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1. 发明授权

US07732662B2 Method for the transformation of plant cell plastids 有权
标题翻译：植物细胞质体转化方法
公开(公告)号：US07732662B2
公开(公告)日：2010-06-08
申请号：US11834512
申请日：2007-08-06
申请人： Jeffrey M Staub , Guangning Ye , Debra L. Broyles
发明人： Jeffrey M Staub , Guangning Ye , Debra L. Broyles
IPC分类号： C12N15/82
CPC分类号： C12N15/8214 , C12N15/8209 , C12N15/8275 , C12N15/8277
摘要： Novel compositions and methods useful for genetic engineering of plant cells to provide a method of producing plastid transformed plants are provided in the instant invention. In particular, the present invention provides methods for obtaining plastid transformed plants on medium containing plastid lethal compounds.
摘要翻译：在本发明中提供了用于植物细胞遗传工程以提供质量转化植物生产方法的新型组合物和方法。特别地，本发明提供了在含有质体致死化合物的培养基上获得质体转化植物的方法。

2. 发明授权

US07259293B2 Expression of eukaryotic peptides in plant plastids 有权
标题翻译：真核肽在植物质体中的表达
公开(公告)号：US07259293B2
公开(公告)日：2007-08-21
申请号：US10917774
申请日：2004-08-13
申请人： Jeffrey M. Staub
发明人： Jeffrey M. Staub
IPC分类号： C12N15/82 , C12N15/62 , C12N15/18 , C12N5/04 , C12N5/10 , A01H5/00 , A01H5/10
CPC分类号： C07K14/61 , C07K14/8117 , C07K2319/00 , C12N15/8214 , C12N15/8257 , C12N15/8275
摘要： Constructs and methods are provided for expressing peptides derived from eukaryotic organisms in plant plastids. Constructs have a promoter functional in a plant plastid, a DNA sequence encoding a peptide derived from an eukaryotic organism and a transcription termination region. Other elements include a selectable marker for selection of plant cells comprising a plastid expressing the marker and DNA regions of homology to the genome of the plastid and optionally a ribosome binding site joined to the promoter. By methods using such constructs high levels of eukaryotic peptides, such as mammalian proteins, are produced in a plant cell by growing plant cells under conditions whereby the DNA encoding sequences are expressed to produce eukaryotic peptide in said plastid.
摘要翻译：提供构建体和方法用于表达在植物质体中衍生自真核生物的肽。构建体在植物质体中具有功能的启动子，编码来自真核生物的肽的DNA序列和转录终止区。其他元件包括用于选择植物细胞的选择性标记，其包括表达标记的质体和与质体的基因组同源的DNA区以及任选的与启动子连接的核糖体结合位点。通过使用这种构建体的方法，通过在条件下培养植物细胞，在植物细胞中产生高水平的真核肽，例如哺乳动物蛋白质，由此DNA编码序列被表达以在所述质体中产生真核肽。

3. 发明授权

US06271444B1 Enhancer elements for increased translation in plant plastids 失效
标题翻译：用于增加植物质体翻译的增强因子
公开(公告)号：US06271444B1
公开(公告)日：2001-08-07
申请号：US09113690
申请日：1998-07-10
申请人： Kevin E. McBride , Jeffrey M. Staub
发明人： Kevin E. McBride , Jeffrey M. Staub
IPC分类号： C12N504
CPC分类号： C12N9/1092 , C07K14/61 , C07K14/8117 , C07K2319/00 , C12N15/8214 , C12N15/8257 , C12N15/8275
摘要： Provided are methods for increasing the production of protein in a plant cell by transforming plastids of plant cells with a construct comprising a promoter functional in a plant plastid, a ribosome binding site, DNA sequence of interest and a transcription termination region, and growing plant cells comprising the transformed plastids under conditions wherein the DNA encoding sequence is transcribed in the plastid. Also provided are methods for increasing protein production by fusing a coding sequence to a gene of interest to a secondary protein for cleavage or targetting of the protein of interest within the plastid, whereby high levels of expression of protein is achieved.
摘要翻译：提供了通过用植物质体，核糖体结合位点，感兴趣的DNA序列和转录终止区功能的启动子和转录终止区的构建体转化植物细胞的质体来增加植物细胞中蛋白质的产生的方法，以及生长的植物细胞在其中DNA编码序列在质体中转录的条件下包含转化的质体。还提供了通过将编码序列融合到目的基因的二级蛋白质用于在质体内切割或靶向目标蛋白质来增加蛋白质产生的方法，由此实现了高水平的蛋白质表达。

4. 发明授权

US06218145B1 Bacterial expression systems based on plastic or mitochondrial promoter combinations 失效
标题翻译：基于塑料或线粒体启动子组合的细菌表达系统
公开(公告)号：US06218145B1
公开(公告)日：2001-04-17
申请号：US09283419
申请日：1999-04-01
申请人： Gregg Bogosian , Julia P. O'Neil , Jeffrey M. Staub
发明人： Gregg Bogosian , Julia P. O'Neil , Jeffrey M. Staub
IPC分类号： C12P2102
CPC分类号： C12N9/1247 , C07K14/415 , C07K14/61 , C12N15/70
摘要： A composition for expression of a protein-encoding gene in a host cell is described, making use of an heterologous regulon. This composition provides a protein-encoding gene under control of a promoter heterologous to the host cell, and a gene for an RNA polymerase, preferably a single-subunit RNA polymerase that recognizes the promoter heterologous to the host cell. The gene for the RNA polymerase is under control of an inducible promoter recognized by the host cell. Also disclosed is a method for expressing the protein-encoding gene using the composition described.
摘要翻译：描述了在宿主细胞中表达蛋白质编码基因的组合物，利用异源调节子。该组合物提供在与宿主细胞异源的启动子控制下的蛋白质编码基因，以及RNA聚合酶的基因，优选识别与宿主细胞异源的启动子的单亚基RNA聚合酶。 RNA聚合酶的基因由宿主细胞识别的诱导型启动子控制。还公开了使用所述组合物表达蛋白质编码基因的方法。

5. 发明申请

US20120210458A9 Isolated Novel Nucleic Acid and Protein Molecules from Corn and Methods of Using Thereof 审中-公开
标题翻译：孤立的新型核酸和玉米蛋白质分子及其使用方法
公开(公告)号：US20120210458A9
公开(公告)日：2012-08-16
申请号：US13202619
申请日：2010-02-23
申请人： Yongwei Cao , Jeffrey M. Staub , Bo-Xing Qiu , James A. Morrell , Wei Wu
发明人： Yongwei Cao , Jeffrey M. Staub , Bo-Xing Qiu , James A. Morrell , Wei Wu
IPC分类号： A01H1/02 , C12N5/10 , C12N15/82 , C07H21/04 , C12N15/29 , A01H1/00 , A01H5/00 , A01H5/10 , C12N5/04 , C40B40/06
CPC分类号： C07K14/415 , C12N15/8247 , C12N15/8251 , C12N15/8261 , C12N15/8271 , C12N15/8273 , Y02A40/146
摘要： This disclosure provides purified nucleic acids and polypeptides and various uses of these sequences such as transcript profiling. Also provided are transgenic plants, seeds, and plant cells containing DNA for expression of the proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants, methods of making such plants and methods of making agricultural commodity including seeds and hybrid seeds from such plants.
摘要翻译：本公开提供了纯化的核酸和多肽以及这些序列的各种用途，例如转录物谱。还提供了转基因植物，种子和含有用于表达可用于赋予转基因作物植物增强的农学性状的蛋白质的DNA的植物细胞，以及制造这种植物的方法以及制造包括种子和杂交种子在内的农产品的方法从这样的植物。

6. 发明申请

US20120117685A1 Isolated Novel Nucleic Acid and Protein Molecules from Soybeans and Methods of Using Thos Molecules 审中-公开
标题翻译：孤立的新型核酸和蛋白质分子从大豆和使用Thos分子的方法
公开(公告)号：US20120117685A1
公开(公告)日：2012-05-10
申请号：US13202625
申请日：2010-02-23
申请人： Wei Wu , Jeffrey M. Staub , James A. Morrell , Bo-Xing Qiu , YongWei Cao
发明人： Wei Wu , Jeffrey M. Staub , James A. Morrell , Bo-Xing Qiu , YongWei Cao
IPC分类号： A01H5/10 , C12N15/82 , C40B40/06 , A01H1/02 , C12N15/29 , C12N5/10 , A01H5/00
CPC分类号： C07K14/415 , C12N15/8247 , C12N15/8251 , C12N15/8261 , C12N15/8271 , C12N15/8273 , Y02A40/146
摘要： This disclosure provides purified nucleic acids and polypeptides and various uses of these sequences such as transcript profiling. Also provided are transgenic plants, seeds, and plant cells containing DNA for expression of the proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants, methods of making such plants and methods of making agricultural commodity including seeds and hybrid seeds from such plants.
摘要翻译：本公开提供了纯化的核酸和多肽以及这些序列的各种用途，例如转录物谱。还提供了转基因植物，种子和含有用于表达可用于赋予转基因作物植物增强的农学性状的蛋白质的DNA的植物细胞，以及制造这种植物的方法以及制造包括种子和杂交种子在内的农产品的方法从这样的植物。

7. 发明授权

US06812379B2 Expression of eukaryotic peptides in plant plastids 失效
标题翻译：真核肽在植物质体中的表达
公开(公告)号：US06812379B2
公开(公告)日：2004-11-02
申请号：US10103516
申请日：2002-03-20
申请人： Jeffrey M. Staub
发明人： Jeffrey M. Staub
IPC分类号： C12N1582
CPC分类号： C07K14/61 , C07K14/8117 , C07K2319/00 , C12N15/8214 , C12N15/8257 , C12N15/8275
摘要： Constructs and methods are provided for expressing peptides derived from eukaryotic organisms in plant plastids. Constructs have a promoter functional in a plant plastid, a DNA sequence encoding a peptide derived from an eukaryotic organism and a transcription termination region. Other elements include a selectable marker for selection of plant cells comprising a plastid expressing the marker and DNA regions of homology to the genome of the plastid and optionally a ribosome binding site joined to the promoter. By methods using such constructs high levels of eukaryotic peptides, such as mammalian proteins, are produced in a plant cell by growing plant cells under conditions whereby the DNA encoding sequences are expressed to produce eukaryotic peptide in said plastid.

8. 发明授权

US06781033B2 Method for the transformation of plant cell plastids 有权
标题翻译：植物细胞质体转化方法
公开(公告)号：US06781033B2
公开(公告)日：2004-08-24
申请号：US09843324
申请日：2001-04-25
申请人： Jeffrey M Staub , Guangning Ye , Debra L. Broyles
发明人： Jeffrey M Staub , Guangning Ye , Debra L. Broyles
IPC分类号： A01H100
CPC分类号： C12N15/8214 , C12N15/8209 , C12N15/8275 , C12N15/8277
摘要： Novel compositions and methods useful for genetic engineering of plant cells to provide a method of producing plastid transformed plants are provided in the instant invention. In particular, the present invention provides methods for obtaining plastid transformed plants on medium containing plastid lethal compounds.
摘要翻译：在本发明中提供了用于植物细胞遗传工程以提供质量转化植物生产方法的新型组合物和方法。特别地，本发明提供了在含有质体致死化合物的培养基上获得质体转化植物的方法。

9. 发明授权

US06512162B2 Expression of eukaryotic peptides in plant plastids 失效
标题翻译：真核肽在植物质体中的表达
公开(公告)号：US06512162B2
公开(公告)日：2003-01-28
申请号：US09113244
申请日：1998-07-10
申请人： Kevin E. McBride , Narender Nehra , Douglas A. Russell , David M. Stalker , Jeffrey M. Staub
发明人： Kevin E. McBride , Narender Nehra , Douglas A. Russell , David M. Stalker , Jeffrey M. Staub
IPC分类号： C12N1582
CPC分类号： C12N15/8275 , C07K14/61 , C07K14/8117 , C07K2319/00 , C12N15/8214 , C12N15/8257
摘要： Constructs and methods are provided for expressing peptides derived from eukaryotic organisms in plant plastids. Constructs have a promoter functional in a plant plastid, a DNA sequence encoding a peptide derived from an eukaryotic organism and a transcription termination region. Other elements include a selectable marker for selection of plant cells comprising a plastid expressing the marker and DNA regions of homology to the genome of the plastid and optionally a ribosome binding site joined to the promoter. By methods using such constructs high levels of eukaryotic peptides, such as mammalian proteins, are produced in a plant cell by growing plant cells under conditions whereby the DNA encoding sequences re expressed to produce eukaryotic peptide in said plastid.
摘要翻译：提供构建体和方法用于表达在植物质体中衍生自真核生物的肽。构建体在植物质体中具有功能的启动子，编码来自真核生物的肽的DNA序列和转录终止区。其他元件包括用于选择植物细胞的选择性标记，其包括表达标记的质体和与质体的基因组同源的DNA区以及任选的与启动子连接的核糖体结合位点。通过使用这种构建的方法，通过在条件下生长植物细胞，在DNA质粒编码序列中再次表达以在所述质体中产生真核肽，从而在植物细胞中产生高水平的真核肽，例如哺乳动物蛋白质。

10. 发明授权

US06388168B1 DNA constructs and methods for stably transforming plastids of multicellular plants and expressing recombinant proteins therein 失效
标题翻译：用于稳定转化多细胞植物的质体并在其中表达重组蛋白质的DNA构建体和方法
公开(公告)号：US06388168B1
公开(公告)日：2002-05-14
申请号：US09193853
申请日：1998-11-18
申请人： Pal Maliga , Zora Svab Maliga , Jeffrey M. Staub
发明人： Pal Maliga , Zora Svab Maliga , Jeffrey M. Staub
IPC分类号： C12N1529
CPC分类号： C12N15/8214
摘要： DNA constructs are provided for stable transformation of plastids of multicellular plants and expression of foreign proteins in plastids. The DNA constructs comprise a transforming DNA which is targeted to a pre-determined location on the plastid genome and inserted into the plastid genome by homologous recombination with targeting segments comprising DNA sequences homologous to the pre-determined region of the plastid genome. The transforming DNA contains a non-lethal selectable marker gene which confers a selectable phenotype on cells having plastids in which substantially all of the genomes therein contain the transforming DNA (i.e., homoplasmic cells or tissues). The transforming DNA further comprises at least one insertion site 4 for an additional DNA segment, such as a gene encoding a protein for improving a characteristic of the transformed plant. The non-lethal selectable marker gene is preferably provided as a chimeric gene by assembly from an expression cassette comprising 5′ and 3′ regulatory segments, preferably derived from plastid genes. A coding segment encoding the non-lethal selectable marker is inserted between the 5′ and 3′ regulatory segments to form the chimeric gene. The non-lethal selectable marker coding segment preferred in the present invention is the coding region of aadA from bacteria, which encodes aminoglycoside 3″-adenylyltransferase to confer spectinomycin and streptomycin resistance.
摘要翻译：提供DNA构建体用于稳定转化多细胞植物的质体和外来蛋白质在质体中的表达。 DNA构建体包含转化DNA，其靶向质体基因组上的预定位置并通过与包含与质体基因组的预定区域同源的DNA序列的靶序列同源重组而插入质体基因组。转化DNA含有非致死性选择标记基因，其在具有质体的细胞上赋予可选择的表型，其中基本上所有的基因组中含有转化DNA（即，均质细胞或组织）。转化DNA还包含至少一个用于另外的DNA区段的插入位点4，例如编码用于改善转化植物特征的蛋白质的基因。非致死性选择标记基因优选通过从包含5'和3'调节区段的表达盒组装提供，优选来源于质体基因。将编码非致死性选择标记的编码区段插入5'和3'调节区段之间以形成嵌合基因。本发明优选的非致死选择性标记编码区是编码氨基糖苷3'-腺苷酰转移酶的细菌的aadA的编码区，赋予壮观霉素和链霉素抗性。

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