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1. 发明申请

US20180238891A1 Cancer Detection Method 审中-公开
公开(公告)号：US20180238891A1
公开(公告)日：2018-08-23
申请号：US15751825
申请日：2016-08-18
申请人： Sienna Cancer Diagnostics Ltd
发明人： Matthew Hoskin , Minesh Lalla , Shannon Turnbull
IPC分类号： G01N33/574
CPC分类号： G01N33/57484 , G01N33/57411 , G01N33/57415 , G01N33/57434 , G01N2333/901 , G01N2333/9125
摘要： The present disclosure relates to methods of determining whether a subject has cancer. More particularly, the present disclosure relates to a method of determining whether a subject has cancer when a pathological assessment of cell morphology is negative for the cancer. More particularly, the present disclosure relates to a method of determining whether a morphologically normal cell is malignant. Identification of malignant cells, when a pathological assessment of cell morphology is negative for cancer, is based upon detecting the binding of an anti-telomerase antibody to clinically relevant cells.

2. 发明申请

US20150198597A1 Method for monitoring PARP activity in cells by PARP activation 审中-公开
标题翻译：通过PARP激活监测细胞PARP活性的方法
公开(公告)号：US20150198597A1
公开(公告)日：2015-07-16
申请号：US14420065
申请日：2013-08-08
申请人： Stichting Het Nederlands Kanker Instituut
发明人： Conchita Vens , Derk Pluim , Marcel Verheij , Johannes Henricus Matthias Schellens
IPC分类号： G01N33/573
CPC分类号： G01N33/573 , C12Q1/48 , G01N2333/91142 , G01N2333/9125 , G01N2400/00
摘要： The current invention provides for improved methods for determining PAR, and uses thereof. These improved methods involve an incubation step at a low temperature, for example around, but above, the freezing point after a PARP activation step. This improves the assay's sensitivity in comparison to current assay's performance. For example, sensitivity can be improved in methods assaying cells obtained from subjects such as human patients. The current invention also allows to provide methods to screen compounds for modulating PARG and/or PARP activity.
摘要翻译：本发明提供了用于确定PAR的改进方法及其用途。这些改进的方法包括在低温下，例如在PARP活化步骤之后但高于冰点的温育步骤。与当前测定的性能相比，这提高了测定的灵敏度。例如，可以在从诸如人类患者的受试者获得的细胞测定方法中提高灵敏度。本发明还允许提供筛选用于调节PARG和/或PARP活性的化合物的方法。

3. 发明授权

US08962279B2 Solid-phase chelators and electronic biosensors 有权
标题翻译：固相螯合剂和电子生物传感器
公开(公告)号：US08962279B2
公开(公告)日：2015-02-24
申请号：US12655459
申请日：2009-12-30
申请人： David J. Liu , Xing Su , Kai Wu
发明人： David J. Liu , Xing Su , Kai Wu
IPC分类号： C12P19/34 , C12M1/00 , C12Q1/48 , C12Q1/68 , G01N27/414
CPC分类号： C12Q1/48 , C12Q1/6837 , C12Q1/6869 , G01N27/4145 , G01N2333/9125 , C12Q2565/607 , C12Q2565/507 , C12Q2565/301
摘要： Methods for sequencing nucleic acids are presented. Sequencing is accomplished through the chemical amplification of the products of DNA synthesis and the detection of the chemically amplified products. In embodiments of the invention, a substrate is provided having a plurality of molecules of DNA to be sequenced attached and a plurality of molecules capable of chelating pyrophosphate ions attached, the DNA molecules to be sequenced are primed, and a next complementary nucleotide is incorporated and excised a plurality of times leading to the buildup of pyrophosphate ions locally around the DNA molecule to be sequenced. Pyrophosphate ions are captured by the substrate-attached chelators and electronically detected to determine the identity of the next complementary nucleic acid in the DNA molecule to be sequenced. Additionally, devices and methods are provided for detecting biomolecules through the detection of pyrophosphate ions.
摘要翻译：介绍了核酸测序方法。通过化学扩增DNA合成产物和化学扩增产物的检测来完成测序。在本发明的实施方案中，提供了具有多个待测序的DNA分子的底物和能够螯合连接的焦磷酸根离子的多个分子，引入待测序的DNA分子，并且掺入下一个互补核苷酸，切除多次导致待测序的DNA分子局部周围的焦磷酸盐离子的积聚。焦磷酸根离子被底物连接的螯合剂捕获并电子检测以确定待测序的DNA分子中下一个互补核酸的同一性。另外，提供了通过检测焦磷酸根离子来检测生物分子的装置和方法。

4. 发明申请

US20110097730A1 Recombinant Sars-Cov nsp12 and the Use of Thereof and the Method for Producing it 有权
标题翻译：重组Sars-Cov nsp12及其用途及其制备方法
公开(公告)号：US20110097730A1
公开(公告)日：2011-04-28
申请号：US12997816
申请日：2008-06-13
申请人： Jong Won Oh , Dae Gyun Ahn
发明人： Jong Won Oh , Dae Gyun Ahn
IPC分类号： C12Q1/68 , C12N9/12 , C12N15/63 , C12N1/21
CPC分类号： C07K14/005 , C12N2770/20022 , G01N2333/165 , G01N2333/9125 , G01N2500/04
摘要： The present invention relates to a recombinant severe acute respiratory syndrome coronavirus (SARS-CoV) non-structural protein (nsp) 12 with an RNA polymerase activity, its expression vector, its preparation method, and its use. According to the present invention, a soluble recombinant SARS-CoV nsp12 with an RdRp activity of initiating SARS-CoV genome synthesis can be over-expressed in the transformed host cells, and conveniently purified with high purity. An in vitro replication system important for studying SARS-CoV replication can be established with the purified recombinant SARS-CoV nsp12. SARS-CoV nsp12 produced by the present invention can also be used as a target for the development of anti-viral agents against SARS-CoV. In addition, materials inhibiting RNA-dependent RNA polymerase (RdRp) activity of nsp12 can be screened efficiently according to the present invention as the optimal conditions for the RdRp assay with SARS-CoV nsp12 were found.
摘要翻译：本发明涉及具有RNA聚合酶活性的重组重症急性呼吸综合征冠状病毒（SARS-CoV）非结构蛋白（nsp）12，其表达载体，其制备方法及其应用。根据本发明，可以在转化的宿主细胞中过表达具有启动SARS-CoV基因组合成的RdRp活性的可溶性重组SARS-CoV nsp12，并以高纯度方便地纯化。可以用纯化的重组SARS-CoV nsp12建立对SARS-CoV复制研究非常重要的体外复制系统。本发明生产的SARS-CoV nsp12也可用作抗SARS-CoV抗病毒剂的开发目标。此外，根据本发明，可以有效地筛选nsp12的RNA依赖性RNA聚合酶（RdRp）活性的材料，因为发现用SARS-CoV nsp12进行RdRp测定的最佳条件。

5. 发明授权

US07879609B2 Regulatory segments of the human gene for telomerase reverse transcriptase 有权
标题翻译：人类基因端粒酶逆转录酶的调节区段
公开(公告)号：US07879609B2
公开(公告)日：2011-02-01
申请号：US11691348
申请日：2007-03-26
申请人： Gregg B. Morin , William H. Andrews
发明人： Gregg B. Morin , William H. Andrews
IPC分类号： C12N15/67 , C12N15/00
CPC分类号： C12N15/113 , A01K2217/05 , A61K38/00 , A61K39/00 , A61K48/00 , C07K16/40 , C07K2319/00 , C12N9/1241 , C12N9/1276 , C12N15/1137 , C12N2310/15 , C12N2310/315 , C12Q1/6886 , C12Q2600/136 , C12Q2600/158 , C12Y207/07049 , G01N33/574 , G01N2333/9125
摘要： The invention provides compositions and methods related to human telomerase reverse transcriptase (hTRT), the catalytic protein subunit of human telomerase. The polynucleotides and polypeptides of the invention are useful for diagnosis, prognosis and treatment of human diseases, for changing the proliferative capacity of cells and organisms, and for identification and screening of compounds and treatments useful for treatment of diseases such as cancers.
摘要翻译：本发明提供了与人类端粒酶逆转录酶（hTRT），人端粒酶的催化蛋白质亚基相关的组合物和方法。本发明的多核苷酸和多肽可用于人类疾病的诊断，预后和治疗，用于改变细胞和生物体的增殖能力，以及用于鉴定和筛选用于治疗诸如癌症的疾病的化合物和治疗。

6. 发明申请

US20100105048A1 FUNCTIONALIZED FLUORESCENT NANOCRYSTAL DETECTION SYSTEM 有权
标题翻译：功能荧光纳米晶体检测系统
公开(公告)号：US20100105048A1
公开(公告)日：2010-04-29
申请号：US12551991
申请日：2009-09-01
申请人： Imad NAASANI
发明人： Imad NAASANI
IPC分类号： C12Q1/68
CPC分类号： G01N33/588 , B82Y15/00 , C12Q1/48 , G01N2333/9125 , Y10S977/774 , Y10S977/902
摘要： The present invention include fluorescent nanocrystals which have high fluorescence intensity, are water soluble, exhibit physical and chemical stability, and whose spectral properties are detectably modified as the size of functional groups bonded to the nanocrystal surface change when contacted with target molecules in a sample. The molecules in the sample add to or reduce the size of functional groups on the fluorescent nanocrystal proportional to the activity and amount of the target molecules. The present invention may be used to detect telomerase in a sample.
摘要翻译：本发明包括具有高荧光强度，水溶性，表现出物理和化学稳定性的荧光纳米晶体，并且当与样品中的靶分子接触时，结合到纳米晶体表面的官能团的尺寸发生变化，其光谱性质可被可检测地修饰。样品中的分子增加或减少荧光纳米晶体上的官能团的大小与靶分子的活性和量成比例。本发明可以用于检测样品中的端粒酶。

7. 发明授权

US07393670B2 Tyrosyl-tRNA synthetase variants 失效
标题翻译：酪氨酰-tRNA合成酶变体
公开(公告)号：US07393670B2
公开(公告)日：2008-07-01
申请号：US10483636
申请日：2002-01-11
申请人： Daisuke Kiga , Kensaku Sakamoto , Ichiro Hirao , Shigeyuki Yokoyama
发明人： Daisuke Kiga , Kensaku Sakamoto , Ichiro Hirao , Shigeyuki Yokoyama
IPC分类号： C12N9/00 , C07K1/00
CPC分类号： C12N9/93 , G01N2333/9125
摘要： Tyrosyl-tRNA synthetases having a modified amino acid sequence whereby unnatural amino acids can be more efficiently incorporated than original natural amino acids. More specifically, tyrosyl-tRNA synthetases having been modified at the amino acid(s) at the 37- and/or 195-positions. A method of modifying tyrosyl-tRNA synthetase characterized in that the position of an amino acid to be modified is determined based on the 3-D structure of a tyrosyl-AMP and of tyrosyl synthetase complex and an amino acid to which this enzyme binds.
摘要翻译：具有修饰氨基酸序列的酪氨酰-tRNA合成酶，由此可以比原始天然氨基酸更有效地掺入非天然氨基酸。更具体地，已经在37位和/或195位的氨基酸修饰的酪氨酰-tRNA合成酶。一种修饰酪氨酰-tRNA合成酶的方法，其特征在于基于酪氨酰-PAMP和酪氨酰合成酶复合物和该酶结合的氨基酸的3-D结构确定待修饰的氨基酸的位置。

8. 发明申请

US20070134707A1 Scintillation proximity assay for measuring polymerase activity 有权
标题翻译：用于测量聚合酶活性的闪烁邻近测定
公开(公告)号：US20070134707A1
公开(公告)日：2007-06-14
申请号：US11635826
申请日：2006-12-08
申请人： Qing-Fen Gan
发明人： Qing-Fen Gan
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/48 , G01N33/54313 , G01N33/60 , G01N2333/91245 , G01N2333/9125
摘要： The present invention provides for methods of assaying the activity of a polymerase enzyme by using a Scintillation Proximity Assay (SPA) without the need of primers modified with affinity tags. The methods of the present invention can be practiced with any number of RNA and DNA polymerase enzymes.
摘要翻译：本发明提供了通过使用闪烁亲和测定（SPA）来测定聚合酶的活性的方法，而不需要用亲和标签修饰的引物。本发明的方法可以用任何数量的RNA和DNA聚合酶来实施。

9. 发明申请

US20070078100A1 Methods of detecting poly(adp-ribose) polymerase and other nad+ utilizing enzymes 审中-公开
标题翻译：检测聚（adp-核糖）聚合酶和其他nad +利用酶的方法
公开(公告)号：US20070078100A1
公开(公告)日：2007-04-05
申请号：US10595360
申请日：2004-10-14
申请人： Paul Hergenrother , Karson Putt
发明人： Paul Hergenrother , Karson Putt
IPC分类号： C12Q1/26 , C07H19/207
CPC分类号： C12Q1/008 , C12Q1/32 , C12Q1/48 , G01N33/573 , G01N2333/9125
摘要： Methods for detecting poly (ADP-ribose) polymerase and other NAD+ utilizing enzymes.
摘要翻译：检测聚（ADP-核糖）聚合酶和其他NAD +利用酶的方法。

10. 发明申请

US20060246479A1 Switch-region: target and method for inhibition of bacterial RNA polymerase 审中-公开
标题翻译：开关区：抑制细菌RNA聚合酶的靶标和方法
公开(公告)号：US20060246479A1
公开(公告)日：2006-11-02
申请号：US11351709
申请日：2006-02-10
申请人： Richard Ebright
发明人： Richard Ebright
IPC分类号： C12Q1/68 , C12N9/22
CPC分类号： G01N33/566 , C07K2299/00 , C12N9/1247 , C12Q1/48 , G01N33/569 , G01N33/573 , G01N2333/9125 , G01N2500/00
摘要： The invention provides a target and methods for specific binding and inhibition of RNA polymerase from bacterial species. The invention provides methods for identifying agents that bind to a bacterial RNA polymerase, and that inhibit an activity of a bacterial RNA polymerase, through interactions with a bacterial RNA polymerase homologous switch-region amino-acid sequence. Said methods comprise preparing a reaction solution comprising the compound to be tested and an entity containing a bacterial RNAP homologous switch-region amino-acid sequence, and detecting binding or inhibition. The invention has applications in control of bacterial gene expression, control of bacterial viability, control of bacterial growth, antibacterial chemistry, and antibacterial therapy.
摘要翻译：本发明提供了从细菌种类特异性结合和抑制RNA聚合酶的靶标和方法。本发明提供了通过与细菌RNA聚合酶同源转换区氨基酸序列的相互作用来鉴定结合细菌RNA聚合酶并且抑制细菌RNA聚合酶活性的试剂的方法。所述方法包括制备包含待测试化合物和含有细菌RNAP同源转换区氨基酸序列的实体的反应溶液，并检测结合或抑制。本发明可用于控制细菌基因表达，控制细菌活力，控制细菌生长，抗菌化学和抗菌治疗。

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