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    • 9. 发明授权
    • Process for electro-dialysis
    • 电透析方法
    • US4160713A
    • 1979-07-10
    • US873680
    • 1978-01-30
    • Harumi MatsuzakiSankichi TakahashiOsamu Kuroda
    • Harumi MatsuzakiSankichi TakahashiOsamu Kuroda
    • B01D65/06B01D13/02
    • B01D61/44B01D2311/165B01D2311/243B01D2311/2661
    • In a process for electro-dialysis which comprises passing an electrolyte through dilution chambers and concentration chambers formed between a large number of alternately counterposed anion exchange membranes and cation exchange membranes, and applying an electric potential to a pair of electrodes provided at both ends of a plurality of these chambers, thereby migrating ions in the electrolyte through the membranes and effecting dilution or concentration of the electrolyte, such a volumic rate of air bubbles is introduced to the lower parts of the dilution chambers as to make the thickness of a diffusion layer formed by concentration polarization near the ion exchange membranes in the dilution chambers smaller than when air bubbles are not introduced thereto, and make the resistance between the electrodes smaller than when the air bubbles are not introduced thereto. Superficial air velocity of air bubbles introduced into the dilution chambers is preferably 1-4 cm/sec.
    • 在电透析过程中,该方法包括使电解液通过稀释室和形成在大量交替对置的阴离子交换膜和阳离子交换膜之间的浓缩室,并将电位施加到设置在 多个这样的室,从而通过膜将电解质中的离子迁移并进行电解质的稀释或浓缩,将这样的体积的气泡引入稀释室的下部,以形成扩散层的厚度 通过在稀释室内的离子交换膜附近的浓度偏振小于不引入气泡时的离子交换膜的极化浓度,并且使得电极之间的电阻小于不引入气泡时的电阻。 引入稀释室的气泡的表观空气速度优选为1-4cm / sec。
    • 10. 发明授权
    • Apparatus for separating living cells
    • 用于分离活细胞的装置
    • US5227066A
    • 1993-07-13
    • US722219
    • 1991-06-27
    • Masahiko IshidaRyoichi HagaHarumi Matsuzaki
    • Masahiko IshidaRyoichi HagaHarumi Matsuzaki
    • B04B5/00B04B15/12C12M1/26C12M3/00C12N1/02
    • B04B5/00B04B15/12C12M47/02C12N1/02Y10T436/111666Y10T436/25375
    • A centrifugal separation method for living cells is disclosed in which a cell containing solution is charged in a rotor chamber confined by a steam pressure-resistant wall. A rotor having septa is disposed in the rotor chamber. The septa are rotatable in the same direction at the same angular velocity as the rotor while at least part thereof is immersed in a solution in the rotor when cells are to be centrifuged from the cell-containing solution. After the cells are separated and adhered to the inner surface of the rotor, a supernatant in the rotor is discharged form the rotor. Then a fresh cultivating medium is introduced into the rotor, and the septa and the rotor are rotated at mutually different angular velocities while at least part of the septa is immersed in the fresh medium in the rotor. As a result, the cells are again suspended in the fresh medium.
    • 公开了一种用于活细胞的离心分离方法,其中将含细胞的溶液装入由耐蒸汽压壁限制的转子室中。 具有隔片的转子设置在转子室中。 隔垫可以以与转子相同的角速度在相同的方向上旋转,而当细胞从含细胞溶液中离心时,其至少部分浸入到转子的溶液中。 在细胞分离并粘附到转子的内表面之后,转子中的上清液从转子排出。 然后将新鲜的培养介质引入转子中,并且隔片和转子以相互不同的角速度旋转,同时至少部分隔片浸入转子中的新鲜介质中。 结果,细胞再次悬浮在新鲜培养基中。