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    • 2. 发明申请
    • METHOD OF EFFICIENTLY ESTABLISHING INDUCED PLURIPOTENT STEM CELLS
    • 有效建立诱导型脑干细胞的方法
    • US20130183759A1
    • 2013-07-18
    • US13822906
    • 2011-09-14
    • Shinya YamanakaYoshinori YoshidaHidaka Yokota
    • Shinya YamanakaYoshinori YoshidaHidaka Yokota
    • C12N15/01
    • C12N15/00C12N5/0696C12N15/01C12N2501/998
    • The present invention provides a method of improving the efficiency of establishment of induced pluripotent stem (iPS) cells by inhibiting p38 function in the step of somatic cell nuclear reprogramming. The p38 function can be inhibited by bringing an inhibitor selected from the group consisting of (1) a chemical inhibitor of p38 (2) a dominant negative mutant of p38 or a nucleic acid that encodes the same, (3) a nucleic acid selected from the group consisting of siRNAs and shRNAs targeted to p38 and DNAs that encode the same and (4) an inhibitor of p38 pathway into contact with a somatic cell and the like. The present invention also provides an agent for improving the efficiency of establishment of induced pluripotent stem cells, which contains an inhibitor of p38 function, particularly an inhibitor selected from the group consisting of (1) a chemical inhibitor of p38 (2) a dominant negative mutant of p38 or a nucleic acid that encodes the same, (3) a nucleic acid selected from the group consisting of siRNAs and shRNAs targeted to p38 and DNAs that encode the same and (4) an inhibitor of p38 pathway. Moreover, the present invention provides a production method of iPS cells, which includes bringing a nuclear reprogramming substance and an inhibitor of p38 function into contact with a somatic cell.
    • 本发明提供了通过在体细胞核重编程步骤中抑制p38功能来提高诱导多能干细胞(iPS)细胞建立效率的方法。 可以通过使选自下述的抑制剂来抑制p38的功能:(1)p38的化学抑制剂(2)p38的显性失活突变体或其编码的核酸,(3)选自以下的核酸: 由针对p38的siRNA和shRNA组成的组和编码该p38的DNA和(4)p38途径的抑制剂与体细胞等接触的组。 本发明还提供了用于提高诱导多能干细胞的建立效率的试剂,其含有p38功能的抑制剂,特别是选自(1)p38(2)的显性阴性的化学抑制剂 p38的突变体或编码其的核酸,(3)选自靶向p38的siRNA和shRNA的核酸和编码其的DNA,以及(4)p38途径的抑制剂。 此外,本发明提供了iPS细胞的制备方法,其包括使核重编程物质和p38功能抑制剂与体细胞接触。
    • 6. 发明授权
    • Process for producing diol
    • 二醇生产方法
    • US4283579A
    • 1981-08-11
    • US72991
    • 1979-09-06
    • Yoshinori YoshidaHiroshi Oka
    • Yoshinori YoshidaHiroshi Oka
    • C07C27/00C07C27/02C07C29/09C07C29/128C07C29/80C07C31/20C07C33/035C07C67/00C07C67/03
    • C07C29/80C07C29/095C07C29/1285C07C67/03
    • A process for producing a diol by reacting an acetic ester of butanediol or butenediol with methanol, which comprises (a) continuously feeding to a first reaction-distilling column from its upper part a liquid acetic ester of butanediol or butenediol, while continuously feeding methanol to said column from its lower part, to bring both feeds into counter current gas-liquid contact in the presence of an acidic or a basic catalyst, to allow both feeds to react, and withdrawing from the bottom a bottom stream comprising a diol as major constituent, (b) continuously feeding to a hydrolyzer the distillate obtained from said first reaction-distilling column containing methyl acetate as major constituent, thus bringing said distillate into contact with water or steam in the presence of an acidic catalyst to hydrolyze the methyl acetate, (c) then continuously feeding the hydrolysis product thus obtained from the hydrolyzer to a second distilling column, withdrawing a methyl acetate-containing methanol stream from the top of said second distilling column, and recycling said stream to the lower part of the first reaction-distilling column. According to this process, it is possible to leave out the conventional complicated procedure necessary for the separation of methanol and, hence, to save the labor required therefor.
    • 一种通过使丁二醇或丁烯二醇的乙酸酯与甲醇反应来生产二醇的方法,该方法包括(a)从其上部连续向第一反应蒸馏塔中加入丁二醇或丁烯二醇的液体乙酸酯,同时连续地将甲醇加入到 所述柱从其下部分,在酸性或碱性催化剂的存在下将两种进料进入逆流气液接触,以允许两种进料反应,并从底部排出包含二醇作为主要成分的底部物流 ,(b)向水解器中连续地将由含有乙酸甲酯的第一反应 - 蒸馏塔获得的馏出物作为主要成分进料,由此在酸性催化剂的存在下使所述馏出物与水或蒸汽接触以水解乙酸甲酯( c)然后将由此获得的水解产物的水解产物连续地进料到第二蒸馏塔中,抽出含乙酸甲酯 甲醇流从所述第二蒸馏塔的顶部,并将所述物流再循环到第一反应蒸馏塔的下部。 根据该方法,可以省略分离甲醇所需的常规复杂程序,从而节省了所需的劳动。