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    • 6. 发明授权
    • Method for the production of L-threonine by fermentation, using mutated
DNA encoding aspartokinase III
    • 通过发酵生产L-苏氨酸的方法,使用编码天冬氨酸激酶III的突变型DNA
    • US5661012A
    • 1997-08-26
    • US256136
    • 1994-07-01
    • Konosuke SanoHiroyuki KojimaYuri Ogawa
    • Konosuke SanoHiroyuki KojimaYuri Ogawa
    • C12N1/21C12N9/12C12N15/54C12N15/63C12P13/08C12R1/19
    • C12N9/1217C12N9/12C12P13/08
    • A DNA containing a gene which codes for aspartokinase III originating in Escherichia bacteria and which has in the coding region a mutation which releases the feedback inhibition by lysine on the aspartokinase III, a recombinant DNA wherein the DNA is linked to a vector DNA capable of autonomous replication in Escherichia bacteria, a microorganism belonging to the genus Escherichia which has been transformed by the introduction of the above mentioned recombinant DNA into the cells thereof, and a method for the production of L-threonine which comprises culturing the microorganism in a fermentation medium, producing and accumulating L-threonine in the culture medium, and collecting the L-threonine from said-culture medium. Escherichia bacteria-derived AK III genes are obtained which sufficiently release the feedback inhibition due to lysine. Introduction of these genes into threonine-producing bacteria results in new threonine-producing bacteria which are much improved in their threonine production over those of the prior art. Use of these threonine-producing bacteria provides a much more excellent method for the production of L-threonine by fermentation than the conventional methods.
    • PCT No.PCT / JP93 / 01640 Sec。 371日期:1994年7月1日 102(e)日期1994年7月1日PCT 1993年11月10日PCT公布。 出版物WO94 / 11517 日期1994年5月26日包含编码源自大肠杆菌细菌的天冬氨酸激酶III的基因的DNA,其编码区具有在天冬氨酸激酶III上释放赖氨酸的反馈抑制的突变,其是DNA与载体连接的重组DNA 能够在大肠杆菌中自主复制的DNA,通过将上述重组DNA导入其细胞而转化的埃希氏杆菌属的微生物,以及L-苏氨酸的制备方法,其特征在于, 发酵培养基,在培养基中产生和积累L-苏氨酸,并从所述培养基中收集L-苏氨酸。 获得大肠杆菌细菌来源的AK III基因,其充分释放由赖氨酸引起的反馈抑制。 将这些基因引入产生苏氨酸的细菌导致新的苏氨酸生产细菌,其苏氨酸生产比现有技术的产生大大改善。 使用这些苏氨酸生产菌比传统方法提供了通过发酵生产L-苏氨酸的更优异的方法。