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1. 发明授权

US06384912B1 Measuring device and the use thereof 有权
公开(公告)号：US06384912B1
公开(公告)日：2002-05-07
申请号：US09742391
申请日：2000-12-22
申请人： Gerolf Kraus , Michael Pawlak , Gert Ludwig Duveneck , Peter Oroszlan , Andreas Helg , Alfredo Emilio Bruno-Raimondi
发明人： Gerolf Kraus , Michael Pawlak , Gert Ludwig Duveneck , Peter Oroszlan , Andreas Helg , Alfredo Emilio Bruno-Raimondi
IPC分类号： G01N110
摘要： A device has a planar optical waveguide, including a transparent carrier (40) and a wave-guiding layer (41), wherein the waveguide at least has a diffractive element (42) for coupling excitation radiation into the wave-guiding layer. Located on the wave-guiding layer is a further, tightly sealing layer (43) made from a material which, at least at the support surface, is transparent both to the excitation radiation and to the evanescently excited radiation at least to the penetration depth of the evanescent field, and which, for an analysis sample, has, at least in a partial region of the guided excitation radiation, a cavity (45) which is open to the upper side or a cavity (6) which is closed to the upper side and connected by means of an inlet channel (2) and an outlet channel (3). The depth of the cavity corresponds at least to the penetration depth of the evanescent field, and the diffractive element (42) is fully covered by the material of the layer (43) at least in the coupling-in region of the excitation radiation.

2. 发明授权

US06198869B1 Measuring device and its use 有权
标题翻译：测量装置及其用途
公开(公告)号：US06198869B1
公开(公告)日：2001-03-06
申请号：US09308096
申请日：1999-05-17
申请人： Gerolf Kraus , Michael Pawlak , Gert Ludwig Duveneck , Peter Oroszlan , Andreas Helg , Alfredo Emilio Bruno-Raimondi
发明人： Gerolf Kraus , Michael Pawlak , Gert Ludwig Duveneck , Peter Oroszlan , Andreas Helg , Alfredo Emilio Bruno-Raimondi
IPC分类号： G01N2100
CPC分类号： G01N21/648 , G01N21/05 , G01N21/7703 , G01N2021/0346
摘要： A device comprising a planar optical waveguide, consisting of a transparent carrier (40) and a wave-guiding layer (41), wherein the waveguide at least has a diffractive element (42) for coupling excitation radiation into the wave-guiding layer, and there is located on the wave-guiding layer a further, tightly sealing layer (43) made from a material which, at least at the support surface, is transparent both to the excitation radiation and to the evanescently excited radiation at least to the penetration depth of the evanescent field, and which, for an analysis sample, has, at least in a partial region of the guided excitation radiation, a cavity (45) which is open to the upper side or a cavity (6) which is closed to the upper side and connected by means of an inflow channel (2) and an outflow channel (3), the depth of the cavity corresponding at least to the penetration depth of the evanescent field, and wherein the diffractive element (42) is fully covered by the material of the layer (43) at least in the coupling-in region of the excitation radiation.
摘要翻译：一种包括由透明载体（40）和波导层（41）组成的平面光波导的装置，其中所述波导至少具有用于将激发辐射耦合到波导层中的衍射元件（42），以及位于波导层上的另一紧密密封层（43）由材料制成，所述材料至少在支撑表面上对于激发辐射和至少到穿透深度的消逝的辐射是透明的，并且对于分析样本，至少在被引导的激发辐射的部分区域中具有向上侧敞开的空腔（45）或与所述空腔（6）关闭的空腔（6），所述空腔上侧并且通过流入通道（2）和流出通道（3）连接，所述空腔的深度至少对应于所述ev逝场的穿透深度，并且其中所述衍射元件（42）完全被层的材料（43）至少在激发辐射的耦合区域中。

3. 发明申请

US20050059014A1 Analytical platform and detection method with the analytes to be determined in a sample as immobilized specific binding partners 审中-公开
标题翻译：分析平台和检测方法与样品中待测定的分析物作为固定化的特异性结合配偶体
公开(公告)号：US20050059014A1
公开(公告)日：2005-03-17
申请号：US10653386
申请日：2003-09-03
申请人： Michael Pawlak , Eginhard Schick , Peter Oroszlan
发明人： Michael Pawlak , Eginhard Schick , Peter Oroszlan
IPC分类号： G01N33/566 , C12M1/00 , C12M1/34 , C12Q1/68 , G01N21/76 , G01N33/53 , G01N33/543 , G01N33/553
CPC分类号： G01N33/54353 , G01N33/54373
摘要： The present invention is related to an analytical platform and a method performed therewith for the analysis of a multitude of “nature-identical” samples for analytes which are contained therein and are of biological relevance as binding partners in specific binding reactions, wherein said samples or dilutions of said samples of the same relative molecular composition as the original samples, with the analytes to be determined contained therein, as a first plurality of specific binding partners, are deposited in discrete measurement areas in at least one one- or two-dimensional array of measurement areas on an evanescent field sensor platform as a solid support without a change in the relative molecular composition, compared with the original relative molecular composition of the sample, one or more tracer compounds as a second plurality of specific binding partners, for the specific determination of analytes out of the first plurality of specific binding partners contained in the samples, are brought into contact with the samples deposited in said discrete measurement areas in a single step or multiple steps of a specific binding reaction, changes in opto-electronic signals, resulting from the binding of tracer compounds to analytes contained in the samples in discrete measurement areas or to specific binding partners bound to the analytes contained in the measurement areas in the evanescent field of the evanescent field sensor platform are measured laterally resolved, and the presence of the analytes to be specifically detected is determined qualitatively and/or quantitatively from the relative amount of the changes in said opto-electronic signals from the corresponding measurement areas.
摘要翻译：本发明涉及一种分析平台及其分析方法，用于分析大量与分析物相似的“自然相同”的样品，其中所含的分析物样品在其中包含并具有生物相关性，作为特异性结合反应中的结合配偶体，其中所述样品或与原始样品相同的相对分子组成的所述样品与其中包含的待测定的分析物作为第一多个特异性结合配偶体的稀释沉积在至少一个一维或二维阵列的离散测量区域中相对于样品的原始相对分子组成，作为第二多个特异性结合配偶体的一个或多个示踪剂化合物，作为固体支持物的ev逝场传感器平台上的测量区域作为固体支持物而没有相对分子组成的变化确定包含在第一个多个具体约束伙伴中的分析物在与单独的步骤或特定结合反应的多个步骤中，沉积在所述离散测量区域中的样品与示踪剂化合物与样品中包含的分析物的离散性的光电子信号的变化相接触测量区域或与消逝场传感器平台的消逝场中测量区域中包含的分析物结合的特异性结合配体进行横向分辨测定，并且特异性检测的分析物的存在是从定性和/或定量的相应测量区域的所述光电信号的相对变化量。

4. 发明申请

US20080020409A1 Analytical Platform and Method for Generating Protein Expression Profiles of Cell Populations 审中-公开
标题翻译：分析平台和生成细胞群体蛋白质表达谱的方法
公开(公告)号：US20080020409A1
公开(公告)日：2008-01-24
申请号：US10598418
申请日：2004-03-03
申请人： Michael Pawlak , Eginhard Schick , Peter Oroszlan
发明人： Michael Pawlak , Eginhard Schick , Peter Oroszlan
IPC分类号： G01N33/574 , C12M1/34 , C40B30/04 , G01N33/567 , G01N33/569
CPC分类号： G01N33/6845 , G01N33/567 , G01N33/6842
摘要： The present invention is related to analytical platforms and methods performed therewith for generating qualitative and/or quantitative protein expression profiles, in particular differential protein expression profiles, of cell populations comprising: generating lysates of one or more populations of cells, the lysates comprising a plurality of proteins expressed by the respective cell populations, providing an essentially planar solid support, depositing at discrete sites small quantities of the cell lysates, in diluted or undiluted form directly on said solid support or on an adhesion-promoting layer applied on said solid support, thereby creating one or more one- or two-dimensional arrays of discrete measurement areas on said solid support, applying a number of binding reagents as specific binding partners for the proteins contained in cell lysates in discrete measurement areas and to be detected and, if adequate, one or more detection reagents on said one or more arrays of measurement areas, the binding reagents and the detection reagents being applied sequentially or in a single addition-step, after binding of the detection reagents to the binding reagents, to the one or more arrays of discrete measurement areas for e.g. global analysis of signaling pathways or screening antibody sets/libraries against protein targets for best specificity, selectivity and affinity, and measuring and recording optical signals emanating from said one or more arrays of discrete measurement areas in a locally resolved manner, wherein said essentially planar solid support is non-porous and an optionally applied adhesion-promoting layer has a thickness of less than 1 μm.
摘要翻译：本发明涉及用于产生细胞群体的定性和/或定量蛋白质表达谱，特别是差异蛋白质表达谱的分析平台和方法，其包括：产生一个或多个细胞群的裂解物，裂解物包含多个由相应的细胞群体表达的蛋白质，提供基本上平面的固体支持物，在稀释或未稀释的形式的离散位置沉积在所述固体支持物上或施加在所述固体支持物上的粘附促进层上，从而在所述固体支持物上产生离散测量区域的一个或多个一维或二维阵列，将多个结合试剂作为离散测量区域中包含在细胞裂解物中的蛋白质的特异性结合配偶体，并且如果足够，所述一个或多个测量阵列上的一种或多种检测试剂在将检测试剂与结合试剂结合后，顺序地或在单个添加步骤中将结合试剂和检测试剂施用到例如离子测量区域的一个或多个阵列。全面分析信号通路或筛选针对蛋白质靶标的抗体集/文库，以获得最佳特异性，选择性和亲和力，以及以局部分辨方式测量和记录从所述一个或多个离散测量区域阵列发出的光信号，其中所述基本上平面的固体支撑体是无孔的，并且任选地施加的粘附促进层的厚度小于1μm。

5. 发明申请

US20050163659A1 Kit for assay development and serial analysis 审中-公开
标题翻译：用于测定开发和连续分析的试剂盒
公开(公告)号：US20050163659A1
公开(公告)日：2005-07-28
申请号：US10514166
申请日：2003-05-06
申请人： Gert Duveneck , Peter Oroszlan , Michael Pawlak
发明人： Gert Duveneck , Peter Oroszlan , Michael Pawlak
IPC分类号： G01N33/543 , G01N21/00
CPC分类号： G01N33/54353 , G01N33/54366
摘要： The invention relates to a kit for assay development and for carrying out a plurality of analyses, comprising a carrier substrate and a placement body jointly forming an arrangement of a plurality of sample compartments comprising said carrier substrate as a base plate, in addition to a plurality of immobilized binding partners for the detection of one or more analytes in one or more samples in a bioaffinity assay, said binding partners being arranged and immobilized on the carrier substrate inside the sample containers always in two-dimensional arrays of discrete measuring areas, wherein always at least one measuring area of an array or a partial surface inside an array or sample compartment, respectively, is provided on the carrier substrate for referencing purposes, and the surface density of the immobilized binding partners, in relation to the surface of the measurement areas, is less than the surface density of a full, i.e. extensive, monolayer of said binding partners. The composition of the inventive kit is such that, surprisingly, it enables a full series of measurements to be carried out on an individual carrier substrate. The invention also relates to an analytical system wherein the inventive kit is used, and to analytical detection methods based thereon and the use thereof.
摘要翻译：本发明涉及用于测定开发和用于进行多个分析的试剂盒，其包括载体基底和放置体，该载体基底和放置体除了多个之外，共同形成包括所述载体基底作为基板的多个样品室的布置用于在生物亲和力测定中检测一个或多个样品中的一种或多种分析物的固定化结合配偶体，所述结合伙伴被布置和固定在样品容器内的载体基质上，总是在离散测量区域的二维阵列中，其中总是为了参考目的，在载体基底上分别提供阵列或样品室内的阵列或部分表面的至少一个测量区域，并且固定的结合物的表面密度相对于测量区域的表面小于所述结合配偶体的完整的，即广泛的单层的表面密度。本发明的试剂盒的组成使得令人惊奇的是，它使得能够在单个载体基底上进行全系列的测量。本发明还涉及其中使用本发明的试剂盒的分析系统，以及基于其的分析检测方法及其用途。

6. 发明申请

US20060188873A1 Method for detecting a target polynucleotide 审中-公开
标题翻译：检测靶多核苷酸的方法
公开(公告)号：US20060188873A1
公开(公告)日：2006-08-24
申请号：US11246287
申请日：2005-10-11
申请人： Andreas Abel , James Beck , Markus Ehrat , Peter Oroszlan
发明人： Andreas Abel , James Beck , Markus Ehrat , Peter Oroszlan
IPC分类号： C12Q1/70 , C12Q1/68 , C12M1/34 , G02B6/00
CPC分类号： C12Q1/682 , C12Q1/6825 , C12Q2565/607 , C12Q2537/125 , C12Q2565/501
摘要： A method of detecting a target polynucleotide using a solution phase nucleic acid sandwich assay, wherein the solid support is an optical planar waveguide, and the label is detected by measuring the luminescence that is excited in the evanescent field of the waveguide-A fully automated system may be used. The method may be of use in detecting nucleic acids associated with disease at very low concentrations.
摘要翻译：使用溶液相核酸夹心测定法检测靶多核苷酸的方法，其中固体支持物是光学平面波导，并且通过测量在波导-A全自动系统的消逝场中激发的发光来检测标记可能用过了。该方法可用于以非常低的浓度检测与疾病相关的核酸。

7. 发明授权

US5741639A Device and method for combined bioaffinity assay and electrophoretic separation of multiple analytes 失效
标题翻译：用于组合生物亲和测定和多分析物电泳分离的装置和方法
公开(公告)号：US5741639A
公开(公告)日：1998-04-21
申请号：US396310
申请日：1995-02-28
申请人： Kees Ensing , Peter Oroszlan , Aran Paulus , Carlo S. Effenhauser
发明人： Kees Ensing , Peter Oroszlan , Aran Paulus , Carlo S. Effenhauser
IPC分类号： G01N33/543 , G01N27/447 , G01N33/561 , C12Q1/68
CPC分类号： G01N33/561 , Y10S435/96 , Y10S435/961 , Y10S436/81
摘要： A device for combined bioaffinity assay and electrophoretic separation is provided, which comprises a capillary system having two stages, a first stage in which bioaffinity interactions of analyte molecules and molecular recognition elements are performed, and a second stage, in which electrophoretic separation of the analyte molecules and subsequent detection of the separated species is accomplished. Within the first capillary stage the molecular recognition elements are attached and immobilized to the inside capillary wall, for example, by adsorption or by covalent binding to the capillary material. The method for combined bioaffinity assay and electrophoretic separation comprises flowing an analyte through a capillary system having two stages. In a first capillary stage the analyte molecules are captured by respective molecular recognition elements present in that stage. More particularly the analyte molecules are captured by molecular recognition elements which are attached and immobilized to the inside wall of that capillary stage, for example, by adsorption or by covalent binding to the capillary material. After a predetermined time the analyte-molecules are dissociated from the molecular recognition elements. Subsequently the analyte-molecules are separated in a second stage of the capillary system by capillary electrophoresis and finally the separated species are detected at the terminal part of the capillary system.
摘要翻译：提供了用于组合生物亲和测定和电泳分离的装置，其包括具有两个阶段的毛细管系统，其中进行分析物分子和分子识别元件的生物亲和力相互作用的第一阶段，以及分析物的电泳分离的第二阶段分子和随后的分离物质的检测是完成的。在第一毛细血管阶段内，例如通过吸附或通过与毛细管材料的共价结合将分子识别元件附着并固定到内部毛细管壁。用于组合生物亲和测定和电泳分离的方法包括使分析物流过具有两个阶段的毛细管系统。在第一毛细血管阶段，分析物分子被该阶段中存在的相应的分子识别元件捕获。更具体地，分析物分子被分子识别元件捕获，分子识别元件例如通过吸附或通过与毛细管材料的共价结合而附着和固定到该毛细管阶段的内壁。在预定时间后，分析物 - 分子与分子识别元件分离。随后，通过毛细管电泳在毛细管系统的第二阶段分离分析物分子，最后在毛细管系统的末端部分检测分离的物质。

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