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1. 发明授权

US08373024B2 Method for producing unsaturated ω-3-fatty acids in transgenic organisms 有权
标题翻译：在转基因生物体内生产不饱和ω-3-脂肪酸的方法
公开(公告)号：US08373024B2
公开(公告)日：2013-02-12
申请号：US12768227
申请日：2010-04-27
申请人： Petra Cirpus , Jörg Bauer , Thorsten Zank , Ernst Heinz
发明人： Petra Cirpus , Jörg Bauer , Thorsten Zank , Ernst Heinz
IPC分类号： A01H5/00 , C12N15/82 , C12N1/20 , C07H21/04
CPC分类号： C12N9/0071 , C12N9/0004 , C12N15/8247
摘要： The present invention relates to nucleic acid sequences coding for polypeptides with ω-3-desaturase activity. The invention furthermore relates to nucleic acid constructs, vectors and organisms comprising at least one nucleic acid sequence according to the invention, at least one vector comprising the nucleic acid sequence and/or the nucleic acid constructs, and transgenic organisms comprise the abovementioned nucleic acid sequences, nucleic acid constructs and/or vectors.
摘要翻译：本发明涉及编码具有ω-3-去饱和酶活性的多肽的核酸序列。本发明还涉及包含至少一个本发明核酸序列的至少一种核酸构建体，载体和生物体，至少一种包含核酸序列和/或核酸构建体的载体，转基因生物体包含上述核酸序列，核酸构建体和/或载体。

2. 发明申请

US20100212034A1 Method For Producing Unsaturated Omega-3-Fatty Acids In Transgenic Organisms 有权
标题翻译：在转基因生物中生产不饱和ω-3-脂肪酸的方法
公开(公告)号：US20100212034A1
公开(公告)日：2010-08-19
申请号：US12768227
申请日：2010-04-27
申请人： Petra Cirpus , Jörg Bauer , Thorsten Zank , Ernst Heinz
发明人： Petra Cirpus , Jörg Bauer , Thorsten Zank , Ernst Heinz
IPC分类号： C07H21/04 , C12N15/63 , A01K67/027 , A01H5/00 , C12N1/00
CPC分类号： C12N9/0071 , C12N9/0004 , C12N15/8247
摘要： The present invention relates to nucleic acid sequences coding for polypeptides with ω-3-desaturase activity. The invention furthermore relates to nucleic acid constructs, vectors and organisms comprising at least one nucleic acid sequence according to the invention, at least one vector comprising the nucleic acid sequence and/or the nucleic acid constructs, and transgenic organisms comprise the abovementioned nucleic acid sequences, nucleic acid constructs and/or vectors.
摘要翻译：本发明涉及编码具有ω-3-去饱和酶活性的多肽的核酸序列。本发明还涉及包含至少一个本发明的核酸序列的核酸构建体，载体和生物体，至少一种包含核酸序列和/或核酸构建体的载体，转基因生物体包含上述核酸序列，核酸构建体和/或载体。

3. 发明授权

US09433228B2 Method for the production of multiple-unsaturated fatty acids in transgenic organisms 有权
公开(公告)号：US09433228B2
公开(公告)日：2016-09-06
申请号：US10566944
申请日：2004-07-16
申请人： Thorsten Zank , Jörg Bauer , Petra Cirpus , Amine Abbadi , Ernst Heinz , Xiao Qiu , Patricia Vrinten , Petra Sperling , Frederic Domergue , Astrid Meyer , Jelena Kirsch
发明人： Thorsten Zank , Jörg Bauer , Petra Cirpus , Amine Abbadi , Ernst Heinz , Xiao Qiu , Patricia Vrinten , Petra Sperling , Frederic Domergue , Astrid Meyer , Jelena Kirsch
IPC分类号： C12N15/82 , A23D9/00 , C12N9/02 , C12N9/10 , C12P7/64
CPC分类号： C12N15/8247 , A23D9/00 , A23D9/02 , A23L33/115 , A23V2002/00 , C11B1/00 , C12N9/0071 , C12N9/0083 , C12N9/0093 , C12N9/1029 , C12P7/6409 , C12P7/6427 , C12P7/6463 , C12P7/6472 , C12Y114/19 , C12Y114/19003 , C12Y203/01199 , Y02P20/52
摘要： The invention relates to a method for the production of multiply-unsaturated fatty acids in an organism, into which nucleic acids have been introduced, which code for polypeptides with Δ-5 elongase activity. Said nucleic acid sequences, optionally with further nucleic acid sequences, coding for polypeptides for the biosynthesis of fatty acids and lipid metabolism, are advantageously expressed in the organism. Nucleic acid sequences coding for a Δ-6 desaturase, a Δ-5 desaturase, Δ-4 desaturase and/or Δ-6 elongase activity are particularly advantageous and, advantageously, said saturases and elongases are derived from Thalassiosira, Euglena or Ostreococcus. The invention further relates to a method for the production of oils and/or triacylglycerides with an increased content of long-chain, multiply-unsaturated fatty acids. A particular embodiment of the invention is a method for the production of unsaturated ω-3 fatty acids and a method for the production of triglycerides with an increased content of unsaturated fatty acids, in particular, of Δ-3 fatty acids with more than three double bonds. Also disclosed is the production of a transgenic organism, preferably a transgenic plant, or a transgenic microorganism with increased content of ω-3 fatty acids, oils or lipids with ω-3 double bonds as a result of the expression of the elongases and desaturases employed in the above method, preferably in combination with ω-3 desaturases, for example a ω-3 desaturase from fungi of the family Pythiaceae such as the genus Phytophtora, for example, the genus and species Phytophtora infestans, or a ω-3 desaturase from algae such as the family Prasinophyceae, for example, the genus Ostreococcus and, particularly, the genus and species Ostreococcus tauri or diatomaceæ such as the genus Thalassiosira and, particularly, the genus and species Thalassiosira pseudonana. The invention also relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms containing the nucleic acid sequences and/or the nucleic acid constructs and transgenic organisms containing said nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced according to the above method and use thereof and, furthermore, unsaturated fatty acids and triglycerides with an increased content of unsaturated fatty acids and use thereof.

4. 发明授权

US07777098B2 Method for producing unsaturated ω-3-fatty acids in transgenic organisms 有权
标题翻译：在转基因生物体内生产不饱和ω-3-脂肪酸的方法
公开(公告)号：US07777098B2
公开(公告)日：2010-08-17
申请号：US10590958
申请日：2005-02-23
申请人： Petra Cirpus , Jörg Bauer , Thorsten Zank , Ernst Heinz
发明人： Petra Cirpus , Jörg Bauer , Thorsten Zank , Ernst Heinz
IPC分类号： C12N15/82 , C12N5/10 , C12N1/20
CPC分类号： C12N9/0071 , C12N9/0004 , C12N15/8247
摘要： The present invention relates to a process for production of unsaturated ω-3-fatty acids and to a process for production of triglycerides with an elevated content of unsaturated fatty acids, especially of ω-3-fatty acids having more than three double bonds. The invention relates to the production of a transgenic organism, preferably of a transgenic plant or of a transgenic microorganism, with an elevated content of unsaturated ω-3-fatty acids, oils or lipids having ω-3-double bonds as the result of the expression of an ω-3-desaturase from fungi of the family Pythiaceae such as the genus Phytophtora, for example of the genus and species Phytophtora infestans.
摘要翻译：本发明涉及一种不饱和ω-3-脂肪酸的制备方法以及具有不饱和脂肪酸含量升高的甘油三酯的制备方法，特别是具有三个以上双键的ω-3-脂肪酸。本发明涉及转基因生物，优选转基因植物或转基因微生物的生产，其具有升高的不饱和ω-3-脂肪酸含量，油或具有ω-3-双键的脂质作为来自真菌的真菌的ω-3-去饱和酶的表达，例如疫霉属（Phytophtora），例如属和种类疫霉属（Pytophtora infestans）。

5. 发明申请

US20080155705A1 Method For the Production of Multiple-Unsaturated Fatty Acids in Transgenic Organisms 有权
公开(公告)号：US20080155705A1
公开(公告)日：2008-06-26
申请号：US10566944
申请日：2004-07-16
申请人： Thorsten Zank , Jorg Bauer , Petra Cirpus , Amine Abbadi , Ernst Heinz , Xiao Qiu , Patricia Vrinten , Petra Sperling , Frederic Domergue , Astrid Meyer , Jelena Kirsch
发明人： Thorsten Zank , Jorg Bauer , Petra Cirpus , Amine Abbadi , Ernst Heinz , Xiao Qiu , Patricia Vrinten , Petra Sperling , Frederic Domergue , Astrid Meyer , Jelena Kirsch
IPC分类号： A01K67/00 , C12N15/82 , C07C51/00 , C12N15/11 , A01H5/00 , C12N15/00 , C12N1/20
CPC分类号： C12N15/8247 , A23D9/00 , A23D9/02 , A23L33/115 , A23V2002/00 , C11B1/00 , C12N9/0071 , C12N9/0083 , C12N9/0093 , C12N9/1029 , C12P7/6409 , C12P7/6427 , C12P7/6463 , C12P7/6472 , C12Y114/19 , C12Y114/19003 , C12Y203/01199 , Y02P20/52
摘要： The invention relates to a method for the production of multiply-unsaturated fatty acids in an organism, into which nucleic acids have been introduced, which code for polypeptides with Δ-5 elongase activity. Said nucleic acid sequences, optionally with further nucleic acid sequences, coding for polypeptides for the biosynthesis of fatty acids and lipid metabolism, are advantageously expressed in the organism. Nucleic acid sequences coding for a Δ-6 desaturase, a Δ-5 desaturase, Δ-4 desaturase and/or Δ-6 elongase activity are particularly advantageous and, advantageously, said saturases and elongases are derived from Thalassiosira, Euglena or Ostreococcus. The invention further relates to a method for the production of oils and/or triacylglycerides with an increased content of long-chain, multiply-unsaturated fatty acids. A particular embodiment of the invention is a method for the production of unsaturated ω-3 fatty acids and a method for the production of triglycerides with an increased content of unsaturated fatty acids, in particular, of Δ-3 fatty acids with more than three double bonds. Also disclosed is the production of a transgenic organism, preferably a transgenic plant, or a transgenic microorganism with increased content of ω-3 fatty acids, oils or lipids with ω-3 double bonds as a result of the expression of the elongases and desaturases employed in the above method, preferably in combination with ω-3 desaturases, for example a ω-3 desaturase from fungi of the family Pythiaceae such as the genus Phytophtora, for example, the genus and species Phytophtora infestans, or a ω-3 desaturase from algae such as the family Prasinophyceae, for example, the genus Ostreococcus and, particularly, the genus and species Ostreococcus tauri or diatomaceae such as the genus Thalassiosira and, particularly, the genus and species Thalassiosira pseudonana. The invention also relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms containing the nucleic acid sequences and/or the nucleic acid constructs and transgenic organisms containing said nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced according to the above method and use thereof and, furthermore, unsaturated fatty acids and triglycerides with an increased content of unsaturated fatty acids and use thereof.

6. 发明申请

US20070224661A1 Method for Producing Unsaturated Omega-3-Fatty Acids in Transgenic Organisms 有权
标题翻译：在转基因生物中生产不饱和ω-3-脂肪酸的方法
公开(公告)号：US20070224661A1
公开(公告)日：2007-09-27
申请号：US10590958
申请日：2005-02-23
申请人： Petra Cirpus , Jorg Bauer , Thorsten Zank , Ernst Heinz
发明人： Petra Cirpus , Jorg Bauer , Thorsten Zank , Ernst Heinz
IPC分类号： C12P1/04
CPC分类号： C12N9/0071 , C12N9/0004 , C12N15/8247
摘要： The present invention relates to a process for production of unsaturated ω-3-fatty acids and to a process for production of triglycerides with an elevated content of unsaturated fatty acids, especially of ω-3-fatty acids having more than three double bonds. The invention relates to the production of a transgenic organism, preferably of a transgenic plant or of a transgenic microorganism, with an elevated content of unsaturated ω-3-fatty acids, oils or lipids having ω-3-double bonds as the result of the expression of an ω-3-desaturase from fungi of the family Pythiaceae such as the genus Phytophtora, for example of the genus and species Phytophtora infestans. The invention furthermore relates to the nucleic acid sequences, nucleic acid constructs, vectors and organisms comprising at least one nucleic acid sequence according to the invention, at least one vector comprising the nucleic acid sequence and/or the nucleic acid constructs, and transgenic organisms comprise the abovementioned nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced by the process according to the invention, and to their use. The invention moreover relates to unsaturated fatty acids and triglycerides with an elevated content of unsaturated fatty acids and their use.
摘要翻译：本发明涉及一种生产不饱和ω-3-脂肪酸的方法，以及一种具有升高的不饱和脂肪酸含量，特别是具有三个以上双键的ω-3-脂肪酸含量的甘油三酯的制备方法。本发明涉及转基因生物，优选转基因植物或转基因微生物的生产，其具有升高的不饱和ω-3-脂肪酸含量，油或具有ω-3-双键的脂质作为来自真菌的真菌的ω-3-去饱和酶的表达，例如疫霉属（Phytophtora），例如属和种类疫霉属（Stytophtora infestans）。本发明还涉及包含至少一个根据本发明的核酸序列的核酸序列，核酸构建体，载体和生物体，至少一种包含核酸序列和/或核酸构建体的载体，以及转基因生物包括上述核酸序列，核酸构建体和/或载体。本发明的另一部分涉及通过根据本发明的方法生产的油，脂和/或脂肪酸及其用途。本发明还涉及不饱和脂肪酸和不饱和脂肪酸含量升高的甘油三酸酯及其用途。

7. 发明授权

US07629503B2 Δ-4 desaturases from Euglena gracilis, expressing plants, and oils containing PUFA 失效
标题翻译：来自Euglena gracilis的Delta-4去饱和酶，表达植物和含有PUFA的油
公开(公告)号：US07629503B2
公开(公告)日：2009-12-08
申请号：US10552127
申请日：2004-04-06
申请人： Petra Cirpus , Jörg Bauer , Astrid Meyer , Ernst Heinz , Ulrich Zähringer
发明人： Petra Cirpus , Jörg Bauer , Astrid Meyer , Ernst Heinz , Ulrich Zähringer
IPC分类号： A01H5/00 , C12N15/82 , C07H21/04
CPC分类号： C12N9/0071 , C12N15/8247
摘要： The present invention relates to an improved process for the specific preparation of unsaturated ω-3 fatty acids, and to a process for preparing triglycerides having an increased content of unsaturated fatty acids, particularly of ω-3 fatty acids having more than three double bonds. The invention relates to the preparation of a transgenic organism, preferably a transgenic plant or a transgenic microorganism, having increased content of fatty acids, oils or lipids having Δ-4 double bonds owing to the expression of a Δ-4-desaturase from Euglena gracilis. The invention additionally relates to expression cassettes comprising a nucleic acid sequence, a vector and organisms comprising at least one nucleic acid sequence or one expression cassette. The invention additionally relates to unsaturated fatty acids and to triglycerides having an increased content of unsaturated fatty acids and to the use thereof.Fatty acids and triglycerides have a large number of uses in the food industry, in animal nutrition, cosmetics and in the drugs sector. They are suitable for a wide variety of uses depending on whether they are free saturated or unsaturated fatty acids or triglycerides having an increased content of saturated or unsaturated fatty acids.
摘要翻译：本发明涉及用于特异性制备不饱和ω-3脂肪酸的改进方法，以及制备具有增加的不饱和脂肪酸含量，特别是具有多于三个双键的ω-3脂肪酸含量的甘油三酸酯的方法。本发明涉及一种转基因生物体，优选转基因植物或转基因微生物的制备，由于来自小眼球虫（Euglena gracilis）的Δ-4-去饱和酶的表达，具有增加的含有Δ-4双键的脂肪酸，油或脂质的含量。本发明还涉及包含核酸序列，载体和包含至少一个核酸序列或一个表达盒的生物体的表达盒。本发明还涉及不饱和脂肪酸和具有增加的不饱和脂肪酸含量的甘油三酸酯及其用途。脂肪酸和甘油三酯在食品工业，动物营养，化妆品和药物领域都有很多用途。它们适用于各种各样的用途，取决于它们是饱和还是不饱和脂肪酸的饱和或不饱和脂肪酸或饱和或不饱和脂肪酸含量增加的甘油三酸酯。

8. 发明申请

US20060218668A1 -4Desaturases from euglena gracilis, expressing plants, and oils containing pufa 失效
标题翻译： -4赤霉素，表达植物和含有普洱的油的去饱和酶
公开(公告)号：US20060218668A1
公开(公告)日：2006-09-28
申请号：US10552127
申请日：2004-04-06
申请人： Petra Cirpus , Jörg Bauer , Astrid Meyer , Ernst Heinz , Ulrich Zähringer
发明人： Petra Cirpus , Jörg Bauer , Astrid Meyer , Ernst Heinz , Ulrich Zähringer
IPC分类号： A01H1/00 , C12P7/64 , C12N9/04 , C12N15/82 , C12N5/04
CPC分类号： C12N9/0071 , C12N15/8247
摘要： The present invention relates to an improved process for the specific preparation of unsaturated ω-3 fatty acids, and to a process for preparing triglycerides having an increased content of unsaturated fatty acids, particularly of ω-3 fatty acids having more than three double bonds. The invention relates to the preparation of a transgenic organism, preferably a transgenic plant or a transgenic microorganism, having increased content of fatty acids, oils or lipids having Δ-4 double bonds owing to the expression of a Δ-4-desaturase from Euglena gracilis. The invention additionally relates to expression cassettes comprising a nucleic acid sequence, a vector and organisms comprising at least one nucleic acid sequence or one expression cassette. The invention additionally relates to unsaturated fatty acids and to triglycerides having an increased content of unsaturated fatty acids and to the use thereof. Fatty acids and triglycerides have a large number of uses in the food industry, in animal nutrition, cosmetics and in the drugs sector. They are suitable for a wide variety of uses depending on whether they are free saturated or unsaturated fatty acids or triglycerides having an increased content of saturated or unsaturated fatty acids.
摘要翻译：本发明涉及用于特异性制备不饱和ω-3脂肪酸的改进方法，以及制备具有增加的不饱和脂肪酸含量，特别是具有多于三个双键的ω-3脂肪酸含量的甘油三酸酯的方法。本发明涉及一种转基因生物体，优选转基因植物或转基因微生物的制备，由于来自小眼球虫（Euglena gracilis）的Δ-4-去饱和酶的表达，具有增加的含有Δ-4双键的脂肪酸，油或脂质的含量。本发明还涉及包含核酸序列，载体和包含至少一个核酸序列或一个表达盒的生物体的表达盒。本发明还涉及不饱和脂肪酸和具有增加的不饱和脂肪酸含量的甘油三酸酯及其用途。脂肪酸和甘油三酯在食品工业，动物营养，化妆品和药物领域都有很多用途。它们适用于各种各样的用途，取决于它们是饱和还是不饱和脂肪酸的饱和或不饱和脂肪酸或饱和或不饱和脂肪酸含量增加的甘油三酸酯。

9. 发明授权

US07871804B2 Method for producing polyunsaturated long-chain fatty acids in transgenic organisms 有权
标题翻译：在转基因生物体内生产多不饱和长链脂肪酸的方法
公开(公告)号：US07871804B2
公开(公告)日：2011-01-18
申请号：US11793854
申请日：2005-12-20
申请人： Petra Cirpus , Jörg Bauer , Ernst Heinz , Amine Abbadi , Jelena Kirsch
发明人： Petra Cirpus , Jörg Bauer , Ernst Heinz , Amine Abbadi , Jelena Kirsch
IPC分类号： C12N9/10 , C12N15/00 , C12N1/20 , C12P21/06 , C12P19/34 , C07H21/04 , C11B1/00 , A01H9/00
CPC分类号： C12N9/1029
摘要： The invention relates to a method for producing polyunsaturated fatty acids in an organism, according to which nucleic acids coding for polypeptides with an acyl-CoA:lysophospholipid-acyltransferase activity are introduced into the organism. Advantageously, the nucleic acid sequences can be expressed in the transgenic organism optionally together with other nucleic acid sequences coding for polypeptides of the fatty acid or lipid metabolism. The invention also relates to the inventive nucleic acid sequences, nucleic acid constructs containing the inventive nucleic acid sequences, vectors containing the inventive nucleic acid sequences and/or the nucleic acid constructs, and transgenic organisms containing the nucleic acid sequences, nucleic acid constructs and/or vectors. The invention further relates to oils, lipids and/or fatty acids produced according to the inventive method, and to the use of the same.
摘要翻译：本发明涉及在生物体中生产多不饱和脂肪酸的方法，根据该方法将编码具有酰基-CoA：溶血磷脂酰基转移酶活性的多肽的核酸引入生物体中。有利地，核酸序列可以在转基因生物任选地与编码脂肪酸或脂质代谢多肽的其它核酸序列一起表达。本发明还涉及本发明的核酸序列，含有本发明的核酸序列的核酸构建体，含有本发明核酸序列和/或核酸构建体的载体，以及含有核酸序列，核酸构建体和/ 或向量。本发明还涉及根据本发明方法生产的油，脂和/或脂肪酸及其用途。

10. 发明申请

US20090094707A1 Method for Producing Polyunsaturated Long-Chain Fatty Acids in Transgenic Organisms 有权
标题翻译：生物多不饱和长链脂肪酸在转基因生物中的应用
公开(公告)号：US20090094707A1
公开(公告)日：2009-04-09
申请号：US11793854
申请日：2005-12-20
申请人： Petra Cirpus , Jorg Bauer , Ernst Heinz , Amine Abbadi , Jelena Kirsch
发明人： Petra Cirpus , Jorg Bauer , Ernst Heinz , Amine Abbadi , Jelena Kirsch
IPC分类号： A01K67/027 , C12N15/11 , C12N9/12 , C12N15/00 , C11B1/00 , C12P7/64 , C12N1/21 , A01H5/00
CPC分类号： C12N9/1029
摘要： The invention relates to a method for producing polyunsaturated fatty acids in an organism, according to which nucleic acids coding for polypeptides with an acyl-CoA:lysophospholipid-acyltransferase activity am introduced into the organism. Advantageously, said nucleic acid sequences can be expressed in the transgenic organism optionally together with other nucleic acid sequences coding for polypeptides of the fatty acid or lipid metabolism. The invention also relates to the inventive nucleic acid sequences, nucleic acid constructs containing the inventive nucleic acid sequences, vectors containing the inventive nucleic acid sequences and/or the nucleic acid constructs, and transgenic organisms containing said nucleic acid sequences, nucleic acid constructs and/or vectors. The invention further relates to oils, lipids and/or fatty acids produced according to the inventive method, and to the use of the same.
摘要翻译：本发明涉及在生物体中生产多不饱和脂肪酸的方法，根据该方法将编码具有酰基-CoA：溶血磷脂酰基转移酶活性的多肽的核酸引入生物体中。有利地，所述核酸序列可以任选与编码脂肪酸或脂质代谢多肽的其它核酸序列一起在转基因生物体中表达。本发明还涉及本发明的核酸序列，含有本发明核酸序列的核酸构建体，含有本发明核酸序列和/或核酸构建体的载体，以及含有所述核酸序列，核酸构建体和/ 或向量。本发明还涉及根据本发明方法生产的油，脂和/或脂肪酸及其用途。

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