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    • 1. 发明申请
    • Plasmid purification
    • 质粒纯化
    • US20070161000A1
    • 2007-07-12
    • US10589740
    • 2005-02-21
    • James Van AlstineJan BergstromDag LindstromJoachim Stadler
    • James Van AlstineJan BergstromDag LindstromJoachim Stadler
    • C12Q1/68
    • C12N15/1006B01D15/327B01D15/34B01D15/363B01J41/20
    • The present invention relates to a method of isolating at least one plasmid from other components of a liquid, which method comprises the steps of providing a separation matrix comprised of one or more porous carriers, which carrier(s) present anion exchange groups on external surfaces as well as pore surfaces and a pore size distribution that does not allow access of plasmids to pore surfaces; contacting said matrix with the liquid to allow adsorption of the plasmids to ligands present on the separation matrix; contacting an eluent with the separation matrix to release the plasmids and recovering plasmids from a fraction of said eluent. Thus, the present method allows the plasmids to adsorb to the external surfaces of the matrix, while other components such as RNA is adsorbed onto the pore surfaces. In one embodiment, the matrix presents a DNA exclusion limit of at least about 270 base pairs; such as at least about 1,000 base pairs.
    • 本发明涉及从液体的其它组分分离至少一种质粒的方法,该方法包括以下步骤:提供由一种或多种多孔载体组成的分离基质,该载体在外表面上存在阴离子交换基团 以及孔表面和不允许质粒进入孔表面的孔径分布; 使所述基质与液体接触以允许质粒吸附到存在于分离基质上的配体; 将洗脱液与分离基质接触以释放质粒并从一部分所述洗脱液中回收质粒。 因此,本方法允许质粒吸附到基质的外表面,而诸如RNA的其它组分被吸附到孔表面上。 在一个实施方案中,所述基质呈现至少约270个碱基对的DNA排除极限; 例如至少约1,000个碱基对。
    • 2. 发明授权
    • Plasmid purification
    • 质粒纯化
    • US08093373B2
    • 2012-01-10
    • US10589740
    • 2005-02-21
    • James Van AlstineJan BergströmDag LindströmJoachim Stadler
    • James Van AlstineJan BergströmDag LindströmJoachim Stadler
    • C07H21/00
    • C12N15/1006B01D15/327B01D15/34B01D15/363B01J41/20
    • The present invention relates to a method of isolating at least one plasmid from other components of a liquid, which method comprises the steps of providing a separation matrix comprised of one or more porous carriers, which carrier(s) present anion exchange groups on external surfaces as well as pore surfaces and a pore size distribution that does not allow access of plasmids to pore surfaces; contacting said matrix with the liquid to allow adsorption of the plasmids to ligands present on the separation matrix; contacting an eluent with the separation matrix to release the plasmids and recovering plasmids from a fraction of said eluent. Thus, the present method allows the plasmids to adsorb to the external surfaces of the matrix, while other components such as RNA is adsorbed onto the pore surfaces. In one embodiment, the matrix presents a DNA exclusion limit of at least about 270 base pairs; such as at least about 1,000 base pairs.
    • 本发明涉及从液体的其它组分分离至少一种质粒的方法,该方法包括以下步骤:提供由一种或多种多孔载体组成的分离基质,该载体在外表面上存在阴离子交换基团 以及孔表面和不允许质粒进入孔表面的孔径分布; 使所述基质与液体接触以允许质粒吸附到存在于分离基质上的配体; 将洗脱液与分离基质接触以释放质粒并从一部分所述洗脱液中回收质粒。 因此,本方法允许质粒吸附到基质的外表面,而诸如RNA的其它组分被吸附到孔表面上。 在一个实施方案中,所述基质呈现至少约270个碱基对的DNA排除极限; 例如至少约1,000个碱基对。