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    • 1. 发明授权
    • Methodology for detection, enumeration, propagation and manipulation of bacteriophages
    • 检测,计数,繁殖和操纵噬菌体的方法
    • US08377678B2
    • 2013-02-19
    • US12309670
    • 2007-07-27
    • Jonathan RothJames LarkinFu-Chih HsuChoi-Iok Rebecca WongDavid A. BattigelliGeoffrey N. Roth
    • Jonathan RothJames LarkinFu-Chih HsuChoi-Iok Rebecca WongDavid A. BattigelliGeoffrey N. Roth
    • C12N1/00C12N1/20C12N7/00
    • C12Q1/04C12Q1/045
    • A method to propagate, enumerate and quantify bacteriophage(s) in a water sample or other aqueous sample was designed which contains ingredients to stimulate the growth of select bacterial species which are susceptible to infection by specific bacteriophage(s), in which interfering background organisms are either inhibited or inconsequential. Important features of the medium include oxidation-reduction compounds producing colored and/or fluorescent products, chromogenic and/or fluorogenic enzyme substrates, and temperature-independent gelling agent(s). A preferred combination is the growth medium containing 2,3,5-triphenyl tetrazolium chloride, 5-bromo-4-chloro-3-indolyl-B-D-galactoside, and appropriate gelling agents, which (when properly used) produces a dark red bacterial lawn containing teal blue-green, irregularly circular spots representing individual phage plaque, all discernible to the eye in visible light. The procedure can also be readily applied towards automatic counting systems under artificial illumination. The procedure can be employed with water samples and with elution buffers that can retain bacteriophages in suspension following contact by the buffer with foods, soils, hard surfaces and other solids that may be contaminated by bacteriophages.
    • 设计了一种在水样品或其他含水样品中繁殖,枚举和定量噬菌体的方法,其包含刺激特定噬菌体感染的选择细菌物种生长的成分,其中干扰背景生物 被抑制或无关紧要。 培养基的重要特征包括产生有色和/或荧光产物的氧化还原化合物,显色和/或荧光酶底物和与温度无关的胶凝剂。 优选的组合是含有2,3,5-三苯基四唑氯化物,5-溴-4-氯-3-吲哚基-β-半乳糖苷和适当的胶凝剂的生长培养基(当适当使用时)产生深红色细菌 草坪含有蓝绿色,不规则圆形的斑点,代表单个噬菌体斑块,在可见光下都可以看出眼睛。 该程序也可以方便地应用于人造照明下的自动计数系统。 该程序可以与水样和洗脱缓冲液一起使用,洗脱缓冲液可以在缓冲液与食物,土壤,硬表面和可能被噬菌体污染的其他固体接触后保持悬浮状态。
    • 3. 发明授权
    • Building construction assembly of structural modules
    • 建筑施工组装结构模块
    • US07797897B2
    • 2010-09-21
    • US10505558
    • 2003-02-26
    • Jonathan Roth
    • Jonathan Roth
    • E04B1/38
    • E04B1/12E04B1/6158E04B1/6175E04C2/22E04C2/34
    • A building construction assembly composed of factory-manufactured thermoplastic structural modules which when interconnected at a building site then create the entire shell of an edifice having side walls and a roof. Each module which is formed of a pair of parallel panels bridged by transverse webs is provided on either side along its length with a pair of flexible catch flanges projecting from the panels. When assembling these modules to create the shell of the edifice, adjacent modules are interconnected in coplanar relation by a double-faced joiner on each face of which is a pair of teeth. When one face of the joiner is pressed against a side of a module, its catch flanges then flex to snap into and behind the teeth to connect the joiner to this module. The other face of the joiner is similarly connected to the side of an adjacent module whereby the modules are then interconnected in coplanar relation. When it becomes necessary to interconnect four modules in a cross formation, use is then made of a square, quad-faced joiner on each face of which are a pair of teeth which are engageable by the catch flanges projecting from the side of the module to be joined to this face.
    • 由工厂制造的热塑性结构模块组成的建筑施工组件,当在建筑工地相互连接时,建造具有侧壁和屋顶的大厦的整个外壳。 由横向腹板桥接的一对平行面板形成的每个模块在其长度的两侧设置有一对从面板突出的柔性捕获凸缘。 当组装这些模块以形成大厦的外壳时,相邻的模块通过双面连接器在共面关系中互相连接,双面接头在其每个面上是一对齿。 当连接器的一个面压靠在模块的一侧时,其卡扣凸缘然后弯曲以卡入到齿内和后面,以将连接器连接到该模块。 接合器的另一面类似地连接到相邻模块的侧面,由此模块以共面关系互连。 当需要在交叉形式中互连四个模块时,然后使用正方形的四面连接器,其中每个面都是一对齿,其可以通过从模块的侧面突出的捕获凸缘接合到 加入这个脸。
    • 4. 发明申请
    • Building construction assembly of structural modules
    • 建筑施工组装结构模块
    • US20050091932A1
    • 2005-05-05
    • US10505558
    • 2003-02-26
    • Jonathan Roth
    • Jonathan Roth
    • E04B1/12E04B1/61E04C2/22E04C2/34E04H1/00
    • E04B1/12E04B1/6158E04B1/6175E04C2/22E04C2/34
    • A building construction assembly composed of factory-manufactured thermoplastic structural modules which when interconnected at a building site then create the entire shell of an edifice having side walls and a roof. Each module which is formed of a pair of parallel panels bridged by transverse webs is provided on either side along its length with a pair of flexible catch flanges projecting from the panels. When assembling these modules to create the shell of the edifice, adjacent modules are interconnected in coplanar relation by a double-faced joiner on each face of which is a pair of teeth. When one face of the joiner is pressed against a side of a module, its catch flanges then flex to snap into and behind the teeth to connect the joiner to this module. The other face of the joiner is similarly connected to the side of an adjacent module whereby the modules are then interconnected in coplanar relation. When it becomes necessary to interconnect four modules in a cross formation, use is then made of a square, quad-faced joiner on each face of which are a pair of teeth which are engageable by the catch flanges projecting from the side of the module to be joined to this face.
    • 由工厂制造的热塑性结构模块组成的建筑施工组件,当在建筑工地相互连接时,建造具有侧壁和屋顶的大厦的整个外壳。 由横向腹板桥接的一对平行面板形成的每个模块在其长度的两侧设置有一对从面板突出的柔性捕获凸缘。 当组装这些模块以形成大厦的外壳时,相邻的模块通过双面连接器在共面关系中互相连接,双面接头在其每个面上是一对齿。 当连接器的一个面压靠在模块的一侧时,其卡扣凸缘然后弯曲以卡入到齿内和后面,以将连接器连接到该模块。 接合器的另一面类似地连接到相邻模块的侧面,由此模块以共面关系互连。 当需要在交叉形式中互连四个模块时,然后使用正方形的四面连接器,其中每个面都是一对齿,其可以通过从模块的侧面突出的捕获凸缘接合到 加入这个脸。
    • 7. 发明授权
    • Computer-implemented cloud-based litigation management system
    • 计算机实施的云端诉讼管理系统
    • US08560367B2
    • 2013-10-15
    • US13369766
    • 2012-02-09
    • Jonathan RothAaron Yaffa
    • Jonathan RothAaron Yaffa
    • G06Q10/06
    • G06F17/30011
    • A system for managing litigation documents including a computer-readable medium having a litigation management application. The application has computer-executable instructions for managing documents. The system includes a server that executes the instructions for managing documents on the computer-readable medium. The system has a database containing document information. The database is arranged with the server such that the server can access and modify document information in the database. The application includes a first documents tab that receives inputs from a first user to upload documents to the database for display in the first documents tab. The application has a second documents tab that receives inputs from a second user. The application generates a notification for delivery to the second user when the first user uploads a document to the database. The application can display the document in the second documents tab when the first user uploads the document to the database.
    • 一种用于管理诉讼文件的系统,包括具有诉讼管理应用的计算机可读介质。 该应用程序具有用于管理文档的计算机可执行指令。 该系统包括执行用于管理计算机可读介质上的文档的指令的服务器。 该系统具有包含文档信息的数据库。 数据库与服务器一起安排,使得服务器可以访问和修改数据库中的文档信息。 应用程序包括第一文档选项卡,其接收来自第一用户的输入以将文档上传到数据库以在第一文档选项卡中显示。 应用程序具有从第二用户接收输入的第二文档选项卡。 当第一个用户将文档上传到数据库时,应用程序将生成一个用于传送给第二个用户的通知。 当第一个用户将文档上传到数据库时,应用程序可以在第二个文档选项卡中显示该文档。
    • 8. 发明申请
    • Test media and quantiative or qualitative method for identification and differentiation of biological materials in a test sample
    • 用于测试样品中生物材料鉴定和分化的测试介质和量化或定性方法
    • US20050196825A1
    • 2005-09-08
    • US11096908
    • 2005-04-01
    • Geoffrey RothJonathan Roth
    • Geoffrey RothJonathan Roth
    • C12Q1/04C12Q1/10C12Q1/16
    • C12Q1/04C12Q1/045C12Q1/10C12Q2334/00C12Q2334/50G01N2333/245G01N2333/25G01N2333/255G01N2333/924Y02A50/451
    • A test medium and method for detecting, quantifying, identifying and differentiating up to four (4) separate biological materials in a test sample. A test medium is disclosed which allows quantifying and differentiating under ambient light aggregates of biological entities producing specific enzymes, which might include general coliforms, E. coli, Aeromonas, and Salmonella in a single test medium. A new class of nonchromogenic substrates is disclosed which produce a substantially black, non-diffusible precipitate. This precipitate is not subject to interference from other chromogenic substrates present in the test medium. In one embodiment, the substrates are selected such that E. coli colonies present in the test medium show as substantially black, general coliforms colonies show in the test medium as a blue-violet color, Aeromonas colonies present in the test medium show as a generally red-pink color, and Salmonella colonies show as a generally teal-green color. Other microorganisms and color possibilities for detection and quantification thereof are also disclosed. An inhibitor and method for making a test medium incorporating the inhibitor are disclosed.
    • 用于在测试样品中检测,定量,鉴定和区分多达四(4)种分离的生物材料的测试介质和方法。 公开了一种测试介质,其允许在单个测试介质中产生特定酶的生物实体的环境光聚集体(其可以包括一般大肠杆菌,大肠杆菌,气单胞菌属和沙门氏菌)进行定量和鉴别。 公开了一类新的非致冷底物,其产生基本上黑色的非扩散性沉淀物。 该沉淀物不受来自测试介质中存在的其它显色底物的干扰。 在一个实施方案中,选择底物使得存在于测试培养基中的大肠杆菌菌落显示为基本上黑色,一般大肠菌群在测试培养基中显示为蓝紫色,测试培养基中存在的气单胞菌菌落显示为一般 红色粉红色和沙门氏菌菌落呈普通绿绿色。 还公开了用于其检测和定量的其它微生物和颜色可能性。 公开了一种制备包含抑制剂的测试介质的抑制剂和方法。