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1. 发明授权

US5741639A Device and method for combined bioaffinity assay and electrophoretic separation of multiple analytes 失效
标题翻译：用于组合生物亲和测定和多分析物电泳分离的装置和方法
公开(公告)号：US5741639A
公开(公告)日：1998-04-21
申请号：US396310
申请日：1995-02-28
申请人： Kees Ensing , Peter Oroszlan , Aran Paulus , Carlo S. Effenhauser
发明人： Kees Ensing , Peter Oroszlan , Aran Paulus , Carlo S. Effenhauser
IPC分类号： G01N33/543 , G01N27/447 , G01N33/561 , C12Q1/68
CPC分类号： G01N33/561 , Y10S435/96 , Y10S435/961 , Y10S436/81
摘要： A device for combined bioaffinity assay and electrophoretic separation is provided, which comprises a capillary system having two stages, a first stage in which bioaffinity interactions of analyte molecules and molecular recognition elements are performed, and a second stage, in which electrophoretic separation of the analyte molecules and subsequent detection of the separated species is accomplished. Within the first capillary stage the molecular recognition elements are attached and immobilized to the inside capillary wall, for example, by adsorption or by covalent binding to the capillary material. The method for combined bioaffinity assay and electrophoretic separation comprises flowing an analyte through a capillary system having two stages. In a first capillary stage the analyte molecules are captured by respective molecular recognition elements present in that stage. More particularly the analyte molecules are captured by molecular recognition elements which are attached and immobilized to the inside wall of that capillary stage, for example, by adsorption or by covalent binding to the capillary material. After a predetermined time the analyte-molecules are dissociated from the molecular recognition elements. Subsequently the analyte-molecules are separated in a second stage of the capillary system by capillary electrophoresis and finally the separated species are detected at the terminal part of the capillary system.
摘要翻译：提供了用于组合生物亲和测定和电泳分离的装置，其包括具有两个阶段的毛细管系统，其中进行分析物分子和分子识别元件的生物亲和力相互作用的第一阶段，以及分析物的电泳分离的第二阶段分子和随后的分离物质的检测是完成的。在第一毛细血管阶段内，例如通过吸附或通过与毛细管材料的共价结合将分子识别元件附着并固定到内部毛细管壁。用于组合生物亲和测定和电泳分离的方法包括使分析物流过具有两个阶段的毛细管系统。在第一毛细血管阶段，分析物分子被该阶段中存在的相应的分子识别元件捕获。更具体地，分析物分子被分子识别元件捕获，分子识别元件例如通过吸附或通过与毛细管材料的共价结合而附着和固定到该毛细管阶段的内壁。在预定时间后，分析物 - 分子与分子识别元件分离。随后，通过毛细管电泳在毛细管系统的第二阶段分离分析物分子，最后在毛细管系统的末端部分检测分离的物质。

2. 发明授权

US08907059B2 Phosphopeptide enrichment of compositions by fractionation on ceramic hydroxyapatite 有权
$Phosphopeptide enrichment of compositions by fractionation on ceramic hydroxyapatite$
标题翻译：通过分级在陶瓷羟基磷灰石上的磷酸化富集组合物
公开(公告)号：US08907059B2
公开(公告)日：2014-12-09
申请号：US12615080
申请日：2009-11-09
申请人： Ning Liu , Katrina Academia , Aran Paulus , Tim Wehr , John A. Walker, II , Steve Freeby
发明人： Ning Liu , Katrina Academia , Aran Paulus , Tim Wehr , John A. Walker, II , Steve Freeby
IPC分类号： C07K1/14 , B01J20/04 , B01D15/38 , B01J20/282 , C07K1/16
CPC分类号： B01J20/048 , B01D15/3847 , B01J20/282 , C07K1/14 , C07K1/165
摘要： Phosphorylated peptides are extracted from digests of biological liquids and other peptide mixtures by fractionation on ceramic hydroxyapatite. The ceramic hydroxyapatite is readily usable in a centrifuge, allowing for rapid fractionations of a large number of small volume samples, and accordingly high throughput.
摘要翻译：通过在陶瓷羟基磷灰石上分级，从生物液体和其他肽混合物的消化物中提取磷酸化肽。陶瓷羟基磷灰石易于在离心机中使用，允许大量小体积样品的快速分级，并因此高通量。

3. 发明授权

US5965237A Microstructure device 失效
标题翻译：微结构器件
公开(公告)号：US5965237A
公开(公告)日：1999-10-12
申请号：US954998
申请日：1997-10-20
申请人： Gerardus Jacobus Maria Bruin , Carlo Stefan Effenhauser , Andreas Helg , Aran Paulus
发明人： Gerardus Jacobus Maria Bruin , Carlo Stefan Effenhauser , Andreas Helg , Aran Paulus
IPC分类号： B25J7/00 , B32B3/00
CPC分类号： B25J7/00 , Y10S366/03 , Y10T428/24479 , Y10T428/24612 , Y10T428/24661 , Y10T428/24744 , Y10T428/31663
摘要： An improved microstructure for micro-scale fluid handling, processing and investigation comprises a support element and a microstructured element. The support element has a substantially even support face. The microstructured element has a microstructured face provided both with substantially even portions and with recessed portions. The even portions are substantially coplanar to a common even surface. The recessed portions are defined between the even portions adjacent thereto and recessed from the common even surface. The support face is located substantially adjacent to the microstructured face and cooperates with the recessed portions thereof to define a microchannel system. At least the microstructured element is made and formed of poly(dimethylsiloxane) polymer by applying a prepolymer or a precursor thereof onto a corresponding master element, and then curing the material.
摘要翻译：用于微尺度流体处理，处理和研究的改进的微结构包括支撑元件和微结构元件。支撑元件具有基本均匀的支撑面。微结构元件具有设置有基本均匀的部分和凹陷部分的微结构化面。平均部分基本上与共同的均匀表面共面。凹部被限定在与其相邻的偶数部分之间并且从共同的均匀表面凹进。支撑面基本上邻近于微结构化面并且与其凹部配合以限定微通道系统。至少微结构元件由聚（二甲基硅氧烷）聚合物制成并通过将预聚物或其前体施加到相应的母体上，然后固化该材料而形成。

4. 发明申请

US20130220830A1 ELECTRONIC CONTROL OF PH AND IONIC STRENGTH 有权
标题翻译： PH和离子强度的电子控制
公开(公告)号：US20130220830A1
公开(公告)日：2013-08-29
申请号：US13768253
申请日：2013-02-15
申请人： Aran Paulus , Camille Diges , Roumen Bogoev , Sricharan Bandhakavi , Annett Hahn-Windgassen , Anton Posch , Elad Brod , Uri Sivan
发明人： Aran Paulus , Camille Diges , Roumen Bogoev , Sricharan Bandhakavi , Annett Hahn-Windgassen , Anton Posch , Elad Brod , Uri Sivan
IPC分类号： G01N30/06 , C25B9/10
CPC分类号： G01N30/06 , B01D57/02 , B01D61/445 , B01D61/46 , B01D61/50 , B01D2311/18 , C25B9/10 , G01N30/96
摘要： Apparatuses and methods for controlling ionic strength and/or pH of a solution are provided.
摘要翻译：提供了用于控制溶液的离子强度和/或pH值的装置和方法。

5. 发明授权

US06379515B1 Process for separating mixtures of substances using capillary affinity gel electrophoresis 有权
标题翻译：使用毛细管亲和凝胶电泳分离物质混合物的方法
公开(公告)号：US06379515B1
公开(公告)日：2002-04-30
申请号：US09180984
申请日：1998-11-19
申请人： Angelika Muscate-Magnussen , Aran Paulus , François Natt
发明人： Angelika Muscate-Magnussen , Aran Paulus , François Natt
IPC分类号： G01N2726
CPC分类号： G01N27/44747
摘要： Process for the selective separation of electrically charged target molecules in an analytical mixture by means of capillary affinity gel electrophoresis, using a capillary tube which is at least partly filled with a polymer gel, whereby receptors for target molecules are covalently bound to the polymer, and an electric field of at least 50 volts/cm is applied, characterized in that (a) the capillary tube is charged with the analytical mixture, (b) in a first separation stage the target molecules in the analytical mixture are bound to the receptors and the remaining components are eluted, optionally while splitting open, and (c) in a second stage of the process the elution conditions are changed, optionally in stages, so that the affinity of the target molecules for the receptor is eliminated and the target molecules are eluted and detected, optionally whilst splitting open.
摘要翻译：通过毛细管亲和性凝胶电泳，使用至少部分填充有聚合物凝胶的毛细管选择性分离分析混合物中的带电荷的靶分子的方法，由此靶分子的受体共价结合到聚合物上，以及施加至少50伏/厘米的电场，其特征在于（a）毛细管装有分析混合物，（b）在第一分离阶段，分析混合物中的靶分子与受体结合，洗脱剩余的组分，任选地分开开放，和（c）在该过程的第二阶段中，洗脱条件可任选地分阶段改变，从而消除靶分子对受体的亲和力，靶分子是洗脱和检测，任选地同时分开开放。

6. 发明授权

US4898658A Integrated temperature control/alignment system for high performance capillary electrophoretic apparatus 失效
标题翻译：用于高性能毛细管电泳装置的集成温度控制/对准系统
公开(公告)号：US4898658A
公开(公告)日：1990-02-06
申请号：US125539
申请日：1987-11-25
申请人： Barry L. Karger , Aran Paulus , Aharon S. Cohen , Robert J. Nelson
发明人： Barry L. Karger , Aran Paulus , Aharon S. Cohen , Robert J. Nelson
IPC分类号： F25B21/04 , G01N27/447
CPC分类号： G01N27/44708 , F25B21/04 , G01N27/44704
摘要： An integrated temperature control/alignment system for use with a high performance capillary electrophoretic apparatus is disclosed. In one exemplary embodiment the integrated temperature control/alignment system comprises a pair of capillary column mounting plates for mounting a capillary column as a constituent of the electrophoretic apparatus, a pair of secondary support plates, a pair of thermoelectric plates and external heat sink plates. Detection openings are formed in each of the plates as required so that a continuous detection path exists through the integrated temperature control/alignment system. The capillary column is seated and locked in grooves formed in the capillary column mounting plates so that the detection windows of the capillary column are coordinated with the aligned detection openings formed in the applicable plates of the integrated temperature control/alignment system. The temperature of substantially the entire capillary column, including the detection zone, is controlled or regulated by the temperatures of the thermoelectric plates which comprise one or more thermoelectric circuits utilizing the Peltier effect. Regulation of current flow in the thermoelectric plates controls the temperature effect exerted by the thermoelectric plates, thereby controlling both the direction and rate of heat transfer with the capillary column.

7. 发明申请

US20100125128A1 PHOSPHOPEPTIDE ENRICHMENT OF COMPOSITIONS BY FRACTIONATION ON CERAMIC HYDROXYAPATITE 有权
标题翻译：通过分解在陶瓷羟基磷灰石上的组合物的磷酸盐增殖
公开(公告)号：US20100125128A1
公开(公告)日：2010-05-20
申请号：US12615080
申请日：2009-11-09
申请人： Ning Liu , Katrina Academia , Aran Paulus , Tim Wehr , John A. Walker, II , Steve Freeby
发明人： Ning Liu , Katrina Academia , Aran Paulus , Tim Wehr , John A. Walker, II , Steve Freeby
IPC分类号： C07K1/14
CPC分类号： B01J20/048 , B01D15/3847 , B01J20/282 , C07K1/14 , C07K1/165
摘要： Phosphorylated peptides are extracted from digests of biological liquids and other peptide mixtures by fractionation on ceramic hydroxyapatite. The ceramic hydroxyapatite is readily usable in a centrifuge, allowing for rapid fractionations of a large number of small volume samples, and accordingly high throughput.
摘要翻译：通过在陶瓷羟基磷灰石上分级，从生物液体和其他肽混合物的消化物中提取磷酸化肽。陶瓷羟基磷灰石易于在离心机中使用，允许大量小体积样品的快速分级，并因此高通量。

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