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1. 发明授权

US5492818A Method of producing L-glutamic acid by fermentation 失效
标题翻译：通过发酵生产L-谷氨酸的方法
公开(公告)号：US5492818A
公开(公告)日：1996-02-20
申请号：US354014
申请日：1994-12-05
申请人： Hidetsugu Nakazawa , Hiroki Kawashima , Inao Oyama , Keiji Ishii , Yoshio Kawahara
发明人： Hidetsugu Nakazawa , Hiroki Kawashima , Inao Oyama , Keiji Ishii , Yoshio Kawahara
IPC分类号： C12P13/14 , C12R1/13 , C12R1/15 , C12P13/18
CPC分类号： C12P13/14 , Y10S435/843
摘要： The present invention provides a method of producing L-glutamic acid by fermentation, comprising the steps ofculturing a mutant of an L-glutamic acid-producing microorganism of the genus Brevibacterium or Corynebacterium which has lower .alpha.-ketoglutaric acid dehydrogenase activity compared with the wild strains from which said mutant is derived, in a liquid nutrient culture medium containing biotin at a concentration of 10 to 1000 .mu.g/l without adding a biotin activity-suppressing substance thereto;producing and accumulating L-glutamic acid in the culture solution; andrecovering L-glutamic acid from said culture solution.According to the method of the present invention, it is possible to industrially produce L-glutamic acid by fermentation in a more economical and efficient manner.
摘要翻译：本发明提供通过发酵生产L-谷氨酸的方法，包括以下步骤：与野生型相比，培养具有较低α-酮戊二酸脱氢酶活性的短杆菌属或棒状杆菌属的L-谷氨酸生产微生物的突变体在不含生物素活性抑制物质的情况下，在含有浓度为10〜1000μg/ l的生物素的液体营养培养基中，所述突变体得自其中的菌株; 在培养液中产生和积累L-谷氨酸; 从所述培养液中回收L-谷氨酸。根据本发明的方法，可以以更经济和有效的方式通过发酵工业生产L-谷氨酸。

2. 发明授权

US06803062B2 Process for producing hydrolyzed protein 有权
标题翻译：生产水解蛋白的方法
公开(公告)号：US06803062B2
公开(公告)日：2004-10-12
申请号：US10183686
申请日：2002-06-28
申请人： Ko Yamamoto , Junichiro Kojima , Toshimasa Ishii , Inao Oyama , Mitsuyoshi Seki , Hidetsugu Nakazawa
发明人： Ko Yamamoto , Junichiro Kojima , Toshimasa Ishii , Inao Oyama , Mitsuyoshi Seki , Hidetsugu Nakazawa
IPC分类号： A23L100
CPC分类号： C12P21/06 , A23J3/34 , A23J3/346 , A23L27/21
摘要： A hydrolyzed protein which can be used as seasonings or the like is produced by heating and maintaining an aqueous dispersion of a protein-containing starting material, while under an acidic condition, with a plate-type heat exchanger using a liquid as a heat medium to make the aqueous dispersion substantially aseptic and then subjecting the resulting aqueous dispersion with an action of a proteolytic enzyme.
摘要翻译：可以用作调味料等的水解蛋白质通过在使用液体作为热介质的板式热交换器的酸性条件下加热并保持含蛋白质原料的水分散体来制备，使水分散体基本上是无菌的，然后使所得的水分散体用蛋白水解酶的作用进行。

3. 发明授权

US06858405B1 Process for producing protein hydrolyzate 有权
标题翻译：生产蛋白水解产物的方法
公开(公告)号：US06858405B1
公开(公告)日：2005-02-22
申请号：US09674280
申请日：1999-04-23
申请人： Michinobu Nakamura , Mitsuyoshi Seki , Miyoko Nawata , Hidetsugu Nakazawa , Hideki Okamura
发明人： Michinobu Nakamura , Mitsuyoshi Seki , Miyoko Nawata , Hidetsugu Nakazawa , Hideki Okamura
IPC分类号： A23J3/34 , A23L27/50 , C12P1/02 , C12P21/00 , C12P21/06 , C12N1/14
CPC分类号： C12P21/00 , A23J3/346 , A23L27/50 , C12P1/02 , C12R1/69 , Y10S435/911 , Y10S435/913 , Y10S435/918
摘要： The present invention relates to a method which can prevent browning of hydrolyzed protein obtained by enzymatic hydrolysis of vegetable protein material.A vegetable protein material containing saccharides is mixed with the fungal culture and is subjected to enzymatic hydrolysis in a liquid reaction system. The reaction is conducted first at a temperature ranging from 15° C. to 39° C. with aeration and agitation, and then, after stopping the aeration, the reaction is conducted and completed at a temperature ranging from 40° C. to 60° C.
摘要翻译：本发明涉及能够防止由植物蛋白质材料的酶水解而得到的水解蛋白质的褐变的方法。将含有糖的植物蛋白材料与真菌培养物混合，并在液体反应体系中进行酶水解。首先在通风和搅拌下在15℃至39℃的温度下进行反应，然后在通气停止后，在40℃至60℃的温度范围内进行反应 C。

4. 发明授权

US3930948A Enzymatic method for producing aromatic ethylamines 失效
标题翻译：用于生产芳族乙酰胺的酶学方法
公开(公告)号：US3930948A
公开(公告)日：1976-01-06
申请号：US492906
申请日：1974-07-29
申请人： Konosuke Sano , Keizo Matsuda , Hidetsugu Nakazawa , Koji Mitsugi
发明人： Konosuke Sano , Keizo Matsuda , Hidetsugu Nakazawa , Koji Mitsugi
IPC分类号： C12P13/00 , C07C209/68 , C07D209/16 , C12N9/88 , C12P13/22 , C12D13/00
CPC分类号： C07C209/68 , C07D209/16 , C12N9/88 , Y10S435/859
摘要： A decarboxylase in cells of Micrococcus strains converts tryptophan, phenylalanine, and substitution products thereof to the corresponding phenyl- and indolylethylamines. The enzyme can be recovered in pure state from the microorganisms, but cells and cell material are effective enzyme sources in the decarboxylation reaction.

5. 发明申请

US20090258400A1 Mutant Tyrosine Repressor, a Gene Encoding the Same, and a Method for Producing L-Dopa 失效
标题翻译：突变型酪氨酸阻抑素，编码其的基因以及产生L-多巴的方法
公开(公告)号：US20090258400A1
公开(公告)日：2009-10-15
申请号：US11855256
申请日：2007-09-14
申请人： Hidehiko Kumagai , Hideyuki Suzuki , Takane Katayama , Miyoko Nawata , Hidetsugu Nakazawa
发明人： Hidehiko Kumagai , Hideyuki Suzuki , Takane Katayama , Miyoko Nawata , Hidetsugu Nakazawa
IPC分类号： C12P13/22 , C07H21/04 , C12N1/21
CPC分类号： C07K14/27
摘要： A mutant tyrosine repressor that does not require tyrosine to induce expression of tyrosine phenol-lyase gene is obtained by introducing a mutation into a tyrosine repressor. A microorganism which is able to express large amounts of tyrosine phenol-lyase is obtained by introducing the mutant tyrosine repressor into the microorganism. The microorganism is useful for producing L-DOPA.
摘要翻译：通过将突变引入到酪氨酸阻遏物中，获得不需要酪氨酸诱导酪氨酸苯酚裂合酶基因表达的突变型酪氨酸阻遏物。通过将突变型酪氨酸阻遏物引入微生物获得能够表达大量酪氨酸酚解酶的微生物。该微生物可用于生产L-DOPA。

6. 发明授权

US07365161B2 Mutant tyrosine repressor which does not require tyrosine to induce expression of the tyrosine phenol-lyase gene 失效
标题翻译：不需要酪氨酸诱导酪氨酸苯酚裂合酶基因表达的突变型酪氨酸阻遏物
公开(公告)号：US07365161B2
公开(公告)日：2008-04-29
申请号：US11091914
申请日：2005-03-29
申请人： Hidehiko Kumagai , Hideyuki Suzuki , Takane Katayama , Miyoko Nawata , Hidetsugu Nakazawa
发明人： Hidehiko Kumagai , Hideyuki Suzuki , Takane Katayama , Miyoko Nawata , Hidetsugu Nakazawa
IPC分类号： C07K1/00
CPC分类号： C07K14/27
摘要： A mutant tyrosine repressor that does not require tyrosine to induce expression of tyrosine phenol-lyase gene is obtained by introducing a mutation into a tyrosine repressor (tyrR), isolated from Pantoea agglomerans, or a tyrosine repressor (tyrR) which is encoded by SEQ ID NO. 1 and its derivative. A microorganism which is able to express large amounts of tyrosine phenol-lyase is obtained by introducing the mutant tyrosine repressor into the microorganism. The microorganism is useful for producing L-DOPA.
摘要翻译：通过将突变引入由成团泛菌或从SEQ ID NO：1编码的酪氨酸阻遏物（tyrR）分离的酪氨酸阻遏物（tyrR）中，获得不需要酪氨酸诱导酪氨酸酚解酶基因表达的突变型酪氨酸阻遏物没有。 1及其衍生物。通过将突变型酪氨酸阻遏物引入微生物获得能够表达大量酪氨酸酚解酶的微生物。该微生物可用于生产L-DOPA。

7. 发明授权

US5573670A Method for treatment of waste water by activated sludge process 失效
标题翻译：活性污泥法处理废水的方法
公开(公告)号：US5573670A
公开(公告)日：1996-11-12
申请号：US361455
申请日：1994-12-21
申请人： Yoshimi Nagasaki , Hidetsugu Nakazawa
发明人： Yoshimi Nagasaki , Hidetsugu Nakazawa
IPC分类号： C02F1/78 , C02F3/12 , C02F3/26
CPC分类号： C02F3/121 , C02F3/26 , Y02W10/15
摘要： Waste water is treated by an activated sludge process, the equipment for which is provided with an aeration tank unit comprised of three tanks of a first tank, a second tank and a third tank, said waste water and microorganisms being fed into the first tank while the ratio of aeration in the first tank ranges from 0.5 to 0.8 by volume/minute relative to the amount of liquid in the first tank, the ratio of aeration in the second tank ranges from 0. 1 to 0.4 by volume/minute relative to the amount of liquid in the said second tank and the ratio of aeration in the third tank ranges from 0.1 to 0.3 by volume/minute relative to the amount of liquid in the third tank, the improvement which comprises carrying out said treatment by passing air containing ozone in an amount of from 0.01 to 0.16 wt. % relative to the amount of oxygen during the aeration into the first tank.
摘要翻译：废水通过活性污泥法处理，其设备设置有由第一罐，第二罐和第三罐的三个罐组成的曝气池单元，所述废水和微生物进料到第一罐中，相对于第一罐中的液体量，第一罐中的曝气比例为0.5至0.8体积/分钟，第二罐中的曝气比例相对于第一罐的通量为0.1至0.4体积/分钟所述第二罐中的液体量和第三罐中的曝气量相对于第三罐中的液体量为0.1至0.3体积/分钟，其改进包括通过使含臭氧的空气进行所述处理其量为0.01至0.16wt。相对于通入第一罐的氧气量的百分比。

8. 发明授权

US4071405A Method of producing sulfur containing L-amino acids 失效
标题翻译：生产含L-氨基酸的硫的方法
公开(公告)号：US4071405A
公开(公告)日：1978-01-31
申请号：US696398
申请日：1976-06-15
申请人： Kenji Soda , Hidehiko Tanaka , Hidetsugu Nakazawa , Koji Mitsugi
发明人： Kenji Soda , Hidehiko Tanaka , Hidetsugu Nakazawa , Koji Mitsugi
IPC分类号： C12P13/04 , C12P13/12 , C12R1/38 , C12D13/06
CPC分类号： C12P13/12 , C12P13/04 , Y10S435/842 , Y10S435/849 , Y10S435/874
摘要： Method for the production of substituted sulfur containing L-amino acids by contacting .beta.- or .gamma.-substituted amino acids in an aqueous medium containing methioninase together with the precursor of the substituent.
摘要翻译：通过使含有蛋氨酸酶的水性介质中的β-或γ-取代的氨基酸与取代基的前体接触来生产含有L-氨基酸的取代的硫的方法。

9. 发明授权

US09204660B2 Feed additive composition for ruminants and method of producing the same 有权
标题翻译：反刍动物饲料添加剂组合物及其制备方法
公开(公告)号：US09204660B2
公开(公告)日：2015-12-08
申请号：US12896264
申请日：2010-10-01
申请人： Hidetsugu Nakazawa , Hiroyuki Sato , Yuki Miyazawa , Susumu Shibahara , Sachiko Oka , Yumi Goto
发明人： Hidetsugu Nakazawa , Hiroyuki Sato , Yuki Miyazawa , Susumu Shibahara , Sachiko Oka , Yumi Goto
IPC分类号： A23K1/18 , A23K3/00 , A23L1/00 , A23K1/16 , A23K1/00
CPC分类号： A23K1/1634 , A23K20/142 , A23K20/158 , A23K40/35 , A23K50/10
摘要： A feed additive composition includes a protective agent, lecithin in an amount of 0.05 to 6% by weight relative to a total weight of the composition, a basic amino acid in an amount of at least 40% by weight and less than 65% by weight relative to the total weight of the composition, and water. A method of producing a feed additive composition includes preparing a molten mixture of at least one protective agent, lecithin and at least one basic amino acid, and solidifying the molten mixture by immersing the molten mixture in water or an aqueous liquid. The protective agent includes hydrogenated vegetable oils and/or hydrogenated animal oils having melting points of greater than 50° C. and less than 90° C.
摘要翻译：饲料添加剂组合物包含相对于组合物总重量为0.05至6重量％的保护剂卵磷脂，至少40重量％且小于65重量％的碱性氨基酸相对于组合物的总重量和水。制备饲料添加剂组合物的方法包括制备至少一种保护剂，卵磷脂和至少一种碱性氨基酸的熔融混合物，并将熔融混合物浸入水或含水液体中来固化熔融混合物。保护剂包括熔点大于50℃且小于90℃的氢化植物油和/或氢化动物油。

10. 发明授权

US08399038B2 Method for producing dried microbial cells 失效
标题翻译：干燥微生物细胞的制备方法
公开(公告)号：US08399038B2
公开(公告)日：2013-03-19
申请号：US12147677
申请日：2008-06-27
申请人： Hidetsugu Nakazawa , Togo Hotta , Hiroyuki Sato
发明人： Hidetsugu Nakazawa , Togo Hotta , Hiroyuki Sato
IPC分类号： A47J39/00 , A23C9/12 , A23L1/18 , A23B4/03
CPC分类号： C12N1/005 , A23K10/16 , A23K40/10 , A23K40/20 , A23K40/25 , A23K50/10 , C12P13/08
摘要： An inexpensive heat treatment method for producing dried microbial cells is provided, wherein the method does not result in lowering the quality of the microbial cells or causing pulverization. The method includes a step of heating microbial cells to between 200 to 450° C. for 1 to 30 seconds.
摘要翻译：提供了用于生产干燥的微生物细胞的廉价的热处理方法，其中该方法不会导致微生物细胞的质量降低或导致粉碎。该方法包括将微生物细胞加热至200-450℃持续1至30秒的步骤。

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