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    • 1. 发明申请
    • Test media and quantiative or qualitative method for identification and differentiation of biological materials in a test sample
    • 用于测试样品中生物材料鉴定和分化的测试介质和量化或定性方法
    • US20050196825A1
    • 2005-09-08
    • US11096908
    • 2005-04-01
    • Geoffrey RothJonathan Roth
    • Geoffrey RothJonathan Roth
    • C12Q1/04C12Q1/10C12Q1/16
    • C12Q1/04C12Q1/045C12Q1/10C12Q2334/00C12Q2334/50G01N2333/245G01N2333/25G01N2333/255G01N2333/924Y02A50/451
    • A test medium and method for detecting, quantifying, identifying and differentiating up to four (4) separate biological materials in a test sample. A test medium is disclosed which allows quantifying and differentiating under ambient light aggregates of biological entities producing specific enzymes, which might include general coliforms, E. coli, Aeromonas, and Salmonella in a single test medium. A new class of nonchromogenic substrates is disclosed which produce a substantially black, non-diffusible precipitate. This precipitate is not subject to interference from other chromogenic substrates present in the test medium. In one embodiment, the substrates are selected such that E. coli colonies present in the test medium show as substantially black, general coliforms colonies show in the test medium as a blue-violet color, Aeromonas colonies present in the test medium show as a generally red-pink color, and Salmonella colonies show as a generally teal-green color. Other microorganisms and color possibilities for detection and quantification thereof are also disclosed. An inhibitor and method for making a test medium incorporating the inhibitor are disclosed.
    • 用于在测试样品中检测,定量,鉴定和区分多达四(4)种分离的生物材料的测试介质和方法。 公开了一种测试介质,其允许在单个测试介质中产生特定酶的生物实体的环境光聚集体(其可以包括一般大肠杆菌,大肠杆菌,气单胞菌属和沙门氏菌)进行定量和鉴别。 公开了一类新的非致冷底物,其产生基本上黑色的非扩散性沉淀物。 该沉淀物不受来自测试介质中存在的其它显色底物的干扰。 在一个实施方案中,选择底物使得存在于测试培养基中的大肠杆菌菌落显示为基本上黑色,一般大肠菌群在测试培养基中显示为蓝紫色,测试培养基中存在的气单胞菌菌落显示为一般 红色粉红色和沙门氏菌菌落呈普通绿绿色。 还公开了用于其检测和定量的其它微生物和颜色可能性。 公开了一种制备包含抑制剂的测试介质的抑制剂和方法。