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1. 发明授权

US08383036B2 Methods for neutralizing anthrax or anthrax spores 有权
标题翻译：中和炭疽芽孢或炭疽孢子的方法
公开(公告)号：US08383036B2
公开(公告)日：2013-02-26
申请号：US12973681
申请日：2010-12-20
申请人： Mark A. Sloan , Jeevalatha Vivekananda , Eric A. Holwitt , Johnathan L. Kiel
发明人： Mark A. Sloan , Jeevalatha Vivekananda , Eric A. Holwitt , Johnathan L. Kiel
IPC分类号： A61L2/00
CPC分类号： C12Q1/6825 , C12Q1/6837 , C12Q2565/607
摘要： The present invention concerns methods, compositions and apparatus for neutralizing bioagents, wherein bioagents comprise biowarfare agents, biohazardous agents, biological agents and/or infectious agents. The methods comprise exposing the bioagent to an organic semiconductor and exposing the bioagent and organic semiconductor to a source of energy. Although any source of energy is contemplated, in some embodiments the energy comprises visible light, ultraviolet, infrared, radiofrequency, microwave, laser radiation, pulsed corona discharge or electron beam radiation. Exemplary organic semiconductors include DAT and DALM. In certain embodiments, the organic semiconductor may be attached to one or more binding moieties, such as an antibody, antibody fragment, or nucleic acid ligand. Preferably, the binding moiety has a binding affinity for one or more bioagents to be neutralized. Other embodiments concern an apparatus comprising an organic semiconductor and an energy source. In preferred embodiments, the methods, compositions and apparatus are used for neutralizing anthrax spores.
摘要翻译：本发明涉及用于中和生物标签的方法，组合物和装置，其中生物制剂包括生物武器，生物危害剂，生物制剂和/或感染剂。所述方法包括将生物制剂暴露于有机半导体并将生物制剂和有机半导体暴露于能量源。尽管预期了任何能量源，但在一些实施例中，能量包括可见光，紫外线，红外线，射频，微波，激光辐射，脉冲电晕放电或电子束辐射。示例性有机半导体包括DAT和DALM。在某些实施方案中，有机半导体可以连接到一个或多个结合部分，例如抗体，抗体片段或核酸配体。优选地，结合部分对一种或多种待中和的生物剂具有结合亲和力。其他实施例涉及包括有机半导体和能量源的装置。在优选的实施方案中，所述方法，组合物和装置用于中和炭疽芽孢。

2. 发明授权

US07892484B2 Methods and compositions for neutralizing anthrax and other bioagents 有权
标题翻译：用于中和炭疽和其他生物制剂的方法和组合物
公开(公告)号：US07892484B2
公开(公告)日：2011-02-22
申请号：US10291336
申请日：2002-11-08
申请人： Mark A. Sloan , Jeevalatha Vivekananda , Eric A. Holwitt , Johnathan L. Kiel
发明人： Mark A. Sloan , Jeevalatha Vivekananda , Eric A. Holwitt , Johnathan L. Kiel
IPC分类号： A61L2/00
CPC分类号： C12Q1/6825 , C12Q1/6837 , C12Q2565/607
摘要： The present invention concerns methods, compositions and apparatus for neutralizing bioagents, wherein bioagents comprise biowarfare agents, biohazardous agents, biological agents and/or infectious agents. The methods comprise exposing the bioagent to an organic semiconductor and exposing the bioagent and organic semiconductor to a source of energy. Although any source of energy is contemplated, in some embodiments the energy comprises visible light, ultraviolet, infrared, radiofrequency, microwave, laser radiation, pulsed corona discharge or electron beam radiation. Exemplary organic semiconductors include DAT and DALM. In certain embodiments, the organic semiconductor may be attached to one or more binding moieties, such as an antibody, antibody fragment, or nucleic acid ligand. Preferably, the binding moiety has a binding affinity for one or more bioagents to be neutralized. Other embodiments concern an apparatus comprising an organic semiconductor and an energy source. In preferred embodiments, the methods, compositions and apparatus are used for neutralizing anthrax spores.
摘要翻译：本发明涉及用于中和生物标签的方法，组合物和装置，其中生物制剂包括生物武器，生物危害剂，生物制剂和/或感染剂。所述方法包括将生物制剂暴露于有机半导体并将生物制剂和有机半导体暴露于能量源。尽管预期了任何能量源，但在一些实施例中，能量包括可见光，紫外线，红外线，射频，微波，激光辐射，脉冲电晕放电或电子束辐射。示例性有机半导体包括DAT和DALM。在某些实施方案中，有机半导体可以连接到一个或多个结合部分，例如抗体，抗体片段或核酸配体。优选地，结合部分对一种或多种待中和的生物剂具有结合亲和力。其他实施例涉及包括有机半导体和能量源的装置。在优选的实施方案中，所述方法，组合物和装置用于中和炭疽芽孢。

3. 发明申请

US20120134877A1 METHODS FOR NEUTRALIZING ANTHRAX OR ANTHRAX SPORES 有权
标题翻译：用于中和ANTHRAX或ANTHRAX SPORES的方法
公开(公告)号：US20120134877A1
公开(公告)日：2012-05-31
申请号：US12973681
申请日：2010-12-20
申请人： Mark A. SLOAN , Jeevalatha Vivekananda , Eric A. Holwitt , Johnathan L. Kiel
发明人： Mark A. SLOAN , Jeevalatha Vivekananda , Eric A. Holwitt , Johnathan L. Kiel
IPC分类号： A61L2/12 , A61L2/14 , A61L2/08
CPC分类号： C12Q1/6825 , C12Q1/6837 , C12Q2565/607
摘要： The present invention concerns methods, compositions and apparatus for neutralizing bioagents, wherein bioagents comprise biowarfare agents, biohazardous agents, biological agents and/or infectious agents. The methods comprise exposing the bioagent to an organic semiconductor and exposing the bioagent and organic semiconductor to a source of energy. Although any source of energy is contemplated, in some embodiments the energy comprises visible light, ultraviolet, infrared, radiofrequency, microwave, laser radiation, pulsed corona discharge or electron beam radiation. Exemplary organic semiconductors include DAT and DALM. In certain embodiments, the organic semiconductor may be attached to one or more binding moieties, such as an antibody, antibody fragment, or nucleic acid ligand. Preferably, the binding moiety has a binding affinity for one or more bioagents to be neutralized. Other embodiments concern an apparatus comprising an organic semiconductor and an energy source. In preferred embodiments, the methods, compositions and apparatus are used for neutralizing anthrax spores.
摘要翻译：本发明涉及用于中和生物标签的方法，组合物和装置，其中生物制剂包括生物武器，生物危害剂，生物制剂和/或感染剂。所述方法包括将生物制剂暴露于有机半导体并将生物制剂和有机半导体暴露于能量源。尽管预期了任何能量源，但在一些实施例中，能量包括可见光，紫外线，红外线，射频，微波，激光辐射，脉冲电晕放电或电子束辐射。示例性有机半导体包括DAT和DALM。在某些实施方案中，有机半导体可以连接到一个或多个结合部分，例如抗体，抗体片段或核酸配体。优选地，结合部分对一种或多种待中和的生物剂具有结合亲和力。其他实施例涉及包括有机半导体和能量源的装置。在优选的实施方案中，所述方法，组合物和装置用于中和炭疽芽孢。

4. 发明授权

US06569630B1 Methods and compositions for aptamers against anthrax 有权
标题翻译：针对炭疽的适体的方法和组成
公开(公告)号：US06569630B1
公开(公告)日：2003-05-27
申请号：US09978753
申请日：2001-10-15
申请人： Jeevalatha Vivekananda , Johnathan L. Kiel
发明人： Jeevalatha Vivekananda , Johnathan L. Kiel
IPC分类号： C12Q168
CPC分类号： C12Q1/6825 , C12Q1/6837 , C12Q2565/607
摘要： The present invention concerns methods of preparing nucleic acid ligands against anthrax spores, compositions comprising anthrax specific nucleic acid ligands and methods of use of such ligands for detection and/or neutralization of anthrax spores.
摘要翻译：本发明涉及制备针对炭疽孢子的核酸配体的方法，包含炭疽特异性核酸配体的组合物和用于检测和/或中和炭疽孢子的这种配体的使用方法。

5. 发明授权

US08318438B2 Aptamer-based assays 有权
公开(公告)号：US08318438B2
公开(公告)日：2012-11-27
申请号：US12072758
申请日：2008-02-27
申请人： Jeevalatha Vivekananda , Johnathan L. Kiel
发明人： Jeevalatha Vivekananda , Johnathan L. Kiel
IPC分类号： C12Q1/00 , C12Q1/68 , G01N33/00 , G01N33/554 , G01N33/569
CPC分类号： G01N33/5308 , G01N2333/195
摘要： We describe examples using aptamers for capturing and reporting the presence of a target, such as a pathogen. Examples described here include a set of aptamers that are specific to F. tularensisis. Other examples described here include an Aptamer-Linked Immobilized Sorbent Assay (ALISA) and dot blot assay. An example of a method provided here comprises: providing a set of DNA sequences that exhibit high binding affinity to target antigen, placing the DNA sequences in a sandwich aptamer-linked immobilized sorbent assay (ALISA), contacting the DNA sequences with a sample, and detecting whether the target is present in the sample. Some alternative implementations may include dot blots and different reporters. Quantum dot sandwich assays and quantum dot de-quenching reporters can be used.

6. 发明申请

US20120157670A1 Aptamer-based assays 有权
标题翻译：基于Aptamer的测定
公开(公告)号：US20120157670A1
公开(公告)日：2012-06-21
申请号：US12072758
申请日：2008-02-27
申请人： Jeevalatha Vivekananda , Johnathan L. Kiel
发明人： Jeevalatha Vivekananda , Johnathan L. Kiel
IPC分类号： C07H21/04 , G01N33/569 , G01N33/53
CPC分类号： G01N33/5308 , G01N2333/195
摘要： We describe examples using aptamers for capturing and reporting the presence of a target, such as a pathogen. Examples described here include a set of aptamers that are specific to F. tularensisis. Other examples described here include an Aptamer-Linked Immobilized Sorbent Assay (ALISA) and dot blot assay. An example of a method provided here comprises: providing a set of DNA sequences that exhibit high binding affinity to target antigen, placing the DNA sequences in a sandwich aptamer-linked immobilized sorbent assay (ALISA), contacting the DNA sequences with a sample, and detecting whether the target is present in the sample. Some alternative implementations may include dot blots and different reporters. Quantum dot sandwich assays and quantum dot de-quenching reporters can be used.
摘要翻译：我们描述使用适配体捕获和报告目标（例如病原体）的存在的实例。这里描述的实例包括一组特异于土拉氏木霉的适体。本文描述的其它实例包括Aptamer-Linked固定吸附测定（ALISA）和斑点印迹测定。本文提供的方法的实例包括：提供一组对靶抗原具有高结合亲和力的DNA序列，将DNA序列置于夹心适体连接的固定化吸附剂测定（ALISA）中，使DNA序列与样品接触，以及检测目标是否存在于样本中。一些替代实现可以包括点印迹和不同的记者。可以使用量子点夹心测定法和量子点去猝灭记录仪。

7. 发明授权

US5028541A Flow-through cell cultivation system 失效
标题翻译：流通细胞培养系统
公开(公告)号：US5028541A
公开(公告)日：1991-07-02
申请号：US56034
申请日：1987-06-01
申请人： Johnathan L. Kiel , David N. Erwin , David M. Simmons
发明人： Johnathan L. Kiel , David N. Erwin , David M. Simmons
IPC分类号： C12M3/00
CPC分类号： C12M35/02 , C12M23/42 , C12M29/10 , C12M41/12
摘要： A flow-through cell cultivation apparatus and method is described. The flow-through cell cultivation system comprises a flowcell, a chamber inside the flowcell for holding cells, and a flowcell holder. The flowcell has a lower intake port for flowing liquid nutrient through a cellbed inside the flowcell and an upper outlet port for flowing the liquid nutrient out of the flowcell. The flowcell also includes a chuck for receipt of a thermal probe. The probe is made of electromagnetically non-interactive material. The flowcell is enclosed inside the flowcell holder. The flowcell holder includes a pair of intake ports into a cavity having an open end at the flowcell for turbulently flowing a temperature controlled gas against the flowcell. The cavity has rough walls to promote the turbulent flow. The flowcell holder includes an exhaust port for flowing the gas out of the flowcell holder which also serves as a port for another thermal probe. A one-way valve prevents flow of the liquid nutrient out of the chamber through the intake port. Temperature controlled air is supplied to the flowcell holder by dividing air from an air supply into two conduits. One conduit travels through a coil inside a refrigerated liquid bath and the other through a coil inside a heated liquid bath. The heated and cooled air are recombined to make an even temperature air supply which is then divided once more to provide the two air supplies for creating a turbulent supply of temperature controlled air through the two flowcell holder intake ports. The flowcell may include on one side a semi-permeable membrane for introducing a gas/liquid interface to the cellbed.
摘要翻译：描述了流通细胞培养装置和方法。流通池培养系统包括流通池，用于保持电池的流通池内的室和流通池保持器。流通池具有用于使液体营养物流过流动池内的池底的较低进气口和用于使液体营养物流出流动池的上部出口。流通池还包括用于接收热探针的卡盘。探头由电磁非交互材料制成。流通池封闭在流通池支架内。流通池保持器包括一对进入端口，其具有在流通池处具有开口端的空腔，用于将受温度控制的气体湍流地流向流动池。空腔具有粗糙的壁，以促进湍流。流通池保持器包括用于将气体从流动池保持器流出的排气口，其还用作另一热探针的端口。单向阀防止液体营养物质通过进气口流出室外。通过将空气从空气供应分成两个管道，将温度控制的空气供应到流通池保持器。一个导管穿过冷藏液槽内的线圈，另一个通过加热液槽内的线圈。加热和冷却的空气被重新组合以产生均匀的温度空气供应，然后再分配一次，以提供两个空气供应，以通过两个流通池保持器进气口产生温度受控空气的湍流供应。流动池可以在一侧包括用于将气体/液体界面引入细胞床的半透膜。

8. 发明授权

US09273345B2 Methods and compositions for processes of rapid selection and production of nucleic acid aptamers 有权
标题翻译：快速选择和生产核酸适体的方法和组合物
公开(公告)号：US09273345B2
公开(公告)日：2016-03-01
申请号：US11965039
申请日：2007-12-27
申请人： Johnathan L. Kiel , Eric A. Holwitt , Michael (Maomian) Fan , Shelly D. Roper
发明人： Johnathan L. Kiel , Eric A. Holwitt , Michael (Maomian) Fan , Shelly D. Roper
IPC分类号： C12N15/115 , C12Q1/68
CPC分类号： C12Q1/6811 , C12N15/115 , Y10T436/143333 , C12Q2525/205 , C12Q2565/101
摘要： Embodiments herein relate to compositions and methods for making and using aptamers, for example, DNA aptamers (DCEs) and/or RNA aptamers. In some embodiments, methods relate to making and amplifying target DCEs. In certain embodiments, methods for making capture elements or aptamers concern using a reporter moiety and signal reducing moiety prior to amplifying a target-specific capture element. In some embodiments, methods disclosed herein may be used to rapidly generate large quantities of aptamers such as DCEs directed to a particular target agent. Some embodiments relate to systems for performing automated generation of aptamers.
摘要翻译：本文的实施方案涉及用于制备和使用适体的组合物和方法，例如DNA适体（DCE）和/或RNA适体。在一些实施方案中，方法涉及制备和扩增目标DCE。在某些实施方案中，用于制备捕获元件或适体的方法在放大靶特异性捕获元件之前关注使用报道部分和信号降低部分。在一些实施方案中，本文公开的方法可用于快速产生大量适配体，例如针对特定目标试剂的DCE。一些实施例涉及用于执行适配体的自动生成的系统。

9. 发明授权

US08092988B1 Medium and method of deriving and using a mutated bacteria strain 有权
标题翻译：培养基和衍生和使用突变菌株的方法
公开(公告)号：US08092988B1
公开(公告)日：2012-01-10
申请号：US11820039
申请日：2007-05-25
申请人： Jill E. Parker , Johnathan L. Kiel , Homer Gifford , John L. Alls , Pedro J. Morales, legal representative
发明人： Jill E. Parker , Johnathan L. Kiel , Homer Gifford , John L. Alls
IPC分类号： C12Q1/00 , C12N15/00
CPC分类号： C12R1/07 , A61K39/07 , A61K2039/522
摘要： A new strain of Bacillus anthracis derived from the Sterne vaccine strain of Bacillus anthracis by growth on a high-nitrate-concentration, 3-amino-L-tyrosine growth medium.
摘要翻译：通过在高硝酸盐浓度的3-氨基-L-酪氨酸生长培养基上生长，衍生自炭疽芽孢杆菌的Sterne疫苗株的新型芽孢杆菌菌株。

10. 发明授权

US5856108A Biosynthesis of diazomelanin and diazoluminomelanin and methods thereof 失效
标题翻译：重氮木兰素和二叠氮木兰素的生物合成及其方法
公开(公告)号：US5856108A
公开(公告)日：1999-01-05
申请号：US779694
申请日：1991-10-21
申请人： Johnathan L. Kiel , Jill E. Parker , Eric A. Holwitt , Harvey A. Schwertner
发明人： Johnathan L. Kiel , Jill E. Parker , Eric A. Holwitt , Harvey A. Schwertner
IPC分类号： C12N9/02 , C12P17/12 , C12Q1/12 , G01N33/58 , C12Q1/00 , C12Q1/04 , C12Q1/26
CPC分类号： C12N9/0036 , C12P17/12 , C12Q1/12 , G01N33/582
摘要： There is provided a method for producing diazoluminomelanin (DALM) which comprises culturing in a medium containing nitrate, 3-amino-L-tyrosine (3-AT) and luminol under suitable metabolic conditions, a microorganism containing nitrate reductase.Also provided is a method for directly detecting microorganisms containing nitrate reductase or those into which nitrate reductase can be introduced by recombinant DNA technology, which comprises culturing the microorganism in a medium containing nitrate, 3-amino-L-tyrosine (3-AT) and luminol under suitable metabolic conditions, transferring the medium to a microtiter plate or tube coated with antibody or an antiligand to which the microorganism would specifically bind, washing the plate or tube and activating luminescence.Further, there is provided a method for producing diazomelanin (DM) which comprises culturing in a medium containing nitrate and 3-amino-L-tyrosine (3-AT) under suitable metabolic conditions, a microorganism containing nitrate reductase.Yet further, there is provided a method for directly detecting microorganisms containing nitrate reductase or those into which nitrate reductase can be introduced by recombinant DNA technology, which comprises culturing the microorganism in a medium containing nitrate and 3-amino-L-tyrosine (3-AT) under suitable metabolic conditions, transferring the medium to a microtiter plate or tube coated with antibody or an antiligand to which the microorganism would specifically bind, washing the plate or tube, adding luminol and activating luminescence.
摘要翻译：提供了一种生产重氮酚蓝蛋白（DALM）的方法，该方法包括在合适的代谢条件下，在含硝酸盐，3-氨基-L-酪氨酸（3-AT）和鲁米诺的培养基中培养含有硝酸还原酶的微生物。还提供了一种用于直接检测含有硝酸还原酶的微生物的方法或其中可以通过重组DNA技术引入硝酸还原酶的方法，其包括在含有硝酸盐，3-氨基-L-酪氨酸（3-AT）和将鲁米诺转移到适当的代谢条件下，将培养基转移到用抗体或微生物将特异性结合的抗配体的微量滴定板或管，洗涤板或管并激活发光。此外，提供了一种生产重氮木兰素（DM）的方法，其包括在适当的代谢条件下在含有硝酸盐和3-氨基-L-酪氨酸（3-AT）的培养基中培养含有硝酸还原酶的微生物。此外，提供了一种直接检测含有硝酸还原酶的微生物的方法或其中可以通过重组DNA技术引入硝酸还原酶的方法，其包括在含有硝酸盐和3-氨基-L-酪氨酸（3- AT），将培养基转移到用抗体或微生物将特异性结合的抗配体的微量滴定板或管，洗涤板或管，加入鲁米诺并激活发光。

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