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1. 发明申请

US20070161000A1 Plasmid purification 有权
标题翻译：质粒纯化
公开(公告)号：US20070161000A1
公开(公告)日：2007-07-12
申请号：US10589740
申请日：2005-02-21
申请人： James Van Alstine , Jan Bergstrom , Dag Lindstrom , Joachim Stadler
发明人： James Van Alstine , Jan Bergstrom , Dag Lindstrom , Joachim Stadler
IPC分类号： C12Q1/68
CPC分类号： C12N15/1006 , B01D15/327 , B01D15/34 , B01D15/363 , B01J41/20
摘要： The present invention relates to a method of isolating at least one plasmid from other components of a liquid, which method comprises the steps of providing a separation matrix comprised of one or more porous carriers, which carrier(s) present anion exchange groups on external surfaces as well as pore surfaces and a pore size distribution that does not allow access of plasmids to pore surfaces; contacting said matrix with the liquid to allow adsorption of the plasmids to ligands present on the separation matrix; contacting an eluent with the separation matrix to release the plasmids and recovering plasmids from a fraction of said eluent. Thus, the present method allows the plasmids to adsorb to the external surfaces of the matrix, while other components such as RNA is adsorbed onto the pore surfaces. In one embodiment, the matrix presents a DNA exclusion limit of at least about 270 base pairs; such as at least about 1,000 base pairs.
摘要翻译：本发明涉及从液体的其它组分分离至少一种质粒的方法，该方法包括以下步骤：提供由一种或多种多孔载体组成的分离基质，该载体在外表面上存在阴离子交换基团以及孔表面和不允许质粒进入孔表面的孔径分布; 使所述基质与液体接触以允许质粒吸附到存在于分离基质上的配体; 将洗脱液与分离基质接触以释放质粒并从一部分所述洗脱液中回收质粒。因此，本方法允许质粒吸附到基质的外表面，而诸如RNA的其它组分被吸附到孔表面上。在一个实施方案中，所述基质呈现至少约270个碱基对的DNA排除极限; 例如至少约1,000个碱基对。

2. 发明申请

US20090281288A1 MATRIX FOR SEPARATION OF POLYETHERS AND METHOD OF SEPARATION 有权
标题翻译：用于分离聚合物的基质和分离方法
公开(公告)号：US20090281288A1
公开(公告)日：2009-11-12
申请号：US12506445
申请日：2009-07-21
申请人： JAMES VAN ALSTINE , Jan Bergstrom , Henrik Ihre , Tobias Soderman
发明人： JAMES VAN ALSTINE , Jan Bergstrom , Henrik Ihre , Tobias Soderman
IPC分类号： C07K1/14 , C07C29/74
CPC分类号： B01J20/3278 , B01D15/32 , B01D15/362 , B01D15/38 , B01D15/424 , B01J20/286 , B01J20/288 , B01J20/321 , B01J20/3212 , B01J20/327 , B01J20/328
摘要： The present invention relates to a separation matrix comprised of a support to the surfaces of which polymer chains have been coupled, wherein each polymer chain presents recurring proton-donating groups and at least the surface of the support is substantially hydrophilic. In the most advantageous embodiment, the support is porous cross-linked agarose, the polymers are poly(acrylic acid) and the proton-donating groups are carboxyl groups. The matrix is useful e.g. to remove PEG from pegylated and/or native compounds in a liquid. Accordingly, the invention also encompasses a method, such as a chromatographic method, wherein the separation matrix according to the invention is used, for example as a pre-treatment of a reaction mixture that comprises unreacted PEG, pegylated proteins and native proteins.
摘要翻译：本发明涉及一种分离基质，其由其上已经连接有聚合物链的表面的载体组成，其中每个聚合物链呈现重复的质子供体基团，并且载体的至少表面基本上是亲水的。在最有利的实施方案中，载体是多孔交联琼脂糖，聚合物是聚（丙烯酸），质子供体基团是羧基。该矩阵是有用的。以从液体中的聚乙二醇化和/或天然化合物中除去PEG。因此，本发明还包括诸如色谱方法的方法，其中使用根据本发明的分离基质，例如作为包含未反应的PEG，聚乙二醇化蛋白质和天然蛋白质的反应混合物的预处理。

3. 发明授权

US08092682B2 Matrix for separation of polyethers and method of separation 有权
标题翻译：用于分离聚醚的基质和分离方法
公开(公告)号：US08092682B2
公开(公告)日：2012-01-10
申请号：US12506445
申请日：2009-07-21
申请人： James Van Alstine , Jan Bergstrom , Henrik Ihre , Tobias Soderman
发明人： James Van Alstine , Jan Bergstrom , Henrik Ihre , Tobias Soderman
IPC分类号： B01D15/08
CPC分类号： B01J20/3278 , B01D15/32 , B01D15/362 , B01D15/38 , B01D15/424 , B01J20/286 , B01J20/288 , B01J20/321 , B01J20/3212 , B01J20/327 , B01J20/328
摘要： The present invention relates to a separation matrix comprised of a support to the surfaces of which polymer chains have been coupled, wherein each polymer chain presents recurring proton-donating groups and at least the surface of the support is substantially hydrophilic. In the most advantageous embodiment, the support is porous cross-linked agarose, the polymers are poly(acrylic acid) and the proton-donating groups are carboxyl groups. The matrix is useful e.g. to remove PEG from pegylated and/or native compounds in a liquid. Accordingly, the invention also encompasses a method, such as a chromatographic method, wherein the separation matrix according to the invention is used, for example as a pre-treatment of a reaction mixture that comprises unreacted PEG, pegylated proteins and native proteins.
摘要翻译：本发明涉及一种分离基质，其由其上已经连接有聚合物链的表面的载体组成，其中每个聚合物链呈现重复的质子供体基团，并且载体的至少表面基本上是亲水的。在最有利的实施方案中，载体是多孔交联琼脂糖，聚合物是聚（丙烯酸），质子供体基团是羧基。该矩阵是有用的。以从液体中的聚乙二醇化和/或天然化合物中除去PEG。因此，本发明还包括诸如色谱方法的方法，其中使用根据本发明的分离基质，例如作为包含未反应的PEG，聚乙二醇化蛋白质和天然蛋白质的反应混合物的预处理。

4. 发明授权

US08070958B2 Separation matrix having a ligand density gradient 失效
标题翻译：具有配体密度梯度的分离基质
公开(公告)号：US08070958B2
公开(公告)日：2011-12-06
申请号：US12692671
申请日：2010-01-25
申请人： Jan Bergstrom , Gunnar Glad , Bo-Lennart Johansson , Anders Ljunglof , Jean-Luc Maloisel
发明人： Jan Bergstrom , Gunnar Glad , Bo-Lennart Johansson , Anders Ljunglof , Jean-Luc Maloisel
IPC分类号： B01D15/08 , C02F1/28
CPC分类号： B01J20/3285 , B01D15/364 , B01J20/286 , B01J20/3244
摘要： The present invention relates to a separation matrix comprised of ligands coupled to the surfaces of a porous support, such as one or more porous particles, wherein the ligands provide at least one chemical gradient within the support. In the most advantageous embodiment, the chemical gradient is a ligand density gradient. The invention also relates to a method of providing a separation matrix comprising ligands coupled to the surfaces of a porous support, in which method at least one ligand density gradient is provided by solvent-controlled diffusion of at least one reagent into the porous support.
摘要翻译：本发明涉及由耦合到多孔载体的表面的配体（例如一种或多种多孔颗粒）组成的分离基质，其中配体在载体内提供至少一种化学梯度。在最有利的实施方案中，化学梯度是配体密度梯度。本发明还涉及一种提供包含耦合到多孔载体表面的配体的分离基质的方法，其中至少一种配体密度梯度由至少一种试剂的溶剂控制扩散提供到多孔载体中。

5. 发明申请

US20070125711A1 Novel separation matrix 审中-公开
标题翻译：新型分离矩阵
公开(公告)号：US20070125711A1
公开(公告)日：2007-06-07
申请号：US10575945
申请日：2004-11-01
申请人： Jan Bergstrom , Gunnar Glad , Bo-Lennart Johansson , Anders Ljunglof , Jean-Luc Maloisel
发明人： Jan Bergstrom , Gunnar Glad , Bo-Lennart Johansson , Anders Ljunglof , Jean-Luc Maloisel
IPC分类号： B01D15/08
CPC分类号： B01J20/3285 , B01D15/364 , B01J20/286 , B01J20/3244
摘要： The present invention relates to a separation matrix comprised of ligands coupled to the surfaces of a porous support, such as one or more porous particles, wherein the ligands provide at least one chemical gradient within the support. In the most advantageous embodiment, the chemical gradient is a ligand density gradient. The invention also relates to a method of providing a separation matrix comprising ligands coupled to the surfaces of a porous support, in which method at least one ligand density gradient is provided by solvent-controlled diffusion of at least one reagent into the porous support.
摘要翻译：本发明涉及由耦合到多孔载体的表面的配体（例如一种或多种多孔颗粒）组成的分离基质，其中配体在载体内提供至少一种化学梯度。在最有利的实施方案中，化学梯度是配体密度梯度。本发明还涉及一种提供包含耦合到多孔载体表面的配体的分离基质的方法，其中至少一种配体密度梯度由至少一种试剂的溶剂控制扩散提供到多孔载体中。

6. 发明申请

US20130244229A1 BUFFERING COMPOSITIONS ENCLOSED IN A SIZE EXCLUSION MATRIX 审中-公开
标题翻译：缓冲组合物在一个尺寸排除矩阵中
公开(公告)号：US20130244229A1
公开(公告)日：2013-09-19
申请号：US13990765
申请日：2011-11-29
申请人： Jan Bergstrom , Bo-Lennart Johansson
发明人： Jan Bergstrom , Bo-Lennart Johansson
IPC分类号： G01N1/28
CPC分类号： G01N1/28 , B01J20/28009 , B01J20/286 , B01J20/3208 , B01J20/3217 , B01J20/327 , B01J20/3274 , B01J20/3285 , B01J20/3293 , Y10T436/2525
摘要： The present invention relates to a buffering composition, more closely a composition comprising porous matrix-enclosed buffering agent(s) giving a stabilisation of pH when applied in for example aqueous environment.The composition comprises buffering agent(s) enclosed in a first porous matrix with an impenetrability corresponding to a fractionation range for globular proteins and peptides of
摘要翻译：本发明涉及一种缓冲组合物，更接近地包含当在例如水性环境中施用时提供pH稳定化的多孔基质封闭的缓冲剂的组合物。组合物包含封闭在第一多孔基质中的缓冲剂，其不透性对应于球状蛋白和肽的分数范围<10000g / mol，优选<5000g / mol，更优选<700g / mol。

7. 发明授权

US5436161A Matrix coating for sensing surfaces capable of selective biomolecular interactions, to be used in biosensor systems 失效
标题翻译：用于检测能够选择性生物分子相互作用的表面的基质涂层，用于生物传感器系统中
公开(公告)号：US5436161A
公开(公告)日：1995-07-25
申请号：US279089
申请日：1994-07-22
申请人： Jan Bergstrom , Stefan Lofaas , Bo Johnsson
发明人： Jan Bergstrom , Stefan Lofaas , Bo Johnsson
IPC分类号： C12Q1/00 , G01N33/543 , G01N33/547 , G01N33/548 , G01N33/553 , G01N21/00 , C12M1/34
CPC分类号： C12Q1/002 , G01N33/54353 , G01N33/54393 , G01N33/553 , B82Y15/00 , B82Y30/00 , Y10S435/808
摘要： A matrix coating suitable for use in a biosensor is provided. This matrix coating comprises a hydrogel bound to a surface and via which a desired ligand can be bound. This hydrogel is activated to contain charged groups for bringing about the concentration of biomolecules carrying an opposite charge to that of said charged groups, and reactive groups for covalently binding the biomolecules concentrated to the matrix coating.
摘要翻译：提供适用于生物传感器的基质涂层。该基质涂层包含与表面结合的水凝胶，通过该水凝胶可以结合所需的配体。该水凝胶被活化以含有带电基团，以使带有与所述带电基团相反的电荷的生物分子的浓度，以及用于共价结合浓缩到基质涂层的生物分子的反应性基团。

8. 发明授权

US5242828A Sensing surfaces capable of selective biomolecular interactions, to be used in biosensor systems 失效
标题翻译：可用于生物传感器系统中选择性生物分子相互作用的感应表面
公开(公告)号：US5242828A
公开(公告)日：1993-09-07
申请号：US681531
申请日：1991-05-10
申请人： Jan Bergstrom , Stefan Lofas , Bo Johnsson
发明人： Jan Bergstrom , Stefan Lofas , Bo Johnsson
IPC分类号： C12Q1/00 , G01N33/543 , G01N33/547 , G01N33/548 , G01N33/553
CPC分类号： C12Q1/002 , G01N33/54353 , G01N33/54393 , G01N33/553 , B82Y15/00 , B82Y30/00 , Y10S435/808
摘要： A sensing surface suitable for use in a biosensor, comprising:a film of a free electron metal selected from the group consisting of copper, silver, aluminum and gold; anda densely packed monolayer of an organic molecule X-R-Y coated on one of the faces of said film where X is a group selected from the group consisting ofasymmetrical or symmetrical disulfide (--SSR'Y', -SSRY, sulfide (-SR'Y', -SRY), diselenide (-SeSeR'Y', -SeSeRY), selenide (SeR'Y', -SeRY),thiol (-SH), isonitrile, nitro (-NO.sub.2), selenol (-SeH), trivalent phosphorus compounds, isothiocyanate, xanthate, thiocarbamate, phosphine,thio acid and dithio acid (-COSH, -CSSH)where R and R' are hydrocarbon chains optionally interrupted by hetero atoms, of a length exceeding 10 atoms, wherein in the case of an asymmetrical molecule, R' or R may be H, and Y and Y' are active groups for covalently binding ligands or a biocompatible porous matrix.
摘要翻译： PCT No.PCT / SE89 / 00642 Sec。 371日期1991年5月10日 102（e）日期1991年5月10日PCT提交1989年11月9日PCT公布。出版物WO90 / 05303 日期1990年5月17日。适用于生物传感器的感测表面，包括：选自铜，银，铝和金的自由电子金属的膜; 和在所述膜的一个表面上涂覆的有机分子XRY的密集填充单层，其中X是选自不对称或对称二硫化物（-SSR'Y'，-SSRY，硫化物（-SR'Y）（-Se），硒化物（-SeSeR'Y'，-SeSeRY），硒化物（SeR'Y'，-SeRY），硫醇（-SH），异腈，硝基（-NO 2），硒（-SeH）磷化合物，异硫氰酸酯，黄原酸酯，硫代氨基甲酸酯，膦，硫代酸和二硫代酸（-COSH，-CSSH），其中R和R'是长度超过10个原子的任选被杂原子中断的烃链，其中在不对称分子，R'或R可以是H，Y和Y'是用于共价结合配体或生物相容性多孔基质的活性基团。

9. 发明授权

US08574437B2 Method for production of chromatography media 失效
标题翻译：色谱介质的制备方法
公开(公告)号：US08574437B2
公开(公告)日：2013-11-05
申请号：US13575750
申请日：2011-02-16
申请人： Jan Bergstrom , Bo-Lennart Johansson
发明人： Jan Bergstrom , Bo-Lennart Johansson
IPC分类号： B01D15/08
CPC分类号： B01J20/3293 , B01D15/361 , B01D15/3804 , B01J20/28004 , B01J20/286 , B01J20/3206 , B01J20/3248 , B01J20/3285 , B01J39/26 , B01J41/20 , C07K1/18
摘要： The present invention relates to a method of producing novel chromatography media and use thereof for purification of biomolecules, such as proteins. The chromatography media comprises shell beads having an inner porous core and an outer shell. The method comprises providing buffering ligands in the core of the beads, and providing binding ligands aimed for biomolecule binding in the outer shell of the beads. This method makes it possible to optimize binding properties and buffering properties independently of each other which is especially to advantageous for production of chromatofocusing media.
摘要翻译：本发明涉及生产新型色谱介质的方法及其用于生物分子如蛋白质的纯化的用途。色谱介质包括具有内多孔芯和外壳的壳珠。该方法包括在珠的核心中提供缓冲配体，并提供靶向用于珠粒外壳中的生物分子结合的结合配体。该方法使得可以彼此独立地优化结合性质和缓冲性质，这特别有利于生产色谱聚焦介质。

10. 发明申请

US20050267295A1 Method for selective removal of a substance from samples containing compounds having nucleic acid structure 审中-公开
标题翻译：从含有具有核酸结构的化合物的样品中选择性去除物质的方法
公开(公告)号：US20050267295A1
公开(公告)日：2005-12-01
申请号：US11076477
申请日：2005-03-09
申请人： Makonnen Belew , Jan Bergstrom , Rolf Berglund , Lennart Soderberg
发明人： Makonnen Belew , Jan Bergstrom , Rolf Berglund , Lennart Soderberg
IPC分类号： C12N15/09 , B01D15/00 , B01D15/08 , B01D15/36 , B01J20/32 , B01J41/20 , C12N15/10 , C07H21/04
CPC分类号： B01J20/3293 , B01D15/363 , B01J20/321 , B01J20/3219 , B01J20/3248 , B01J20/3251 , B01J20/3253 , B01J41/20 , C12N15/1003
摘要： A method for purifying a desired substance by separating from each other a substance (I) from a substance (II), one of which is the desired substance, both of which have affinity for the same ligand structure, and wherein substance (I) is smaller than substance (II). The method comprising the steps of: (i) providing substances I and II in a liquid; (ii) contacting the liquid with an adsorbent which selectively adsorbs substance I; (iii) recovering the desired substance; The adsorbent has (a) an interior part which carries a ligand structure that is capable of binding to substances I and II, and is accessible to substance I, and (b) an outer surface layer that does not adsorb substance II, and is more easily penetrated by substance I than by substance II.
摘要翻译：通过从物质（II）彼此分离出物质（I）的方法，其中一种物质（II）是所需物质，两者对相同的配体结构具有亲和性，并且其中物质（I）为小于物质（II）。该方法包括以下步骤：（i）在液体中提供物质I和II; （ii）使液体与选择性吸附物质I的吸附剂接触; （iii）回收所需物质; 吸附剂具有（a）内部部分，其携带能够结合物质I和II的物质I可接近的配体结构，和（b）不吸附物质II的外表面层，并且更多物质I易于被物质II渗透。

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