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1. 发明申请

US20060246556A1 Novel method for the production of polyunsaturated fatty acids 有权
标题翻译：用于生产多不饱和脂肪酸的新方法
公开(公告)号：US20060246556A1
公开(公告)日：2006-11-02
申请号：US10539891
申请日：2003-12-11
申请人： Johnathan Napier , Olga Sayanova , Colin Lazarus , Baoxiu Qi , Ernst Heinz , Thorsten Zank , Ulrich Zahringer
发明人： Johnathan Napier , Olga Sayanova , Colin Lazarus , Baoxiu Qi , Ernst Heinz , Thorsten Zank , Ulrich Zahringer
IPC分类号： C12P13/00 , C12P7/64
CPC分类号： C12N9/0083 , C12N9/1029 , C12N15/8247 , C12P7/6427 , C12P7/6472
摘要： The present invention relates to an improved process for the specific production of poly-unsaturated omega-3 and omega-6 fatty acids and a process for the production of triglycerides having an increased content of unsaturated fatty acids, in particular omega-3 and omega-6 fatty acids having at least two double bonds and a 20 or 22 carbon atom chain length. The invention relates to the production of a transgenic organism, preferably a transgenic plant or a transgenic microorganism, hav-ing an increased content of fatty acids, oils or lipids containing C20- or C22-fatty acids with a delta-5, 7, 8, 10 double bond, respectively due to the expression of a delta-8-desaturase and a delta-9-elon-gase from organisms such as plants preferably Algae like Isochrysis galbana or Euglena gracilis. In addition the invention relates to a process for the production of poly unsaturated fatty acids such as Eicosapentaenoic, Arachidonic, Docosapentaenoic or Doosahexaenoic acid through the co-expression of a delta-8-desaturase, a delta-9-elongase and a delta-5 desaturase in organisms such as microorganisms or plants. The invention additionally relates to the use of specific nucleic acid sequences encoding for the aforementioned proteins with delta-8-desaturase-, delta-9-elongase- or delta-5-desaturase-activity, nucleic acid constructs, vectors and organisms containing said nucleic acid sequences. The invention further relates to unsaturated fatty acids and triglycerides having an increased content of at least 1% by weight of unsaturated fatty acids and use thereof.
摘要翻译：本发明涉及一种用于特异性生产多不饱和ω-3和ω-6脂肪酸的改进方法和一种具有增加的不饱和脂肪酸含量，特别是ω-3和ω- 具有至少两个双键和20或22个碳原子链长的6种脂肪酸。本发明涉及转基因生物，优选转基因植物或转基因微生物的生产，其具有增加的含有C20-C22-脂肪酸的脂肪酸，油或脂质含量，其中Δ-5,7,8 ，10双键，分别由于诸如植物优选藻类如等鞭毛藻或小球虫的生物体的δ-8-去饱和酶和δ-9-脱氢酶的表达。此外，本发明涉及通过共同表达δ-8-去饱和酶，δ-9-延伸酶和δ-5的多不饱和脂肪酸如二十碳五烯酸，花生四烯酸，多糖五烯酸或多沙六酸，微生物或植物等生物体中的去饱和酶。本发明还涉及编码上述蛋白质的特定核酸序列与δ-8-去饱和酶，Δ-9-延伸酶或δ-5-去饱和酶活性，核酸构建体，载体和含有所述核酸的生物体的用途酸序列。本发明还涉及不饱和脂肪酸和甘油三酸酯，其具有增加的至少1重量％的不饱和脂肪酸的含量及其用途。

2. 发明授权

US07714185B2 Method for the production of polyunsaturated fatty acids 有权
标题翻译：生产多不饱和脂肪酸的方法
公开(公告)号：US07714185B2
公开(公告)日：2010-05-11
申请号：US10539891
申请日：2003-12-11
申请人： Jonathan A. Napier , Olga Sayanova , Colin M. Lazarus , Baoxiu Qi , Ernst Heinz , Thorsten Zank , Ulrich Zähringer
发明人： Jonathan A. Napier , Olga Sayanova , Colin M. Lazarus , Baoxiu Qi , Ernst Heinz , Thorsten Zank , Ulrich Zähringer
IPC分类号： C12N15/82 , C12N15/87 , C07H21/04
CPC分类号： C12N9/0083 , C12N9/1029 , C12N15/8247 , C12P7/6427 , C12P7/6472
摘要： The present invention relates to an improved process for the specific production of poly-unsaturated omega-3 and omega-6 fatty acids and a process for the production of triglycerides having an increased content of unsaturated fatty acids, in particular omega-3 and omega-6 fatty acids having at least two double bonds and a 20 or 22 carbon atom chain length. The invention relates to the production of a transgenic organism, preferably a transgenic plant or a transgenic microorganism, hav-ing an increased content of fatty acids, oils or lipids containing C20- or C22-fatty acids with a delta-5, 7, 8, 10 double bond, respectively due to the expression of a delta-8-desaturase and a delta-9-elon-gase from organisms such as plants preferably Algae like Isochrysis galbana or Euglena gracilis. In addition the invention relates to a process for the production of poly unsaturated fatty acids such as Eicosapentaenoic, Arachidonic, Docosapentaenoic or Doosahexaenoic acid through the co-expression of a delta-8-desaturase, a delta-9-elongase and a delta-5 desaturase in organisms such as microorganisms or plants. The invention additionally relates to the use of specific nucleic acid sequences encoding for the aforementioned proteins with delta-8-desaturase-, delta-9-elongase- or delta-5-desaturase-activity, nucleic acid constructs, vectors and organisms containing said nucleic acid sequences. The invention further relates to unsaturated fatty acids and triglycerides having an increased content of at least 1% by weight of unsaturated fatty acids and use thereof.
摘要翻译：本发明涉及一种用于特异性生产多不饱和ω-3和ω-6脂肪酸的改进方法和一种具有增加的不饱和脂肪酸含量，特别是ω-3和ω- 具有至少两个双键和20或22个碳原子链长的6种脂肪酸。本发明涉及转基因生物，优选转基因植物或转基因微生物的生产，其具有增加的含有C20-C22-脂肪酸的脂肪酸，油或脂质含量，其中Δ-5,7,8 ，10双键，分别由于诸如植物优选藻类如等鞭毛藻或小球虫的生物体的δ-8-去饱和酶和δ-9-脱氢酶的表达。此外，本发明涉及通过共同表达δ-8-去饱和酶，δ-9-延伸酶和δ-5的多不饱和脂肪酸如二十碳五烯酸，花生四烯酸，多糖五烯酸或多沙六酸，微生物或植物等生物体中的去饱和酶。本发明还涉及编码上述蛋白质的特定核酸序列与δ-8-去饱和酶，Δ-9-延伸酶或δ-5-去饱和酶活性，核酸构建体，载体和含有所述核酸的生物体的用途酸序列。本发明还涉及不饱和脂肪酸和甘油三酸酯，其具有增加的至少1重量％的不饱和脂肪酸的含量及其用途。

3. 发明申请

US20070136892A1 Process for the production of delta5-unsaturated fatty acids in transgenic organisms 有权
标题翻译：在转基因生物体内生产δ5-不饱和脂肪酸的方法
公开(公告)号：US20070136892A1
公开(公告)日：2007-06-14
申请号：US11603557
申请日：2006-11-22
申请人： Thorsten Zank , Johnathan Napier , Olga Sayanova
发明人： Thorsten Zank , Johnathan Napier , Olga Sayanova
IPC分类号： A01H1/00 , C07H21/04 , C12P13/00 , C12P7/64 , C12N9/04 , C12N5/04
CPC分类号： C12N15/8247 , C12N9/0071
摘要： A process for the production of Δ5-unsaturated fatty acids in transgenic organisms comprises transforming an organism with nucleic acid encoding a Δ5-desaturase.
摘要翻译：用于在转基因生物体中生产Δ5-不饱和脂肪酸的方法包括用编码Δ5-去饱和酶的核酸转化生物体。

4. 发明申请

US20080160054A1 Novel elongase gene, and process for the preparation of polyunsaturated fatty acids 审中-公开
标题翻译：新型延伸酶基因，以及制备多不饱和脂肪酸的方法
公开(公告)号：US20080160054A1
公开(公告)日：2008-07-03
申请号：US11682269
申请日：2007-03-05
申请人： Ernst Heinz , Thorsten Zank , Ulrich Zahringer , Jens Lerchl , Andreas Renz
发明人： Ernst Heinz , Thorsten Zank , Ulrich Zahringer , Jens Lerchl , Andreas Renz
IPC分类号： A61K8/02 , C07H21/04 , C07K16/00 , C12N15/00 , C12N5/00 , A23D7/00 , C12N5/02 , C12P21/04 , G01N33/53 , A61K36/00
CPC分类号： C12N9/1029 , C12N15/8247 , C12P7/6427 , C12P7/6472
摘要： The invention relates to a novel elongase gene with the sequences stated in sequence SEQ ID NO:1, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 7 or their homologs, derivatives or analogs, to a gene construct comprising this gene or its homologs, derivatives and analogs, and to its use. The invention also relates to vectors or transgenic organisms comprising an elongase gene with the sequence SEQ ID NO:1, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 7 or its homologs, derivatives and analogs. The invention furthermore relates to the use of the elongase gene sequences alone or in combination with further elongases and/or further fatty acid biosynthesis genes. The present invention relates to a novel elongase gene with the sequence SEQ ID NO:1 or its homologs, derivatives and analogs.Furthermore, the invention relates to a process for the preparation of polyunsaturated fatty acids and to a process for introducing DNA into organisms which produce large amounts of oils and, in particular, oils with a high content of unsaturated fatty acids. Moreover, the invention relates to an oil and/or a fatty acid preparation with a higher content of polyunsaturated fatty acids with at least two double bonds and/or a triacylglycerol preparation with a higher content of polyunsaturated fatty acids with at least two double bonds.
摘要翻译：本发明涉及具有序列SEQ ID NO：1，SEQ ID NO：3，SEQ ID NO：5和SEQ ID NO：7所示序列的新型延伸酶基因或其同源物，衍生物或类似物，其包含该基因或其同系物，衍生物和类似物，以及其用途。本发明还涉及包含具有序列SEQ ID NO：1，SEQ ID NO：3，SEQ ID NO：5和SEQ ID NO：7的延伸酶基因或其同源物，衍生物和类似物的载体或转基因生物。本发明还涉及单独使用延伸酶基因序列或与其它延伸酶和/或其它脂肪酸生物合成基因组合的用途。本发明涉及具有序列SEQ ID NO：1或其同系物，衍生物和类似物的新型延伸酶基因。此外，本发明涉及一种制备多不饱和脂肪酸的方法，以及将DNA引入生产大量油，特别是具有高含量不饱和脂肪酸的油的生物体中的方法。此外，本发明涉及具有较高含量的具有至少两个双键的多不饱和脂肪酸的油和/或脂肪酸制剂和/或具有至少两个双键的具有较高含量的多不饱和脂肪酸的三酰基甘油制剂。

5. 发明授权

US07723574B2 Process for the production of Δ5-unsaturated fatty acids in transgenic organisms 有权
标题翻译：在转基因生物体内生产＆Dgr。5-不饱和脂肪酸的方法
公开(公告)号：US07723574B2
公开(公告)日：2010-05-25
申请号：US11603557
申请日：2006-11-22
申请人： Thorsten Zank , Johnathan A. Napier , Olga Sayanova
发明人： Thorsten Zank , Johnathan A. Napier , Olga Sayanova
IPC分类号： A01H5/00 , C12N15/82 , C07H21/04
CPC分类号： C12N15/8247 , C12N9/0071
摘要： A process for the production of Δ5-unsaturated fatty acids in transgenic organisms comprises transforming an organism with nucleic acid encoding a Δ5-desaturase.
摘要翻译：用于在转基因生物体中生产＆Dgr。5-不饱和脂肪酸的方法包括用编码“去饱和酶”的核酸转化生物体。

6. 发明授权

US07537920B2 Method for the production of polyunsaturated fatty acids 有权
标题翻译：生产多不饱和脂肪酸的方法
公开(公告)号：US07537920B2
公开(公告)日：2009-05-26
申请号：US10547447
申请日：2004-01-29
申请人： Andreas Renz , Ernst Heinz , Amine Abbadi , Frederic Domergue , Thorsten Zank
发明人： Andreas Renz , Ernst Heinz , Amine Abbadi , Frederic Domergue , Thorsten Zank
IPC分类号： C12N9/12 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12N15/8247 , C12N9/1029 , C12P7/6427 , C12P7/6472
摘要： The present invention relates to a process for producing polyunsaturated fatty acids in an organism by introducing nucleic acids into the organism which code for polypeptides having acyl-CoA:lysophospholipid a cyltransferase activity. Advantageously, these nucleic acid sequences may, if appropriate together with further nucleic acid sequences coding for biosynthesis polypeptides of the fatty acid or lipid metabolism, be expressed in the transgenic organism. The invention furthermore relates to the nucleic acid sequences, to nucleic acid constructs comprising the nucleic acid sequences of the invention, to vectors comprising the nucleic acid sequences and/or the nucleic acid constructs and to transgenic organisms comprising the abovementioned nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced by the process of the invention and to their use.
摘要翻译：本发明涉及通过将核酸引入生物体中来生产多不饱和脂肪酸的方法，该生物体编码具有酰基-CoA：溶血磷脂一羧酰基转移酶活性的多肽。有利地，如果合适的话，这些核酸序列可以与编码脂肪酸或脂质代谢的生物合成多肽的其它核酸序列一起在转基因生物中表达。本发明还涉及核酸序列，包含本发明的核酸序列的核酸构建体，包含核酸序列和/或核酸构建体的载体以及包含上述核酸序列的转基因生物，核酸构建体和/或载体。本发明的另一部分涉及通过本发明的方法生产的油，脂和/或脂肪酸及其用途。

7. 发明授权

US08486671B2 Method for the production of polyunsaturated fatty acids 有权
公开(公告)号：US08486671B2
公开(公告)日：2013-07-16
申请号：US12417171
申请日：2009-04-02
申请人： Andreas Renz , Ernst Heinz , Amine Abbadi , Frederic Domergue , Thorsten Zank
发明人： Andreas Renz , Ernst Heinz , Amine Abbadi , Frederic Domergue , Thorsten Zank
IPC分类号： C12P7/64 , C12N9/12
CPC分类号： C12N15/8247 , C12N9/1029 , C12P7/6427 , C12P7/6472
摘要： The present invention relates to a process for producing polyunsaturated fatty acids in an organism by introducing nucleic acids into the organism which code for polypeptides having acyl-CoA:lysophospholipid a cyltransferase activity. Advantageously, these nucleic acid sequences may, if appropriate together with further nucleic acid sequences coding for biosynthesis polypeptides of the fatty acid or lipid metabolism, be expressed in the transgenic organism. The invention furthermore relates to the nucleic acid sequences, to nucleic acid constructs comprising the nucleic acid sequences of the invention, to vectors comprising the nucleic acid sequences and/or the nucleic acid constructs and to transgenic organisms comprising the abovementioned nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced by the process of the invention and to their use.

8. 发明申请

US20050214761A1 Novel elongase gene and method for producing polyunsaturated fatty acids 有权
标题翻译：新型延伸酶基因及多不饱和脂肪酸的制备方法
公开(公告)号：US20050214761A1
公开(公告)日：2005-09-29
申请号：US10502083
申请日：2003-01-13
申请人： Jens Lerchl , Ernst Heinz , Thorsten Zank
发明人： Jens Lerchl , Ernst Heinz , Thorsten Zank
IPC分类号： A01H5/00 , A23D9/00 , A23K1/16 , A61K8/30 , A61K8/36 , A61K31/201 , A61K31/202 , A61P9/00 , A61P29/00 , C12N9/00 , C12N9/10 , C12N15/09 , C12N15/82 , C12P7/64 , C12Q1/68 , C07H21/04 , C12N15/74
CPC分类号： C12P7/6427 , C12N9/1029 , C12N15/8247 , C12P7/6472
摘要： The invention relates to a novel elongase gene with the sequence SEQ ID NO:1 or its homologs, derivatives or analogs, to a gene construct comprising this gene or its homologs, derivatives and analogs, and to its use. The invention also relates to vectors or organisms comprising an elongase gene with the sequence SEQ ID NO:1 or its homologs, derivatives or analogs. Furthermore, the invention relates to a process for the preparation of polyunsaturated fatty acids and to a process for introducing DNA into organisms which produce large amounts of oils and, in particular, oils with a high content of unsaturated fatty acids. Moreover, the invention relates to an oil and/or a fatty acid preparation with a higher content of polyunsaturated fatty acids with at least two double bonds and/or a triacylglycerol preparation with a higher content of polyunsaturated fatty acids with at least two double bonds.
摘要翻译：本发明涉及具有序列SEQ ID NO：1或其同源物，衍生物或类似物的新型延伸酶基因，涉及包含该基因或其同系物，衍生物和类似物的基因构建体及其用途。本发明还涉及包含具有序列SEQ ID NO：1的延伸酶基因或其同源物，衍生物或类似物的载体或生物体。此外，本发明涉及一种制备多不饱和脂肪酸的方法，以及将DNA引入生产大量油，特别是具有高含量不饱和脂肪酸的油的生物体中的方法。此外，本发明涉及具有较高含量的具有至少两个双键的多不饱和脂肪酸的油和/或脂肪酸制剂和/或具有至少两个双键的具有较高含量的多不饱和脂肪酸的三酰基甘油制剂。

9. 发明授权

US08933300B2 Elongase gene, and process for the preparation of polyunsaturated fatty acids 有权
标题翻译：延长酶基因，以及制备多不饱和脂肪酸的方法
公开(公告)号：US08933300B2
公开(公告)日：2015-01-13
申请号：US11682269
申请日：2007-03-05
申请人： Ernst Heinz , Thorsten Zank , Ulrich Zähringer , Jens Lerchl , Andreas Renz
发明人： Ernst Heinz , Thorsten Zank , Ulrich Zähringer , Jens Lerchl , Andreas Renz
IPC分类号： C12N15/82 , A01H5/00 , C12N1/20 , C07H21/04 , C12N9/10 , C12P7/64
CPC分类号： C12N9/1029 , C12N15/8247 , C12P7/6427 , C12P7/6472
摘要： The invention relates to novel elongase genes with the sequences stated in sequence SEQ ID NO:1, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 7 or their homologs, derivatives or analogs, to a gene construct comprising this gene or its homologs, derivatives and analogs, and to its use. The invention also relates to vectors or transgenic organisms comprising an elongase gene with the sequence SEQ ID NO:1, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 7 or its homologs, derivatives and analogs. The invention furthermore relates to the use of the elongase gene sequences alone or in combination with further elongases and/or further fatty acid biosynthesis genes. The present invention relates to a novel elongase gene with the sequence SEQ ID NO:1 or its homologs, derivatives and analogs. Furthermore, the invention relates to a process for the preparation of polyunsaturated fatty acids and to a process for introducing DNA into organisms which produce large amounts of oils and, in particular, oils with a high content of unsaturated fatty acids. Moreover, the invention relates to an oil and/or a fatty acid preparation with a higher content of polyunsaturated fatty acids with at least two double bonds and/or a triacylglycerol preparation with a higher content of polyunsaturated fatty acids with at least two double bonds.
摘要翻译：本发明涉及具有序列SEQ ID NO：1，SEQ ID NO：3，SEQ ID NO：5和SEQ ID NO：7的序列的新型延伸酶基因或其同源物，衍生物或类似物，其包含该基因构建体基因或其同系物，衍生物和类似物及其用途。本发明还涉及包含具有序列SEQ ID NO：1，SEQ ID NO：3，SEQ ID NO：5和SEQ ID NO：7的延伸酶基因或其同源物，衍生物和类似物的载体或转基因生物。本发明还涉及单独使用延伸酶基因序列或与其它延伸酶和/或其它脂肪酸生物合成基因组合的用途。本发明涉及具有序列SEQ ID NO：1或其同系物，衍生物和类似物的新型延伸酶基因。此外，本发明涉及一种制备多不饱和脂肪酸的方法，以及将DNA引入生产大量油，特别是具有高含量不饱和脂肪酸的油的生物体中的方法。此外，本发明涉及具有较高含量的具有至少两个双键的多不饱和脂肪酸的油和/或脂肪酸制剂和/或具有至少两个双键的具有较高含量的多不饱和脂肪酸的三酰基甘油制剂。

10. 发明申请

US20100212034A1 Method For Producing Unsaturated Omega-3-Fatty Acids In Transgenic Organisms 有权
标题翻译：在转基因生物中生产不饱和ω-3-脂肪酸的方法
公开(公告)号：US20100212034A1
公开(公告)日：2010-08-19
申请号：US12768227
申请日：2010-04-27
申请人： Petra Cirpus , Jörg Bauer , Thorsten Zank , Ernst Heinz
发明人： Petra Cirpus , Jörg Bauer , Thorsten Zank , Ernst Heinz
IPC分类号： C07H21/04 , C12N15/63 , A01K67/027 , A01H5/00 , C12N1/00
CPC分类号： C12N9/0071 , C12N9/0004 , C12N15/8247
摘要： The present invention relates to nucleic acid sequences coding for polypeptides with ω-3-desaturase activity. The invention furthermore relates to nucleic acid constructs, vectors and organisms comprising at least one nucleic acid sequence according to the invention, at least one vector comprising the nucleic acid sequence and/or the nucleic acid constructs, and transgenic organisms comprise the abovementioned nucleic acid sequences, nucleic acid constructs and/or vectors.
摘要翻译：本发明涉及编码具有ω-3-去饱和酶活性的多肽的核酸序列。本发明还涉及包含至少一个本发明的核酸序列的核酸构建体，载体和生物体，至少一种包含核酸序列和/或核酸构建体的载体，转基因生物体包含上述核酸序列，核酸构建体和/或载体。

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