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1. 发明公开

US20240226357A1 Sanitization Method for Affinity Chromatography Matrices 审中-公开
公开(公告)号：US20240226357A1
公开(公告)日：2024-07-11
申请号：US18604231
申请日：2024-03-13
申请人： Cytiva BioProcess R&D AB
发明人： Elin Marianne Monie , Tomas Bjorkman , Anna Gronberg , Anders Ljunglof , Gustav Jose Rodrigo , Karin Torstenson , Magnus Carl Erik Wetterhall
IPC分类号： A61L2/18 , B01D15/20 , B01D15/38 , G01N30/50
CPC分类号： A61L2/18 , B01D15/203 , B01D15/3804 , G01N30/50
摘要： The invention discloses a method for cleaning or sanitization of an affinity chromatography matrix, comprising the steps of:
a) providing an affinity chromatography matrix having oxidation-tolerant proteinaceous ligands coupled to a support,
b) contacting the matrix with a sanitization solution comprising at least one oxidant defined by formula I,

wherein R is hydrogen or an acyl group R′—C(O)—, with R′ being a hydrogen or a methyl, ethyl or propyl group.

2. 发明授权

US11519851B2 Monitoring of compounds 有权
公开(公告)号：US11519851B2
公开(公告)日：2022-12-06
申请号：US16332527
申请日：2017-09-15
申请人： C Technologies Inc.
发明人： Craig Harrison , Ramsey Shanbaky
IPC分类号： G01N21/33 , G01N21/03 , G01N21/05 , B01D15/20 , B01D35/02 , G01N30/50 , G01N21/27 , G01N30/74 , G01N30/88 , B01D65/10 , B01D57/02 , G01N27/447 , G01N30/46 , B01D61/20 , G01N21/17
摘要： The present invention relates to methods for determining the change in concentration of a substance in solution over time by continuously monitoring in real time. In particular, the present invention relates to methods for continuously monitoring the concentration of compounds during the manufacturing process of biomolecules.

3. 发明授权

US11280766B2 Encapsulated pre-analytic workflows for flow-through devices, liquid chromatography and mass spectrometric analysis 有权
公开(公告)号：US11280766B2
公开(公告)日：2022-03-22
申请号：US16321222
申请日：2017-07-28
申请人： Waters Technologies Corporation
发明人： Kevin Daniel Wyndham , Darryl W. Brousmiche
IPC分类号： G01N30/06 , G01N30/50 , G01N30/72 , G01N30/88 , G01N30/52
摘要： This invention relates to encapsulated workflow reagents comprising an encapsulating material and a workflow reagent encapsulated within the encapsulating material for sample and workflow preparation prior to chromatographic, spectroscopic or other analytical systems, use thereof, and devices comprising the same.

4. 发明授权

US11161057B2 Device for solid phase extraction and method for use thereof 有权
公开(公告)号：US11161057B2
公开(公告)日：2021-11-02
申请号：US15536506
申请日：2015-12-16
申请人： Waters Technologies Corporation
发明人： Pamela C. Iraneta , Xin Zhang , Frank John Marszalkowski, Jr.
IPC分类号： B01D15/38 , B01J20/28 , B01J20/32 , G01N30/72 , G01N30/50 , B01J20/287 , B01J20/288 , G01N30/00 , G01N1/40
摘要： Disclosed is a device for a solid phase extraction comprising two or more of the sorbents to remove phospholipids and salts from a sample, to thereby eliminate matrix effects during mass spectrometry analysis. In particular, the sorbents includes at least one sorbent which is water-wettable and contains at least one hydrophobic component and at least one hydrophilic component and at least one of sorbent having a specific affinity for a matrix interference like phospholipids. Further disclosed is a method using the device of the present invention.

5. 发明授权

US10308925B2 Methionine lyase, encoding gene and biosynthetic method thereof 有权
公开(公告)号：US10308925B2
公开(公告)日：2019-06-04
申请号：US15127903
申请日：2015-12-03
申请人： Hubei University Of Technology
发明人： Yajie Tang , Kaizhi Jia , Yanghua Xu , Quan Zhang , Hongmei Li
IPC分类号： C12N15/70 , C12N15/74 , C12N9/88 , C12N15/81 , G01N30/50 , G01N30/80 , G01N27/447
摘要： Embodiment of the present invention discloses a kind of methionine lyase and its encoding gene and biosynthetic method. According to the present invention, the gene encoding methionine lyase as shown in SEQ ID No. 1 is separated from the genome of C. rosea. Embodiment of the present invention further provides an efficient biosynthetic method of methionine lyase, comprising: (1) cloning gene (shown in SEQ ID No.1) encoding methionine degradation enzyme into a yeast expression vector to construct recombinant yeast expression vector; (2) transforming the recombinant yeast expression vector into Saccharomyces cerevisiae to obtain expression strain; (3) inducing the expression strain to express the methionine lyase, collecting induced strains, purifying expressed recombinant methionine lyase. Purity of recombinant methionine lyase prepared according to the present invention is above 90%, and its efficiency of degradating methionine can reach 0.53±0.0030 μM MTL·h−1·mg protein−1.

6. 发明授权

US10302604B2 Solvent delivery device and liquid chromatograph 有权
公开(公告)号：US10302604B2
公开(公告)日：2019-05-28
申请号：US13783922
申请日：2013-03-04
申请人： SHIMADZU CORPORATION
发明人： Keisuke Ogawa , Yoshiaki Maeda
IPC分类号： G01N30/34 , G01N30/50 , G01N30/24
摘要： A solvent delivery device includes a solvent delivery pump and a flow path switching valve. The flow path switching valve is connected to downstream ends of mobile phase sending flow paths and the solvent delivery pump is to be connected to one of the mobile phase sending flow paths by switching the flow path switching valve. At least one of the mobile phase sending flow paths includes a buffer solution sending flow path. The buffer solution sending flow path includes a butler solution storage section, a cleaning solution storage section, and a mobile phase switching valve for switching connection of the flow path switching valve to one of the storage sections.

7. 发明授权

US09504936B2 Method of packing chromatographic columns 有权
标题翻译：色谱柱的包装方法
公开(公告)号：US09504936B2
公开(公告)日：2016-11-29
申请号：US13639165
申请日：2011-04-05
申请人： Mary Jean Wirth , Sampath Ranasinghe Kodithuwakkuge , Charu Yerneni , Robert Birdsall , Tharanga Ranasinghe Kodituwakkuge
发明人： Mary Jean Wirth , Sampath Ranasinghe Kodithuwakkuge , Charu Yerneni , Robert Birdsall
IPC分类号： B01D15/20 , G01N30/56 , G01N30/50 , G01N30/52 , B01J20/10 , B01J20/283 , B01J20/30 , B01D15/26 , B01D15/16 , B01D15/30 , B01D15/32 , G01N30/88
CPC分类号： B01D15/206 , B01D15/168 , B01D15/305 , B01D15/325 , B01J20/283 , G01N30/56 , G01N2030/562 , G01N2030/565 , G01N2030/8831
摘要： Improved methods for packing a chromatographic column and columns packed by these methods are provided. The methods include introducing a packing material into a chromatographic column, while simultaneously ultrasonicating and applying pressure to the packing material to form a packed column with radial homogeneity.
摘要翻译：提供了通过这些方法包装色谱柱和色谱柱的改进方法。所述方法包括将包装材料引入色谱柱，同时对包装材料进行超声波和施加压力以形成具有径向均匀性的填充柱。

8. 发明申请

US20140326055A1 CHROMATOGRAPHY OF METAL COMPLEXES 有权
标题翻译：金属复合物的色谱
公开(公告)号：US20140326055A1
公开(公告)日：2014-11-06
申请号：US14330745
申请日：2014-07-14
申请人： Urszula J. SLOMCZYNSKA , Bobby N. TRAWICK , Dennis P. RILEY , Arati NAIK
发明人： Urszula J. SLOMCZYNSKA , Bobby N. TRAWICK , Dennis P. RILEY , Arati NAIK
IPC分类号： G01N30/50 , G01N30/74
CPC分类号： G01N30/50 , B01D15/3828 , B01D15/3833 , B01D2015/3838 , G01N30/02 , G01N30/74 , G01N30/96 , G01N2030/027
摘要： A high performance liquid chromatography method to routinely and reproducibly detect and quantitate metal complexes is provided. The metal complexes used in the method of the invention can be different metal complexes, or they can be stereoisomers of the same metal complexes. The high performance liquid chromatography method of the present invention is suitable for the separation of diastereomers of the same metal complexes. Also provided is a chiral high performance liquid chromatography method to separate enantiomers of metal complexes. Superoxide dismutase mimetic compounds are also provided.
摘要翻译：提供了一种常规和可重复检测和定量金属络合物的高效液相色谱法。用于本发明方法的金属络合物可以是不同的金属络合物，或它们可以是相同金属络合物的立体异构体。本发明的高效液相色谱法适用于分离相同金属络合物的非对映异构体。还提供了用于分离金属络合物的对映异构体的手性高效液相色谱法。还提供了超氧化物歧化酶模拟化合物。

9. 发明授权

US08864999B2 Methods for regenerating acidic ion-exchange resins and reusing regenerants in such methods 有权
标题翻译：在这种方法中再生酸性离子交换树脂和再利用再生剂的方法
公开(公告)号：US08864999B2
公开(公告)日：2014-10-21
申请号：US12646327
申请日：2009-12-23
申请人： Timothy A. Brandvold , Stanley J. Frey , Vasken Abrahamian , Thomas Traynor
发明人： Timothy A. Brandvold , Stanley J. Frey , Vasken Abrahamian , Thomas Traynor
IPC分类号： B01D15/36 , G01N30/50 , B01J49/00 , B01D15/20 , C10L1/02 , B01D41/02 , G01N30/96
CPC分类号： B01J49/0069 , B01D15/20 , B01D15/203 , B01D15/362 , B01J49/06 , B01J49/53 , C10G2300/1011 , C10L1/02 , G01N30/50 , G01N30/96 , Y02P30/20
摘要： Methods for regenerating acidic ion-exchange resins and reusing regenerants in such methods are provided. A spent ion-exchange resin is contacted with an alcohol ion-exchange regenerant. The spent ion-exchange resin is thereafter contacted with an acidic ion-exchange regenerant to recharge the acidic ion-exchange resin to produce a regenerated acidic ion-exchange resin. Metal- and water-containing biomass-derived pyrolysis oil is then contacted with the regenerated acidic ion-exchange resin to produce low metal, water-containing biomass-derived pyrolysis oil. The regenerated acidic ion-exchange resin may be recycled. The spent alcohol and acid ion-exchange regenerants may be recovered and recycled.
摘要翻译：提供了在这种方法中再生酸性离子交换树脂和再利用再生剂的方法。将废弃的离子交换树脂与醇离子交换再生剂接触。然后将废弃的离子交换树脂与酸性离子交换再生剂接触以对酸性离子交换树脂再充电以产生再生的酸性离子交换树脂。然后将含金属和含水的生物质衍生的热解油与再生的酸性离子交换树脂接触以产生低金属，含水生物质的热解油。再生的酸性离子交换树脂可以再循环。废乙醇和酸离子交换再生剂可以回收和再循环。

10. 发明授权

US08818737B2 Methods, systems, algorithms and means for describing the possible conformations of actual and theoretical proteins and for evaluating actual and theoretical proteins with respect to folding, overall shape and structural motifs 有权
标题翻译：用于描述实际和理论蛋白质的可能构象以及评估折叠，整体形状和结构基序的实际和理论蛋白质的方法，系统，算法和手段
公开(公告)号：US08818737B2
公开(公告)日：2014-08-26
申请号：US12524572
申请日：2008-01-30
申请人： Jiaan Yang
发明人： Jiaan Yang
IPC分类号： G01N31/00 , G01N30/50 , G06G7/58
CPC分类号： G06F19/16 , G06F19/22
摘要： Methods, systems, algorithms and means for describing, analyzing and predicting protein folding motifs and other structures are provided. In one aspect, the Protein Folding Shape Code (PFSC) methods, systems, algorithms and means of the present invention apply generally to all of the categories of protein analysis and description, and are especially relevant to the geometric analysis and descriptions of proteins from their respective sequences or sequence portions. In a novel approach, the present inventions render analysis with respect to the alpha carbons of five-amino acid elements of a protein, utilizing available data to derive torsion angles and pitch distances, to thereby generate a series of overlapping analysis that can be expressed by a plurality of 27 vectors. Methods, systems and algorithms of the invention can be embodied in any computing device or portion thereof, and are adaptable to describe, analyze and predict the folding and other three-dimensional aspects of the structures of biomolecules such as nucleic acids, carbohydrates and glycoproteins. As yet another advantage, the present invention is adaptable as a tool for describing the conformations of many other organic molecules, and are thus especially suitable for use in the design of drugs, and the discovery and design of molecules which are to be adapted to interact with drugs.
摘要翻译：提供了用于描述，分析和预测蛋白质折叠图案和其他结构的方法，系统，算法和手段。在一个方面，本发明的蛋白质折叠形状代码（PFSC）方法，系统，算法和手段通常适用于蛋白质分析和描述的所有类别，并且与它们的蛋白质的几何分析和描述特别相关各序列或序列部分。在一种新颖的方法中，本发明使用可用数据导出扭转角和间距距离，对蛋白质的五个氨基酸元素的α碳进行分析，从而产生一系列重叠分析，可以由多个27个向量。本发明的方法，系统和算法可以体现在任何计算设备或其部分中，并且适于描述，分析和预测生物分子的结构的折叠和其他三维方面，例如核酸，碳水化合物和糖蛋白。作为另一个优点，本发明可适用于描述许多其它有机分子的构象的工具，因此特别适用于药物的设计，以及适于相互作用的分子的发现和设计用药。

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