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1. 发明授权

US08377678B2 Methodology for detection, enumeration, propagation and manipulation of bacteriophages 有权
标题翻译：检测，计数，繁殖和操纵噬菌体的方法
公开(公告)号：US08377678B2
公开(公告)日：2013-02-19
申请号：US12309670
申请日：2007-07-27
申请人： Jonathan Roth , James Larkin , Fu-Chih Hsu , Choi-Iok Rebecca Wong , David A. Battigelli , Geoffrey N. Roth
发明人： Jonathan Roth , James Larkin , Fu-Chih Hsu , Choi-Iok Rebecca Wong , David A. Battigelli , Geoffrey N. Roth
IPC分类号： C12N1/00 , C12N1/20 , C12N7/00
CPC分类号： C12Q1/04 , C12Q1/045
摘要： A method to propagate, enumerate and quantify bacteriophage(s) in a water sample or other aqueous sample was designed which contains ingredients to stimulate the growth of select bacterial species which are susceptible to infection by specific bacteriophage(s), in which interfering background organisms are either inhibited or inconsequential. Important features of the medium include oxidation-reduction compounds producing colored and/or fluorescent products, chromogenic and/or fluorogenic enzyme substrates, and temperature-independent gelling agent(s). A preferred combination is the growth medium containing 2,3,5-triphenyl tetrazolium chloride, 5-bromo-4-chloro-3-indolyl-B-D-galactoside, and appropriate gelling agents, which (when properly used) produces a dark red bacterial lawn containing teal blue-green, irregularly circular spots representing individual phage plaque, all discernible to the eye in visible light. The procedure can also be readily applied towards automatic counting systems under artificial illumination. The procedure can be employed with water samples and with elution buffers that can retain bacteriophages in suspension following contact by the buffer with foods, soils, hard surfaces and other solids that may be contaminated by bacteriophages.
摘要翻译：设计了一种在水样品或其他含水样品中繁殖，枚举和定量噬菌体的方法，其包含刺激特定噬菌体感染的选择细菌物种生长的成分，其中干扰背景生物被抑制或无关紧要。培养基的重要特征包括产生有色和/或荧光产物的氧化还原化合物，显色和/或荧光酶底物和与温度无关的胶凝剂。优选的组合是含有2,3,5-三苯基四唑氯化物，5-溴-4-氯-3-吲哚基-β-半乳糖苷和适当的胶凝剂的生长培养基（当适当使用时）产生深红色细菌草坪含有蓝绿色，不规则圆形的斑点，代表单个噬菌体斑块，在可见光下都可以看出眼睛。该程序也可以方便地应用于人造照明下的自动计数系统。该程序可以与水样和洗脱缓冲液一起使用，洗脱缓冲液可以在缓冲液与食物，土壤，硬表面和可能被噬菌体污染的其他固体接触后保持悬浮状态。

2. 发明申请

US20100291539A1 METHODOLOGY FOR DETECTION, ENUMERATION, PROPAGATION AND MANIPULATION OF BACTERIOPHAGES 有权
标题翻译：检测，产生，传播和控制杀虫剂的方法
公开(公告)号：US20100291539A1
公开(公告)日：2010-11-18
申请号：US12309670
申请日：2007-07-27
申请人： Jonathan N. Roth , James Larkin , Fu-Chih Hsu , Choi-Iok Rebecca Wong , David A. Battigelli , Geoffrey N. Roth
发明人： Jonathan N. Roth , James Larkin , Fu-Chih Hsu , Choi-Iok Rebecca Wong , David A. Battigelli , Geoffrey N. Roth
IPC分类号： C12Q1/70
CPC分类号： C12Q1/04 , C12Q1/045
摘要： A method to propagate, enumerate and quantify bacteriophage(s) in a water sample or other aqueous sample was designed which contains ingredients to stimulate the growth of select bacterial species which are susceptible to infection by specific bacteriophage(s), in which interfering background organisms are either inhibited or inconsequential. Important features of the medium include oxidation-reduction compounds producing colored and/or fluorescent products, chromogenic and/or fluorogenic enzyme substrates, and temperature-independent gelling agent(s). A preferred combination is the growth medium containing 2,3,5-triphenyl tetrazolium chloride, 5-bromo-4-chloro-3-indolyl-B-D-galactoside, and appropriate gelling agents, which (when properly used) produces a dark red bacterial lawn containing teal blue-green, irregularly circular spots representing individual phage plaque, all discernible to the eye in visible light. The procedure can also be readily applied towards automatic counting systems under artificial illumination. The procedure can be employed with water samples and with elution buffers that can retain bacteriophages in suspension following contact by the buffer with foods, soils, hard surfaces and other solids that may be contaminated by bacteriophages.
摘要翻译：设计了一种在水样品或其他含水样品中繁殖，枚举和定量噬菌体的方法，其包含刺激特定噬菌体感染的选择细菌物种生长的成分，其中干扰背景生物被抑制或无关紧要。培养基的重要特征包括产生有色和/或荧光产物的氧化还原化合物，显色和/或荧光酶底物和与温度无关的胶凝剂。优选的组合是含有2,3,5-三苯基四唑氯化物，5-溴-4-氯-3-吲哚基-β-半乳糖苷和适当的胶凝剂的生长培养基（当适当使用时）产生深红色细菌草坪含有蓝绿色，不规则圆形的斑点，代表单个噬菌体斑块，在可见光下都可以看出眼睛。该程序也可以方便地应用于人造照明下的自动计数系统。该程序可以与水样和洗脱缓冲液一起使用，洗脱缓冲液可以在缓冲液与食物，土壤，硬表面和可能被噬菌体污染的其他固体接触后保持悬浮状态。

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