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1. 发明申请

US20120046176A1 NUCLEIC ACID SEQUENCING 有权
标题翻译：核酸序列
公开(公告)号：US20120046176A1
公开(公告)日：2012-02-23
申请号：US12860462
申请日：2010-08-20
申请人： Xing Su , Kai Wu , Liming Wang , Jianquan Liu , Grace M. Credo
发明人： Xing Su , Kai Wu , Liming Wang , Jianquan Liu , Grace M. Credo
IPC分类号： C40B20/00
CPC分类号： C12Q1/6874 , C12Q2531/125 , C12Q2535/122 , C12Q2565/301 , C12Q2565/607
摘要： Nucleic acid sequencing using concatemers of DNA is provided. Optionally, amplified reaction products from the repeated incorporation and excision of a nucleoside complementary to a nucleoside of the DNA to be sequenced onto primer molecules hybridized to the concatemers of DNA are detected. Nucleic acid sequencing using concatemers of DNA and non-natural oligonucleotides is also provided. Nucleic acid sequencing reactions are detected electronically and or optically using arrays of detectors.
摘要翻译：提供使用DNA连接子的核酸测序。任选地，检测来自重复掺入和切除与待测序的DNA的核苷互补的核苷酸的扩增反应产物与DNA连接体杂交的引物分子上。还提供了使用DNA和非天然寡核苷酸的并联体的核酸测序。使用检测器阵列电子和/或光学检测核酸测序反应。

2. 发明申请

US20100330553A1 Chemically induced optical signals and DNA sequencing 有权
标题翻译：化学诱导光信号和DNA测序
公开(公告)号：US20100330553A1
公开(公告)日：2010-12-30
申请号：US12459309
申请日：2009-06-30
申请人： Xing Su , Liming Wang , Jianquan Liu , Kai Wu
发明人： Xing Su , Liming Wang , Jianquan Liu , Kai Wu
IPC分类号： C12Q1/68 , C12M1/00 , G02B6/10
CPC分类号： G02B6/1221 , C12Q1/6874 , C12Q2565/607 , C12Q2563/107 , C12Q2527/125
摘要： Methods for sequencing nucleic acids are presented. Sequencing is accomplished through the chemical amplification of the products of DNA synthesis and the detection of the chemically amplified products. In embodiments of the invention, a substrate is provided having a plurality of molecules of DNA to be sequenced attached and a plurality of molecules capable of chelating pyrophosphate ions attached, the DNA molecules to be sequenced are primed, and a next complementary nucleotide is incorporated and excised a plurality of times leading to the buildup of pyrophosphate ions locally around the DNA molecule to be sequenced. Pyrophosphate ions are captured by the substrate-attached chelators and optically detected to determine the identity of the next complementary nucleic acid in the DNA molecule to be sequenced.
摘要翻译：介绍了核酸测序方法。通过化学扩增DNA合成产物和化学扩增产物的检测来完成测序。在本发明的实施方案中，提供了具有多个待测序的DNA分子的底物和能够螯合连接的焦磷酸根离子的多个分子，引入待测序的DNA分子，并且掺入下一个互补核苷酸，切除多次导致待测序的DNA分子局部周围的焦磷酸盐离子的积聚。焦磷酸根离子被底物附着的螯合剂捕获并光学检测以确定待测序的DNA分子中下一个互补核酸的同一性。

3. 发明授权

US08715932B2 Nucleic acid sequencing 有权
标题翻译：核酸测序
公开(公告)号：US08715932B2
公开(公告)日：2014-05-06
申请号：US12860462
申请日：2010-08-20
申请人： Xing Su , Kai Wu , Liming Wang , Jianquan Liu , Grace M. Credo
发明人： Xing Su , Kai Wu , Liming Wang , Jianquan Liu , Grace M. Credo
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q2531/125 , C12Q2535/122 , C12Q2565/301 , C12Q2565/607
摘要： Nucleic acid sequencing using concatemers of DNA is provided. Optionally, amplified reaction products from the repeated incorporation and excision of a nucleoside complementary to a nucleoside of the DNA to be sequenced onto primer molecules hybridized to the concatemers of DNA are detected. Nucleic acid sequencing using concatemers of DNA and non-natural oligonucleotides is also provided. Nucleic acid sequencing reactions are detected electronically and or optically using arrays of detectors.
摘要翻译：提供使用DNA连接子的核酸测序。任选地，检测来自重复掺入和切除与待测序的DNA的核苷互补的核苷酸的扩增反应产物与DNA连接体杂交的引物分子上。还提供了使用DNA和非天然寡核苷酸的并联体的核酸测序。使用检测器阵列电子和/或光学检测核酸测序反应。

4. 发明授权

US08674086B2 Nucleotides and oligonucleotides for nucleic acid sequencing 有权
标题翻译：用于核酸测序的核苷酸和寡核苷酸
公开(公告)号：US08674086B2
公开(公告)日：2014-03-18
申请号：US12823995
申请日：2010-06-25
申请人： Jianquan Liu , Xing Su , Kai Wu
发明人： Jianquan Liu , Xing Su , Kai Wu
IPC分类号： C07H19/20 , C07H19/00 , C07H19/04
CPC分类号： C07H19/10 , C12Q1/6874 , C12Q2521/101 , C12Q2521/319 , C12Q2521/525 , C12Q2525/186 , C12Q2537/157 , C12Q2565/537 , C12Q2565/607
摘要： Embodiments of the invention provide non-natural bifunctional nucleotides having both nuclease resistance and nucleic acid synthesis blocking properties and methods of sequencing nucleic acids that employ non-natural bifunctional nucleic acids. Additional embodiments provide non-natural oligonucleotides and methods for sequencing nucleic acids using the non-natural oligonucleotides. Methods according to embodiments of the invention employ electronic detection and fluorescent detection of nucleic acid sequencing reactions.
摘要翻译：本发明的实施方案提供具有核酸酶抗性和核酸合成阻断性质的非天然双功能核苷酸，以及使用非天然双功能核酸测序核酸的方法。另外的实施方案提供非天然寡核苷酸和使用非天然寡核苷酸测序核酸的方法。根据本发明的实施方案的方法使用核酸测序反应的电子检测和荧光检测。

5. 发明授权

US09671558B2 Chemically induced optical signals and DNA sequencing 有权
公开(公告)号：US09671558B2
公开(公告)日：2017-06-06
申请号：US12459309
申请日：2009-06-30
申请人： Xing Su , Liming Wang , Jianquan Liu , Kai Wu
发明人： Xing Su , Liming Wang , Jianquan Liu , Kai Wu
IPC分类号： C12Q1/68 , G02B6/122
CPC分类号： G02B6/1221 , C12Q1/6874 , C12Q2565/607 , C12Q2563/107 , C12Q2527/125
摘要： Methods for sequencing nucleic acids are presented. Sequencing is accomplished through the chemical amplification of the products of DNA synthesis and the detection of the chemically amplified products. In embodiments of the invention, a substrate is provided having a plurality of molecules of DNA to be sequenced attached and a plurality of molecules capable of chelating pyrophosphate ions attached, the DNA molecules to be sequenced are primed, and a next complementary nucleotide is incorporated and excised a plurality of times leading to the buildup of pyrophosphate ions locally around the DNA molecule to be sequenced. Pyrophosphate ions are captured by the substrate-attached chelators and optically detected to determine the identity of the next complementary nucleic acid in the DNA molecule to be sequenced.

6. 发明授权

US09551682B2 High throughput biochemical detection using single molecule fingerprinting arrays 有权
标题翻译：使用单分子指纹图谱的高通量生物化学检测
公开(公告)号：US09551682B2
公开(公告)日：2017-01-24
申请号：US13538346
申请日：2012-06-29
申请人： Oguz H. Elibol , Grace M. Credo , Xing Su , Madoo Varma , Jonathan S. Daniels , Drew Hall , Handong Li , Noureddine Tayebi , Kai Wu
发明人： Oguz H. Elibol , Grace M. Credo , Xing Su , Madoo Varma , Jonathan S. Daniels , Drew Hall , Handong Li , Noureddine Tayebi , Kai Wu
IPC分类号： G01N27/327 , B82Y15/00 , C40B20/04 , C40B50/16
CPC分类号： G01N27/3277 , B01J19/0046 , B01J2219/00608 , B01J2219/00653 , B01J2219/00722 , B81B7/04 , B81B2201/0214 , B81B2201/058 , B82Y15/00 , C40B20/04 , C40B50/16 , G01N27/3278 , G01N27/4145 , G01N27/4146 , G01N27/4148
摘要： Various embodiments provide devices, methods, and systems for high throughput biomolecule detection using transducer arrays. In one embodiment, a transducer array made up of transducer elements may be used to detect byproducts from chemical reactions that involve redox genic tags. Each transducer element may include at least a reaction chamber and a fingerprinting region, configured to flow a fluid from the reaction chamber through the fingerprinting region. The reaction chamber can include a molecule attachment region and the fingerprinting region can include at least one set of electrodes separated by a nanogap for conducting redox cycling reactions. In embodiments, by flowing the chamber content obtained from a reaction of a latent redox tagged probe molecule, a catalyst, and a target molecule in the reaction chamber through the fingerprinting region, the redox cycling reactions can be detected to identify redox-tagged biomolecules.
摘要翻译：各种实施方案提供使用换能器阵列进行高通量生物分子检测的装置，方法和系统。在一个实施例中，由换能器元件组成的换能器阵列可用于检测涉及氧化还原基因标签的化学反应的副产物。每个换能器元件可以至少包括反应室和指纹区域，其被配置为使来自反应室的流体通过指纹区域流动。反应室可以包括分子附着区域，并且指纹区域可以包括由纳米间隙分开的至少一组电极，用于进行氧化还原循环反应。在实施方案中，通过使反应室中潜氧氧化还原标记的探针分子，催化剂和靶分子的反应通过指纹区域流动所述腔室内容物，可以检测氧化还原循环反应以鉴定氧化还原标记的生物分子。

7. 发明授权

US08664008B2 Enzymatic signal generation and detection of binding complexes in stationary fluidic chip 有权
标题翻译：在固定流体芯片中的酶信号产生和结合复合物的检测
公开(公告)号：US08664008B2
公开(公告)日：2014-03-04
申请号：US13543488
申请日：2012-07-06
申请人： David J. Liu , Kai Wu , Xing Su
发明人： David J. Liu , Kai Wu , Xing Su
IPC分类号： G01N33/553 , C12Q1/00
CPC分类号： G01N21/658 , B01L3/50273 , B01L3/502761 , B01L2300/0654 , B01L2400/043 , B01L2400/086 , B82Y15/00 , B82Y30/00 , G01N21/255 , G01N21/6428 , G01N21/645 , G01N21/76 , G01N33/54333 , G01N33/587
摘要： An embodiment of the invention relates to a device for detecting an analyte in a sample. The device comprises a fluidic network and an integrated circuitry component. The fluidic network comprises a sample zone, a cleaning zone and a detection zone. The fluidic network contains a magnetic particle and/or a signal particle. A sample containing an analyte is introduced, and the analyte interacts with the magnetic particle and/or the signal particle through affinity agents. A microcoil array or a mechanically movable permanent magnet is functionally coupled to the fluidic network, which are activatable to generate a magnetic field within a portion of the fluidic network, and move the magnetic particle from the sample zone to the detection zone. A detection element is present which detects optical or electrical signals from the signal particle, thus indicating the presence of the analyte.
摘要翻译：本发明的一个实施例涉及用于检测样品中的分析物的装置。该装置包括流体网络和集成电路部件。流体网络包括样品区，清洁区和检测区。流体网络包含磁性颗粒和/或信号颗粒。引入含有分析物的样品，并且分析物通过亲和试剂与磁性颗粒和/或信号颗粒相互作用。微线圈阵列或机械可移动永磁体在功能上耦合到流体网络，流体网络可激活以在流体网络的一部分内产生磁场，并将磁性颗粒从样品区域移动到检测区域。存在检测元件，其检测来自信号颗粒的光学或电信号，从而指示分析物的存在。

8. 发明授权

US08409877B2 Enzymatic signal generation and detection of binding complexes in stationary fluidic chip 有权
标题翻译：在固定流体芯片中的酶信号产生和结合复合物的检测
公开(公告)号：US08409877B2
公开(公告)日：2013-04-02
申请号：US11647565
申请日：2006-12-29
申请人： David J. Liu , Kai Wu , Xing Su
发明人： David J. Liu , Kai Wu , Xing Su
IPC分类号： G01N33/553 , C12Q1/00
CPC分类号： G01N21/658 , B01L3/50273 , B01L3/502761 , B01L2300/0654 , B01L2400/043 , B01L2400/086 , B82Y15/00 , B82Y30/00 , G01N21/255 , G01N21/6428 , G01N21/645 , G01N21/76 , G01N33/54333 , G01N33/587
摘要： An embodiment of the invention relates to a device for detecting an analyte in a sample. The device comprises a fluidic network and an integrated circuitry component. The fluidic network comprises a sample zone, a cleaning zone and a detection zone. The fluidic network contains a magnetic particle and/or a signal particle. A sample containing an analyte is introduced, and the analyte interacts with the magnetic particle and/or the signal particle through affinity agents. A microcoil array or a mechanically movable permanent magnet is functionally coupled to the fluidic network, which are activatable to generate a magnetic field within a portion of the fluidic network, and move the magnetic particle from the sample zone to the detection zone. A detection element is present which detects optical or electrical signals from the signal particle, thus indicating the presence of the analyte.
摘要翻译：本发明的一个实施例涉及用于检测样品中的分析物的装置。该装置包括流体网络和集成电路部件。流体网络包括样品区，清洁区和检测区。流体网络包含磁性颗粒和/或信号颗粒。引入含有分析物的样品，并且分析物通过亲和试剂与磁性颗粒和/或信号颗粒相互作用。微线圈阵列或机械可移动永磁体在功能上耦合到流体网络，流体网络可激活以在流体网络的一部分内产生磁场，并将磁性颗粒从样品区域移动到检测区域。存在检测元件，其检测来自信号颗粒的光学或电信号，从而指示分析物的存在。

9. 发明申请

US20110159481A1 Solid-phase chelators and electronic biosensors 有权
标题翻译：固相螯合剂和电子生物传感器
公开(公告)号：US20110159481A1
公开(公告)日：2011-06-30
申请号：US12655459
申请日：2009-12-30
申请人： David J. Liu , Xing Su , Kai Wu
发明人： David J. Liu , Xing Su , Kai Wu
IPC分类号： C12Q1/68 , G01N33/50 , G01N27/26
CPC分类号： C12Q1/48 , C12Q1/6837 , C12Q1/6869 , G01N27/4145 , G01N2333/9125 , C12Q2565/607 , C12Q2565/507 , C12Q2565/301
摘要： Methods for sequencing nucleic acids are presented. Sequencing is accomplished through the chemical amplification of the products of DNA synthesis and the detection of the chemically amplified products. In embodiments of the invention, a substrate is provided having a plurality of molecules of DNA to be sequenced attached and a plurality of molecules capable of chelating pyrophosphate ions attached, the DNA molecules to be sequenced are primed, and a next complementary nucleotide is incorporated and excised a plurality of times leading to the buildup of pyrophosphate ions locally around the DNA molecule to be sequenced. Pyrophosphate ions are captured by the substrate-attached chelators and electronically detected to determine the identity of the next complementary nucleic acid in the DNA molecule to be sequenced. Additionally, devices and methods are provided for detecting biomolecules through the detection of pyrophosphate ions.
摘要翻译：介绍了核酸测序方法。通过化学扩增DNA合成产物和化学扩增产物的检测来完成测序。在本发明的实施方案中，提供了具有多个待测序的DNA分子的底物和能够螯合连接的焦磷酸根离子的多个分子，引入待测序的DNA分子，并且掺入下一个互补核苷酸，切除多次导致待测序的DNA分子局部周围的焦磷酸盐离子的积聚。焦磷酸根离子被底物连接的螯合剂捕获并电子检测以确定待测序的DNA分子中下一个互补核酸的同一性。另外，提供了通过检测焦磷酸根离子来检测生物分子的装置和方法。

10. 发明申请

US20080160630A1 Enzymatic signal generation and detection of binding complexes in stationary fluidic chip 有权
标题翻译：在固定流体芯片中的酶信号产生和结合复合物的检测
公开(公告)号：US20080160630A1
公开(公告)日：2008-07-03
申请号：US11647565
申请日：2006-12-29
申请人： David J. Liu , Kai Wu , Xing Su
发明人： David J. Liu , Kai Wu , Xing Su
IPC分类号： G01N21/00 , G01N1/00
CPC分类号： G01N21/658 , B01L3/50273 , B01L3/502761 , B01L2300/0654 , B01L2400/043 , B01L2400/086 , B82Y15/00 , B82Y30/00 , G01N21/255 , G01N21/6428 , G01N21/645 , G01N21/76 , G01N33/54333 , G01N33/587
摘要： An embodiment of the invention relates to a device for detecting an analyte in a sample. The device comprises a fluidic network and an integrated circuitry component. The fluidic network comprises a sample zone, a cleaning zone and a detection zone. The fluidic network contains a magnetic particle and/or a signal particle. A sample containing an analyte is introduced, and the analyte interacts with the magnetic particle and/or the signal particle through affinity agents. A microcoil array or a mechanically movable permanent magnet is functionally coupled to the fluidic network, which are activatable to generate a magnetic field within a portion of the fluidic network, and move the magnetic particle from the sample zone to the detection zone. A detection element is present which detects optical or electrical signals from the signal particle, thus indicating the presence of the analyte.
摘要翻译：本发明的一个实施例涉及用于检测样品中的分析物的装置。该装置包括流体网络和集成电路部件。流体网络包括样品区，清洁区和检测区。流体网络包含磁性粒子和/或信号粒子。引入含有分析物的样品，并且分析物通过亲和试剂与磁性颗粒和/或信号颗粒相互作用。微线圈阵列或机械可移动永磁体在功能上耦合到流体网络，流体网络可激活以在流体网络的一部分内产生磁场，并将磁性颗粒从样品区域移动到检测区域。存在检测元件，其检测来自信号颗粒的光学或电信号，从而指示分析物的存在。

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