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1. 发明申请

US20070161000A1 Plasmid purification 有权
标题翻译：质粒纯化
公开(公告)号：US20070161000A1
公开(公告)日：2007-07-12
申请号：US10589740
申请日：2005-02-21
申请人： James Van Alstine , Jan Bergstrom , Dag Lindstrom , Joachim Stadler
发明人： James Van Alstine , Jan Bergstrom , Dag Lindstrom , Joachim Stadler
IPC分类号： C12Q1/68
CPC分类号： C12N15/1006 , B01D15/327 , B01D15/34 , B01D15/363 , B01J41/20
摘要： The present invention relates to a method of isolating at least one plasmid from other components of a liquid, which method comprises the steps of providing a separation matrix comprised of one or more porous carriers, which carrier(s) present anion exchange groups on external surfaces as well as pore surfaces and a pore size distribution that does not allow access of plasmids to pore surfaces; contacting said matrix with the liquid to allow adsorption of the plasmids to ligands present on the separation matrix; contacting an eluent with the separation matrix to release the plasmids and recovering plasmids from a fraction of said eluent. Thus, the present method allows the plasmids to adsorb to the external surfaces of the matrix, while other components such as RNA is adsorbed onto the pore surfaces. In one embodiment, the matrix presents a DNA exclusion limit of at least about 270 base pairs; such as at least about 1,000 base pairs.
摘要翻译：本发明涉及从液体的其它组分分离至少一种质粒的方法，该方法包括以下步骤：提供由一种或多种多孔载体组成的分离基质，该载体在外表面上存在阴离子交换基团以及孔表面和不允许质粒进入孔表面的孔径分布; 使所述基质与液体接触以允许质粒吸附到存在于分离基质上的配体; 将洗脱液与分离基质接触以释放质粒并从一部分所述洗脱液中回收质粒。因此，本方法允许质粒吸附到基质的外表面，而诸如RNA的其它组分被吸附到孔表面上。在一个实施方案中，所述基质呈现至少约270个碱基对的DNA排除极限; 例如至少约1,000个碱基对。

2. 发明授权

US08093373B2 Plasmid purification 有权
标题翻译：质粒纯化
公开(公告)号：US08093373B2
公开(公告)日：2012-01-10
申请号：US10589740
申请日：2005-02-21
申请人： James Van Alstine , Jan Bergström , Dag Lindström , Joachim Stadler
发明人： James Van Alstine , Jan Bergström , Dag Lindström , Joachim Stadler
IPC分类号： C07H21/00
CPC分类号： C12N15/1006 , B01D15/327 , B01D15/34 , B01D15/363 , B01J41/20
摘要： The present invention relates to a method of isolating at least one plasmid from other components of a liquid, which method comprises the steps of providing a separation matrix comprised of one or more porous carriers, which carrier(s) present anion exchange groups on external surfaces as well as pore surfaces and a pore size distribution that does not allow access of plasmids to pore surfaces; contacting said matrix with the liquid to allow adsorption of the plasmids to ligands present on the separation matrix; contacting an eluent with the separation matrix to release the plasmids and recovering plasmids from a fraction of said eluent. Thus, the present method allows the plasmids to adsorb to the external surfaces of the matrix, while other components such as RNA is adsorbed onto the pore surfaces. In one embodiment, the matrix presents a DNA exclusion limit of at least about 270 base pairs; such as at least about 1,000 base pairs.
摘要翻译：本发明涉及从液体的其它组分分离至少一种质粒的方法，该方法包括以下步骤：提供由一种或多种多孔载体组成的分离基质，该载体在外表面上存在阴离子交换基团以及孔表面和不允许质粒进入孔表面的孔径分布; 使所述基质与液体接触以允许质粒吸附到存在于分离基质上的配体; 将洗脱液与分离基质接触以释放质粒并从一部分所述洗脱液中回收质粒。因此，本方法允许质粒吸附到基质的外表面，而诸如RNA的其它组分被吸附到孔表面上。在一个实施方案中，所述基质呈现至少约270个碱基对的DNA排除极限; 例如至少约1,000个碱基对。

3. 发明授权

US06916919B2 Isolation of DNA molecules 有权
标题翻译：分离DNA分子
公开(公告)号：US06916919B2
公开(公告)日：2005-07-12
申请号：US10473239
申请日：2002-04-15
申请人： Raf Lemmens , Tomas Nyhammar , Jan Berglof , Joachim Stadler
发明人： Raf Lemmens , Tomas Nyhammar , Jan Berglof , Joachim Stadler
IPC分类号： G01N30/06 , B01D15/38 , B01J20/281 , B01J20/283 , B01J20/285 , B01J20/286 , C12N15/09 , C12N15/10 , C12Q1/68 , G01N30/26 , G01N30/50 , G01N30/80 , G01N30/88 , C07H21/00 , C08G63/68 , C08G69/14 , C08G69/26 , C07G37/00
CPC分类号： B01D15/3804 , B01J20/286 , C12N15/101
摘要： The present invention relates to a process for separating nucleic acid molecules, preferably open circular and supercoiled plasmid DNA and RNA molecules from each other, comprising the steps of providing a solution comprising the molecules; adsorbing the molecules to adsorbing groups on a carrier; and optionally washing the column with a suitable solution. The present process is especially suitable for large-scale isolation of supercoiled ccc DNA to be used in gene therapy.
摘要翻译：本发明涉及将核酸分子，优选开环和超螺旋质粒DNA和RNA分子彼此分离的方法，包括提供包含分子的溶液的步骤; 吸附分子吸附载体上的基团; 并任选地用合适的溶液洗涤柱。本方法特别适用于大规模分离用于基因治疗的超螺旋ccc DNA。

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