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1. 发明授权

US4225381A Method for removing odor from fluid 失效
标题翻译：从流体中除去气味的方法
公开(公告)号：US4225381A
公开(公告)日：1980-09-30
申请号：US968637
申请日：1978-12-12
申请人： Hisao Ishikawa , Yukio Kita , Kouki Horikoshi
发明人： Hisao Ishikawa , Yukio Kita , Kouki Horikoshi
IPC分类号： C02F3/34 , D21C11/08 , D21C11/10
CPC分类号： C12R1/66 , C02F3/345 , C12R1/745 , C12R1/79 , C12R1/80 , C12R1/885 , D21C11/08 , D21C11/10
摘要： A method for removing malodorous sulfur compounds from a fluid containing such compounds. In this method, fungi belonging to genera Cephalosporium, Paecilomyces, Penicillium, Aspergillus, Trichoderma and Mucor can be used for effectively removing malodorous sulfur compounds from a fluid.
摘要翻译：从含有这些化合物的流体中除去恶臭硫化合物的方法。在这种方法中，可以使用属于头孢菌属，拟青霉属，青霉菌，曲霉属，木霉属和毛霉属的真菌，以有效地从流体中除去恶臭的硫化合物。

2. 发明授权

US5362640A Ornithine carbamoyl transferase gene and utilization of the DNA 失效
标题翻译：鸟氨酸氨基甲酰转移酶基因和DNA的利用
公开(公告)号：US5362640A
公开(公告)日：1994-11-08
申请号：US27986
申请日：1993-03-08
申请人： Akira Tsukamoto , Mieko Matsufuji , Yukio Kita
发明人： Akira Tsukamoto , Mieko Matsufuji , Yukio Kita
IPC分类号： C12N1/15 , C12N9/02 , C12N9/08 , C12N9/10 , C12N15/53 , C12N15/54 , C12N15/80 , C12N15/00 , C07H17/00 , C12N1/14 , C12P21/04
CPC分类号： C12N9/0071 , C12N15/80 , C12N9/0065 , C12N9/0069 , C12N9/1018 , C12Y114/13007 , C07K2319/02
摘要： This invention relates to a novel ornithine carbamoyl transferase (OCTase) gene, a recombinant DNA containing the OCTase DNA, a transformation system for a basidiomycete, and production of useful proteins using the system.More particularly, this invention relates to the OCTase gene of Coriolus hirsutus to afford an efficient host-vector system in basidiomycetes (particularly a white rot fungus such as Coriolus hirsutus IFO 4917) for the preparation of useful proteins. OCTase is the enzyme to transform ornithine to citrulline in arginine biosynthesis in organisms. The present invention provides the OCTase genes of C. hirsutus, the useful Arg.sup.- auxotrophic mutant of C. hirsutus deficient in the OCTase gene, the efficient condition for the preparation of protoplasts and the transformation of the mutant with the cloned OCTase gene and the recombinant DNA's including a promoter, a signal peptide-coding DNA and a protein-coding DNA, the construction of the novel host-vector system of C. hirsutus, and the new method of a large scale preparation of useful proteins using this novel host-vector system with a new recombinant DNA technique.Furthermore, this invention provides a highly efficient method to produce a useful protein such as lignin peroxidase which is difficult to produce by the conventional method.
摘要翻译：本发明涉及新型鸟氨酸氨基甲酰转移酶（OCTase）基因，含有OCTase DNA的重组DNA，担子菌的转化系统，以及使用该系统生产有用的蛋白质。更具体地说，本发明涉及毛地科氏菌的OCTase基因，以提供担当菌纲（尤其是白腐真菌例如Coriolus hirsutus IFO 4917）中有效的宿主 - 载体系统，用于制备有用的蛋白质。 OCTase是在生物体中精氨酸生物合成中将鸟氨酸转化为瓜氨酸的酶。本发明提供了陆地多毛菌素的OCTase基因，OCTase基因缺陷的陆地棉属的有用的Arg-营养缺陷突变体，用于制备原生质体的有效条件以及用克隆的OCTase基因和重组体转化突变体包括启动子，信号肽编码DNA和蛋白质编码DNA的DNA，构建高山羊新宿主 - 载体系统，以及使用该新型宿主载体大量制备有用蛋白质的新方法系统与新的重组DNA技术。此外，本发明提供了生产有用的蛋白质如木质素过氧化物酶的高效方法，其难以通过常规方法生产。

3. 发明授权

US06075138A Transcriptional regulatory DNA sequence elements and signal peptide sequence of the Coriolus hirsutus phenoloxidase gene, and plasmid vectors and transformants utilizing such sequences 失效
标题翻译：柯氏杆菌酚氧化酶基因的转录调控DNA序列元件和信号肽序列，以及利用这种序列的质粒载体和转化体
公开(公告)号：US06075138A
公开(公告)日：2000-06-13
申请号：US999958
申请日：1993-01-15
申请人： Yasushi Kojima , Yukio Kita , Yukiko Tsukuda
发明人： Yasushi Kojima , Yukio Kita , Yukiko Tsukuda
IPC分类号： C12N9/02 , C12N15/53 , C12N15/62 , C12N15/80 , C12N15/81 , C12N15/67
CPC分类号： C12N9/0073 , C12N15/625 , C12N15/80 , C12N15/81 , C12Y114/13007 , C07K2319/00 , C07K2319/02 , C07K2319/036
摘要： This invention relates to a novel DNA or a derivative thereof concerning the expression and secretion of a phenoloxidase gene originating in a basidiomycete (particularly a white rot fungus such as, for example Coriolus hirsutus IFO 4917) possessing the ability to produce and secrete a phenoloxidase, a novel organism containing the DNA and a protein-coding DNA, and a method for the production of proteins by the use of the novel organisms. The aforementioned "DNA concerning expression" generally refers as to a region existent on the upstream side of a structural gene and necessary for the initiation of transcription (promoter) and to a region existent on the downstream side thereof and necessary for the termination of the transcription (poly A signal or terminator), for example. The "DNA concerning secretion" generally refers to a DNA which codes a signal peptide. The aforementioned "DNA derivative" includes a still longer derivative accompanying a flanking sequence and variants associated with fragment of the DNA mentioned above, substitution of a nucleotide of the aforementioned DNA, insertion of a nucleotide, deletion of nucleotide, inversion of a nucleotide sequence, and other forms of mutation. These derivatives are obtained naturally, synthetically, or semisynthetically.
摘要翻译：本发明涉及一种新型的DNA或其衍生物，其涉及具有产生和分泌酚氧化酶的能力的担当菌（特别是白腐菌例如Coriolus hirsutus IFO 4917）的酚氧化酶基因的表达和分泌，含有DNA和蛋白质编码DNA的新型生物体，以及通过使用新生物体生产蛋白质的方法。上述“关于表达的DNA”通常是指结构基因上游侧存在的区域，并且是启动转录（启动子）所必需的，并且在其下游侧存在并且终止转录所必需的区域（poly A信号或终止子）。 “关于分泌的DNA”通常是指编码信号肽的DNA。上述“DNA衍生物”包括伴随侧翼序列的更长的衍生物和与上述DNA的片段相关的变体，上述DNA的核苷酸的取代，核苷酸的插入，核苷酸的缺失，核苷酸序列的翻转，和其他形式的突变。这些衍生物是天然的，合成的或半合成的。

4. 发明授权

US5916795A Thermostable xylanase 失效
标题翻译：热稳定的木聚糖酶
公开(公告)号：US5916795A
公开(公告)日：1999-06-29
申请号：US963500
申请日：1997-11-03
申请人： Nobuyuki Fukunaga , Yuji Iwasaki , Satoko Kono , Yukio Kita , Yoshiya Izumi
发明人： Nobuyuki Fukunaga , Yuji Iwasaki , Satoko Kono , Yukio Kita , Yoshiya Izumi
IPC分类号： C12N15/09 , C07H21/04 , C12N1/20 , C12N1/21 , C12N9/24 , C12N9/42 , C12R1/07 , C12S3/08 , D21C5/00 , D21C9/10
CPC分类号： C12Y302/01032 , C12N9/248 , C12Y302/01008 , D21C5/005 , Y10S435/832 , Y10S435/835
摘要： The invention relates to a thermostable xylanase selected from xylanase XP1 having a molecular weight of about 22,500, an isoelectric point at around 8.1 and an optimum temperature for reaction 70.degree. C. or xylanase XP2 having a molecular weight of about 32,000, an isoelectric point at around 8.5 and an optimum temperature for reaction of 80.degree. C., a gene encoding for the thermostable xylanase, a method for producing the xylanase, applications of the xylanase, a bleaching agent containing the xylanase as an active ingredient, a method for bleaching pulp by using the bleaching agent and Bacillus sp. 2113 and Bacillus sp. 208 both having an ability to produce a thermostable xylanase.
摘要翻译：本发明涉及一种选自分子量约22,500的木聚糖酶XP1，约8.1的等电点和70℃的反应的最适温度或分子量为约32,000的木聚糖酶XP2的热稳定性木聚糖酶，等电点 8.5左右，80℃反应的最佳温度，编码热稳定性木聚糖酶的基因，木聚糖酶的生产方法，木聚糖酶的应用，含有木聚糖酶作为活性成分的漂白剂，漂白纸浆的方法通过使用漂白剂和芽孢杆菌 2113和Bacillus sp。 208都具有产生热稳定性木聚糖酶的能力。

5. 发明授权

US5736384A Thermostable xylanase 失效
公开(公告)号：US5736384A
公开(公告)日：1998-04-07
申请号：US575964
申请日：1995-12-20
申请人： Nobuyuki Fukunaga , Yuji Iwasaki , Satoko Kono , Yukio Kita , Yoshiya Izumi
发明人： Nobuyuki Fukunaga , Yuji Iwasaki , Satoko Kono , Yukio Kita , Yoshiya Izumi
IPC分类号： C12N15/09 , C07H21/04 , C12N1/20 , C12N1/21 , C12N9/24 , C12N9/42 , C12R1/07 , C12S3/08 , D21C5/00 , D21C9/10 , C12S3/00
CPC分类号： C12Y302/01032 , C12N9/248 , C12Y302/01008 , D21C5/005 , Y10S435/832 , Y10S435/835
摘要： The invention relates to a thermostable xylanase selected from xylanase XP1 having a molecular weight of about 22,500, an isoelectric point at around 8.1 and an optimum temperature for reaction of 70.degree. C. or xylanase XP2 having a molecular weight of about 32,000, an isoelectric point at around 8.5 and an optimum temperature for reaction of 80.degree. C., a gene encoding for the thermostable xylanase, a method for producing the xylanase, applications of the xylanase, a bleaching agent containing the xylanase as an active ingredient, a method for bleaching pulp by using the bleaching agent and Bacillus sp. 2113 and Bacillus sp. 208 both having an ability to produce a thermostable xylanase.

6. 发明授权

US5616290A Gas-liquid contacting apparatus 失效
公开(公告)号：US5616290A
公开(公告)日：1997-04-01
申请号：US650710
申请日：1996-05-20
申请人： Makiichi Ishihara , Takakazu Sunada , Shigeo Hasegawa , Naohiko Ukawa , Toru Takashina , Yukio Kita , Kouichiro Iwashita , Kousuke Yamashita , Junji Ozaki , Kaname Kaneshige
发明人： Makiichi Ishihara , Takakazu Sunada , Shigeo Hasegawa , Naohiko Ukawa , Toru Takashina , Yukio Kita , Kouichiro Iwashita , Kousuke Yamashita , Junji Ozaki , Kaname Kaneshige
IPC分类号： B01D53/18 , B01D53/50 , B01J10/00 , B01J19/26 , B05B15/06 , B05B1/00
CPC分类号： B01J19/0066 , B01D53/185 , B01D53/504 , B01J10/002 , B01J19/26 , B05B15/069 , Y10S261/39 , Y10S261/75
摘要： A gas-liquid contacting apparatus in which spray nozzles for spraying slurry solution upward are arranged in a tower body through which gas passes, wherein a liquid inlet portion of the spray nozzle is of a bell shape having a radius of curvature of 0.2 times or more of the diameter of a liquid discharge port of the spray nozzle, and a ceramic nozzle support structure comprises: (A) a ceramic nozzle having a ridge portion on the outer peripheral surface of a nozzle body; (B) a tightening flange including a flange body having an aperture larger than the outside shape of the nozzle body and a central cylindrical portion extending substantially concentrically with the nozzle body from lower surface of the flange body, the tightening flange having through holes in the wall of central cylindrical portion thereof; (C) a support nozzle consisting of a cylindrical body having an inside diameter larger than the central cylindrical portion of tightening flange and connected to the tightening flange by bolting via the flange body at the upper portion of the cylindrical body; and (D) an elastomeric resin filled in a space between the nozzle and the support nozzle so that the outside and inside of the central cylindrical portion of tightening flange are integrated via the through holes.

7. 发明授权

US5888802A Thermostable xylanase 失效
标题翻译：热稳定的木聚糖酶
公开(公告)号：US5888802A
公开(公告)日：1999-03-30
申请号：US963497
申请日：1997-11-03
申请人： Nobuyuki Fukunaga , Yuji Iwasaki , Satoko Kono , Yukio Kita , Yoshiya Izumi
发明人： Nobuyuki Fukunaga , Yuji Iwasaki , Satoko Kono , Yukio Kita , Yoshiya Izumi
IPC分类号： C12N15/09 , C07H21/04 , C12N1/20 , C12N1/21 , C12N9/24 , C12N9/42 , C12R1/07 , C12S3/08 , D21C5/00 , D21C9/10 , C12S3/00
CPC分类号： C12Y302/01032 , C12N9/248 , C12Y302/01008 , D21C5/005 , Y10S435/832 , Y10S435/835
摘要： The invention relates to a thermostable xylanase selected from xylanase XP1 having a molecular weight of about 22,500, an isoelectric point at around 8.1 and an optimum temperature for reaction of 70.degree. C. or xylanase XP2 having a molecular weight of about 32,000, an isoelectric point at around 8.5 and an optimum temperature for reaction of 80.degree. C., a gene encoding for the thermostable xylanase, a method for producing the xylanase, applications of the xylanase, a bleaching agent containing the xylanase as an active ingredient, a method for bleaching pulp by using the bleaching agent and Bacillus sp. 2113 and Bacillus sp. 208 both having an ability to produce a thermostable xylanase.
摘要翻译：本发明涉及一种选自分子量约22,500的木聚糖酶XP1，约8.1的等电点和70℃的反应的最适温度或分子量为约32,000的木聚糖酶XP2的热稳定性木聚糖酶，等电点约8.5，最适温度为80℃，编码热稳定性木聚糖酶的基因，木聚糖酶的生产方法，木聚糖酶的应用，含有木聚糖酶作为活性成分的漂白剂，漂白方法通过使用漂白剂和芽孢杆菌 2113和Bacillus sp。 208都具有产生热稳定性木聚糖酶的能力。

8. 发明授权

US5605655A Gas-liquid contacting apparatus 失效
标题翻译：气液接触装置
公开(公告)号：US5605655A
公开(公告)日：1997-02-25
申请号：US413722
申请日：1995-03-31
申请人： Makiichi Ishihara , Takakazu Sunada , Shigeo Hasegawa , Naohiko Ukawa , Toru Takashina , Yukio Kita , Kouichiro Iwashita , Kousuke Yamashita , Junji Ozaki , Kaname Kaneshige
发明人： Makiichi Ishihara , Takakazu Sunada , Shigeo Hasegawa , Naohiko Ukawa , Toru Takashina , Yukio Kita , Kouichiro Iwashita , Kousuke Yamashita , Junji Ozaki , Kaname Kaneshige
IPC分类号： B01D53/18 , B01D53/50 , B01J10/00 , B01J19/26 , B05B15/06 , B01D53/34
CPC分类号： B01J19/0066 , B01D53/185 , B01D53/504 , B01J10/002 , B01J19/26 , B05B15/069 , Y10S261/39 , Y10S261/75
摘要： A gas-liquid contacting apparatus in which spray nozzles for spraying slurry solution upward are arranged in a tower body through which gas passes, wherein a liquid inlet portion of the spray nozzle is of a bell shape having a radius of curvature of 0.2 times or more of the diameter of a liquid discharge port of the spray nozzle, and a ceramic nozzle support structure comprises: (A) a ceramic nozzle having a ridge portion on the outer peripheral surface of a nozzle body; (B) a tightening flange including a flange body having an aperture larger than the outside shape of the nozzle body and a central cylindrical portion extending substantially concentrically with the nozzle body from lower surface of the flange body, the tightening flange having through holes in the wall of central cylindrical portion thereof; (C) a support nozzle consisting of a cylindrical body having an inside diameter larger than the central cylindrical portion of tightening flange and connected to the tightening flange by bolting via the flange body at the upper portion of the cylindrical body; and (D) an elastomeric resin filled in a space between the nozzle and the support nozzle so that the outside and inside of the central cylindrical portion of tightening flange are integrated via the through holes.
摘要翻译：一种气液接触装置，其中向上喷射浆液的喷嘴布置在气体通过的塔体中，其中喷嘴的液体入口部分具有曲率半径为0.2倍或更大的钟形形状所述喷嘴支撑结构包括：（A）在喷嘴体的外周面上具有脊部的陶瓷喷嘴，（B）包括具有比所述喷嘴体的外形大的孔的凸缘体的紧固凸缘和从所述凸缘体的下表面与所述喷嘴体大致同心地延伸的中心圆筒部，所述紧固凸缘在所述凸缘主体中具有通孔其中心圆柱形部分的壁; （C）支撑喷嘴，其由具有大于紧固凸缘的中心圆柱形部分的内径的圆柱形主体组成，并通过在圆柱体的上部处的凸缘体进行螺栓连接而与紧固凸缘连接; 和（D）填充在喷嘴和支撑喷嘴之间的空间中的弹性体树脂，使得紧固凸缘的中心圆柱形部分的外部和内部通过通孔一体化。

9. 发明授权

US4514501A Method for cultivation of microorganism 失效
标题翻译：微生物培养方法
公开(公告)号：US4514501A
公开(公告)日：1985-04-30
申请号：US348194
申请日：1982-02-12
申请人： Yukio Kita , Kazuo Koide , Kouki Horiko
发明人： Yukio Kita , Kazuo Koide , Kouki Horiko
IPC分类号： C12N1/20 , C12N1/22 , C12N1/24 , C12R1/06 , C12R1/07 , C02F3/34 , D21C11/00
CPC分类号： C12N1/22 , C12N1/24 , Y10S210/928 , Y10S435/804 , Y10S435/83 , Y10S435/832 , Y10S435/84 , Y10S435/843
摘要： A method for cultivating, under alkaline conditions, microorganisms in a culture medium containing, as a carbon source, the extracted liquor or spent liquor derived from alkaline pulping is presented. In this cultivation, organic acids contained in the extracted liquor or spent liquor can be effectively utilized. Typical microorganisms cultured are bacteria belonging to the genera Bacillus, Arthrobacter, Corynebacterium and Brevibacterium.
摘要翻译：在碱性条件下培养含有源自碱性制浆的提取液或废液作为碳源的培养基中的微生物的方法。在这种培养中，可以有效地利用提取的液体或废液中所含的有机酸。培养的典型微生物是属于芽孢杆菌属，节杆菌属，棒状杆菌属和短杆菌属的细菌。

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