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1. 发明申请

US20070136892A1 Process for the production of delta5-unsaturated fatty acids in transgenic organisms 有权
标题翻译：在转基因生物体内生产δ5-不饱和脂肪酸的方法
公开(公告)号：US20070136892A1
公开(公告)日：2007-06-14
申请号：US11603557
申请日：2006-11-22
申请人： Thorsten Zank , Johnathan Napier , Olga Sayanova
发明人： Thorsten Zank , Johnathan Napier , Olga Sayanova
IPC分类号： A01H1/00 , C07H21/04 , C12P13/00 , C12P7/64 , C12N9/04 , C12N5/04
CPC分类号： C12N15/8247 , C12N9/0071
摘要： A process for the production of Δ5-unsaturated fatty acids in transgenic organisms comprises transforming an organism with nucleic acid encoding a Δ5-desaturase.
摘要翻译：用于在转基因生物体中生产Δ5-不饱和脂肪酸的方法包括用编码Δ5-去饱和酶的核酸转化生物体。

2. 发明申请

US20060246556A1 Novel method for the production of polyunsaturated fatty acids 有权
标题翻译：用于生产多不饱和脂肪酸的新方法
公开(公告)号：US20060246556A1
公开(公告)日：2006-11-02
申请号：US10539891
申请日：2003-12-11
申请人： Johnathan Napier , Olga Sayanova , Colin Lazarus , Baoxiu Qi , Ernst Heinz , Thorsten Zank , Ulrich Zahringer
发明人： Johnathan Napier , Olga Sayanova , Colin Lazarus , Baoxiu Qi , Ernst Heinz , Thorsten Zank , Ulrich Zahringer
IPC分类号： C12P13/00 , C12P7/64
CPC分类号： C12N9/0083 , C12N9/1029 , C12N15/8247 , C12P7/6427 , C12P7/6472
摘要： The present invention relates to an improved process for the specific production of poly-unsaturated omega-3 and omega-6 fatty acids and a process for the production of triglycerides having an increased content of unsaturated fatty acids, in particular omega-3 and omega-6 fatty acids having at least two double bonds and a 20 or 22 carbon atom chain length. The invention relates to the production of a transgenic organism, preferably a transgenic plant or a transgenic microorganism, hav-ing an increased content of fatty acids, oils or lipids containing C20- or C22-fatty acids with a delta-5, 7, 8, 10 double bond, respectively due to the expression of a delta-8-desaturase and a delta-9-elon-gase from organisms such as plants preferably Algae like Isochrysis galbana or Euglena gracilis. In addition the invention relates to a process for the production of poly unsaturated fatty acids such as Eicosapentaenoic, Arachidonic, Docosapentaenoic or Doosahexaenoic acid through the co-expression of a delta-8-desaturase, a delta-9-elongase and a delta-5 desaturase in organisms such as microorganisms or plants. The invention additionally relates to the use of specific nucleic acid sequences encoding for the aforementioned proteins with delta-8-desaturase-, delta-9-elongase- or delta-5-desaturase-activity, nucleic acid constructs, vectors and organisms containing said nucleic acid sequences. The invention further relates to unsaturated fatty acids and triglycerides having an increased content of at least 1% by weight of unsaturated fatty acids and use thereof.
摘要翻译：本发明涉及一种用于特异性生产多不饱和ω-3和ω-6脂肪酸的改进方法和一种具有增加的不饱和脂肪酸含量，特别是ω-3和ω- 具有至少两个双键和20或22个碳原子链长的6种脂肪酸。本发明涉及转基因生物，优选转基因植物或转基因微生物的生产，其具有增加的含有C20-C22-脂肪酸的脂肪酸，油或脂质含量，其中Δ-5,7,8 ，10双键，分别由于诸如植物优选藻类如等鞭毛藻或小球虫的生物体的δ-8-去饱和酶和δ-9-脱氢酶的表达。此外，本发明涉及通过共同表达δ-8-去饱和酶，δ-9-延伸酶和δ-5的多不饱和脂肪酸如二十碳五烯酸，花生四烯酸，多糖五烯酸或多沙六酸，微生物或植物等生物体中的去饱和酶。本发明还涉及编码上述蛋白质的特定核酸序列与δ-8-去饱和酶，Δ-9-延伸酶或δ-5-去饱和酶活性，核酸构建体，载体和含有所述核酸的生物体的用途酸序列。本发明还涉及不饱和脂肪酸和甘油三酸酯，其具有增加的至少1重量％的不饱和脂肪酸的含量及其用途。

3. 发明申请

US20050089865A1 Delta 6 desaturases from primulaceae, expressing plants and pufa-containing oils 有权
标题翻译：来自prim科的Delta 6去饱和酶，表达植物和含pufa的油
公开(公告)号：US20050089865A1
公开(公告)日：2005-04-28
申请号：US10504424
申请日：2003-02-06
申请人： Johnathan Napier , Olga Sayanova
发明人： Johnathan Napier , Olga Sayanova
IPC分类号： A01H5/00 , A01K67/027 , A23D9/00 , A23K1/16 , A61K8/30 , A61K8/36 , A61K8/37 , A61K31/202 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/02 , C12N15/09 , C12N15/29 , C12N15/53 , C12N15/82 , C12P7/64 , C12Q1/68 , A01H1/00 , C07H21/04 , C12N5/04 , C12N9/04
CPC分类号： C12N9/0083 , C12N15/8247 , C12P7/6427 , C12P7/6472
摘要： The present invention relates to an improved method for the specific production of unsaturated ω-3 fatty acids and a method for the production of triglycerides having an increased content of unsaturated fatty acids, in particular ω-3 fatty acids having more than three double bonds. The invention relates to the production of a transgenic organism, preferably a transgenic plant or a transgenic microorganism, having an increased content of fatty acids, oils or lipids having Δ 6 double bonds due to the expression of a Δ 6-desaturase from Primulaceae. The invention additionally relates to expression cassettes containing a nucleic acid sequence, a vector and organisms containing at least one nucleic acid sequence or an expression cassette. The invention further relates to unsaturated fatty acids and triglycerides having an increased content of unsaturated fatty acids and use thereof.
摘要翻译：本发明涉及用于特异性生产不饱和ω-3脂肪酸的改进方法以及生产具有增加的不饱和脂肪酸含量，特别是具有多于三个双键的ω-3脂肪酸的甘油三酸酯的方法。本发明涉及具有增加的含有Delta 6双键的脂肪酸，油或脂质含量的转基因植物或转基因微生物的转基因生物，这是由于来自洋甘菊科的Delta6-去饱和酶的表达。本发明还涉及含有核酸序列，载体和含有至少一个核酸序列或表达盒的生物体的表达盒。本发明还涉及具有增加的不饱和脂肪酸含量的不饱和脂肪酸和甘油三酸酯及其用途。

4. 发明授权

US07714185B2 Method for the production of polyunsaturated fatty acids 有权
标题翻译：生产多不饱和脂肪酸的方法
公开(公告)号：US07714185B2
公开(公告)日：2010-05-11
申请号：US10539891
申请日：2003-12-11
申请人： Jonathan A. Napier , Olga Sayanova , Colin M. Lazarus , Baoxiu Qi , Ernst Heinz , Thorsten Zank , Ulrich Zähringer
发明人： Jonathan A. Napier , Olga Sayanova , Colin M. Lazarus , Baoxiu Qi , Ernst Heinz , Thorsten Zank , Ulrich Zähringer
IPC分类号： C12N15/82 , C12N15/87 , C07H21/04
CPC分类号： C12N9/0083 , C12N9/1029 , C12N15/8247 , C12P7/6427 , C12P7/6472
摘要： The present invention relates to an improved process for the specific production of poly-unsaturated omega-3 and omega-6 fatty acids and a process for the production of triglycerides having an increased content of unsaturated fatty acids, in particular omega-3 and omega-6 fatty acids having at least two double bonds and a 20 or 22 carbon atom chain length. The invention relates to the production of a transgenic organism, preferably a transgenic plant or a transgenic microorganism, hav-ing an increased content of fatty acids, oils or lipids containing C20- or C22-fatty acids with a delta-5, 7, 8, 10 double bond, respectively due to the expression of a delta-8-desaturase and a delta-9-elon-gase from organisms such as plants preferably Algae like Isochrysis galbana or Euglena gracilis. In addition the invention relates to a process for the production of poly unsaturated fatty acids such as Eicosapentaenoic, Arachidonic, Docosapentaenoic or Doosahexaenoic acid through the co-expression of a delta-8-desaturase, a delta-9-elongase and a delta-5 desaturase in organisms such as microorganisms or plants. The invention additionally relates to the use of specific nucleic acid sequences encoding for the aforementioned proteins with delta-8-desaturase-, delta-9-elongase- or delta-5-desaturase-activity, nucleic acid constructs, vectors and organisms containing said nucleic acid sequences. The invention further relates to unsaturated fatty acids and triglycerides having an increased content of at least 1% by weight of unsaturated fatty acids and use thereof.
摘要翻译：本发明涉及一种用于特异性生产多不饱和ω-3和ω-6脂肪酸的改进方法和一种具有增加的不饱和脂肪酸含量，特别是ω-3和ω- 具有至少两个双键和20或22个碳原子链长的6种脂肪酸。本发明涉及转基因生物，优选转基因植物或转基因微生物的生产，其具有增加的含有C20-C22-脂肪酸的脂肪酸，油或脂质含量，其中Δ-5,7,8 ，10双键，分别由于诸如植物优选藻类如等鞭毛藻或小球虫的生物体的δ-8-去饱和酶和δ-9-脱氢酶的表达。此外，本发明涉及通过共同表达δ-8-去饱和酶，δ-9-延伸酶和δ-5的多不饱和脂肪酸如二十碳五烯酸，花生四烯酸，多糖五烯酸或多沙六酸，微生物或植物等生物体中的去饱和酶。本发明还涉及编码上述蛋白质的特定核酸序列与δ-8-去饱和酶，Δ-9-延伸酶或δ-5-去饱和酶活性，核酸构建体，载体和含有所述核酸的生物体的用途酸序列。本发明还涉及不饱和脂肪酸和甘油三酸酯，其具有增加的至少1重量％的不饱和脂肪酸的含量及其用途。

5. 发明授权

US07723574B2 Process for the production of Δ5-unsaturated fatty acids in transgenic organisms 有权
标题翻译：在转基因生物体内生产＆Dgr。5-不饱和脂肪酸的方法
公开(公告)号：US07723574B2
公开(公告)日：2010-05-25
申请号：US11603557
申请日：2006-11-22
申请人： Thorsten Zank , Johnathan A. Napier , Olga Sayanova
发明人： Thorsten Zank , Johnathan A. Napier , Olga Sayanova
IPC分类号： A01H5/00 , C12N15/82 , C07H21/04
CPC分类号： C12N15/8247 , C12N9/0071
摘要： A process for the production of Δ5-unsaturated fatty acids in transgenic organisms comprises transforming an organism with nucleic acid encoding a Δ5-desaturase.
摘要翻译：用于在转基因生物体中生产＆Dgr。5-不饱和脂肪酸的方法包括用编码“去饱和酶”的核酸转化生物体。

6. 发明授权

US08785727B2 Desaturase and method for the production of polyunsaturated fatty acids in transgenic organisms 有权
标题翻译：去饱和酶和在转基因生物体内生产多不饱和脂肪酸的方法
公开(公告)号：US08785727B2
公开(公告)日：2014-07-22
申请号：US12990492
申请日：2009-04-29
申请人： Joerg Bauer , Johnathan A. Napier , Olga Sayanova
发明人： Joerg Bauer , Johnathan A. Napier , Olga Sayanova
IPC分类号： C12P7/64 , C12N9/02 , C12N15/00 , C07H21/04 , A01H5/00 , C12N15/82 , C12N5/14 , A23K1/165 , C11B1/00
CPC分类号： C12N15/8247 , C12N9/0083 , C12P7/6427 , C12P7/6436 , C12P7/6472 , C12P7/6481
摘要： The present invention relates to a polynucleotide from Emiliana huxleyi which codes for a desaturase and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotide according to the invention, and to the polypeptides encoded by the polynucleotide. The invention furthermore relates to antibodies against the polypeptide according to the invention. Finally, the invention also relates to production methods for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions and to their use as drugs, cosmetics, foodstuffs, feedstuffs, preferably fish food, or food supplements.
摘要翻译：本发明涉及编码去饱和酶的Emiliana huxleyi的多核苷酸，其可用于重组生产多不饱和脂肪酸。本发明还涉及包含根据本发明的多核苷酸的载体，宿主细胞和转基因非人生物以及由多核苷酸编码的多肽。本发明还涉及针对根据本发明的多肽的抗体。最后，本发明还涉及多不饱和脂肪酸和油，脂肪和脂肪酸组合物的制备方法以及它们作为药物，化妆品，食品，饲料，优选鱼食或食品补充剂的用途。

7. 发明申请

US20130097733A1 Acyltransferases and Uses Thereof in Fatty Acid Production 有权
标题翻译：脂肪酸生产中的酰基转移酶及其用途
公开(公告)号：US20130097733A1
公开(公告)日：2013-04-18
申请号：US13806269
申请日：2011-06-21
申请人： Toralf Senger , Laurent Marty , Sten Stymne , Jenny Lindberg Yilmaz , Johnathan A. Napier , Olga Sayanova , Richard Haslam , Ruiz Lopez Noemi
发明人： Toralf Senger , Laurent Marty , Sten Stymne , Jenny Lindberg Yilmaz , Johnathan A. Napier , Olga Sayanova , Richard Haslam , Ruiz Lopez Noemi
IPC分类号： C12P7/64 , C12N9/10
CPC分类号： C12N9/1029 , A23D9/02 , A23K20/158 , A23L33/105 , A23L33/115 , A23L33/12 , A23V2002/00 , A23V2250/1862 , A23V2250/1868 , A23V2250/187 , A23V2250/1882 , C11C1/045 , C12N15/8247 , C12P7/6427 , C12Y203/0102 , Y02P20/52
摘要： The present invention relates to the recombinant manufacture of polyunsaturated fatty acids. Specifically, it relates to acyltransferase polypeptides, polynucleotides encoding said acyltransferases as well as vectors, host cells, non-human transgenic organisms containing said polynucletides. Moreover, the present invention contemplates methods for the manufacture of polyunsaturated fatty acids as well as oils obtained by such methods.
摘要翻译：本发明涉及多不饱和脂肪酸的重组制造。具体地，它涉及酰基转移酶多肽，编码所述酰基转移酶的多核苷酸以及载体，宿主细胞，含有所述多核苷酸的非人转基因生物。此外，本发明考虑了制备多不饱和脂肪酸的方法以及通过这些方法获得的油。

8. 发明授权

US08017839B2 Process for the production of arachidonic acid and/or eicosapentaenoic acid 有权
标题翻译：用于生产花生四烯酸和/或二十碳五烯酸的方法
公开(公告)号：US08017839B2
公开(公告)日：2011-09-13
申请号：US12083300
申请日：2006-10-10
申请人： Johnathan A. Napier , Olga Sayanova
发明人： Johnathan A. Napier , Olga Sayanova
IPC分类号： A01H5/00 , C12N15/82 , C07H21/04
CPC分类号： C12N15/8247 , C12N9/0083 , C12N9/1029
摘要： The present invention relates to a new process for the production of arachidonic acid and/or eicosapentaenoic acid in plants through the co-expression of a Δ-12-/Δ-15-desaturase, Δ-9-elongase, Δ-8-desaturase and a Δ-5-desaturase and a process for the production of lipids or oils having an increased content of unsaturated fatty acids, in particular ω-3 and ω-6 fatty acids having at least two double bonds and a 18 or 20 carbon atom chain length. Preferably the arachidonic acid and eicosapentaenoic acid are produced in at least a 1:2 ratio. The invention furthermore relates to the production of a transgenic plants, preferably a transgenic crop plant, having an increased content of arachidonic acid and/or eicosapentaenoic acid, oils or lipids containing Ciβ- or C20-fatty acids with a double bond in position Δ 5, 8, 9, 11, 12, 14, 15 or dffthe fatty acid produced, respectively due to the expression of the Δ-12-/Δ-15-desaturase, of the Δ-9-elongase, of the Δ-8-desaturase and of the Δ-5-desaturase in the plant. The expression of the inventive Δ-12-/Δ-15-desaturase leads preferably to linoleic acid and linolenic acid as products having a double bond in the position Δ 9, 12 and 15 of the fatty acid. The invention additionally relates to specific nucleic acid sequences encoding for proteins with Δ-12-/Δ-15-desaturase-, Δ-9-elongase-, Δ-8-desaturase- or Δ-5-desaturase-activity, nucleic acid constructs, vectors and transgenic plants containing said nucleic acid sequences.
摘要翻译：本发明涉及一种通过共同表达植物中花生四烯酸和/或二十碳五烯酸生产花生四烯酸和/或二十碳五烯酸的新方法。 -8-去饱和酶和α-D-去饱和酶以及具有增加的不饱和脂肪酸含量，特别是具有至少两个双键的ω-3和ω-6脂肪酸的脂质或油的方法，以及 18或20个碳原子链长。优选花生四烯酸和二十碳五烯酸以至少1:2的比例制备。本发明还涉及具有增加的花生四烯酸和/或二十碳五烯酸含量的转基因植物，优选转基因作物植物的生产，所述转基因植物含有具有位置的双键的C 1-18或-C 20 - 脂肪酸＆Dgr ; 5，8，9，11，12，14，15或分别由于Dgr-9-延伸酶的表达而产生的脂肪酸，＆Dgr; -8去饱和酶和植物中的＆Dgr。-5-去饱和酶。本发明的Dgr-12 - /＆Dgr--15-去饱和酶的表达优选导致亚油酸和亚麻酸作为具有位置为＆Dgr的双键的产物; 9,12和15的脂肪酸。本发明另外涉及编码具有“Dgr”-12A / Dgr; -15-去饱和酶，＆Dgr; -9-延伸酶，＆Dgr; -8-去饱和酶或“Dgr”-5去饱和酶 - 活性，核酸构建体，载体和含有所述核酸序列的转基因植物。

9. 发明申请

US20090019559A1 Nucleic Acid 有权
标题翻译：核酸
公开(公告)号：US20090019559A1
公开(公告)日：2009-01-15
申请号：US12279560
申请日：2007-02-13
申请人： Johnathan A. Napier , Olga Sayanova , Monica Venegas Caleron
发明人： Johnathan A. Napier , Olga Sayanova , Monica Venegas Caleron
IPC分类号： C12N15/85 , C12N15/54 , A01K67/027 , A01H5/00 , C12N1/00 , C12N15/74 , C12N15/82
CPC分类号： C12N15/8247 , C12N9/0083 , C12N9/1029 , C12P7/6427 , C12P7/6472
摘要： The invention relates to nucleic acid derived from Perkinsus marinus which encodes a 9-elongase, a Δ8-desaturase and a Δ5-desaturase enzyme. All of the coding sequences can be transcribed as a single transcript.
摘要翻译：本发明涉及源自Perkinsus marinus的编码9-延伸酶，Δδ-去饱和酶和Δ5-去饱和酶的核酸。所有编码序列都可以转录为单个转录本。

10. 发明授权

US09493520B2 Fatty acid desaturases and elongases and uses thereof 有权
标题翻译：脂肪酸去饱和酶和延长酶及其用途
公开(公告)号：US09493520B2
公开(公告)日：2016-11-15
申请号：US13384277
申请日：2010-07-15
申请人： Jörg Bauer , Johnathan A. Napier , Olga Sayanova
发明人： Jörg Bauer , Johnathan A. Napier , Olga Sayanova
IPC分类号： C12N15/82 , C07H21/04 , C07K14/435
CPC分类号： C12N15/8247 , C07K14/435 , C12N9/0071 , C12N9/93 , C12Y114/19 , C12Y114/19003 , C12Y114/19004 , C12Y114/19006 , C12Y602/01003
摘要： The invention provides isolated nucleic acid molecules which encode novel fatty acid desaturases and elongases from the organism Emiliana huxleyi. The invention also provides recombinant expression vectors containing desaturase or elongase nucleic acid molecules, host cells into which the expression vectors have been introduced, and methods for large-scale production of long chain polyunsaturated fatty acids (LCPUFAs), e.g. arachidonic acid (ARA), eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA).
摘要翻译：本发明提供了分离的核酸分子，其编码来自生物Emiliana huxleyi的新型脂肪酸去饱和酶和延伸酶。本发明还提供了含有去饱和酶或延伸酶核酸分子的重组表达载体，已引入表达载体的宿主细胞，以及用于大规模生产长链多不饱和脂肪酸（LCPUFA）的方法。花生四烯酸（ARA），二十碳五烯酸（EPA）或二十二碳六烯酸（DHA）。

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