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1. 发明授权

US5464768A Enhanced nitrite production in transfected murine cells 失效
标题翻译：在转染的鼠细胞中增强亚硝酸盐的产生
公开(公告)号：US5464768A
公开(公告)日：1995-11-07
申请号：US243350
申请日：1994-05-16
申请人： Johnathan L. Kiel , Jill E. Parker , John G. Bruno
发明人： Johnathan L. Kiel , Jill E. Parker , John G. Bruno
IPC分类号： C12N9/02 , C12N15/85
CPC分类号： C12N9/0036
摘要： Mamammalian cell lines capable of enhanced nitrite production are by transfecting a murine macrophage or murine thymoma with barley nitrate reductase gene (NR).
摘要翻译：通过用大麦硝酸还原酶基因（NR）转染鼠巨噬细胞或鼠胸腺瘤，能够增强亚硝酸盐产生的Mamammalian细胞系。

2. 发明授权

US06303316B1 Organic semiconductor recognition complex and system 有权
标题翻译：有机半导体识别复杂系统
公开(公告)号：US06303316B1
公开(公告)日：2001-10-16
申请号：US09608706
申请日：2000-06-30
申请人： Johnathan L. Kiel , John G. Bruno , Jill E. Parker , John L. Alls , Charles R. Batishko , Eric A. Holwitt
发明人： Johnathan L. Kiel , John G. Bruno , Jill E. Parker , John L. Alls , Charles R. Batishko , Eric A. Holwitt
IPC分类号： C12Q168
CPC分类号： C12Q1/6825 , C12Q1/6837 , Y10T436/143333 , C12Q2565/607
摘要： In a recognition complex system, nucleic acid ligands comprising random DNA sequences are operatively coupled to an organic semiconductor and distributed so as to form an array of recognition complexes. When an unknown chemical or biological analyte is applied to the array, the electrical and/or photochemical properties of one or more of the recognition complexes are altered upon binding of the nucleic acid ligand to the analyte. The degree to which the electrical and/or photochemical properties change is a function of the affinity of the nucleic acid ligand sequence for the analyte. The electrical and photochemical changes associated with the array, as a whole, can be used as a unique signature to identify the analyte. In certain embodiments, an iterative process of selection and amplification of nucleic acid ligands that bind to the analyte can be used to generate a new array with greater affinity and specificity for a target analyte, or to produce one or more nucleic acid ligands with high binding affinity for an analyte. The present invention also provides methods for preparing nucleic acid ligands that bind with high affinity to an analyte and using such nucleic acid ligands to neutralize the analyte.
摘要翻译：在识别复合体系中，包含随机DNA序列的核酸配体可操作地偶联到有机半导体上，并分布以形成识别复合物的阵列。当将未知的化学或生物分析物施加到阵列时，一个或多个识别复合物的电和/或光化学性质在核酸配体与分析物结合时被改变。电和/或光化学性质变化的程度是核酸配体序列对分析物的亲和力的函数。与阵列相关联的电学和光化学变化作为一个整体，可以用作识别分析物的独特标记。在某些实施方案中，结合分析物的核酸配体的选择和扩增的迭代过程可用于产生对靶分析物具有更大亲和性和特异性的新阵列，或产生具有高结合力的一个或多个核酸配体对分析物的亲和力。本发明还提供了制备以高亲和力结合分析物并使用这种核酸配体中和分析物的核酸配体的方法。

3. 发明授权

US06878519B1 DNA probes for specific genes of anthrax 失效
标题翻译：针对炭疽特异性基因的DNA探针
公开(公告)号：US06878519B1
公开(公告)日：2005-04-12
申请号：US10339259
申请日：2003-01-06
申请人： Jill E. Parker , Johnathan L. Kiel
发明人： Jill E. Parker , Johnathan L. Kiel
IPC分类号： C07H21/04 , C12Q1/68
CPC分类号： C07H21/04 , C12Q1/686 , C12Q1/689
摘要： A PCR-based method for the identification of Bacillus anthracis is described. The method utilizes novel primer sets; designated 2Xlg3F (SEQ ID NO 3), 2Xlg3R (SEQ ID NO 4), 2Xlg3F2 (SEQ ID NO 5), 2Xlg3R2 (SEQ ID NO 6), 4XH1a2F (SEQ ID NO 7), and 4XH1a2R (SEQ ID NO 8).
摘要翻译：描述了用于鉴定炭疽芽孢杆菌的基于PCR的方法。该方法利用新型引物组; 命名为2X1g3F（SEQ ID NO3），2X1G3R（SEQ ID NO4），2X1g3F2（SEQ ID NO：5），2X1g3R2（SEQ ID NO：6），4XH1a2F（SEQ ID NO：7）和4XH1a2R（SEQ ID NO：8）。

4. 发明授权

US08092988B1 Medium and method of deriving and using a mutated bacteria strain 有权
标题翻译：培养基和衍生和使用突变菌株的方法
公开(公告)号：US08092988B1
公开(公告)日：2012-01-10
申请号：US11820039
申请日：2007-05-25
申请人： Jill E. Parker , Johnathan L. Kiel , Homer Gifford , John L. Alls , Pedro J. Morales, legal representative
发明人： Jill E. Parker , Johnathan L. Kiel , Homer Gifford , John L. Alls
IPC分类号： C12Q1/00 , C12N15/00
CPC分类号： C12R1/07 , A61K39/07 , A61K2039/522
摘要： A new strain of Bacillus anthracis derived from the Sterne vaccine strain of Bacillus anthracis by growth on a high-nitrate-concentration, 3-amino-L-tyrosine growth medium.
摘要翻译：通过在高硝酸盐浓度的3-氨基-L-酪氨酸生长培养基上生长，衍生自炭疽芽孢杆菌的Sterne疫苗株的新型芽孢杆菌菌株。

5. 发明授权

US5856108A Biosynthesis of diazomelanin and diazoluminomelanin and methods thereof 失效
标题翻译：重氮木兰素和二叠氮木兰素的生物合成及其方法
公开(公告)号：US5856108A
公开(公告)日：1999-01-05
申请号：US779694
申请日：1991-10-21
申请人： Johnathan L. Kiel , Jill E. Parker , Eric A. Holwitt , Harvey A. Schwertner
发明人： Johnathan L. Kiel , Jill E. Parker , Eric A. Holwitt , Harvey A. Schwertner
IPC分类号： C12N9/02 , C12P17/12 , C12Q1/12 , G01N33/58 , C12Q1/00 , C12Q1/04 , C12Q1/26
CPC分类号： C12N9/0036 , C12P17/12 , C12Q1/12 , G01N33/582
摘要： There is provided a method for producing diazoluminomelanin (DALM) which comprises culturing in a medium containing nitrate, 3-amino-L-tyrosine (3-AT) and luminol under suitable metabolic conditions, a microorganism containing nitrate reductase.Also provided is a method for directly detecting microorganisms containing nitrate reductase or those into which nitrate reductase can be introduced by recombinant DNA technology, which comprises culturing the microorganism in a medium containing nitrate, 3-amino-L-tyrosine (3-AT) and luminol under suitable metabolic conditions, transferring the medium to a microtiter plate or tube coated with antibody or an antiligand to which the microorganism would specifically bind, washing the plate or tube and activating luminescence.Further, there is provided a method for producing diazomelanin (DM) which comprises culturing in a medium containing nitrate and 3-amino-L-tyrosine (3-AT) under suitable metabolic conditions, a microorganism containing nitrate reductase.Yet further, there is provided a method for directly detecting microorganisms containing nitrate reductase or those into which nitrate reductase can be introduced by recombinant DNA technology, which comprises culturing the microorganism in a medium containing nitrate and 3-amino-L-tyrosine (3-AT) under suitable metabolic conditions, transferring the medium to a microtiter plate or tube coated with antibody or an antiligand to which the microorganism would specifically bind, washing the plate or tube, adding luminol and activating luminescence.
摘要翻译：提供了一种生产重氮酚蓝蛋白（DALM）的方法，该方法包括在合适的代谢条件下，在含硝酸盐，3-氨基-L-酪氨酸（3-AT）和鲁米诺的培养基中培养含有硝酸还原酶的微生物。还提供了一种用于直接检测含有硝酸还原酶的微生物的方法或其中可以通过重组DNA技术引入硝酸还原酶的方法，其包括在含有硝酸盐，3-氨基-L-酪氨酸（3-AT）和将鲁米诺转移到适当的代谢条件下，将培养基转移到用抗体或微生物将特异性结合的抗配体的微量滴定板或管，洗涤板或管并激活发光。此外，提供了一种生产重氮木兰素（DM）的方法，其包括在适当的代谢条件下在含有硝酸盐和3-氨基-L-酪氨酸（3-AT）的培养基中培养含有硝酸还原酶的微生物。此外，提供了一种直接检测含有硝酸还原酶的微生物的方法或其中可以通过重组DNA技术引入硝酸还原酶的方法，其包括在含有硝酸盐和3-氨基-L-酪氨酸（3- AT），将培养基转移到用抗体或微生物将特异性结合的抗配体的微量滴定板或管，洗涤板或管，加入鲁米诺并激活发光。

6. 发明授权

US6013520A Breast tumor cells for study of nonionizing radiation effects 失效
标题翻译：乳腺肿瘤细胞用于研究非离子辐射效应
公开(公告)号：US6013520A
公开(公告)日：2000-01-11
申请号：US140069
申请日：1998-08-20
申请人： Jill E. Parker , Johnathan L. Kiel
发明人： Jill E. Parker , Johnathan L. Kiel
IPC分类号： G01N33/574 , C12N5/10
CPC分类号： G01N33/57415
摘要： The cell line EMT-6 are transformed with the chromosomal insertion of the plasmid pSV.sub.2 neoNR10.sub.1, ATCC No. 69617. The transformed cells, EMT-6/pSV.sub.2 neoNR10.sub.1, produce diazoluminomelanin (DALM) intracellularly when provided with nitrate, luminol and 3-amino-L-tyrosine.multidot.HCl (3AT). The modified cells can be used to study mechanisms for radiofrequency and light radiation interactions with breast tumor cells in vitro and in mice. The effects of drugs, hormones, and cytokines that affect the expression of nitric oxide synthase and its activity can also be studied to understand the effects of these materials on breast tumor cells.
摘要翻译：细胞系EMT-6用质粒pSV2neoNR101，ATCC No.69617的染色体插入进行转化。转化细胞EMT-6 / pSV2neoNR101在提供硝酸盐，鲁米诺和3-氨基-L的细胞内产生二氮嘧啶糖素（DALM） - 酪氨酸HCl（3AT）。修饰的细胞可用于研究在体外和小鼠中与乳腺肿瘤细胞的射频和光辐射相互作用的机制。还可以研究影响一氧化氮合酶表达的药物，激素和细胞因子的作用及其活性，以了解这些材料对乳腺肿瘤细胞的影响。

7. 发明申请

US20120021004A1 MEDIUM AND METHOD OF DERIVING AND USING A MUTATED BACTERIA STRAIN 有权
标题翻译：中间体和使用一种突变细菌菌株的方法
公开(公告)号：US20120021004A1
公开(公告)日：2012-01-26
申请号：US11820039
申请日：2007-05-25
申请人： Jill E. Parker , Johnathan L. Kiel , Homer Gifford , John L. Alls , Pedro J. Morales
发明人： Jill E. Parker , Johnathan L. Kiel , Homer Gifford , John L. Alls , Pedro J. Morales
IPC分类号： A61K39/07 , A61P31/04 , C12P21/00 , A61P37/04 , C12N15/01 , C12N1/20
CPC分类号： C12R1/07 , A61K39/07 , A61K2039/522
摘要： A new strain of Bacillus anthracis derived from the Sterne vaccine strain of Bacillus anthracis by growth on a high-nitrate-concentration, 3-amino-L-tyrosine growth medium.
摘要翻译：通过在高硝酸盐浓度的3-氨基-L-酪氨酸生长培养基上生长，衍生自炭疽芽孢杆菌的Sterne疫苗株的新型芽孢杆菌菌株。

8. 发明授权

US06326196B1 Nitrate reductase-transfected HeLa cells for cancer and microwave bioeffects research 失效
标题翻译：硝酸还原酶转染的HeLa细胞用于癌症和微生物的生物效应研究
公开(公告)号：US06326196B1
公开(公告)日：2001-12-04
申请号：US09766017
申请日：2001-01-22
申请人： Jill E. Parker , Johnathan L. Kiel
发明人： Jill E. Parker , Johnathan L. Kiel
IPC分类号： C12N508
CPC分类号： G01N33/5011 , G01N33/5008 , G01N33/502
摘要： The cell line HeLa is transformed with the chromosomal insertion of the plasmid pSV2neoNR101, ATCC No. 69617. The transformed cells, HeLaNR1, produce diazoluminomelanin (DALM) intra cellularly when provided with nitrate, luminol and 3-amino-L-tyrosine•HC1 (3AT). The modified cells can be used to study mechanisms for radiofrequency and light radiation interactions with carcinoma of the cervix. The effects of drugs, hormones, and cytokines that affect the expression of nitric oxide synthase and its activity can also be studied to understand the effects of these materials on cervix cells.
摘要翻译：细胞系HeLa用质粒pSV2neoNR101，ATCC No.69617的染色体插入转化。当提供硝酸盐，鲁米诺和3-氨基-L-酪氨酸时，转化细胞HeLaNR1在细胞内产生二氮嘧啶蛋白（DALM） 3AT）。修饰的细胞可用于研究与子宫颈癌的射频和光辐射相互作用的机制。还可以研究影响一氧化氮合酶表达的药物，激素和细胞因子的作用及其活性，以了解这些材料对子宫颈细胞的影响。

9. 发明授权

US5156971A Rapid identification of environmental bacillus 失效
标题翻译：快速鉴定环境杆菌
公开(公告)号：US5156971A
公开(公告)日：1992-10-20
申请号：US821525
申请日：1992-01-15
申请人： Johnathan L. Kiel , John L. Alls , Richard A. Weber , Jill E. Parker
发明人： Johnathan L. Kiel , John L. Alls , Richard A. Weber , Jill E. Parker
IPC分类号： C12N1/20 , C12Q1/04
CPC分类号： C12R1/07 , C12N1/20 , C12Q1/04 , G01N2333/32
摘要： A diagnostic test for environmental bacillus which comprises the steps of inoculating an agar growth medium comprising a nitrate source, luminol and 3-amino-L-tyrosine (3AT) with the sample, incubating the inoculated medium and determining the presence of the bacillus. The novel medium preferably comprises potassium nitrate, luminol, 3-amino-L-tyrosine and trypticase soy agar. Antibiotics and/or a specific bacteriophage may be added to the medium surface in localized areas to show specific bacterial lysis for identification. The novel medium and the methods of this invention are suitable for the identification of B. anthracis.
摘要翻译：环境芽孢杆菌的诊断试验包括以下步骤：将含有硝酸盐源，鲁米诺和3-氨基-L-酪氨酸（3AT）的琼脂生长培养基与样品接种，培养接种的培养基并确定芽孢杆菌的存在。该新型培养基优选包含硝酸钾，鲁米诺，3-氨基-L-酪氨酸和胰蛋白酶大豆琼脂。可将抗生素和/或特异性噬菌体加入局部区域的培养基表面，以显示特异性细菌裂解进行鉴定。本发明的新型培养基和方法适用于鉴定炭疽杆菌。

10. 发明授权

US5972721A Immunomagnetic assay system for clinical diagnosis and other purposes 失效
标题翻译：免疫磁性检测系统用于临床诊断等目的
公开(公告)号：US5972721A
公开(公告)日：1999-10-26
申请号：US816429
申请日：1997-03-14
申请人： John G. Bruno , Johnathan L. Kiel , John P. Kilian
发明人： John G. Bruno , Johnathan L. Kiel , John P. Kilian
IPC分类号： B03C1/01 , B03C1/032 , B03C1/033 , B03C1/034 , G01N33/533 , B03C1/00
CPC分类号： B03C1/032 , B03C1/01 , B03C1/0332 , B03C1/034 , Y10S436/824
摘要： An apparatus and method for immunomagnetic separation and concentration of target biological materials is disclosed. The immunomagnetic separation is performed by a magnetic flow cell, or filter block, as part of an automated mostly continuous immunomagnetic assay system. The magnetic flow cell has two bundles of ferromagnetic rods or pins positioned inside an internal chamber so that a fluid sample flowing through the flow cell passes through the pins. A pair of cobalt magnets flank the flow cell so that the pins concentrate and sufficiently increase the magnetic fields so that even nanometer size magnetic beads can be captured. The overall system combines a reaction subsystem for reacting coated magnetic beads with a sample, a collection subsystem for capturing magnetic beads, a rinsing subsystem for removing debris and a filtering subsystem for removing captured magnetic beads from the collection subsystem. The new magnetic flow filter is the key component for the collection and filtering subsystems.
摘要翻译：公开了一种用于免疫磁性分离和靶向生物材料浓缩的装置和方法。免疫磁性分离由磁流量池或过滤块进行，作为自动化大多数连续的免疫磁性测定系统的一部分。磁流量计具有两束位于内部室内的铁磁棒或销，使得流过流动池的流体样品通过销。在流动池侧面的一对钴磁体，使得引脚集中并充分增加磁场，使得可以捕获甚至纳米尺寸的磁珠。整个系统结合了用于使涂覆的磁珠与样品反应的反应子系统，用于捕获磁珠的收集子系统，用于去除碎屑的冲洗子系统和用于从收集子系统中去除捕获的磁珠的过滤子系统。新的磁流过滤器是收集和过滤子系统的关键组件。

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