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1. 发明授权

US08383036B2 Methods for neutralizing anthrax or anthrax spores 有权
标题翻译：中和炭疽芽孢或炭疽孢子的方法
公开(公告)号：US08383036B2
公开(公告)日：2013-02-26
申请号：US12973681
申请日：2010-12-20
申请人： Mark A. Sloan , Jeevalatha Vivekananda , Eric A. Holwitt , Johnathan L. Kiel
发明人： Mark A. Sloan , Jeevalatha Vivekananda , Eric A. Holwitt , Johnathan L. Kiel
IPC分类号： A61L2/00
CPC分类号： C12Q1/6825 , C12Q1/6837 , C12Q2565/607
摘要： The present invention concerns methods, compositions and apparatus for neutralizing bioagents, wherein bioagents comprise biowarfare agents, biohazardous agents, biological agents and/or infectious agents. The methods comprise exposing the bioagent to an organic semiconductor and exposing the bioagent and organic semiconductor to a source of energy. Although any source of energy is contemplated, in some embodiments the energy comprises visible light, ultraviolet, infrared, radiofrequency, microwave, laser radiation, pulsed corona discharge or electron beam radiation. Exemplary organic semiconductors include DAT and DALM. In certain embodiments, the organic semiconductor may be attached to one or more binding moieties, such as an antibody, antibody fragment, or nucleic acid ligand. Preferably, the binding moiety has a binding affinity for one or more bioagents to be neutralized. Other embodiments concern an apparatus comprising an organic semiconductor and an energy source. In preferred embodiments, the methods, compositions and apparatus are used for neutralizing anthrax spores.
摘要翻译：本发明涉及用于中和生物标签的方法，组合物和装置，其中生物制剂包括生物武器，生物危害剂，生物制剂和/或感染剂。所述方法包括将生物制剂暴露于有机半导体并将生物制剂和有机半导体暴露于能量源。尽管预期了任何能量源，但在一些实施例中，能量包括可见光，紫外线，红外线，射频，微波，激光辐射，脉冲电晕放电或电子束辐射。示例性有机半导体包括DAT和DALM。在某些实施方案中，有机半导体可以连接到一个或多个结合部分，例如抗体，抗体片段或核酸配体。优选地，结合部分对一种或多种待中和的生物剂具有结合亲和力。其他实施例涉及包括有机半导体和能量源的装置。在优选的实施方案中，所述方法，组合物和装置用于中和炭疽芽孢。

2. 发明授权

US07892484B2 Methods and compositions for neutralizing anthrax and other bioagents 有权
标题翻译：用于中和炭疽和其他生物制剂的方法和组合物
公开(公告)号：US07892484B2
公开(公告)日：2011-02-22
申请号：US10291336
申请日：2002-11-08
申请人： Mark A. Sloan , Jeevalatha Vivekananda , Eric A. Holwitt , Johnathan L. Kiel
发明人： Mark A. Sloan , Jeevalatha Vivekananda , Eric A. Holwitt , Johnathan L. Kiel
IPC分类号： A61L2/00
CPC分类号： C12Q1/6825 , C12Q1/6837 , C12Q2565/607
摘要： The present invention concerns methods, compositions and apparatus for neutralizing bioagents, wherein bioagents comprise biowarfare agents, biohazardous agents, biological agents and/or infectious agents. The methods comprise exposing the bioagent to an organic semiconductor and exposing the bioagent and organic semiconductor to a source of energy. Although any source of energy is contemplated, in some embodiments the energy comprises visible light, ultraviolet, infrared, radiofrequency, microwave, laser radiation, pulsed corona discharge or electron beam radiation. Exemplary organic semiconductors include DAT and DALM. In certain embodiments, the organic semiconductor may be attached to one or more binding moieties, such as an antibody, antibody fragment, or nucleic acid ligand. Preferably, the binding moiety has a binding affinity for one or more bioagents to be neutralized. Other embodiments concern an apparatus comprising an organic semiconductor and an energy source. In preferred embodiments, the methods, compositions and apparatus are used for neutralizing anthrax spores.
摘要翻译：本发明涉及用于中和生物标签的方法，组合物和装置，其中生物制剂包括生物武器，生物危害剂，生物制剂和/或感染剂。所述方法包括将生物制剂暴露于有机半导体并将生物制剂和有机半导体暴露于能量源。尽管预期了任何能量源，但在一些实施例中，能量包括可见光，紫外线，红外线，射频，微波，激光辐射，脉冲电晕放电或电子束辐射。示例性有机半导体包括DAT和DALM。在某些实施方案中，有机半导体可以连接到一个或多个结合部分，例如抗体，抗体片段或核酸配体。优选地，结合部分对一种或多种待中和的生物剂具有结合亲和力。其他实施例涉及包括有机半导体和能量源的装置。在优选的实施方案中，所述方法，组合物和装置用于中和炭疽芽孢。

3. 发明申请

US20120134877A1 METHODS FOR NEUTRALIZING ANTHRAX OR ANTHRAX SPORES 有权
标题翻译：用于中和ANTHRAX或ANTHRAX SPORES的方法
公开(公告)号：US20120134877A1
公开(公告)日：2012-05-31
申请号：US12973681
申请日：2010-12-20
申请人： Mark A. SLOAN , Jeevalatha Vivekananda , Eric A. Holwitt , Johnathan L. Kiel
发明人： Mark A. SLOAN , Jeevalatha Vivekananda , Eric A. Holwitt , Johnathan L. Kiel
IPC分类号： A61L2/12 , A61L2/14 , A61L2/08
CPC分类号： C12Q1/6825 , C12Q1/6837 , C12Q2565/607
摘要： The present invention concerns methods, compositions and apparatus for neutralizing bioagents, wherein bioagents comprise biowarfare agents, biohazardous agents, biological agents and/or infectious agents. The methods comprise exposing the bioagent to an organic semiconductor and exposing the bioagent and organic semiconductor to a source of energy. Although any source of energy is contemplated, in some embodiments the energy comprises visible light, ultraviolet, infrared, radiofrequency, microwave, laser radiation, pulsed corona discharge or electron beam radiation. Exemplary organic semiconductors include DAT and DALM. In certain embodiments, the organic semiconductor may be attached to one or more binding moieties, such as an antibody, antibody fragment, or nucleic acid ligand. Preferably, the binding moiety has a binding affinity for one or more bioagents to be neutralized. Other embodiments concern an apparatus comprising an organic semiconductor and an energy source. In preferred embodiments, the methods, compositions and apparatus are used for neutralizing anthrax spores.
摘要翻译：本发明涉及用于中和生物标签的方法，组合物和装置，其中生物制剂包括生物武器，生物危害剂，生物制剂和/或感染剂。所述方法包括将生物制剂暴露于有机半导体并将生物制剂和有机半导体暴露于能量源。尽管预期了任何能量源，但在一些实施例中，能量包括可见光，紫外线，红外线，射频，微波，激光辐射，脉冲电晕放电或电子束辐射。示例性有机半导体包括DAT和DALM。在某些实施方案中，有机半导体可以连接到一个或多个结合部分，例如抗体，抗体片段或核酸配体。优选地，结合部分对一种或多种待中和的生物剂具有结合亲和力。其他实施例涉及包括有机半导体和能量源的装置。在优选的实施方案中，所述方法，组合物和装置用于中和炭疽芽孢。

4. 发明授权

US08628955B2 Compositions, methods and uses for biosynthetic plasmid integrated capture elements 有权
标题翻译：生物合成质粒综合捕获元素的组成，方法和用途
公开(公告)号：US08628955B2
公开(公告)日：2014-01-14
申请号：US12792492
申请日：2010-06-02
申请人： Johnathan L. Kiel , Amanda Tijerina , Eric A. Holwitt , Jill Parker , Mark A. Sloan , Melanie Woitaske , Maomian Fan
发明人： Johnathan L. Kiel , Amanda Tijerina , Eric A. Holwitt , Jill Parker , Mark A. Sloan , Melanie Woitaske , Maomian Fan
IPC分类号： C12N15/63 , C07H21/04
CPC分类号： B82Y5/00 , C12N15/111 , C12N2320/32 , C12N2330/00 , C12P19/34
摘要： Embodiments herein report compositions, systems and methods for making and using plasmid vectors and nanotube complexes. In certain embodiments, compositions, systems and methods herein include making plasmid vectors having aptamer inserts. In some embodiments, methods disclosed herein may be used to rapidly generate large quantities of plasmid vectors having aptamer inserts directed to a particular target agent. Other aspects concern plasmid constructs associated with organic semiconductors. Yet other aspects concern complexes of nanotubes associated with dsDNA aptamers and tracking molecules.
摘要翻译：本文的实施方案报道了用于制备和使用质粒载体和纳米管复合物的组合物，系统和方法。在某些实施方案中，本文的组合物，系统和方法包括制备具有适体插入片段的质粒载体。在一些实施方案中，本文公开的方法可用于快速产生具有针对特定目标试剂的适配子插入物的大量质粒载体。其他方面涉及与有机半导体相关的质粒构建体。其他方面涉及与dsDNA适体和跟踪分子相关的纳米管的复合物。

5. 发明授权

US5658673A Microwave-sensitive article 失效
标题翻译：微波敏感文章
公开(公告)号：US5658673A
公开(公告)日：1997-08-19
申请号：US504306
申请日：1995-07-20
申请人： Eric A. Holwitt , Johnathan L. Kiel , David N. Erwin
发明人： Eric A. Holwitt , Johnathan L. Kiel , David N. Erwin
IPC分类号： C03C17/34 , G01R29/08 , B32B27/08 , B32B27/34 , B32B27/38 , B32B27/40
CPC分类号： C03C17/3405 , G01R29/0857 , C03C2217/42 , C03C2217/475 , G01R29/0878 , Y10T428/31511 , Y10T428/31515 , Y10T428/31522 , Y10T428/31525 , Y10T428/31529 , Y10T428/31551 , Y10T428/31591 , Y10T428/31605 , Y10T428/31609 , Y10T428/31725 , Y10T428/31768 , Y10T428/31772 , Y10T428/31775 , Y10T428/31779 , Y10T428/31783 , Y10T428/31844 , Y10T428/31848
摘要： An article which is sensitive to non-ionizing electromagnetic radiation in the radiofrequency radiation range, consisting essentially of a substrate, a layer of a binder material and a layer of activated diazoluminomelanin. This article is prepared by coating at least one surface of a substrate with a binder, immersing the binder-coated substrate into a solution comprising luminol, 3AT and a soluble nitrite for a period of about 2 to 12 days to provide a layer of diazoluminomelanin (DALM) on the binder layer, removing the DALM-coated film from the solution and rinsing the same, activating the DALM with sodium bicarbonate and hydrogen peroxide for a period of about 2 to 12 days.
摘要翻译：在射频辐射范围内对非电离电磁辐射敏感的物品，其基本上由基底，粘合剂材料层和活化的二叠氮木兰素层组成。本文通过用粘合剂涂覆基材的至少一个表面来制备，将粘合剂涂覆的基材浸入包含鲁米诺3AT和可溶性亚硝酸盐的溶液中约2至12天的时间以提供一层二叠氮木兰宁（ DALM），从溶液中除去DALM涂膜并漂洗，用碳酸氢钠和过氧化氢活化DALM约2-12天。

6. 发明授权

US09273345B2 Methods and compositions for processes of rapid selection and production of nucleic acid aptamers 有权
标题翻译：快速选择和生产核酸适体的方法和组合物
公开(公告)号：US09273345B2
公开(公告)日：2016-03-01
申请号：US11965039
申请日：2007-12-27
申请人： Johnathan L. Kiel , Eric A. Holwitt , Michael (Maomian) Fan , Shelly D. Roper
发明人： Johnathan L. Kiel , Eric A. Holwitt , Michael (Maomian) Fan , Shelly D. Roper
IPC分类号： C12N15/115 , C12Q1/68
CPC分类号： C12Q1/6811 , C12N15/115 , Y10T436/143333 , C12Q2525/205 , C12Q2565/101
摘要： Embodiments herein relate to compositions and methods for making and using aptamers, for example, DNA aptamers (DCEs) and/or RNA aptamers. In some embodiments, methods relate to making and amplifying target DCEs. In certain embodiments, methods for making capture elements or aptamers concern using a reporter moiety and signal reducing moiety prior to amplifying a target-specific capture element. In some embodiments, methods disclosed herein may be used to rapidly generate large quantities of aptamers such as DCEs directed to a particular target agent. Some embodiments relate to systems for performing automated generation of aptamers.
摘要翻译：本文的实施方案涉及用于制备和使用适体的组合物和方法，例如DNA适体（DCE）和/或RNA适体。在一些实施方案中，方法涉及制备和扩增目标DCE。在某些实施方案中，用于制备捕获元件或适体的方法在放大靶特异性捕获元件之前关注使用报道部分和信号降低部分。在一些实施方案中，本文公开的方法可用于快速产生大量适配体，例如针对特定目标试剂的DCE。一些实施例涉及用于执行适配体的自动生成的系统。

7. 发明授权

US5856108A Biosynthesis of diazomelanin and diazoluminomelanin and methods thereof 失效
标题翻译：重氮木兰素和二叠氮木兰素的生物合成及其方法
公开(公告)号：US5856108A
公开(公告)日：1999-01-05
申请号：US779694
申请日：1991-10-21
申请人： Johnathan L. Kiel , Jill E. Parker , Eric A. Holwitt , Harvey A. Schwertner
发明人： Johnathan L. Kiel , Jill E. Parker , Eric A. Holwitt , Harvey A. Schwertner
IPC分类号： C12N9/02 , C12P17/12 , C12Q1/12 , G01N33/58 , C12Q1/00 , C12Q1/04 , C12Q1/26
CPC分类号： C12N9/0036 , C12P17/12 , C12Q1/12 , G01N33/582
摘要： There is provided a method for producing diazoluminomelanin (DALM) which comprises culturing in a medium containing nitrate, 3-amino-L-tyrosine (3-AT) and luminol under suitable metabolic conditions, a microorganism containing nitrate reductase.Also provided is a method for directly detecting microorganisms containing nitrate reductase or those into which nitrate reductase can be introduced by recombinant DNA technology, which comprises culturing the microorganism in a medium containing nitrate, 3-amino-L-tyrosine (3-AT) and luminol under suitable metabolic conditions, transferring the medium to a microtiter plate or tube coated with antibody or an antiligand to which the microorganism would specifically bind, washing the plate or tube and activating luminescence.Further, there is provided a method for producing diazomelanin (DM) which comprises culturing in a medium containing nitrate and 3-amino-L-tyrosine (3-AT) under suitable metabolic conditions, a microorganism containing nitrate reductase.Yet further, there is provided a method for directly detecting microorganisms containing nitrate reductase or those into which nitrate reductase can be introduced by recombinant DNA technology, which comprises culturing the microorganism in a medium containing nitrate and 3-amino-L-tyrosine (3-AT) under suitable metabolic conditions, transferring the medium to a microtiter plate or tube coated with antibody or an antiligand to which the microorganism would specifically bind, washing the plate or tube, adding luminol and activating luminescence.
摘要翻译：提供了一种生产重氮酚蓝蛋白（DALM）的方法，该方法包括在合适的代谢条件下，在含硝酸盐，3-氨基-L-酪氨酸（3-AT）和鲁米诺的培养基中培养含有硝酸还原酶的微生物。还提供了一种用于直接检测含有硝酸还原酶的微生物的方法或其中可以通过重组DNA技术引入硝酸还原酶的方法，其包括在含有硝酸盐，3-氨基-L-酪氨酸（3-AT）和将鲁米诺转移到适当的代谢条件下，将培养基转移到用抗体或微生物将特异性结合的抗配体的微量滴定板或管，洗涤板或管并激活发光。此外，提供了一种生产重氮木兰素（DM）的方法，其包括在适当的代谢条件下在含有硝酸盐和3-氨基-L-酪氨酸（3-AT）的培养基中培养含有硝酸还原酶的微生物。此外，提供了一种直接检测含有硝酸还原酶的微生物的方法或其中可以通过重组DNA技术引入硝酸还原酶的方法，其包括在含有硝酸盐和3-氨基-L-酪氨酸（3- AT），将培养基转移到用抗体或微生物将特异性结合的抗配体的微量滴定板或管，洗涤板或管，加入鲁米诺并激活发光。

8. 发明申请

US20090004644A1 METHODS AND COMPOSITIONS FOR PROCESSES OF RAPID SELECTION AND PRODUCTION OF NUCLEIC ACID APTAMERS 有权
标题翻译：快速选择和生产核酸APTAMERS的方法和组合物
公开(公告)号：US20090004644A1
公开(公告)日：2009-01-01
申请号：US11965039
申请日：2007-12-27
申请人： Johnathan L. Kiel , Eric A. Holwitt , Michael (Maomian) Fan , Shelly D. Roper
发明人： Johnathan L. Kiel , Eric A. Holwitt , Michael (Maomian) Fan , Shelly D. Roper
IPC分类号： C12Q1/70 , C12Q1/68 , G01N33/566
CPC分类号： C12Q1/6811 , C12N15/115 , Y10T436/143333 , C12Q2525/205 , C12Q2565/101
摘要： Embodiments herein relate to compositions and methods for making and using aptamers, for example, DNA aptamers (DCEs) and/or RNA aptamers. In some embodiments, methods relate to making and amplifying target DCEs. In certain embodiments, methods for making capture elements or aptamers concern using a reporter moiety and signal reducing moiety prior to amplifying a target-specific capture element. In some embodiments, methods disclosed herein may be used to rapidly generate large quantities of aptamers such as DCEs directed to a particular target agent. Some embodiments relate to systems for performing automated generation of aptamers.
摘要翻译：本文的实施方案涉及用于制备和使用适体的组合物和方法，例如DNA适体（DCE）和/或RNA适体。在一些实施方案中，方法涉及制备和扩增目标DCE。在某些实施方案中，用于制备捕获元件或适体的方法在放大靶特异性捕获元件之前关注使用报道部分和信号降低部分。在一些实施方案中，本文公开的方法可用于快速产生大量适配体，例如针对特定目标试剂的DCE。一些实施例涉及用于执行适配体的自动生成的系统。

9. 发明授权

US06303316B1 Organic semiconductor recognition complex and system 有权
标题翻译：有机半导体识别复杂系统
公开(公告)号：US06303316B1
公开(公告)日：2001-10-16
申请号：US09608706
申请日：2000-06-30
申请人： Johnathan L. Kiel , John G. Bruno , Jill E. Parker , John L. Alls , Charles R. Batishko , Eric A. Holwitt
发明人： Johnathan L. Kiel , John G. Bruno , Jill E. Parker , John L. Alls , Charles R. Batishko , Eric A. Holwitt
IPC分类号： C12Q168
CPC分类号： C12Q1/6825 , C12Q1/6837 , Y10T436/143333 , C12Q2565/607
摘要： In a recognition complex system, nucleic acid ligands comprising random DNA sequences are operatively coupled to an organic semiconductor and distributed so as to form an array of recognition complexes. When an unknown chemical or biological analyte is applied to the array, the electrical and/or photochemical properties of one or more of the recognition complexes are altered upon binding of the nucleic acid ligand to the analyte. The degree to which the electrical and/or photochemical properties change is a function of the affinity of the nucleic acid ligand sequence for the analyte. The electrical and photochemical changes associated with the array, as a whole, can be used as a unique signature to identify the analyte. In certain embodiments, an iterative process of selection and amplification of nucleic acid ligands that bind to the analyte can be used to generate a new array with greater affinity and specificity for a target analyte, or to produce one or more nucleic acid ligands with high binding affinity for an analyte. The present invention also provides methods for preparing nucleic acid ligands that bind with high affinity to an analyte and using such nucleic acid ligands to neutralize the analyte.
摘要翻译：在识别复合体系中，包含随机DNA序列的核酸配体可操作地偶联到有机半导体上，并分布以形成识别复合物的阵列。当将未知的化学或生物分析物施加到阵列时，一个或多个识别复合物的电和/或光化学性质在核酸配体与分析物结合时被改变。电和/或光化学性质变化的程度是核酸配体序列对分析物的亲和力的函数。与阵列相关联的电学和光化学变化作为一个整体，可以用作识别分析物的独特标记。在某些实施方案中，结合分析物的核酸配体的选择和扩增的迭代过程可用于产生对靶分析物具有更大亲和性和特异性的新阵列，或产生具有高结合力的一个或多个核酸配体对分析物的亲和力。本发明还提供了制备以高亲和力结合分析物并使用这种核酸配体中和分析物的核酸配体的方法。

10. 发明授权

US06379900B1 Compositions and methods of use of 8-nitroguanine 有权
标题翻译： 8-硝基鸟嘌呤的组成和使用方法
公开(公告)号：US06379900B1
公开(公告)日：2002-04-30
申请号：US09850646
申请日：2001-05-07
申请人： Eric A. Holwitt
发明人： Eric A. Holwitt
IPC分类号： C12Q168
CPC分类号： G01N33/5091
摘要： Novel methods for the synthesis of 8-nitroguanine are provided. Compositions comprising 8-nitroguanine, made by the novel synthetic methods are also provided herein. Methods of use of 8-nitroguanine, made by the novel synthetic methods, as a standard for detection of 8-nitroguanine in samples are also encompassed within the scope of the present invention. The present invention further concerns methods of predicting organ transplant rejection and detecting exposure to environmental stressors, such as ionizing radiation, toxic chemicals or infectious agents, by detecting 8-nitroguanine in one or more samples from a transplant recipient or an organism exposed to stress.
摘要翻译：提供了用于合成8-硝基鸟嘌呤的新方法。本文还提供了由新型合成方法制备的包含8-硝基鸟嘌呤的组合物。通过新型合成方法制备的8-硝基鸟嘌呤的方法作为检测样品中8-硝基鸟嘌呤的标准也包括在本发明的范围内。本发明还涉及通过检测来自移植受体或暴露于应激的生物体的一种或多种样品中的8-硝基鸟嘌呤来预测器官移植排斥和检测暴露于环境胁迫因子如电离辐射，有毒化学物质或感染因子的方法。

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