合物的调节的敏感性。还提供了用于调节宿主细胞和生物体的生长，发育，活性和特征的材料和方法。

10. 发明授权

US09434952B2 Selectable marker gene and methods of use thereof in transplastomic plants 有权
标题翻译：可选标记基因及其在转基因植物中的应用方法
公开(公告)号：US09434952B2
公开(公告)日：2016-09-06
申请号：US13326295
申请日：2011-12-14
申请人： Pal Maliga
发明人： Pal Maliga
IPC分类号： C12N15/82
CPC分类号： C12N15/8214 , C12N15/8212
摘要： Compositions and method for the generation of transplastomic plants are provided.
摘要翻译：提供了用于产生转基因植物的组合物和方法。

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