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1. 发明授权

US07070951B2 Dewaxing kit for immunostaining tissue specimens 失效
标题翻译：用于免疫染色组织标本的脱蜡试剂盒
公开(公告)号：US07070951B2
公开(公告)日：2006-07-04
申请号：US10652854
申请日：2003-08-29
申请人： Guangrong Zhang , Cheng-Zhi Yu , Sheng-Hui Su , Krishan L. Kalra , Ding Zhou
发明人： Guangrong Zhang , Cheng-Zhi Yu , Sheng-Hui Su , Krishan L. Kalra , Ding Zhou
IPC分类号： G01N1/30
CPC分类号： C11D1/143 , C11D1/62 , C11D1/72 , C11D3/187 , C11D3/188 , C11D3/201 , C11D3/2044 , G01N1/30 , G01N1/36 , Y10S435/975
摘要： Compositions and methods are provided for dewaxing wax-embedded biological specimens prior to histochemical analysis. The compositions and methods provided can effectively remove wax or improved wax-based embedding materials, particularly paraffin-based, from specimens during preparation for histochemical or other diagnostic analyses, while minimizing danger to users, achieving compatibility with automated use, and maintaining compatibility with downstream histochemical analyses, particularly immunostaining. Compositions of the invention comprise a paraffin-solubilizing organic solvent, a polar organic solvent, and a surfactant. Compositions can further comprise water. The method involves contacting a wax-embedded specimen with the dewaxing composition to solubilize the wax impregnating the specimen prior to histochemical analysis. The method can comprise the further step of washing the dewaxed specimen immediately after dewaxing with an aqueous wash composition comprising a detergent to remove residual dewaxing composition. Also provided is a kit for dewaxing a wax-embedded specimen, which comprises a dewaxing composition and can further comprise a second composition of (1) an immunostaining reagent or (2) an aqueous wash solution comprising a detergent for removing residual dewaxing solution.
摘要翻译：提供组合物和方法，用于在组织化学分析前对蜡嵌入的生物样品进行脱蜡。所提供的组合物和方法可以在用于组织化学或其他诊断分析的准备期间有效地从标本中去除蜡或改进的蜡基嵌入材料，特别是基于石蜡的包埋材料，同时最小化对使用者的危害，实现与自动使用的兼容性并保持与下游的兼容性组织化学分析，特别是免疫染色。本发明的组合物包含石蜡溶解性有机溶剂，极性有机溶剂和表面活性剂。组合物还可以包含水。该方法包括使蜡包埋的样品与脱蜡组合物接触，以在组织化学分析之前溶解浸渍样品的蜡。该方法可以包括在用包含洗涤剂的水性洗涤组合物脱蜡之后立即洗涤脱蜡样品以除去残余脱蜡组合物的步骤。还提供了一种用于脱蜡蜡包埋样品的试剂盒，其包含脱蜡组合物，并且还可以包含（1）免疫染色试剂的第二组合物或（2）包含用于除去残余脱蜡溶液的洗涤剂的水性洗涤溶液。

2. 发明授权

US06632598B1 Deparaffinization compositions and methods for their use 失效
标题翻译：脱蜡组合物及其使用方法
公开(公告)号：US06632598B1
公开(公告)日：2003-10-14
申请号：US08212175
申请日：1994-03-11
申请人： Guangrong Zhang , Cheng-Zhi Yu , Sheng-Hui Su , Krishan L. Kalra , Ding Zhou
发明人： Guangrong Zhang , Cheng-Zhi Yu , Sheng-Hui Su , Krishan L. Kalra , Ding Zhou
IPC分类号： C12Q100
CPC分类号： C11D1/143 , C11D1/62 , C11D1/72 , C11D3/187 , C11D3/188 , C11D3/201 , C11D3/2044 , G01N1/30 , G01N1/36 , Y10S435/975
摘要： Compositions and methods are provided for dewaxing wax-embedded biological specimens prior to histochemical analysis. The compositions and methods provided can effectively remove wax or improved wax-based embedding materials, particularly paraffin-based, from specimens during preparation for histochemical or other diagnostic analyses, while minimizing danger to users, achieving compatibility with automated use, and maintaining compatibility with downstream histochemical analyses, particularly immunostaining. Compositions of the invention comprise a paraffin-solubilizing organic solvent, a polar organic solvent, and a surfactant. Compositions can further comprise water. The method involves contacting a wax-embedded specimen with the dewaxing composition to solubilize the wax impregnating the specimen prior to histochemical analysis. The method can comprise the further step of washing the dewaxed specimen immediately after dewaxing with an aqueous wash composition comprising a detergent to remove residual dewaxing composition. Also provided is a kit for dewaxing a wax-embedded specimen, which comprises a dewaxing composition and can further comprise a second composition of (1) an immunostaining reagent or (2) an aqueous wash solution comprising a detergent for removing residual dewaxing solution.
摘要翻译：提供组合物和方法，用于在组织化学分析前对蜡嵌入的生物样品进行脱蜡。所提供的组合物和方法可以在用于组织化学或其他诊断分析的准备期间有效地从标本中去除蜡或改进的蜡基嵌入材料，特别是基于石蜡的包埋材料，同时最小化对使用者的危害，实现与自动使用的兼容性并保持与下游的兼容性组织化学分析，特别是免疫染色。本发明的组合物包含石蜡溶解性有机溶剂，极性有机溶剂和表面活性剂。组合物还可以包含水。该方法包括使蜡包埋的样品与脱蜡组合物接触，以在组织化学分析之前溶解浸渍样品的蜡。该方法可以包括在用包含洗涤剂的水性洗涤组合物脱蜡之后立即洗涤脱蜡样品以除去残余脱蜡组合物的步骤。还提供了一种用于脱蜡蜡包埋样品的试剂盒，其包含脱蜡组合物，并且还可以包含第二组合物（1）免疫染色试剂或（2）包含用于除去残余脱蜡溶液的洗涤剂的水性洗涤溶液。 / PTEXT>

3. 再颁专利

USRE40835E1 Enhancement of immunochemical staining in aldehyde-fixed tissue 失效
标题翻译：增强醛固定组织中的免疫化学染色
公开(公告)号：USRE40835E1
公开(公告)日：2009-07-07
申请号：US11249180
申请日：1993-08-12
申请人： Shan-Rong Shi , Atul K. Tandon , Krishan L. Kalra , Nagesh Malhotra , Sheng-Hui Su , Cheng-Zhi Yu
发明人： Shan-Rong Shi , Atul K. Tandon , Krishan L. Kalra , Nagesh Malhotra , Sheng-Hui Su , Cheng-Zhi Yu
IPC分类号： G01N33/567
CPC分类号： G01N1/30 , G01N33/5306 , Y10T436/25125
摘要： A method for restoring immunoreactivity of a tissue, particularly decalcified tissue, fixed width an aldehyde fixative agent and embedded in an embedding medium, usually comprising celloidin, the method comprising the steps of contacting the tissue with an aldehyde releasing reagent solution comprising a solvent and an aldehyde releasing reagent and removing aldehyde released by the aldehyde releasing reagent from contact with the tissue by reacting the aldehyde in a substantially irreversible manner to form a non-aldehyde derivative, and removing excess base from the tissue. A preferred solution for celloidin-embedded decalcified tissue comprises methanolic sodium hydroxide at about one-third saturation.
摘要翻译：一种恢复组织，特别是脱钙组织的免疫反应性的方法，固定宽度的醛固定剂并包埋在通常包含肌内注射的包埋培养基中，所述方法包括以下步骤：使组织与包含溶剂的醛释放试剂溶液接触，醛释放试剂和通过使醛释放试剂释放的醛与基本上不可逆的方式反应而除去与组织接触的醛，以形成非醛衍生物，并从组织中除去多余的碱。对于胶原蛋白包埋的脱钙组织的优选溶液包含约三分之一饱和的甲醇氢氧化钠。

4. 发明授权

US5578452A Enhancement of immunochemical staining in aldehyde-fixed tissues 失效
标题翻译：增强醛固定组织中的免疫化学染色
公开(公告)号：US5578452A
公开(公告)日：1996-11-26
申请号：US211595
申请日：1994-04-07
申请人： Shan-Rong Shi , Atul K. Tandon , Krishan L. Kalra , Nagesh Malhotra , Sheng-Hui Su , Cheng-Zhi Yu
发明人： Shan-Rong Shi , Atul K. Tandon , Krishan L. Kalra , Nagesh Malhotra , Sheng-Hui Su , Cheng-Zhi Yu
IPC分类号： G01N1/30 , G01N33/53 , G01N33/567
CPC分类号： G01N1/30 , G01N33/5306 , Y10T436/25125
摘要： A method for restoring immunoreactivity of a tissue, particularly decalcified tissue, fixed width an aldehyde fixative agent and embedded in an embedding medium, usually comprising celloidin, the method comprising the steps of contacting the tissue with an aldehyde releasing reagent solution comprising a solvent and an aldehyde releasing reagent and removing aldehyde released by the aldehyde releasing reagent from contact with the tissue by reacting the aldehyde in a substantially irreversible manner to form a non-aldehyde derivative, and removing excess base from the tissue. A preferred solution for celloidin-embedded decalcified tissue comprises methanolic sodium hydroxide at about one-third saturation.
摘要翻译： PCT No.PCT / US93 / 07550 Sec。 371日期1994年4月7日 102（e）1994年4月7日PCT 1993年8月12日PCT PCT。公开号WO94 / 04906 日期1994年3月3日一种恢复组织，特别是脱钙组织的免疫反应性的方法，固定宽度的醛固定剂并包埋在通常包含肌内注射的包埋培养基中，该方法包括以下步骤：使组织与醛释放试剂溶液包括溶剂和醛释放试剂，并且通过使醛以基本上不可逆的方式反应形成非醛衍生物，并从组织中除去多余的碱，除去由醛释放试剂释放的醛与组织的接触。对于胶原蛋白包埋的脱钙组织的优选溶液包含约三分之一饱和的甲醇氢氧化钠。

5. 发明授权

US6130323A Non-nucleotide linking reagents 失效
标题翻译：非核苷酸连接试剂
公开(公告)号：US6130323A
公开(公告)日：2000-10-10
申请号：US171925
申请日：1998-10-26
申请人： Sheng-Hui Su , Rajkumar Siva Iyer , Sunil K. Aggarwal , Krishan L. Kalra
发明人： Sheng-Hui Su , Rajkumar Siva Iyer , Sunil K. Aggarwal , Krishan L. Kalra
IPC分类号： C12Q1/68 , C07F9/24 , C07F9/6561 , C07H21/00 , C07H19/00 , C07H21/02 , C07H21/04
CPC分类号： C07H21/00 , C07F9/2416 , C07F9/6561
摘要： Non-nucleotide reagents capable of forming oligomers with nucleotide units are disclosed, together with intermediates for synthesizing such non-nucleotide reagents, oligomers incorporating such reagents, kits containing such reagents and methods for their use in forming oligomers with nucleotide units.
摘要翻译： PCT No.PCT / US97 / 09094 Sec。 371日期：1998年10月26日 102（e）日期1998年10月26日PCT提交1997年5月15日PCT公布。第WO97 / 43451号公报日本1997年11月20日公开了能够形成具有核苷酸单元的寡聚体的非核苷酸试剂，以及用于合成这种非核苷酸试剂的中间体，含有此类试剂的低聚物，含有此类试剂的试剂盒及其用于形成具有核苷酸单元的寡聚体。

6. 发明授权

US5916750A Multifunctional linking reagents for synthesis of branched oligomers 失效
标题翻译：用于合成支链低聚物的多官能连接试剂
公开(公告)号：US5916750A
公开(公告)日：1999-06-29
申请号：US780725
申请日：1997-01-08
申请人： Rajkumar Siva Iyer , Sheng-Hui Su , Anita Inamdar , Krishan L. Kalra
发明人： Rajkumar Siva Iyer , Sheng-Hui Su , Anita Inamdar , Krishan L. Kalra
IPC分类号： C12N15/09 , C07D251/34 , C07D495/04 , C07F9/6521 , C07H21/00 , C07H21/02 , C07H21/04 , C12Q1/68 , G01N33/543 , C07D413/00
CPC分类号： C07H21/00
摘要： Reagents capable of forming branched oligomers with monomeric units are disclosed, together with oligomers incorporating such reagents, kits containing such reagents and methods for use of such reagents in forming oligomers with monomeric units. The present reagents can advantageously be used to introduce multiple labels or reporter molecules onto oligomers such as oligonucleotides and oligopeptides. In particular, non-nucleosidic phosphoramidites based on 1,3,5-tris(2-hydroxyethyl)cyanuric acid are disclosed. Multiply-labeled, branched DNA oligomer probes constructed using these phosphoramidite reagents showed increased signal intensity relative to singly-labeled oligomer probes.
摘要翻译：公开了能够形成具有单体单元的支链低聚物的试剂，连同含有此类试剂的低聚物，含有这些试剂的试剂盒和用于形成具有单体单元的低聚物的这种试剂的方法。本发明的试剂可有利地用于将多个标记或报告分子引入低聚物如寡核苷酸和寡肽。特别地，公开了基于1,3,5-三（2-羟乙基）氰尿酸的非核苷亚磷酰胺。使用这些亚磷酰胺试剂构建的多重标记的支链DNA寡聚体探针相对于单标记的寡聚体探针显示增加的信号强度。

7. 发明授权

US06451551B1 Releasing embedding media from tissue specimens 失效
标题翻译：从组织标本中释放嵌入介质
公开(公告)号：US06451551B1
公开(公告)日：2002-09-17
申请号：US09573073
申请日：2000-05-16
申请人： Guangrong Zhan , Krishan L. Kalra , Sheng-Hui Su , Taiying Chen
发明人： Guangrong Zhan , Krishan L. Kalra , Sheng-Hui Su , Taiying Chen
IPC分类号： G01N130
CPC分类号： G01N1/36 , G01N1/30
摘要： Releasing the embedding medium from an embedded histochemically reactive tissue specimen is provided by contacting the embedded tissue specimen with a releasing composition under conditions sufficient to release a sufficient portion of the embedding medium associated with the histochemically reactive tissue specimen to permit analysis without substantial adverse effect on the histochemical reactivity of the specimen.
摘要翻译：通过使嵌入的组织样本与释放组合物在足以释放与组织化学反应性组织标本相关的足够部分的包埋培养基的条件下接触来提供嵌入介质，以允许分析，而不会对样品的组织化学反应性。

8. 发明授权

US5948359A Automated staining apparatus 失效
标题翻译：自动染色装置
公开(公告)号：US5948359A
公开(公告)日：1999-09-07
申请号：US820883
申请日：1997-03-21
申请人： Krishan L. Kalra , Jason Z. Zhang , Zhi-Weng Chang , Jianghong Shui
发明人： Krishan L. Kalra , Jason Z. Zhang , Zhi-Weng Chang , Jianghong Shui
IPC分类号： G01N1/31 , G01N35/10
CPC分类号： G01N35/109 , G01N1/312 , G01N2001/317 , Y10T436/11 , Y10T436/112499 , Y10T436/119163 , Y10T436/2575
摘要： Disclosed is an automated staining apparatus including an arm moveable in three dimensions, and a hollow tip head located on the arm including integral reagent tip head, wash tip and blow tip for selectively dispensing gas and liquid onto microscope slides. Also disclosed are various sub-components of the apparatus that are specifically adapted to the processing of specimens on slides.
摘要翻译：公开了一种自动染色装置，其包括可移动三维的臂和位于臂上的中空尖头，其包括整体的试剂尖头，清洗尖和吹头，用于将气体和液体选择性地分配到显微镜载玻片上。还公开了该设备的各种子部件，其特别适用于在载玻片上处理样品。

9. 发明授权

US09551635B2 Sample processing system 有权
标题翻译：样品处理系统
公开(公告)号：US09551635B2
公开(公告)日：2017-01-24
申请号：US12542646
申请日：2009-08-17
申请人： Krishan L. Kalra , Wallace Chang , Ravishankar Melkote
发明人： Krishan L. Kalra , Wallace Chang , Ravishankar Melkote , Shazi Iqbal
IPC分类号： G01N1/28 , B01L3/00 , G01N1/31 , B01L7/00 , G01N35/00 , G01N35/10
CPC分类号： G01N1/28 , B01L3/508 , B01L3/5085 , B01L7/52 , B01L2200/0689 , B01L2200/147 , B01L2300/022 , B01L2300/024 , B01L2300/041 , B01L2300/0822 , G01N1/312 , G01N35/0099 , G01N35/109 , G01N2001/317 , G01N2035/00752 , Y10S901/31
摘要： In accordance with an embodiment of a system for handling and processing chemical and/or biological samples, a MicroChamber comprises a substrate, a reservoir formed on the substrate for receiving a chemical and/or biological sample, and an encoder such as a barcode or other suitable device. The encoder encodes information describing at least one characteristic of the substrate and/or reservoir.
摘要翻译：根据用于处理和处理化学和/或生物样品的系统的实施例，MicroChamber包括基板，形成在基板上的用于接收化学和/或生物样品的储存器，以及编码器，例如条形码或其它合适的装置。编码器编码描述衬底和/或贮存器的至少一个特性的信息。

10. 发明授权

US07472803B2 Systems and methods for dispensing objects 失效
标题翻译：用于分配物体的系统和方法
公开(公告)号：US07472803B2
公开(公告)日：2009-01-06
申请号：US11067657
申请日：2005-02-25
申请人： Krishan L. Kalra , Thomas Maxwell , Qi Cao , Michael Huang , Shahin Iqbal
发明人： Krishan L. Kalra , Thomas Maxwell , Qi Cao , Michael Huang , Shahin Iqbal
IPC分类号： B65G59/06
CPC分类号： B65H1/06 , B65H3/24
摘要： An automated dispensing assembly includes a base, and a shuttle mounted in the base and movable under automated control between a loading position and a dispensing position. The shuttle includes a cavity configured to carry an object, such as cover. The depth of the cavity in the shuttle is approximately the same (or less) as the thickness of one of the objects. A storage module is mounted proximate the shuttle. The storage module is configured to store a plurality of objects and includes an opening exposing the next object to be dispensed. The cavity is positioned adjacent the opening in the storage module in the loading position and an edge of the cavity separates the object to be dispensed from the other objects in the storage module as the shuttle moves to the dispensing position.
摘要翻译：自动分配组件包括基座和安装在基座中并可在装载位置和分配位置之间的自动控制下移动的梭。梭子包括被配置为承载物体（例如盖）的空腔。梭子中空腔的深度与其中一个物体的厚度大致相同（或更小）。存放模块靠近梭子安装。存储模块被配置为存储多个对象，并且包括露出下一个待分配对象的开口。空腔在加载位置处邻近存储模块中的开口定位，并且当梭子移动到分配位置时，腔的边缘将待分配的物体与存储模块中的其他物体分离。

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